期刊
JOURNAL OF CELLULAR PHYSIOLOGY
卷 236, 期 6, 页码 4709-4724出版社
WILEY
DOI: 10.1002/jcp.30191
关键词
3D in vitro culture; breast cancer; chemotherapy; decellularized scaffold; extracellular matrix; tumor microenvironment
资金
- Stiftelsen Assar Gabrielssons Fond [FB18-43, FB19-15]
- Swedish Cancer Foundation [PjF 20 0306, 2016-438, 2016-486]
- Knut och Alice Wallenbergs Stiftelse
- Stiftelsen for Strategisk Forskning
- VINNOVA [2017-03737]
- Swedish government [716321, 721091]
- ALF agreement [716321, 721091]
- Vastra Gotalandsregionen [19-0306]
- Stiftelserna Wilhelm och Martina Lundgrens [2019-2818]
- BioCARE National Strategic Research Program at University of Gothenburg
- Vetenskapsradet [2017-01392, 2016-01530]
- Johan Jansson Foundation for Cancer Research
- Formas [2017-01392] Funding Source: Formas
- Swedish Research Council [2016-01530, 2017-01392] Funding Source: Swedish Research Council
The study demonstrated that cell-free patient-derived scaffolds (PDSs) generated from breast cancer samples, recellularized with cancer cell lines, could be used as a model for testing drug responses in breast cancer. The research showed that MCF7 cancer cells cultured in PDSs exhibited increased resistance against frontline chemotherapy drugs compared to traditional two-dimensional cell cultures, and the gene expression of environmentally adapted cancer cells differed depending on the drug used. This approach offers a novel way to study extracellular matrix influences on cancer drug responses and test novel compounds in in vivo-like microenvironments.
Breast cancer is a heterogeneous disease where the tumor microenvironment, including extracellular components, plays a crucial role in tumor progression, potentially modulating treatment response. Different approaches have been used to develop three-dimensional models able to recapitulate the complexity of the extracellular matrix. Here, we use cell-free patient-derived scaffolds (PDSs) generated from breast cancer samples that were recellularized with cancer cell lines as an in vivo-like culture system for drug testing. We show that PDS cultured MCF7 cancer cells increased their resistance against the front-line chemotherapy drugs 5-fluorouracil, doxorubicin and paclitaxel in comparison to traditional two-dimensional cell cultures. The gene expression of the environmentally adapted cancer cells was modulated in different ways depending on the drug and the concentration used. High doses of doxorubicin reduced cancer stem cell features, whereas 5-fluorouracil increased stemness and decreased the proliferative phenotype. By using PDSs repopulated with other breast cancer cell lines, T-47D and MDA-MB-231, we observed both general and cell line specific drug responses. In summary, PDSs can be used to examine the extracellular matrix influence on cancer drug responses and for testing novel compounds in in vivo-like microenvironments.
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