Article
Cell Biology
Neil M. Neumann, Daniel M. Kim, Robert J. Huebner, Andrew J. Ewald
Summary: In this study, the process of bifurcation of mammary epithelium was investigated. The researchers observed changes in cell migration speed and the role of TGF-0 signaling in this process, revealing the mechanism behind mammary epithelial bifurcation.
JOURNAL OF CELL SCIENCE
(2023)
Article
Cell Biology
Jingqiang Wang, Wenqian Song, Ruikai Yang, Chao Li, Ting Wu, Xiao Bing Dong, Bin Zhou, Xizhi Guo, Jianfeng Chen, Zhiyong Liu, Qing Cissy Yu, Wen Li, Junfen Fu, Yi Arial Zeng
Summary: This study reveals a crucial signaling axis in which fibroblasts relay Wnt signals from endothelial cells to organize epithelial patterning. It also demonstrates the importance of different niche components in coordinating epithelial organogenesis and tissue homeostasis.
Article
Physiology
Marika Caruso, Sjanie Huang, Larissa Mourao, Colinda L. G. J. Scheele
Summary: Branching morphogenesis is the process of forming branched structures in organs such as the lung and kidney. The exact mechanisms behind branch elongation and bifurcation are still not well understood. This study presents an improved culture method for developing complex branched mammary organoids, which offers new possibilities for studying branching in the mammary gland.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Developmental Biology
Jin Man Kim, YoungJu Jo, Ju Won Jung, Kyungpyo Park
Summary: The study reported a mechanogenetic role of the actomyosin complex in branching morphogenesis, revealing the importance of force distribution for promoting the branching process, and the crucial genes regulated by YAP and TAZ for the distribution of epithelial progenitor cells in epithelial organs.
Article
Developmental Biology
Ayan T. Ray, Philippe Soriano
Summary: Loss of FGF signaling leads to defects in salivary gland branching. Disruption of Fgfr1 and Fgfr2 expression in salivary gland epithelial cells reveals their coordinated function in regulating branching. Non-canonical FGF signaling mechanisms involving cell-cell and cell-matrix adhesion play a role in salivary gland branching morphogenesis.
Article
Biochemical Research Methods
Sijie M. Tong, Navreeta K. Singh, Rastko M. Sknepnek, Andrej Kosmrlj
Summary: Epithelial tissues exhibit complex viscoelastic rheological behavior, where cells can actively tune their mechanical properties to change the overall response of the tissue. This study systematically investigated the shear and bulk responses of epithelia in both fluid and solid phases using small oscillatory shear and bulk deformations. The findings suggest that the solid-fluid transition can be controlled by applying pre-deformation to the system.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Article
Developmental Biology
Joshua A. Braunstein, Amy E. Robbins, Scott Stewart, Kryn Stankunas
Summary: Teleost fish fins consist of a series of bones laid out in a characteristic pattern, with Sonic hedgehog playing a crucial role in bone regeneration. This study investigates the function of Sonic hedgehog in ray branching during the developmental process of fins, revealing its unique mechanism in regulating branching morphogenesis.
DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Kuan Zhang, Erica Yao, Ethan Chuang, Biao Chen, Evelyn Y. Chuang, Pao-Tien Chuang
Summary: Lung branching requires coordination between the differentiation of progenitor cells and morphogenetic events, with mTORC1 signaling playing a crucial role. Loss of mTORC1 signaling in lung progenitors disrupts the formation of the conducting airways without affecting gas exchange unit development. Additionally, compromised mitochondrial capacity also leads to lung developmental defects. These findings highlight the selective utilization of energy and regulatory programs during stem cell differentiation to produce distinct structures of the mammalian lungs.
NATURE COMMUNICATIONS
(2022)
Article
Developmental Biology
Adam Packard, William H. Klein, Frank Costantini
Summary: Research has shown that Ret signaling in kidney development is crucial for maintaining tip cells in the ureteric bud. Cells genetically forced to maintain Ret expression exhibit a tip-seeking behavior and self-organize into dense clusters in organoids. This suggests that Ret-dependent cell behaviors play a significant role in normal branching morphogenesis.
