Article
Microbiology
Babak Najafpour, Patricia I. S. Pinto, Katerina A. Moutou, Adelino V. M. Canario, Deborah M. Power
Summary: The study revealed that bacterial communities in fish eggs and water differ in abundance but share similar functions. Factors such as sample type, species, and collection site drive microbial community structures.
Article
Multidisciplinary Sciences
Stefanos Fragkoulis, Dimosthenis Kerasovitis, Costas Batargias, George Koumoundouros
Summary: Juvenile fish body shape experiences rapid change followed by a stable period before becoming adults. The correlation between juvenile and adult body shapes ranges from 0.22 to 0.76. The heritability estimates of the final phenotype ranged from 0.370 to 0.511, with a heritability of 0.173 for the phenotypic integration rate. The study demonstrates a substantial additive genetic component in the variance of ontogenetic trajectories.
SCIENTIFIC REPORTS
(2021)
Article
Fisheries
Jose Carlos A. Campos-Sanchez, Francisco A. Guardiola, Mara Angeles Esteban
Summary: This study investigated the effects of cantharidin on the inflammatory response in gilthead seabream head-kidney leucocytes (HKLs). The results showed that cantharidin induced apoptosis in HKLs, decreased respiratory burst and phagocytic activities, and increased peroxidase activity. Furthermore, cantharidin also affected the expression of different genes in HKLs.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Environmental Sciences
Cassandre Aimon, Christophe Lebigre, Nicolas Le Bayon, Stephane Le Floch, Guy Claireaux
Summary: This study demonstrates that juvenile sea bass exposed to oil spills exhibit transient behavioral impairments, with a dose-response relationship observed. Recovery capacities were seen within the first 10 days post-exposure, although fish may require more time for full recovery. The alterations in behavior may be attributed to neurosensory or physiological defects caused by oil exposure, leading to sedative behaviors resembling anesthesia.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Biochemistry & Molecular Biology
Andrea Miccoli, Laura Guerra, Valeria Pianese, Paolo Roberto Saraceni, Francesco Buonocore, Anna Rita Taddei, Ana Couto, Tania De Wolf, Anna Maria Fausto, Giuseppe Scapigliati, Simona Picchietti
Summary: Our study on European sea bass reveals that TRγ cells develop earlier than TRβ cells, with the thymus showing the highest expression of TRγ. TRγ cells are mainly distributed in peripheral blood and lymphoid organs, with abundant expression in gut mucosa. Microalgae-based diet can significantly modulate the abundance of TRγ cells in the posterior intestine, potentially involved in nutritional immunity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Joana P. Firmino, Laura Fernandez-Alacid, Eva Vallejos-Vidal, Ricardo Salomon, Ignasi Sanahuja, Lluis Tort, Antoni Ibarz, Felipe E. Reyes-Lopez, Enric Gisbert
Summary: Dietary supplementation of garlic essential oil, carvacrol, and thymol in gilthead seabream juveniles modulated skin transcriptional immune response, bacterial growth capacity in skin mucus, and overall health condition. The supplementation promoted innate immunity and mucus protective capacity, reducing susceptibility to pathogenic bacteria colonization.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Fisheries
Maria M. Camara-Ruiz, Jose Maria Garcia-Beltran, Isabel M. Cerezo, M. Carmen Balebona, Miguel Angel Morinigo, Maria Angeles Esteban
Summary: Fish stress is a major concern in aquaculture industry. This study found that air exposure stress caused changes in skin mucosal immunity and microbial composition in gilthead seabream, which may be beneficial for the fish to adapt to stress.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Review
Fisheries
Nunziatina Porcino, Lucrezia Genovese
Summary: This review provides a global overview of alternatives to fish meal and fish oil in aquaculture feeds for gilthead sea bream, with a focus on gut health. The collected information may serve as a tool for fine-tuning new diets for gilthead sea bream.
AQUACULTURE RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
Lourenco Ramos-Pinto, Marina Machado, Josep Calduch-Giner, Jaume Perez-Sanchez, Jorge Dias, Luis E. C. Conceicao, Tome S. Silva, Benjamin Costas
Summary: The study aimed to evaluate the effects of histidine, taurine, and threonine supplementation on the immune status of gilthead seabream. The results showed that dietary supplementation with these amino acids had mild immune-modulation effects on gilthead seabream, particularly noteworthy for further study under disease challenge conditions.
