期刊
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
卷 2021, 期 2, 页码 253-265出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/ejoc.202001267
关键词
BODIPY; Cell-staining; Mechanochemistry; Multicomponent reaction; Phagocytosis
资金
- Consejo Nacional de Ciencia y Tecnologia (CONACYT, Mexico) [A1-S-44097]
- Spanish Ministerio de Economia y Competitividad [MAT2017-83856-C3-3-P]
- Gobierno Vasco [IT912-16]
- CONACYT [70766, 253623, 123732, 2013-205744, 2019-300286, FC-2015-02-834]
- DAIP grant 2020
- PRODEP [511-6/17-9392]
- US Army [W911NF1810428]
- U.S. Department of Defense (DOD) [W911NF1810428] Funding Source: U.S. Department of Defense (DOD)
Mechanochemistry is used to prepare valuable dyes, with the molecular structure and fluorescence interaction playing a key role in fluorescence response, demonstrating high brightness in cellular media. The novel dyes efficiently stain PBMCs and Candida albicans cells, and can monitor different stages of phagocytosis.
Mechanochemistry, that is, the use of mechanical energy produced by milling or grinding to perform chemical transformations, was used as a green strategy using a minimal amount of solvent for the preparation of highly valuable formyl functionalized BODIPY dyes. The electronic properties of the meso-aryl group play a key role in the fluorescence response of the adducts resulting from the Ugi reaction. Such an understanding of the molecular structure-fluorescence interplay explains the brightness of these dyes in the cellular media monitored by fluorescence imaging. In the present report, we provide flow cytometry and fluorescence microscopy evidence that the novel dyes efficiently stain peripheral blood mononuclear cells (PBMCs) and Candida albicans cells without disturbing cell integrity. Moreover, it is demonstrated that by staining C. albicans cells, we can monitor the early and late steps of phagocytosis, using human monocyte-derived macrophages. The dye series presented herein represent a novel application for formyl functionalized BODIPYs, expanding their applications in monitoring biological processes.
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