Article
Plant Sciences
Mingjing Wang, Hailong Zhang, Xiaonan Zhao, Jingwen Zhou, Guochen Qin, Yuqi Liu, Xiaoyue Kou, Zhenjie Zhao, Tao Wu, Jian-Kang Zhu, Xianzhong Feng, Lixin Li
Summary: The gene AtSYP81 in Arabidopsis thaliana plays a critical role in root growth and development by regulating peroxisome- and peroxidase-mediated ROS homeostasis. Knockdown of AtSYP81 resulted in reduced root meristem activity and disruption of root stem cell niche identity. Transcriptome analysis revealed changed expression of class III peroxidases, adding evidence of the regulatory role of AtSYP81 in ROS signaling.
Article
Multidisciplinary Sciences
Sascha Waidmann, Chloe Beziat, Jonathan Ferreira Da Silva Santos, Elena Feraru, Mugurel I. Feraru, Lin Sun, Seinab Noura, Yohann Boutte, Juergen Kleine-Vehn
Summary: This study reveals a mechanism that integrates multiple external cues into auxin-dependent growth programs in Arabidopsis thaliana. This mechanism involves the regulation of PIN-LIKES (PILS) protein abundance and turnover, which affects nuclear auxin signaling and growth rates, contributing to stress-induced growth adaptation in plants.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Zaiqing Wang, Anmin Yu, Fei Li, Wei Xu, Bing Han, Xiaomao Cheng, Aizhong Liu
Summary: This study explored the physiological and molecular mechanisms underlying dwarfism in woody oilseed crop castor bean, revealing divergent cell growth in various tissues and identifying two QTLs associated with plant height. Functional characterization of the Rc5NG4-1 gene encoding an IAA transport protein provided insights into the genetic breeding of castor bean and related crops.
SCIENTIFIC REPORTS
(2021)
Article
Plant Sciences
Xifeng Li, Zhe Wang, Yunting Fu, Xi Cheng, Yan Zhang, Baofang Fan, Cheng Zhu, Zhixiang Chen
Summary: UBAC2 is a multifunctional protein in plant cells that regulates the degradation and accumulation of specific proteins, including COPT and POWDERY MILDEW RESISTANCE 4.
Article
Plant Sciences
Thomas Gate, Lionel Hill, Anthony J. Miller, Dale Sanders
Summary: The study reveals that the AtIAR1 gene in Arabidopsis controls the hydrolysis of stored conjugated auxin through zinc transport, thus affecting root growth. Mutations in AtIAR1 also result in altered auxin metabolism and changes in phenotypes related to auxin activity.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Yuanyuan Zhou, Fenggui Fan, Yongfeng Han, Dongping Lu
Summary: The endoplasmic reticulum is equipped with protein disulfide isomerases, molecular chaperons, and other folding enzymes to ensure proper folding of newly synthesized proteins. Arabidopsis thaliana PDI11 interacts with ER resident lectin chaperones CRT1 and CRT2, with the D domain providing binding sites for this interaction. This implies that Arabidopsis CRT1/2 may specifically recruit AtPDI11 to assist in glycoprotein folding in the ER.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Plant Sciences
Peirui Chen, Yanhua Ge, Liying Chen, Fenglian Yan, Lingling Cai, Hongli Zhao, Deshun Lei, Jinxi Jiang, Meiling Wang, Yi Tao
Summary: This study identified a novel regulator, SAV4, that enhances the membrane clustering and stability of PIN2 through direct protein-protein interactions. SAV4 plays a crucial role in ethylene-induced root hair development and establishing a proper auxin gradient in root tips.
