Review
Biochemistry & Molecular Biology
Wei Wang, Hanxian Xiong, Kaiting Sun, Bo Zhang, Meng-Xiang Sun
Summary: This study reviews the communication among the endosperm, embryo, and seed coat during seed development and highlights recent advances in this field.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Plant Sciences
Wenxuan Zou, Gang Li, Liufang Jian, Jie Qian, Yantong Liu, Jie Zhao
Summary: The study highlights the importance of AtSMC6A and AtSMC6B in plant development, showing that they play critical roles in seed and gametophyte development through maintaining chromosome stability in Arabidopsis.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Review
Biochemistry & Molecular Biology
Ji Min Shin, Ling Yuan, Tomokazu Kawashima
Summary: Seed development in flowering plants is a complex process regulated by genetically distinct tissues. Live-cell imaging techniques have allowed us to explore the dynamic events involved in seed development.
Article
Plant Sciences
Chengcheng Li, Fan Hu, Hongyu Chen, Jie Zhao
Summary: The study characterized the early embryo and endosperm development in the naa15 mutant compared to the wild type of Arabidopsis, showing abnormal embryo differentiation and incomplete endosperm cellularization. Transcriptome analyses revealed differentially expressed genes (DEGs) involved in lipid metabolic processes, cell wall biogenesis, hormone signaling pathways, and transcriptional regulation. Immunofluorescent assays also showed differences in the features of cell walls, providing important insights into the regulatory mechanisms of endosperm cellularization and embryonic differentiation.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Naoto Sano, Imen Lounifi, Gwendal Cueff, Boris Collet, Gilles Clement, Sandrine Balzergue, Stephanie Huguet, Benoit Valot, Marc Galland, Loic Rajjou
Summary: This study examines the genetic pathways and metabolic changes in the embryo and endosperm of rice seeds during germination using a multi-omics approach. The results show that the early phase of germination is a crucial turning point, and the endosperm and embryo exhibit distinct changes at the protein and metabolite levels. The study also highlights the importance of hormonal metabolism and energy supply-related proteins in rice seed germination.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Elise Bizouerne, Julia Buitink, Benoit Ly Vu, Joseph Ly Vu, Eddi Esteban, Asher Pasha, Nicholas Provart, Jerome Verdier, Olivier Leprince
Summary: By analyzing the RNA sequences of different seed tissues, several gene modules associated with the physiological traits of seeds were identified. Dormancy is gradually released during fruit ripening, accompanied by an increase in seed vigor. Some gene modules involve gene expression regulation related to seed vigor.
Article
Biochemistry & Molecular Biology
Heng Zhang, Guang Chen, Heng Xu, Sasa Jing, Yingying Jiang, Ziwen Liu, Hua Zhang, Fulin Wang, Xiangyang Hu, Ying Zhu
Summary: Seed germination is a crucial step in plant development, and RNA-Seq was used to study gene expression in the embryo and endosperm of germinating rice seeds. A total of 14,391 differentially expressed genes (DEGs) were identified, with 7109 found in both the embryo and endosperm. The embryo-specific DEGs were enriched in plant-hormone signal-transduction pathway, while the endosperm-specific DEGs were enriched in amino acid biosynthesis pathways. Transcription-factor analysis revealed 643 differentially expressed TFs, and the knockout of OsBiP2 resulted in reduced germination rates.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Pham Anh Tuan, Tran-Nguyen Nguyen, Parneet K. Toora, Belay T. Ayele
Summary: This study comprehensively analyzed the changes in the expression patterns of plant hormone metabolism genes and the levels of their bioactive forms during barley seed development. The results showed that the levels of plant hormones were generally high in the endosperm and embryo tissues at the early stage of seed filling, but decreased during seed filling, except for ABA. The level of ABA in the embryo increased during seed filling and peaked at physiological maturity, while the endospermic ABA remained stable during seed filling. After physiological maturity, most hormones in both tissues showed low levels, except for higher levels of jasmonoyl-isoleucine and SA detected at late stage.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Multidisciplinary Sciences
Rong Liu, Jing Lu, Jiayi Xing, Mei Du, Mingxiu Wang, Lei Zhang, Yunfang Li, Chihong Zhang, Yu Wu
Summary: The germination of Polygonatum cyrtonema Hua seeds involves the regulation of plant hormones, alpha-amylase activity, and metabolites related to phenylpropanoid and flavonoid biosynthesis. Germinated seeds promote flavonoid synthesis and inhibit lignin synthesis, facilitating the germination process. Additionally, starch hydrolysis into glucose provides necessary energy for germination.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Yixuan Feng, Tai Wang, Lingtong Liu
Summary: Tomato is a major cash crop worldwide and its seed is an important model for studying genetics and developmental biology. However, the complex structure of tomato seeds poses challenges for their observation. This study presents a rapid and labor-saving clearing protocol that can efficiently clear tomato seeds at different developmental stages and enable high-resolution monitoring of mutant seeds.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Biochemistry & Molecular Biology
Joanna Rojek, Matthew R. Tucker, Michal Rychlowski, Julita Nowakowska, Malgorzata Gutkowska
Summary: Auxin plays a crucial role in plant development, with its transport mediated by various proteins including Rab and RGTB1. The disruption of vesicular traffic due to mutations in RGTB1 affects both pre and post-fertilization events, highlighting the importance of proper vesicular trafficking in sexual reproduction of flowering plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Aniko Meijer, Tim De Meyer, Klaas Vandepoele, Tina Kyndt
Summary: By investigating the expression of sRNAs in different stages of rice development, this study identified many potential regulators of rice yield and quality, highlighting their importance in optimizing rice production.