Article
Food Science & Technology
Garam An, Junho Park, Whasun Lim, Gwonhwa Song
Summary: This study aimed to investigate the harmful effects of folpet on bovine mammary glands and milk production using MAC-T cells. The results showed that folpet exhibited cytotoxicity against MAC-T cells, causing apoptosis and cell death. Furthermore, folpet treatment induced oxidative stress and activated MAPK signaling pathways. This is the first report highlighting the detrimental impacts of folpet on bovine mammary glands and the dairy industry, providing insights into the intracellular mechanisms using MAC-T cells.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Multidisciplinary Sciences
Sounak Sahu, Mary E. Albaugh, Betty K. Martin, Nimit L. Patel, Lisa Riffle, Susan Mackem, Joseph D. Kalen, Shyam K. Sharan
Summary: This study utilizes an in vitro culture system of mammary organoids to reconstruct the epithelial layers of the adult mammary gland by modulating the growth factor signaling pathways. A toolkit for studying organ regeneration and ductal morphogenesis is established using in vivo and ex vivo imaging techniques. The imaging modalities allow observation of ductal branching after transplantation, providing insights into mammary gland morphogenesis.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Sanil Patel, Njeri Z. R. Sparman, Douglas Arneson, Alexandra Alvarsson, Luis C. Santos, Samuel J. J. Duesman, Alessia Centonze, Ephraim Hathaway, In Sook Ahn, Graciel Diamante, Ingrid Cely, Chung Hwan Cho, Noble Kumar Talari, Abha K. K. Rajbhandari, Leigh Goedeke, Peng Wang, Atul J. J. Butte, Cedric Blanpain, Karthickeyan Chella Krishnan, Aldons J. J. Lusis, Sarah A. A. Stanley, Xia Yang, Prashant Rajbhandari
Summary: The mammary gland ductal epithelial cells regulate cold-induced adipocyte UCP1 expression and control adipose thermogenesis through the secretion of mammokines. These findings suggest that sympathetic nerve-activated glandular epithelium plays a crucial role in adipocyte UCP1 expression and has implications for energy expenditure and subcutaneous fat loss.
Article
Cell Biology
Jessica Elswood, Scott J. Pearson, H. Ross Payne, Rola Barhoumi, Monique Rijnkels, Weston W. Porter
Summary: Through studying the differentiation and metabolism of mammary epithelial cells, it was found that mitophagy occurs selectively during the differentiation process. Inhibiting autophagy impairs cell differentiation, while the PRKN protein is necessary for cell differentiation.
Article
Biochemistry & Molecular Biology
Hakyoung You, Garam An, Hojun Lee, Whasun Lim, Gwonhwa Song
Summary: In this study, we investigated the toxic effects of bifenox on bovine mammary glands using MAC-T cells. Our findings showed that bifenox inhibited cell proliferation, disrupted the cell cycle, and caused disturbances in calcium homeostasis and mitochondrial membrane potential. We also observed hyperactivation of PI3K/AKT and MAPK signaling cascades in response to bifenox treatment.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Cell Biology
Rongze Ma, Difei Gong, Huanyang You, Chongshen Xu, Yunzhe Lu, Gabriele Bergers, Zena Werb, Ophir D. Klein, Claudia K. Petritsch, Pengfei Liu
Summary: The modulation of Integrin 01 signaling by Lgl1 is essential for directional migration, and epithelial branching in the mammary gland differs fundamentally from invertebrates.
