Review
Cell Biology
Natalie Hoffmann, Samuel King, A. Lacey Samuels, Heather E. McFarlane
Summary: The composition and synthesis of the cell wall vary among different plant species, cell types, and regions within a cell wall, but are underpinned by common cellular mechanisms.
DEVELOPMENTAL CELL
(2021)
Article
Plant Sciences
Wei Wang, Sonja Viljamaa, Ondrej Hodek, Thomas Moritz, Totte Niittyla
Summary: The role of SUS in cellulose biosynthesis in Arabidopsis has been refuted, as alternative UDP-glucose biosynthesis pathways were found to be employed.
Article
Biochemistry & Molecular Biology
Li Yu, Yoshihisa Yoshimi, Rosalie Cresswell, Raymond Wightman, Jan J. Lyczakowski, Louis F. L. Wilson, Konan Ishida, Katherine Stott, Xiaolan Yu, Stephan Charalambous, Joel Wurman-Rodrich, Oliver M. Terrett, Steven P. Brown, Ray Dupree, Henry Temple, Kristian B. R. M. Krogh, Paul Dupree
Summary: Patterned beta-galactoglucomannan (beta-GGM) is widespread in eudicot primary cell walls and exhibits similarities to xyloglucan (XyG). This study demonstrates a related role of beta-GGM and XyG in primary cell walls, opening avenues for further research on their functions.
Article
Biochemistry & Molecular Biology
Emmanuel Panteris, Anna Kouskouveli, Dimitris Pappas, Ioannis-Dimosthenis S. Adamakis
Summary: The malfunction of p60-katanin in cell plate/daughter wall formation in the fra2 Arabidopsis thaliana loss-of-function mutant was investigated. Deviations in the chemical composition of cell plate/new cell wall were identified by transmission electron microscopy (TEM), immunolabeling, and confocal microscopy. Delayed cytokinesis in the mutant results in a loss of synchronization between cell plate growth and chemical maturation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Youssef Chebli, Amir J. Bidhendi, Karuna Kapoor, Anja Geitmann
Summary: The plant cell wall serves as an important extracellular matrix that envelops cells, maintains their shape and structure, interacts with symbionts, and protects against external stresses. The assembly of this matrix is regulated by the cytoskeleton, which also plays a key role in perceiving mechanical cues and mediating intracellular responses related to cell wall structure changes. Delivery processes of cell wall precursors and their structural continuity are crucial for cell wall assembly, with various morphogenetic processes relying on cell wall assembly as a critical element.
Article
Plant Sciences
Monica De Caroli, Elisa Manno, Gabriella Piro, Marcello S. Lenucci
Summary: The study compares the secretion patterns of three members of the Arabidopsis thaliana XTH family and reveals that XTH11 and XTH33 use a conventional protein secretion pathway to reach the cell wall and plasma membrane, while XTH29 follows an unconventional protein secretion pathway towards the apoplast. The presence of a 45-amino acid extraterminal region is crucial for the correct cell wall targeting of XTH29. Furthermore, XTH29 is significantly upregulated under drought and heat stress compared to XTH11 and XTH33, indicating different involvement of these XTHs in cell wall remodeling under abiotic stress.
Article
Cell Biology
Anirban Baral, Bibek Aryal, Kristoffer Jonsson, Emily Morris, Elsa Demes, Shogo Takatani, Stephane Verger, Tongda Xu, Malcolm Bennett, Olivier Hamant, Rishikesh P. Bhalerao
Summary: The study revealed that external mechanical cues mimic the role of auxin response factors and katanin in hook formation in dicotyledonous plants. Non-canonical TMK pathway, PIN efflux carriers, and cellulose microfibrils play key roles in hook formation, regardless of the presence or absence of external mechanical cues.
DEVELOPMENTAL CELL
(2021)
Article
Biochemistry & Molecular Biology
Rosalie Cresswell, Ray Dupree, Steven P. Brown, Caroline S. Pereira, Munir S. Skaf, Mathias Sorieul, Paul Dupree, Stefan Hill
Summary: Water plays a critical role at the hemicellulose-cellulose interface in the molecular architecture of softwood secondary cell walls. After dehydration, molecular shrinkage occurs and the original conformation is not fully recovered upon rehydration. These irreversible nanostructural changes provide a basis for understanding and enhancing the properties of wood-based materials.