Article
Plant Sciences
Seungjun Shin, Chayanee Chairattanawat, Yasuyo Yamaoka, Qianying Yang, Youngsook Lee, Jae-Ung Hwang
Summary: In this study, the ABCA10 transporter in Arabidopsis was characterized and found to play an essential role in early seed development. ABCA10 is involved in lipid metabolism by transporting substrates to the endoplasmic reticulum (ER), and mutation or overexpression of ABCA10 causes defects in seed development or excessive accumulation of seed lipids.
Review
Plant Sciences
Rebecca A. Povilus, Mary Gehring
Summary: Seed development involves the coordination of embryo growth, embryo-nourishing endosperm, and maternal tissue. The endosperm is closely related to nutritional dynamics, developmental signaling, and imprinted gene expression. The diversity of endosperm in angiosperms deserves further examination.
CURRENT OPINION IN PLANT BIOLOGY
(2022)
Review
Plant Sciences
Luis Alonso Baez, Laura Bacete
Summary: This review provides a comprehensive guide to the latest techniques for understanding the dynamic and diverse nature of plant cell walls, emphasizing non-invasive and live-imaging strategies. The capacities of several methodologies to study cell wall composition, structure, and other aspects, developed or optimized in recent years, are described. The techniques discussed focus on Arabidopsis thaliana primary cell walls, but they can be applied to secondary cell walls and other plant species as well.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Chemistry, Physical
Etienne Couturier, Dominic Vella, Arezki Boudaoud
Summary: This study extends previous theoretical work on indentation experiments and provides formulas for deducing modulus or pressure from experimental results. These formulas are applicable to different pressure levels with realistic contact geometries.
EUROPEAN PHYSICAL JOURNAL E
(2022)
Review
Biochemistry & Molecular Biology
Adrienne H. K. Roeder, Marisa S. Otegui, Ram Dixit, Charles T. Anderson, Christine Faulkner, Yan Zhang, Maria J. Harrison, Charlotte Kirchhelle, Gohta Goshima, Jeremy E. Coate, Jeff J. Doyle, Olivier Hamant, Keiko Sugimoto, Liam Dolan, Heather Meyer, David W. Ehrhardt, Arezki Boudaoud, Carlos Messina
Summary: The open questions in plant cell biology raised by 15 experts cover a wide range of topics, including organelle maintenance, cell wall synthesis, cell division, polyploidy effects, intracellular signaling, and emergent effects of complex dynamical processes on cells, organs, and organisms.
Article
Biochemistry & Molecular Biology
Manuel Nieves-Cordones, Farrukh Azeem, Yuchen Long, Martin Boeglin, Geoffrey Duby, Karine Mouline, Eric Hosy, Alain Vavasseur, Isabelle Cherel, Thierry Simonneau, Frederic Gaymard, Jeffrey Leung, Isabelle Gaillard, Jean-Baptiste Thibaud, Anne-Alienor Very, Arezki Boudaoud, Herve Sentenac
Summary: Gas exchange in land plants is regulated by stomata, which optimize photosynthesis and limit water vapor loss. This study reveals the mechanical contributions of epidermal cells in stomatal aperture regulation. By disrupting the Arabidopsis thaliana K+ channel subunit gene AtKC1, the researchers found that the back pressure exerted by pavement cells onto guard cells is impaired, leading to larger stomatal apertures. Potassium ion transport within the leaf epidermis plays a crucial role in stomatal functioning.
Article
Multidisciplinary Sciences
Stefanie Royek, Martin Bayer, Jens Pfannstiel, Juergen Pleiss, Gwyneth Ingram, Annick Stintzi, Andreas Schaller
Summary: This study investigates the proteolytic processing and posttranslational modification of the TWS1 peptide regulator in Arabidopsis thaliana. The results demonstrate the critical role of tyrosine sulfation in mediating processing specificity and peptide maturation. The findings provide insight into the contribution of posttranslational modifications to peptide bioactivity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Jekaterina Truskina, Stefanie Bruck, Annick Stintzi, Sophy Boeuf, Nicolas M. Doll, Satoshi Fujita, Niko Geldner, Andreas Schaller, Gwyneth C. Ingram
Summary: The surface of pollen grains is strengthened by components produced by tapetum cells, which are regulated by the GASSHO receptor-like kinase pathway. The pathway is controlled by sulfated peptides and requires the action of subtilases. Three types of cells coordinate to deposit pollen wall through molecular dialogue.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Madeleine Seale, Annamaria Kiss, Simone Bovio, Ignazio Maria Viola, Enrico Mastropaolo, Arezki Boudaoud, Naomi Nakayama
Summary: This article introduces the mechanism by which plants generate motion through the absorption and release of water, using the example of the dandelion to explore the relationship between the structure and function of closing its hairy pappus. Through studying the structure and properties of the actuator cell walls, the authors identify the mechanism by which the dandelion pappus closes and develop a structural computational model to investigate its critical design features. The actuator relies on the radial arrangement of vascular bundles and surrounding tissues to coordinate movement. This simple material-based mechanism has promising biomimetic potential in robotics and functional materials.