Article
Cell Biology
Neil M. Neumann, Matthew C. Perrone, Jim H. Veldhuis, Robert J. Huebner, Huiwang Zhan, Peter N. Devreotes, G. Wayne Brodland, Andrew J. Ewald
DEVELOPMENTAL CELL
(2018)
Article
Oncology
Dan Georgess, Veena Padmanaban, Orit Katarina Sirka, Kester Coutinho, Alex Choi, Gabriela Frid, Neil M. Neumann, Takanari Inoue, Andrew J. Ewald
Review
Dermatology
Abrar Choudhury, Neil M. Neumann, David R. Raleigh, Ursula E. Lang
DERMATOLOGY AND THERAPY
(2020)
Article
Oncology
Wenjie Huang, Bernat Navarro-Serer, Yea Ji Jeong, Peter Chianchiano, Limin Xia, Claudio Luchini, Nicola Veronese, Cameron Dowiak, Tammy Ng, Maria A. Trujillo, Bo Huang, Michael J. Pfluger, Anne M. Macgregor-Das, Gemma Lionheart, Danielle Jones, Kohei Fujikura, Kim-Vy Nguyen-Ngoc, Neil M. Neumann, Vincent P. Groot, Alina Hasanain, A. Floortje van Oosten, Sandra E. Fischer, Steven Gallinger, Aatur D. Singhi, Amer H. Zureikat, Randall E. Brand, Matthias M. Gaida, Stefan Heinrich, Richard A. Burkhart, Jin He, Christopher L. Wolfgang, Michael G. Goggins, Elizabeth D. Thompson, Nicholas J. Roberts, Andrew J. Ewald, Laura D. Wood
Article
Biochemical Research Methods
Veena Padmanaban, Eloise M. Grasset, Neil M. Neumann, Andrew K. Fraser, Elodie Henriet, William Matsui, Phuoc T. Tran, Kevin J. Cheung, Dan Georgess, Andrew J. Ewald
Article
Pathology
Neil M. Neumann, Michael C. Haffner, Pedram Argani, Chia-Sui Kao, Jonathan I. Epstein
Summary: Perivascular epithelioid cell neoplasms (PEComas) of the bladder are extremely rare, with distinct histomorphologic features, female predominance, epithelioid characteristics, variable mitotic rate and cytological atypia. Immunohistochemical staining shows strong positivity for Cathepsin K, variable expression of HMB-45 and melan-A, and possible TFE3 gene rearrangement in some cases. Diagnosis of bladder PEComas requires a combination of morphologic characterization and exclusion of other diagnostic possibilities.
AMERICAN JOURNAL OF SURGICAL PATHOLOGY
(2021)
Article
Multidisciplinary Sciences
Dianna L. Ng, Gregory M. Goldgof, Brian R. Shy, Andrew G. Levine, Joanna Balcerek, Sagar P. Bapat, John Prostko, Mary Rodgers, Kelly Coller, Sandra Pearce, Sergej Franz, Li Du, Mars Stone, Satish K. Pillai, Alicia Sotomayor-Gonzalez, Venice Servellita, Claudia Sanchez San Martin, Andrea Granados, Dustin R. Glasner, Lucy M. Han, Kent Truong, Naomi Akagi, David N. Nguyen, Neil M. Neumann, Daniel Qazi, Elaine Hsu, Wei Gu, Yale A. Santos, Brian Custer, Valerie Green, Phillip Williamson, Nancy K. Hills, Chuanyi M. Lu, Jeffrey D. Whitman, Susan L. Stramer, Candace Wang, Kevin Reyes, Jill M. C. Hakim, Kirk Sujishi, Fariba Alazzeh, Lori Pham, Edward Thornborrow, Ching-Ying Oon, Steve Miller, Theodore Kurtz, Graham Simmons, John Hackett, Michael P. Busch, Charles Y. Chiu
NATURE COMMUNICATIONS
(2020)
Article
Multidisciplinary Sciences
Dianna L. Ng, Andrea C. Granados, Yale A. Santos, Venice Servellita, Gregory M. Goldgof, Cem Meydan, Alicia Sotomayor-Gonzalez, Andrew G. Levine, Joanna Balcerek, Lucy M. Han, Naomi Akagi, Kent Truong, Neil M. Neumann, David N. Nguyen, Sagar P. Bapat, Jing Cheng, Claudia Sanchez-San Martin, Scot Federman, Jonathan Foox, Allan Gopez, Tony Li, Ray Chan, Cynthia S. Chu, Chiara A. Wabl, Amelia S. Gliwa, Kevin Reyes, Chao-Yang Pan, Hugo Guevara, Debra Wadford, Steve Miller, Christopher E. Mason, Charles Y. Chiu
Summary: RNA sequencing analysis showed a muted immune response in COVID-19 patients, with differences in pathological responses between hospitalized and outpatient patients. Machine learning can aid in the accurate identification of COVID-19 disease.