Article
Biochemistry & Molecular Biology
Zuopeng Xu, Yihong Gao, Chengxu Gao, Jiasong Mei, Shaogan Wang, Jiaxin Ma, Hanlei Yang, Shaoxue Cao, Yan Wang, Fengxia Zhang, Xiangling Liu, Qiaoquan Liu, Yihua Zhou, Baocai Zhang
Summary: This study reveals a mechanism by which a rice enzyme BC16 remodels GPI lipid tails and governs cell wall biomechanics. Loss of BC16 alters GPI lipid structure and disturbs the targeting of GPI-AP BC1 to the cell membrane, leading to compromised cellulosic nanofiber deposition and abnormal mechanical properties. The findings provide insights into the control of cell wall biomechanics and offer potential for breeding crops with improved support strength.
Article
Multidisciplinary Sciences
Hongcheng Zhao, Nan Sun, Lin Huang, Ruyi Qian, Xianyong Lin, Chengliang Sun, Yongguan Zhu
Summary: The molecular mechanism of root development mediated by the beneficial bacterium Azospirillum brasilense is not fully understood. A. brasilense induces extensive transcriptional changes but inhibits primary root elongation in Arabidopsis. Through analysis of root cell type-specific markers, it was found that A. brasilense does not alter the overall organization or cell division of the primary root meristem. The inhibition of root elongation is primarily due to the reduction of cell elongation, which is caused by bacterially activated peroxidase leading to cell wall cross-linking. The activation of peroxidase, in combination with the downregulation of cell wall loosening enzymes, results in an increase in cell wall thickness.
Article
Plant Sciences
Fan Wang, Zhijun Cheng, Jiachang Wang, Feng Zhang, Baocai Zhang, Sheng Luo, Cailin Lei, Tian Pan, Yongfei Wang, Yun Zhu, Min Wang, Weiwei Chen, Qibing Lin, Shanshan Zhu, Yihua Zhou, Zhichao Zhao, Jie Wang, Xiuping Guo, Xin Zhang, Ling Jiang, Yiqun Bao, Yulong Ren, Jianmin Wan
Summary: This study identified a rice mutant, dph1-1, with limited cell expansion, resulting in reduced plant height and smaller panicles and grains. The protein OsSCD2, homologous to Arabidopsis SCD2, was found to be associated with clathrin and involved in defective endocytosis and post-Golgi trafficking in the dph1-1 mutant. The mutant also displayed impaired cellulose synthesis. These findings suggest that OsSCD2 plays a crucial role in clathrin-related vesicular trafficking and is important for maintaining plant growth in rice.
Review
Biochemistry & Molecular Biology
Ying Gu, Carolyn G. Rasmussen
Summary: Building a complex structure like the cell wall is a well-orchestrated process that requires assembling many individual parts from different sources within the cell. Cell wall components are actively transported to and from the plasma membrane during diffuse growth. Motor proteins and interactions with the cytoskeleton help transport polysaccharides and enzymes to the correct location for cell wall construction, while newly formed cell walls during cytokinesis have special features.
Article
Multidisciplinary Sciences
Antri Georgiou, Simon Sieber, Chien-Chi Hsiao, Tatyana Grayfer, Jacob L. Gorenflos Lopez, Karl Gademann, Leo Eberl, Aurelien Bailly
Summary: After a century of research, it was found that the obligate beta-proteobacterial endosymbionts in leaf nodules of tropical Rubiaceae supply the alpha-D-glucose analogue (+)-streptol to their Psychotria hosts, inhibiting root growth selectively. The compound appears to specifically target meristematic root cells by affecting cell wall organization, potentially serving as an allelochemical. Moreover, the suppression of plant competitors' growth is proposed as a major factor in the formation and maintenance of the Psychotria-Burkholderia association.