NATURE COMMUNICATIONS
(2022)
Article
Developmental Biology
Jekaterina Truskina, Sophy Boeuf, Joan Renard, Tonni Grube Andersen, Niko Geldner, Gwyneth Ingram
Summary: During flowering plant reproduction, the tapetum and developing pollen grains are symplastically isolated from each other, and from other sporophytic tissues, by a structure called the peritapetal strip. This strip prevents the diffusion of substances from outside the cells. The formation and barrier functions of the peritapetal strip depend on two NADPH oxidases.
Review
Cell Biology
Celia Municio-Diaz, Elise Muller, Stephanie Drevensek, Antoine Fruleux, Enrico Lorenzetti, Arezki Boudaoud, Nicolas Minc
Summary: The cell wall is a thin and rigid layer that protects plant and fungal cells from mechanical stress. It is made of polysaccharides and proteins, and can change in composition and geometry during growth or infection. Recent studies have shown that the cell wall can detect and measure mechanical stress, and researchers have made progress in understanding how it strengthens to ensure surface integrity. This knowledge has important implications for cell growth and survival.
JOURNAL OF CELL SCIENCE
(2023)
Letter
Plant Sciences
Nicolas Max Doll, Eduardo Berenguer, Jekaterina Truskina, Gwyneth Ingram
Article
Multidisciplinary Sciences
Audrey Creff, Olivier Ali, Camille Bied, Vincent Bayle, Gwyneth Ingram, Benoit Landrein
Summary: Using computational simulations and experiments, the authors propose a mechanical model of seed growth where endosperm pressure directly induces growth but indirectly inhibits it through force-dependent testa wall stiffening. By combining experiments with computational modelling, the researchers provide evidence that endosperm pressure plays a dual role in seed growth, both driving growth directly and inhibiting it indirectly. Their findings suggest that developmental regulation of endosperm pressure is necessary to prevent premature reduction in seed growth rate caused by force-dependent seed coat stiffening.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Louis A. Chevalier, Mario Pinar, Remi Le Borgne, Catherine Durieu, Miguel Penalva, Arezki Boudaoud, Nicolas Minc
Summary: Hyphal tip growth in filamentous fungi is driven by the expansion of a protective cell wall (CW) secreted from apical vesicles. We found that tip growth is associated with balanced rates of CW secretion and expansion, and affecting this balance leads to immediate changes in CW thickness, mechanics, and shape. These findings provide unprecedented insights into the dynamics of CW secretion and expansion, and their role in stabilizing fungal tip growth and promoting morphogenetic plasticity.
Article
Physics, Multidisciplinary
Charles Paul Moore, Julien Husson, Arezki Boudaoud, Gabriel Amselem, Charles N. Baroud
Summary: The capture of a soft spherical particle in a rectangular slit results in a nonmonotonic pressure-flow rate relationship at low Reynolds number. Simulations demonstrate that the deformations induced by flow focus the streamlines and pressure drop to a small region, significantly increasing the flow resistance. Consequently, two regimes are observed: a flow-dominated regime where flow rate increases with pressure for small particle deformations, and an elastic-dominated regime where solid deformations block the flow.
PHYSICAL REVIEW LETTERS
(2023)
Article
Plant Sciences
Kenta C. C. Moriya, Makoto Shirakawa, Jeanne Loue-Manifel, Yoriko Matsuda, Yen-Ting Lu, Kentaro Tamura, Yoshito Oka, Tomonao Matsushita, Ikuko Hara-Nishimura, Gwyneth Ingram, Ryuichi Nishihama, Justin Goodrich, Takayuki Kohchi, Tomoo Shimada
Summary: The acquisition of new gene regulatory networks controlled by transcription factors is necessary for the evolution of special types of cells. In this study, it was found that a module formed by subfamilies Ia and IIIb basic helix-loop-helix transcription factors regulates the development of a unique sporophyte tissue, the seta, in the astomatous liverwort Marchantia polymorpha. This module also partially replaces the stomatal function in Arabidopsis thaliana, implying a common regulatory mechanism underlying setal and stomatal formation.
Article
Physics, Multidisciplinary
Chanania Steinbock, Eytan Katzav, Arezki Boudaoud
Summary: We propose a mathematical model to describe the athermal fluctuations of thin sheets and find that the structure factor follows a logarithmically corrected rational function.
PHYSICAL REVIEW RESEARCH
(2022)