期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 65, 期 -, 页码 340-351出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2016.07.020
关键词
Germfree animals; Ileal Peyers patches; Immunoglobulin A; Blind ileal loops
资金
- Czech Science Foundation Grant [15-02274S]
- Institutional Research Concept of the Institute of Microbiology of the Czech Academy of Sciences [v.v.i. RVO 61388971]
- NSF-Integrative Organismal Biology Grant [15203800]
The Heal Peyers patches (IPP) of newborn germfree (GF) piglets were isolated into blind loops and the piglets colonized with a defined probiotic microflora. After 5 weeks, IgA levels in the intestinal lavage (IL) of loop piglets remained at GF levels and IgM comprised similar to 70% while in controls, IgA levels were elevated 5-fold and comprised similar to 70% of total Igs. Loop piglets also had. reduced serum IgA levels suggesting the source of serum IgA had been interrupted. The isotype profile for loop contents was intermediate between that in the IL of GF and probiotic controls. Surprisingly, colonization alone did not result in repertoire diversification in the IPP. Rather, colonization promoted pronounced proliferation of fully switched IgA(+)IgM(-) B cells in the IPP that supply early, non-diversified natural SIgA antibodies to the gut lumen and a primary IgA response in serum. (C) 2016 Elsevier Ltd. All rights reserved.
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