Article
Biology
Alexandre Mignucci, Jerome Bourjea, Fabien Forget, Hossein Allal, Gilbert Dutto, Eric Gasset, David J. McKenzie
Summary: The study found that cardiac responses of gilthead seabream to hypoxia and warming differed between swimming freely in a tank and being confined to individual respirometers, with spontaneous activity contributing to heart rate differences. Additionally, there was a significant linear relationship between heart rate and oxygen uptake rates, suggesting that respirometry techniques may bias metabolic trait estimates in some fishes.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Denise Schrama, Claudia Raposo de Magalhaes, Marco Cerqueira, Raquel Carrilho, Dominique Revets, Annette Kuehn, Sofia Engrola, Pedro M. Rodrigues
Summary: This study focused on the characterization of parvalbumin in gilthead seabream and European seabass. It found that parvalbumins from both species are susceptible to digestion and processing techniques. These findings contribute to further research on fish allergenicity.
Article
Biochemistry & Molecular Biology
Miguel Angel Garcia-Alvarez, Marta Arizcun, Elena Chaves-Pozo, Alberto Cuesta
Summary: The study found that gilthead seabream larvae are susceptible to both RGNNV/SJNNV and SJNNV/RGNNV reassortants, with the highest impact from the RGNNV/SJNNV reassortant on 37-day post-hatching larvae. Viral replication occurs at both 37 and 86 dph, but the recovery of infective particles was only confirmed in 37 dph larvae.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Chara Kourkouta, Andreas Tsipourlianos, Deborah M. Power, Katerina A. Moutou, George Koumoundouros
Summary: Skeletal abnormalities are important indicators in finfish hatcheries, and their occurrence is related to rearing conditions. Gill-cover folding and pugheadedness are the most common abnormalities. A new abnormality, ray-resorption syndrome, was also identified. Different rearing variables were found to be important predictors for different abnormalities and survival rate. Genetic analysis showed diversity and distances among hatcheries, suggesting the role of company-specific management in variability of KPIs.
SCIENTIFIC REPORTS
(2022)
Article
Fisheries
Rita Teodosio, Claudia Aragao, Luis E. C. Conceicao, Jorge Dias, Sofia Engrola
Summary: The P/E ratio has a significant impact on protein utilization and metabolism in gilthead seabream juveniles. Lower P/E ratios result in increased amino acid uptake efficiency, while higher P/E ratios enhance lysine utilization and promote growth.
AQUACULTURE NUTRITION
(2022)
Article
Fisheries
Jose Carlos Campos-Sanchez, Francisco A. Guardiola, Maria Angeles Esteban
Summary: This study investigated the effects of lambda-carrageenin on gilthead seabream head-kidney leucocytes (HKLs). The results showed that lambda-carrageenin had no negative effects on cell viability but increased respiratory burst activity and phagocytic ability of HKLs. It also affected the expression of inflammation-related genes.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Laura Cervera, Carmen Gonzalez-Fernandez, Daniela Cano, M. Angeles Esteban, Luis Mercado, Elena Chaves-Pozo, Alberto Cuesta
Summary: Antimicrobial peptides (AMPs) are potent immune system components that can kill pathogens and modulate the immune response. This study found that AMPs in European sea bass can enhance immune response and antibacterial activity but do not improve resistance to nodavirus (NNV) disease.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Yulema Valero, Luis Mercado, Marta Arizcun, Alberto Cuesta, Elena Chaves-Pozo
Summary: Immunity plays a crucial role in the development of fish embryos, and maternal transfer of immunity positively affects the innate immune system development of their progeny. In this study, priming broodstock female fish before spawning season enhances the innate immune activities and upregulates the expression of antimicrobial peptides in their offspring. Furthermore, evidence of maternal transfer of immune proteins related to antimicrobial responses is provided.