Article
Pathology
Neil M. Neumann, Philip E. LeBoit, Jarish N. Cohen
Summary: Superficial angiomyxomas (SAMs) are benign cutaneous tumors that are associated with Carney complex (CC) and often show loss of PRKAR1A protein expression, which can be detected by immunohistochemistry and used as an adjunctive diagnostic tool with high specificity.
AMERICAN JOURNAL OF SURGICAL PATHOLOGY
(2022)
Editorial Material
Hematology
Neil Montgomery Neumann, Kwun Wah Wen
Article
Cell Biology
Neil M. Neumann, Daniel M. Kim, Robert J. Huebner, Andrew J. Ewald
Summary: In this study, the process of bifurcation of mammary epithelium was investigated. The researchers observed changes in cell migration speed and the role of TGF-0 signaling in this process, revealing the mechanism behind mammary epithelial bifurcation.
JOURNAL OF CELL SCIENCE
(2023)
Article
Virology
Lilly Gu, Erica Lin, Shuaitong Liu, Ning Yang, Nicholas Kurtansky, Neil M. Neumann, Joseph Stoll, Cecilia Lezcano, Melissa Pulitzer, Sarah Noor, Alina Markova, Anthony Rossi, Mark A. Dickson, Liang Deng
Summary: Multiple treatment modalities, including chemotherapy, radiation therapy, and cryotherapy, have been reported for Kaposi sarcoma (KS). A study found that combination therapy of cryotherapy and topical imiquimod can lead to complete resolution of cutaneous KS in a relatively short period of time.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Pathology
Margaret A. Black, Neil M. Neumann, Gregor Krings, Saleh Najjar, Megan L. Troxell, Aihui Wang, W. Patrick Devine, Poonam Vohra, Cynthia Gasper, Yunn-Yi Chen, Jarish N. Cohen, Gregory R. Bean
Summary: This study examined the clinicopathologic, immunohistochemical, and genetic features of 8 breast HAs and compared them to 3 mammary MECs. The results showed that both breast HA and MEC exhibited cell type-dependent expression of high- and low-molecular-weight keratins and p63, as well as genetic features such as MAML2 fusion genes. However, there were differences in the growth pattern, tumor architecture, and cell distribution between the two types of tumors. The findings provide insights into the pathogenesis of MAML2-rearranged neoplasms of the breast and highlight the overlapping genetic features of MEC and HA.
Article
Dermatology
Neil M. Neumann, Chien-Kuang Cornelia Ding, Amanda Borgen, Carlos U. Corvera, Jeffrey P. North, Amir Qorbani, Andrew E. Horvai
Summary: This article reports a rare case of primary cutaneous malignant perivascular epithelioid cell tumor (PEComa). The tumor rapidly grew from 3 cm to 12 cm within 3 months. Histological examination revealed an infiltrative neoplasm resembling undifferentiated pleomorphic sarcoma. Immunohistochemical evaluation supported the diagnosis of PEComa. Next-generation sequencing identified pathogenic gene deletions and copy number changes. This case demonstrates the rare transformation of cutaneous PEComa into a malignant sarcoma, highlighting the importance of prompt surgical management.
AMERICAN JOURNAL OF DERMATOPATHOLOGY
(2022)
Article
Endocrinology & Metabolism
Colleen Carmody, Ashley N. Ogawa-Wong, Cecilia Martin, Cristina Luongo, Marian Zuidwijk, Benjamin Sager, Travis Petersen, Adriana Roginski Guetter, Rob Janssen, Elizabeth Y. Wu, Sylvia Bogaards, Neil M. Neumann, Kaman Hau, Alessandro Marsili, Anita Boelen, J. Enrique Silva, Monica Dentice, Domenico Salvatore, Amy J. Wagers, P. Reed Larsen, Warner S. Simonides, Ann Marie Zavacki