Article
Biology
Angelika V. Timofeeva, Ivan S. Fedorov, Maria A. Shamina, Vitaliy V. Chagovets, Nataliya P. Makarova, Elena A. Kalinina, Tatiana A. Nazarenko, Gennady T. Sukhikh
Summary: The study utilized small noncoding RNAs (sncRNAs) to assess embryonic implantation potential, developing two models with 100% sensitivity and specificity for accurate prediction. The results indicated the involvement of sncRNA target gene protein products in regulating various cellular processes critical for blastomere fate determination.
Article
Multidisciplinary Sciences
Dongyang Wang, Zhenghao Zhao, Xia Xue, Juanzi Shi, Wenhao Shi
Summary: This study investigated the potential of glycan profile in spent blastocyst culture medium as a biomarker for predicting implantation outcome. It was found that the binding signals of 10 lectins were different between successful and failed implantation samples, with increased glycan binding to lectins NPA, UEA-I, MAL-I, LCA and GNA, and decreased binding to lectins DBA and BPL in successful implantation. The glycan profile in spent culture medium showed no significant differences in embryos with different morphological grades except for the binding of UEA-I between Poor and Medium blastocysts. Therefore, detection of glycan profile in spent culture medium may provide a non-invasive assessment assay of embryo viability and contribute to understanding molecular mechanisms in embryo implantation.
Article
Biochemistry & Molecular Biology
Silvia Toporcerova, Ivana Spakova, Katarina Soltys, Zuzana Klepcova, Marek Kl'oc, Julia Bohosova, Karolina Trachtova, Lucia Peterova, Helena Mickova, Peter Urdzik, Maria Marekova, Ondrej Slaby, Miroslava Rabajdova
Summary: The increased interest in assisted reproduction through in vitro fertilization (IVF) has created a need for biomarkers that can highly predict the success of pregnancy. Researchers have found that analyzing short non-coding RNA (sncRNA) molecules secreted during the IVF process using artificial intelligence can enhance our understanding of communication between embryonic cells and the endometrium, leading to increased success rates.
Article
Veterinary Sciences
Paul Del Rio, Pavneesh Madan
Summary: Distinct miRNA populations have been detected in the spent media of in-vitro culture systems throughout the pre-implantation period, with a total of 111 miRNAs identified. These miRNAs may impact early embryo development by regulating genes involved in cellular processes, metabolic processes, and biological regulation.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Fisheries
Ye Ge, Qiucheng Yao, Yingying Wang, Qing Wang, Weiwei Zeng
Summary: The study utilized miRNA library construction and microarray sequencing to screen out miRNAs with significant differences between the GCRV infection group and the control group. The results showed the importance of related miRNAs and mRNA target genes in immune effect processes, regulation of immune effect processes, cell death, regulation of leucocyte-mediated cytotoxicity, potentially involving deregulation of the PI3K/AKT, Toll-like, and RIG-I signaling pathways.
AQUACULTURE RESEARCH
(2021)
Review
Chemistry, Multidisciplinary
Iza Sazanita Isa, Umi Kalsom Yusof, Murizah Mohd Zain
Summary: The development of intelligence-based methods and application systems has expanded the use of quality blastocyst selection in IVF. In this study, the state-of-the-art in ART is established using an automated deep learning approach, and the scoping review introduces a novel notion in automated blastocyst grading utilizing deep learning applications. These automated methods can match or even outperform skilled embryologists in certain deep learning tasks.
APPLIED SCIENCES-BASEL
(2023)
Article
Oncology
Shanshan Wang, Lei Chen, Yingchun Zhu, Weihua Jiang
Summary: The study identified potential biomarkers for embryo quality during IVF cycles using RNA sequencing, and found three miRNAs that may serve as biomarkers for embryo quality during IVF cycles.
ANNALS OF TRANSLATIONAL MEDICINE
(2021)
Article
Medicine, Research & Experimental
Yidong Chen, Yuan Gao, Jialin Jia, Liang Chang, Ping Liu, Jie Qiao, Fuchou Tang, Lu Wen, Jin Huang
Summary: The discovery of embryonic cell-free DNA in spent embryo culture media has provided hope for noninvasive preimplantation genetic testing. However, the cellular origins of this DNA are not well understood, and methods for detecting maternal DNA contamination are limited. This study used whole-genome DNA methylation sequencing to identify the sources of cell-free DNA in spent embryo culture media, providing insights into noninvasive preimplantation genetic testing.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Biology
Setu Chakraborty, Nardos T. Woldemariam, Tina Visnovska, Matthew L. Rise, Danny Boyce, Javier Santander, Rune Andreassen
Summary: This study characterized the miRNA repertoire of lumpfish using small RNA sequencing and miRDeep analysis. A total of 443 unique mature miRNAs were identified, with enrichment of specific miRNAs in particular organs and developmental stages. These miRNAs may play a role in regulating organ and developmental stage-specific functions in lumpfish.