Article
Agriculture, Dairy & Animal Science
Maria Dolores Ayala, Noemi Balsalobre, Elena Chaves-Pozo, Maria Isabel Saez, Alba Galafat, Francisco Javier Alarcon, Tomas Francisco Martinez, Marta Arizcun
Summary: This study investigated the long-term effects of microalgae-enriched diets on the growth of juvenile seabream. Results showed that the inclusion of microalgae in the diet influenced body and muscle growth, with the hydrolyzed diet and higher inclusion levels having a more significant effect. The findings suggest that incorporating microalgae in the juvenile diet can improve subsequent growth, which is beneficial for fish farmers.
Article
Fisheries
Miguel Angel Garcia-Alvarez, Carmen Gonzalez-Fernandez, Maria Angeles Esteban, Alberto Cuesta
Summary: In this study, the cytotoxic and regulatory T cell molecule CRTAM and its ligand CADM1 were identified in European seabass and gilthead seabream. The transcriptional levels of these genes were evaluated and showed constitutive expression, with higher expression in immune tissues. Nodavirus infection induced up-regulation of CRTAM and CADM1, particularly in seabream, which may explain their resistance to nodavirus disease.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
M. I. Saez, A. Galafat, M. D. Suarez, E. Chaves-Pozo, M. Arizcun, M. D. Ayala, F. J. Alarcon, T. F. Martinez
Summary: Numerous studies have shown that incorporating microalgae into fish feed has positive effects on various physiological aspects of aquacultured fish. This study examines the effects of microalgae-enriched diets on the quality parameters of gilthead seabream fillets. The results suggest that these diets improve fatty acid profile, reduce microbial counts, enhance lipid oxidative status, and enhance textural and skin color attributes of the fillets. However, the enzymatic pre-treatment of the microalgal biomass did not show a consistent trend in its effects on quality parameters.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Review
Chemistry, Medicinal
Jose Maria Garcia-Beltran, Marta Arizcun, Elena Chaves-Pozo
Summary: Aquaculture production is increasing, but infectious diseases pose a threat. Antimicrobial peptides (AMPs) from photosynthetic marine organisms have potential as alternatives to antibiotics in aquaculture due to their defense mechanisms and additional activities. This study reviews the knowledge about AMPs from photosynthetic marine organisms and evaluates their suitability for aquaculture.
Review
Fisheries
Yulema Valero, Alberto Cuesta
Summary: Aquaculture is responsible for over half of the fish consumed by humans worldwide, but infectious diseases caused by viruses are a major problem. The coexistence of different virus strains in the same area allows for natural reassortment, which is a recombination process where different viruses exchange their genetic material. This has been facilitated by the transportation of fish eggs and larvae between facilities globally.
REVIEWS IN AQUACULTURE
(2023)
Article
Fisheries
Salma Guebebia, Cristobal Espinosa-Ruiz, Lazhar Zourgui, Alberto Cuesta, Mehrez Romdhane, M. Angeles Esteban
Summary: The study examined the immunostimulant, cytotoxic, bactericidal, and antioxidant activities of ethanolic extracts from different parts of okra. The extracts showed significant content of phenols and flavonoids. They had notable effects on leukocyte activities in European sea bass and demonstrated cytotoxic effects on cell lines. Furthermore, they exhibited bactericidal activity against fish pathogenic bacteria and antioxidant properties. The results suggest the potential use of okra extracts as an alternative to chemical compounds in aquaculture.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Editorial Material
Biochemistry & Molecular Biology
Elena Chaves-Pozo, Alberto Cuesta
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Fisheries
Javier Mayor, Alberto Cuesta, Cristobal Espinosa-Ruiz, M. Angeles Esteban
Summary: This study investigated the effects of Astaxanthin (AX) on various fish pathogenic bacteria and fish cell lines. The results showed that AX had different effects on different bacteria and cell lines, providing valuable insights into the mechanism of action of AX in aquaculture.
AQUACULTURE REPORTS
(2023)
Article
Fisheries
Maria Dolores Ayala, Elena Chaves-Pozo, Maria Isabel Saez, Alba Galafat, Francisco Javier Alarcon, Tomas Francisco Martinez, Marta Arizcun
Summary: This experiment investigated the effect of Nannochloropsis gaditana on muscle growth in gilthead seabream. The results showed no significant differences in body parameters and white muscle transverse area (WM) among the groups, but muscle cellularity did show significant differences. The raw feed groups had higher fibrillar density and hyperplasia values, which could improve the final quality of the fish.
