期刊
CURRENT GENE THERAPY
卷 21, 期 4, 页码 290-298出版社
BENTHAM SCIENCE PUBL LTD
DOI: 10.2174/1566523220666201229155833
关键词
XPR1; miR-375; esophageal squamous cell carcinoma; proliferation; migration; invasion
资金
- National Natural Science Foundation of China [81502435]
- Key project of social development of Xuzhou City, China [KC17099]
The miR-375/XPR1 axis plays a regulatory role in esophageal squamous cell carcinoma, with overexpression of miR-375 inhibiting ESCC cell proliferation, invasion, and migration, and reducing the promoting effects of XPR1 overexpression. These findings provide a new potential target for precise treatment of ESCC patients.
Objective: The purpose of this study was to explore the mechanism of the miR-375/XPR1 axis in esophageal squamous cell carcinoma (ESCC) and provide a new idea for targeted therapy of ESCC. Methods: Differentially expressed genes in GEO and TCGA databases were analyzed by bioinformatics. The expression levels of miR-375 and XPR1 mRNA were detected by qRT-PCR. Protein expression of XPR1 was detected by western blot. Bioinformatics analysis and dual luciferase assay were conducted to confirm the target relationship between miR-375 and XPR1. The viability, proliferation, migration and invasion of cells in each treatment group were detected by CCK-8, colony formation, wound healing and Transwell assays. Results: Significantly down-regulated miR-375 and remarkably up-regulated XPR1 were observed in ESCC tissue and cells. Overexpression of miR-375 inhibited proliferation, invasion and migration of ESCC cells, and greatly reduced the promoting effect of XPR1 overexpression on cell proliferation, migration and invasion. Dual luciferase assay confirmed that miR-375 targeted and inhibited XPR1 expression in ESCC. Conclusion: These results demonstrate the regulatory role of the miR-375/XPR1 axis in ESCC cells and provide a new potential target for the precise treatment of patients with ESCC.
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