Article
Endocrinology & Metabolism
Zhanhui Ou, Yu Deng, Yunhao Liang, Zhiheng Chen, Ling Sun
Summary: This study compared the detection rates of beta-thalassemia using spent culture medium and spent culture medium containing blastocoelic fluid. The results showed that using spent culture medium containing blastocoelic fluid had a higher detection rate, and the DNA concentration in the spent culture medium was associated with fragmentation.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jianxiang Zhang, Cheng Xu, Kangwei Liu, Yaoqinq Li, Mengna Wang, Lian Tao, Hengxiu Yu, Chao Zhang
Summary: Plants may suffer DNA damage under extreme environmental conditions, leading to chromosomal abnormalities or cell death if not repaired rapidly and accurately. Organisms have evolved various DNA repair mechanisms, including the regulation of DNA damage repair by miRNAs targeting gene transcription. Specific miRNAs are found to be related to DNA repair processes, providing valuable insights for further functional studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Veterinary Sciences
Janne G. Lyngby, Marta Godia, Louise Brogaard, Annemarie T. Kristensen, Merete Fredholm, Ellen Skancke, Joanna Morris, Nana Dupont, Silke Salavati Schmitz, David Argyle, Armand Sanchez, Charlotte R. Bjornvad, Susanna Cirera, Lise N. Nielsen
Summary: This study found that specific microRNAs in feces and serum of dogs can be used as noninvasive biomarkers to differentiate gastrointestinal cancer from chronic inflammatory enteropathy. MiR-451 and miR-223 showed the best diagnostic performance, and combining them slightly improved the diagnostic effectiveness.
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2022)
Article
Genetics & Heredity
Zhiyong Liu, Qiangwei Wang, Nana Wang, Yu Zang, Riga Wu, Hongyu Sun
Summary: This study characterized the small RNA profiles in common forensic stains and identified relative specific markers and absolute specific markers that can be used for body fluid/tissue identification in forensic scenarios.
Article
Obstetrics & Gynecology
Iris G. Insogna, Andrea Lanes, Elizabeth S. Ginsburg, Catherine Racowsky
Summary: The refreshment of medium on day 6 did not increase the proportion of usable embryos on day 7 overall, with an adverse effect seen in women aged 40 and above. Extending the culture of embryos to day 7 resulted in additional blastocysts available for clinical use.
HUMAN REPRODUCTION
(2021)
Article
Obstetrics & Gynecology
Alexander Freis, Sabine Roesner, Anne Marshall, Julia Rehnitz, Kyra von Horn, Edison Capp, Jens Erik Dietrich, Thomas Strowitzki, Ariane Germeyer
Summary: This study collected spent culture media from blastocysts transferred at day 5, analyzing differentially expressed proteins to identify potential biomarkers for non-invasive embryo assessment. The media from implanted blastocysts showed significantly higher expression of certain proteins compared to media from blastocysts without implantation, indicating possible biomarkers for future use in non-invasive embryo assessment.
REPRODUCTIVE SCIENCES
(2021)
Correction
Developmental Biology
Angelika V. Timofeeva, Vladyslava A. Gusar, Nataliya E. Kan, Kseniya N. Prozorovskaya, Anna O. Karapetyan, Oleg R. Bayev, Vitaliy V. Chagovets, Sergei F. Kliver, Daria Yu Iakovishina, Vladimir E. Frankevich, Gennadiy T. Sukhikh
Article
Developmental Biology
Angelika V. Timofeeva, Vladyslava A. Gusar, Nataliya E. Kan, Kseniya N. Prozorovskaya, Anna O. Karapetyan, Oleg R. Bayev, Vitaliy V. Chagovets, Sergei F. Kliver, Daria Yu. Iakovishina, Vladimir E. Frankevich, Gennadiy T. Sukhikh
Article
Biochemistry & Molecular Biology
Angelika V. Timofeeva, Vitaliy V. Chagovets, Yulia S. Drapkina, Nataliya P. Makarova, Elena A. Kalinina, Gennady T. Sukhikh
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Medicine, General & Internal
Vladislava Gusar, Angelika Timofeeva, Vitaliy Chagovets, Nataliya Kan, Oksana Vasilchenko, Kseniya Prozorovskaya, Tatyana Ivanets, Gennadiy Sukhikh
JOURNAL OF CLINICAL MEDICINE
(2020)
Article
Medicine, General & Internal
Angelika Timofeeva, Ivan S. Fedorov, Alexander G. Brzhozovskiy, Anna E. Bugrova, Vitaliy V. Chagovets, Maria V. Volochaeva, Natalia L. Starodubtseva, Vladimir E. Frankevich, Evgeny N. Nikolaev, Roman G. Shmakov, Gennady T. Sukhikh
Summary: Despite differing clinical manifestations, the pathogenesis of PE and IUGR both involve dysregulation of cytotrophoblast cells, with molecular differences primarily found in the placental bed samples. Unique miRNA and target gene expression changes in the placental bed samples were associated with abnormality in the hemostatic and vascular systems, as well as inflammation at the fetal-maternal interface, indicating potential links to the development of both conditions.