Article
Fisheries
Ratiporn Thongsoi, Siriluk Maskaew, Panumas Puechpon, Benjaporn Noppradit, Napassawan Inaek, Prapaporn Utarabhand, Phanthipha Runsaeng
Summary: In this study, a PPAE gene called FmPPAE1 was cloned and analyzed in banana shrimp. FmPPAE1 plays a vital role in regulating the proPO system, exhibits antibacterial properties, and contributes to the suppression of bacterial proliferation.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Jishuang Tan, Paizelaiti Sahaer, Huan Li, Wei Han, Hongyan Sun
Summary: Circular RNAs (circRNA) are involved in gene expression and affect cellular processes such as transcription, splicing, and translation by interacting with microRNA and RNA binding proteins. A study identified circDNAJB6 as a circular RNA abundant in chicken macrophages after bacterial infection, and found that circDNAJB6 exacerbates cellular injury and interacts with multiple miRNAs and target genes, potentially impacting autophagy and MAPK signaling pathways.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Rodrigo Guabiraba, Damaris Ribeiro Rodrigues, Paul T. Manna, Melanie Chollot, Vincent Saint-Martin, Sascha Trapp, Marisa Oliveira, Clare E. Bryant, Brian J. Ferguson
Summary: The innate immune response relies on the ability of host cells to detect and respond to microbial nucleic acids. Toll-like receptors (TLRs) play a crucial role in this process by distinguishing self from non-self. This study focused on TLR21, an avian TLR that recognizes bacterial DNA motifs. The findings suggest that avian TLR21 shares similar activation mechanisms to mammalian TLR9, highlighting the conservation of nucleic acid sensing mechanisms across species.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Safieh Zeinali, Kate Sutton, Lonneke Vervelde
Summary: Chickens have a unique immune structure characterized by the absence of draining lymph nodes and the presence of well-developed mucosa-associated lymphoid tissue. The structure and development of chicken lymphoid tissues in the intestine, particularly Peyer's patches, have been poorly studied. However, the use of CSF1R-eGFP reporter transgenic chickens has allowed for the visualization and investigation of the development and distribution of these tissues. This research provides insights that could benefit mucosal vaccination strategies and the development of targeted vaccine delivery systems.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Retraction
Fisheries
G. -g. Li, Z. -z. Guo, X. -f. Ma, N. Cao, S. -n. Geng, Y. -q. Zheng, M. -j. Meng, H. -h. Lin, G. Han, G. -j. Du
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Jinyue Yang, Peng Zhou, Wanrong Wu, Qingxiang Zhang, Dong Chen, Rui Luo
Summary: This study cloned and characterized the full-length sequence of duck MRE11 (duMRE11) for the first time, and found that this protein plays an important role in the innate immune response of ducks.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Mengjiao Guo, Jiaqi Zhang, Mingtao Li, Xiaorong Zhang, Yantao Wu
Summary: rNLRC3 negatively regulates the NF -KB signaling pathway induced by P. multocida in rabbits by inhibiting the activation of NF -KB, reducing the expression of inflammatory cytokines, and interacting with rTRAF4 and rTRAF6. The NACHT-LRR domain is the functional domain of rNLRC3. These findings provide insights into the important role of rNLRC3 in combating P. multocida infection.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Yinan Li, Xingye Lian, Wenwen Yang, Jinyuan Leng, Jiejie Sun, Yu Liu, Siqi Fan, Lingling Wang, Linsheng Song
Summary: The DM9-containing protein CgDM9CP-7 plays an important role in the innate immune response of molluscs. It is constitutively expressed in various tissues and is up-regulated in haemocytes after stimulation by Vibrio splendidus and lipopolysaccharide (LPS). CgDM9CP-7 binds to various pathogens and exhibits antimicrobial activity, while also regulating the expression of CgMyD88 and CgIL-17.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
Article
Fisheries
Xiuwen Bao, Haoran Song, Liying He, Yong Li, Shuqi Niu, Jinlin Guo
Summary: This study focuses on the interaction between Hepialus xiaojinensis larvae and Ophiocordyceps sinensis during the infection process. The results show that the infected third instar larvae enhance antioxidant defense ability, while the infected fourth instar larvae reduce this ability and allocate more energy to growth and development.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2024)