SCIENTIFIC REPORTS
(2021)
Article
Plant Sciences
Lazar Novakovic, Gleb E. Yakubov, Yingxuan Ma, Antony Bacic, Kerstin G. Blank, Arun Sampathkumar, Kim L. Johnson
Summary: DEK1 is a key regulator of plant cell wall that influences the mechanical properties and regulation of cellulose synthesis. It modifies the biosynthetic properties of cellulose synthase complexes and alters the stiffness and thickness of cellulose microfibril bundles in epidermal cell walls.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Weiwei Zhang, Christopher J. Staiger
Summary: This study introduces a quantitative approach for measuring the frequency of vesicle tethering events during exocytosis in plant cells. The results demonstrate that the initial vesicle tethering step is dependent on actin and myosin XI, while microtubules do not directly affect vesicle tethering and fusion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Chao Wang, Tianwei Hu, Xu Yan, Tingting Meng, Yutong Wang, Qingmei Wang, Xiaoyue Zhang, Ying Gu, Clara Sanchez-Rodriguez, Astrid Gadeyne, Jinxing Lin, Staffan Persson, Daniel Van Damme, Chuanyou Li, Sebastian Y. Bednarek, Jianwei Pan
Article
Multidisciplinary Sciences
Shundai Li, Logan Bashline, Yunzhen Zheng, Xiaoran Xin, Shixin Huang, Zhaosheng Kong, Seong H. Kim, Daniel J. Cosgrove, Ying Gu
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Plant Sciences
Aixin Li, Mingqi Zhou, Donghui Wei, Hu Chen, Chenjiang You, Juan Lin
FRONTIERS IN PLANT SCIENCE
(2017)
Article
Multidisciplinary Sciences
Xiaoyu Zhu, Shundai Li, Songqin Pan, Xiaoran Xin, Ying Gu
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Multidisciplinary Sciences
Mingqi Zhou, Hu Chen, Donghui Wei, Hong Ma, Juan Lin
SCIENTIFIC REPORTS
(2017)
Article
Genetics & Heredity
Donghui Wei, Mingjia Liu, Hu Chen, Ye Zheng, Yuxiao Liu, Xi Wang, Shuhua Yang, Mingqi Zhou, Juan Lin
Article
Plant Sciences
Xiaoran Xin, Lei Lei, Yunzhen Zheng, Tian Zhang, Sai Venkatesh Pingali, Hugh O'Neill, Daniel J. Cosgrove, Shundai Li, Ying Gu
JOURNAL OF EXPERIMENTAL BOTANY
(2020)
Article
Plant Sciences
Xiaoyu Zhu, Frederique Tellier, Ying Gu, Shundai Li
Review
Biochemistry & Molecular Biology
Ying Gu, Carolyn G. Rasmussen
Summary: Building a complex structure like the cell wall is a well-orchestrated process that requires assembling many individual parts from different sources within the cell. Cell wall components are actively transported to and from the plasma membrane during diffuse growth. Motor proteins and interactions with the cytoskeleton help transport polysaccharides and enzymes to the correct location for cell wall construction, while newly formed cell walls during cytokinesis have special features.
Article
Biochemistry & Molecular Biology
Holly Allen, Leo Zeef, Kris Morreel, Geert Goeminne, Manoj Kumar, Leonardo D. Gomez, Andrew P. Dean, Axel Eckmann, Cinzia Casiraghi, Simon J. McQueen-Mason, Wout Boerjan, Simon R. Turner
Summary: Woody plant material has the potential to produce biofuels and other bio-based products with favorable CO(2) emissions. Recent research demonstrated the use of flexible moldable wood to generate novel structural materials. The Apple rubbery wood (ARW) disease causes increased flexibility in woody stems due to a viral infection. The symptoms of ARWV infections arise from reduced lignification in the secondary cell wall of xylem fibers, resulting in increased wood digestibility. The downregulation of PAL, the enzyme involved in lignin biosynthesis, and the accumulation of PAL-derived vasiRNAs contribute to the altered lignin in the infected wood.
Article
Plant Sciences
Xiaoran Xin, Donghui Wei, Lei Lei, Haiyan Zheng, Ian S. Wallace, Shundai Li, Ying Gu
Summary: Cellulose is crucial for plant cell walls and plays an important role in various industries such as food, paper, textiles, and biofuel. This study identified the protein kinase CPK32 as a key regulator of cellulose biosynthesis and uncovered a novel mechanism by which phosphorylation controls the stability of cellulose synthase complexes (CSCs).
Article
Plant Sciences
Mohammad Murshed, Donghui Wei, Ying Gu, Jin Wang
Summary: This study developed a computational modeling framework for microtubules in plant cells and found that the interaction between microtubules and cytoplasmic flow is crucial for their assembly and orientation.
