期刊
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
卷 63, 期 -, 页码 163-170出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2016.06.002
关键词
Extracellular superoxide dismutase; RGD motif; Crassostrea gigas; Inhibiting factor; Immune recognition; Immune priming
资金
- High Technology Project (863 Program) from the Chinese Ministry of Science and Technology [2014AA093501]
- National Natural Science Foundation of China [31530069, 31402337]
- earmarked fund from Modern Agro-industry Technology Research System [CARS-48]
- National & Local Joint Engineering Laboratory of Ecological Mariculture
- Taishan Scholar Program of Shandong, China
Extracellular superoxide dismutase (EcSOD) is a copper-containing glycoprotein playing an important role in antioxidant defense of living cells exposed to oxidative stress, and also participating in microorganism internalization and cell adhesion in invertebrates. EcSOD from oyster (designated CgEcSOD) had been previously reported to bind lipopolysaccharides (LPS) and act as a bridge molecule in Vibrio splendidus internalization. Its mRNA expression pattern, PAMP binding spectrum and microorganism binding capability were examined in the present study. The mRNA expression of CgEcSOD in hemocytes was significantly up-regulated at the initial phase and decreased sharply at 48 h post V. splendidus stimulation. The recombinant CgEcSOD protein (rCgEcSOD) could bind LPS, PGN and poly (I:C), as well as various microorganisms including Micrococcus luteus, Staphylococcus aureus, Escherichia coli, Vibrio anguillarum, V splendidus, Pastoris pastoris and Yarrowia lipolytica at the presence of divalent metal ions Cu2+. After the secondary V. splendidus stimulation, the mRNA and protein of CgEcSOD were both down regulated significantly. The results collectively indicated that CgEcSOD could not only function in the immune recognition, but also might contribute to the immune priming of oyster by inhibiting the foreign microbe invasion through a specific down-regulation. (C) 2016 Elsevier Ltd. All rights reserved.
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