Article
Biochemistry & Molecular Biology
Ludmila Vcelarova, Vladimir Skalicky, Ivo Chamrad, Rene Lenobel, Martin F. Kubes, Ales Pencik, Ondrej Novak
Summary: The endoplasmic reticulum (ER) is a crucial intracellular membrane network involved in protein and lipid synthesis, as well as regulating auxin homeostasis in plants. Isolating ER poses a challenge due to its similarity with other cellular membranes, but an optimized protocol can yield high-quality samples for analyzing auxin metabolites. Analysis of auxin metabolites revealed elevated levels of active auxin form (IAA) in the ER compared to whole plants, and samples prepared using the optim
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Yi Luan, Guangyu Guo, Ying Luan, Yang Yang, Ruixia Yuan
Summary: Pathological cardiac hypertrophy is a precursor to heart failure and requires precise examination of cardiomyocyte subtypes and biological processes for therapeutic approaches. The study analyzed the expression of MAM proteins in cardiac hypertrophy and found a preferential accumulation in cardiomyocytes at the initial stage, followed by a gradual decline. Differentiation trajectory and regulon modules in cardiomyocyte subtypes were identified, along with a cluster of MAM-related genes correlated with diabetic cardiomyopathy. These findings provide potential therapeutic targets for combating cardiac hypertrophy.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Taiyun Wang, Yanhua Zheng, Qi Tang, Songxiao Zhong, Wei Su, Binglian Zheng
Summary: Plant hormone brassinosteroids inhibit miRNA-mediated translational repression by negatively regulating the distribution of AGO1 at the endoplasmic reticulum in Arabidopsis thaliana. This affects the protein levels of miRNA target genes. The findings provide new insights into how miRNA-mediated gene silencing is regulated by plant endogenous hormones.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2021)
Article
Chemistry, Applied
Shane M. Hickey, Ian R. D. Johnson, Elena Dallerba, Mark J. Hackett, Massimiliano Massi, Joanna Lazniewska, Lauren A. Thurgood, Frederick M. Pfeffer, Douglas A. Brooks, Trent D. Ashton
Summary: We introduce a highly fluorescent, solvatochromic, 1,8-naphthalimide probe that enables the simultaneous imaging of lipid droplets and endoplasmic reticulum, with distinguishable emission maxima. By delineating organelles based on their emission profiles, we are able to visualize lipid droplet biogenesis and intercellular trafficking events. Furthermore, this probe demonstrates potential histochemical applications by staining myelin lipids in ex vivo brain tissue.
Article
Developmental Biology
Ke-Zhen Yang, Chao-Ran Zuo, Ya-Jun Leng, Jun-Ling Yue, Hui -Chao Liu, Zhi-Bin Fan, Xue-Yi Xue, Juan Dong, Li-Qun Chen, Jie Le
Summary: In this study, researchers found that the ER quality control system plays an important role in the regulation of the ERECTAf receptor family in stomatal development. By maintaining the protein abundance of the ERECTA receptor and the proper subcellular dynamics of ERL1/ERL2, the ER quality control system ensures the proper execution of receptor functions.
Article
Multidisciplinary Sciences
Yafeng Zheng, Donghui Wang, Sida Ye, Wenqian Chen, Guilan Li, Zhihong Xu, Shunong Bai, Feng Zhao
Summary: This study reveals the crucial role of auxin in guiding GC specification in Arabidopsis anthers. Additionally, it demonstrates that the segregation of GC from somatic cells involves a dynamic feedback circuit among local auxin biosynthesis, transcription of GC determinant protein, and progressive GC specification.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Plant Sciences
Ramin Bahmani, DongGwan Kim, Mahsa Modareszadeh, Seongbin Hwang
Summary: Cadmium enhances root hair elongation in Arabidopsis by increasing reactive oxygen species (ROS) levels and regulating the expression of genes related to root hair elongation.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Multidisciplinary Sciences
Siqi Zhang, Feng-Quan Tan, Ching -Hui Chung, Filip Slavkovic, Ravi Sureshbhai Devani, Christelle Troadec, Fabien Marcel, Halima Morin, Celine Camps, Maria Victoria Gomez Roldan, Moussa Benhamed, Catherine Dogimont, Adnane Boualem, Abdelhafid Bendahmane
Summary: A female-to-male transition mutant was discovered in melon, and the causal gene was identified as the carpel identity gene CRABS CLAW (CRC). The master regulator of sex determination, transcription factor WIP1, recruits the corepressor TOPLESS to suppress CRC expression, leading to male flower development. Impairing the physical interaction between TOPLESS and WIP1 results in CRC expression, carpel determination, and ultimately the development of female flowers.