Article
Biology
Angelika Timofeeva, Ivan S. Fedorov, Mariya M. Pirogova, Oksana N. Vasilchenko, Vitaliy V. Chagovets, Larisa S. Ezhova, Tatiana M. Zabelina, Roman G. Shmakov, Gennadiy T. Sukhikh
Summary: This study aimed to optimize the diagnosis of abnormally invasive placenta (AIP) using a combination of microRNA and gene-target protein levels in blood plasma samples from pregnant women. Significant increases in certain microRNAs were found in women with AIP, while opposite changes in gene-target protein/miRNA pairs were observed. Logistic regression models using specific microRNAs and protein levels showed high sensitivity and specificity in diagnosing AIP cases of varying severity.
Article
Biology
Vladislava Gusar, Angelika Timofeeva, Vitaliy Chagovets, Nataliya Kan, Mikhail Vysokikh, Maria Marey, Anna Karapetyan, Oleg Baev, Gennadiy Sukhikh
Summary: The expression of hypoxamiRs in exosomes is significantly altered in early-onset preeclampsia (PE), and it correlates with blood parameters and the expression of other proteins in the placenta, suggesting the potential of exosomal hypoxamiRs as diagnostic markers for early-onset PE.
Article
Biology
Angelika V. Timofeeva, Ivan S. Fedorov, Maria A. Shamina, Vitaliy V. Chagovets, Nataliya P. Makarova, Elena A. Kalinina, Tatiana A. Nazarenko, Gennady T. Sukhikh
Summary: The study utilized small noncoding RNAs (sncRNAs) to assess embryonic implantation potential, developing two models with 100% sensitivity and specificity for accurate prediction. The results indicated the involvement of sncRNA target gene protein products in regulating various cellular processes critical for blastomere fate determination.
Article
Biochemistry & Molecular Biology
Vladislava A. Gusar, Angelika V. Timofeeva, Vitaliy V. Chagovets, Mikhail Yu. Vysokikh, Nataliya E. E. Kan, Ludmila A. Manukhova, Maria V. Marey, Gennadiy T. Sukhikh
Summary: Redox disbalance in placental cells leads to the hyperproduction of reactive oxygen species (ROS), causing dysregulation of maternal immune tolerance, pro-inflammatory reactions, and perinatal complications. Microvesicles containing miRNAs are involved in ROS balance. This study evaluates markers of redox balance and miRNA expression in newborns with FGR, IP, NS, and RDS. Significant differences in MDA and antioxidant enzyme levels were found in newborns with FGR. miR-127-3p expression increased in newborns with IP, NS, and RDS, while miR-25-3p expression increased only in newborns with NS. Transcription factors involved in OS, immune response, and inflammation were identified as targets of studied miRNAs. miR-127-3p was associated with pro-inflammatory reactions, while miR-25-3p was associated with inhibition of macrophage migration and activation of antioxidant enzymes.
Article
Biology
Angelika V. V. Timofeeva, Aleksandra V. V. Asaturova, Maya V. V. Sannikova, Grigory N. N. Khabas, Vitaliy V. V. Chagovets, Ivan S. S. Fedorov, Vladimir E. E. Frankevich, Gennady T. T. Sukhikh
Summary: Recent studies have focused on using RNA quantification to predict disease prognosis in epithelial ovarian cancer (EOC). In this study, the researchers analyzed the expression levels of certain RNAs and proteins in high-grade serous ovarian carcinoma (HGSOC) and identified different subtypes with significant differences in treatment response and prognosis. This research provides an effective method for predicting treatment response and prognosis in patients with EOC.