Article
Plant Sciences
Alexandria F. Harkey, Justin M. Watkins, Amy L. Olex, Kathleen T. DiNapoli, Daniel R. Lewis, Jacquelyn S. Fetrow, Brad M. Binder, Gloria K. Muday
Article
Biotechnology & Applied Microbiology
Muneeza Rai, Maheen Alam, David A. Lightfoot, Priyatansh Gurha, Ahmed J. Afzal
Article
Plant Sciences
Sarbottam Piya, Brad M. Binder, Tarek Hewezi
Article
Plant Sciences
Cidney J. Allen, Randy F. Lacey, Alixandri B. Binder Bickford, C. Payton Beshears, Christopher J. Gilmartin, Brad M. Binder
FRONTIERS IN PLANT SCIENCE
(2019)
Article
Plant Sciences
Yi Chen, Valerie Rofidal, Sonia Hem, Julie Gil, Joanna Nosarzewska, Nathalie Berger, Vincent Demolombe, Mondher Bouzayen, Beenish J. Azhar, Samina N. Shakeel, G. Eric Schaller, Brad M. Binders, Veronique Santoni, Christian Chervin
FRONTIERS IN PLANT SCIENCE
(2019)
Article
Biochemistry & Molecular Biology
Yi Chen, Rasha Althiab Almasaud, Emma Carrie, Guilhem Desbrosses, Brad M. Binder, Christian Chervin
Article
Plant Sciences
Yi Chen, Guojian Hu, Celeste Rodriguez, Meiying Liu, Brad M. Binder, Christian Chervin
HORTICULTURE RESEARCH
(2020)
Review
Biochemistry & Molecular Biology
Brad M. Binder
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Review
Cell Biology
Baptiste Bidon, Samar Kabbara, Vincent Courdavault, Gaelle Glevarec, Audrey Oudin, Francois Hericourt, Sabine Carpin, Lukas Spichal, Brad M. Binder, J. Mark Cock, Nicolas Papon
Article
Multidisciplinary Sciences
Hye Lin Park, Dong Hye Seo, Han Yong Lee, Arkadipta Bakshi, Chanung Park, Yuan-Chi Chien, Joseph J. Kieber, Brad M. Binder, Gyeong Mee Yoon
Summary: Park et al. demonstrate that exposure to ethylene gas leads to the translocation of CTR1 from the endoplasmic reticulum to the nucleus, where it stabilizes the EIN3 transcription factor and enhances the plant's response to ethylene. This discovery sheds light on the mechanism of acclimation of dark-grown seedlings to ethylene and reveals a new link between cellular signaling and physiological responses in the ethylene pathway. Moreover, enhancing nuclear-localized CTR1 in Arabidopsis plants improves their tolerance to drought and salinity stress.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Beenish J. Azhar, Safdar Abbas, Sitwat Aman, Maria Yamburenko, Wei Chen, Lena Mueller, Buket Uzun, David A. Jewell, Jian Dong, Samina N. Shakeel, Georg Groth, Brad M. Binder, Gevorg Grigoryan, Eric Schaller
Summary: The gaseous hormone ethylene is perceived in plants by membrane-bound receptors, with ETR1 from Arabidopsis being the most well-studied receptor. An Asp residue within the ETR1 transmembrane domain is found to play a critical role in ethylene binding. Mutating the Asp to Asn results in a functional receptor with reduced affinity for ethylene, but it still mediates ethylene responses in plants. This study proposes a new structural model for the mechanism of ethylene binding and signal transduction, which shares similarities with a mammalian olfactory receptor.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Plant Sciences
Tao Yao, Jin Zhang, Timothy B. Yates, Him K. Shrestha, Nancy L. Engle, Raphael Ployet, Cai John, Kai Feng, William Patrick Bewg, Margot S. S. Chen, Haiwei Lu, Scott A. Harding, Zhenzhen Qiao, Sara S. Jawdy, Mengjun Shu, Wenya Yuan, Khadijeh Mozaffari, Anne E. Harman-Ware, Renee M. Happs, Larry M. York, Brad M. Binder, Yuko Yoshinaga, Christopher Daum, Timothy J. Tschaplinski, Paul E. Abraham, Chung-Jui Tsai, Kerrie Barry, Anna Lipzen, Jeremy Schmutz, Gerald A. Tuskan, Jin-Gui Chen, Wellington Muchero
Summary: This study identified PtrXB38 as an expression quantitative trait loci (eQTL) hotspot, with predicted target genes involved in plant hormone responses and root development. Overexpression of PtrXB38 led to increased callusing and the formation of stem-born and base-born adventitious roots in Populus. Omics studies revealed the involvement of genes and proteins controlling auxin transport and signaling in PtrXB38-mediated root formation. Protein-protein interaction assays indicated that PtrXB38 interacts with components of the endocytosis pathway.
Article
Multidisciplinary Sciences
Eric Brenya, Esha Dutta, Brittani Herron, Lauren H. Walden, Daniel M. Roberts, Brad M. Binder
Summary: Enhancing crop yields is a major challenge, but pretreating Arabidopsis seedlings with ethylene can lead to long-term growth enhancement and increased stress tolerance. This is attributed to ethylene-induced photosynthetic-derived sugars, which increase carbon assimilation and levels of glucose, sucrose/trehalose, and starch. Metabolic and transcriptomic analyses showed increased metabolic processes and gene transcripts related to cell division, photosynthesis, and carbohydrate metabolism. This priming of metabolism causes increases in photosynthesis and carbohydrate utilization, ultimately promoting growth.
Review
Chemistry, Physical
T. Scott Carlew, Cidney J. Allen, Brad M. Binder
Article
Plant Sciences
Randy F. Lacey, Cidney J. Allen, Arkadipta Bakshi, Brad M. Binder