Review
Chemistry, Applied
Xingran Kou, Dongdong Su, Fei Pan, Xiwei Xu, Qingran Meng, Qinfei Ke
Summary: This review provides a systematic discussion of the application of molecular dynamics (MD) simulations in aroma compounds (ACs)/cyclodextrins (CDs) inclusion complexes (ICs). It covers the establishment of the simulation process, parameter selection, model evaluation, and various application cases, summarizing the major achievements and challenges of this method.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Applied
Haoyu Yu, Quanbin Zhang, Ammad Ahmad Farooqi, Jing Wang, Yang Yue, Lihua Geng, Ning Wu
Summary: Brown algae are rich in fucoidan, which has been found to have anti-cancer and anti-metastasis effects. Fucoidan inhibits tumor cell growth, proliferation, and metastasis, and also promotes immune responses in the tumor microenvironment.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Applied
Liang Dong, Yanan Li, Hailin Cong, Bing Yu, Youqing Shen
Summary: Gene therapy is a revolutionary treatment that requires suitable vectors for protecting and releasing exogenous nucleic acids in target cells. Chitosan, as a non-viral vector, has gained attention due to its good biocompatibility and ability to load large amounts of nucleic acids. This paper summarizes the potential of chitosan and its derivatives as gene delivery vector materials, discusses factors influencing transfection efficiency, performance evaluation, ways to optimize infectious efficiency, and current research development directions. It also provides an outlook on the future prospects of chitosan.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Ana Iglesias-Mejuto, Beatriz Magarinos, Tania Ferreira-Goncalves, Ricardo Starbird-Perez, Carmen Alvarez-Lorenzo, Catarina Pinto Reis, Ines Ardao, Carlos A. Garcia-Gonzalez
Summary: This study developed a novel processing strategy to manufacture drug-loaded and personalized aerogels with nanostructures. The aerogels demonstrated bioactivity and antimicrobial effects, promoting bone regeneration and preventing infections in bone tissue engineering.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Zhenxu Shi, Dimei Yang, Yan Zhou, Xinyu Chen, Lin Gan, Jin Huang
Summary: This study proposes a micro-assembly method to improve the photoluminescent properties of crystalline cellulose nanocrystals (CNCs) by organizing them within a sub-micrometer-sized metal-organic framework and coating with TiO2. The TiO2 coating prevents CNC assembly breakdown and allows information to be revealed using screenprinted labels for anti-counterfeiting purposes.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Xuejiao Liu, Bin Fu, Jierong Chen, Zhenyu Sun, Dongdong Zheng, Zhonghua Li, Bing Gu, Ying Zhang, Haojie Lu
Summary: Liver disease is a major cause of global mortality, and identifying biomarkers for diagnosing its progression is crucial for improving outcomes. Targeted mass spectrometry is a powerful tool for verifying biomarker candidates and clinical applications, particularly for glycoproteins translation. However, the limitation of analyzing only one sample per run has become apparent. In this study, a high-throughput intact N-glycopeptides quantification strategy was developed, allowing the validation of 20 samples per run with an average analysis time of 3 minutes per sample. The strategy was applied in a cohort of 461 serum samples and identified a panel of 10 IgG N-glycopeptides that have strong clinical utility in evaluating the severity of liver disease.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Qian Wang, Hao Jiang, Hongli Zhang, Weiqiao Lu, Xiao Wang, Wenfeng Xu, Jia Li, Youjing Lv, Guoyun Li, Chao Cai, Guangli Yu
Summary: This study proposes a novel strategy of antibody-beta-glucan conjugates (AGC) to enhance the antitumor immune response to immune checkpoint blockade (ICB) therapy. AGC demonstrated powerful tumor suppression and promoted interaction between tumor cells and dendritic cells (DCs), thereby enhancing immunotherapeutic benefits.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Chaeyeon Lee, Sojeong Lee, Sang Wook Kang
Summary: The study aims to enhance the properties of porous membranes by addressing the limitations associated with phase separation. By using cellulose acetate and citric acid, the researchers were able to fabricate membranes with improved mechanical strength and thermal stability. The cross-linking effect of citric acid resulted in a more uniform pore structure and higher porosity.
CARBOHYDRATE POLYMERS
(2024)
Review
Chemistry, Applied
Tejaswini Kolipaka, Giriraj Pandey, Noella Abraham, Dadi A. Srinivasarao, Rajeev Singh Raghuvanshi, P. S. Rajinikanth, Vidya Tickoo, Saurabh Srivastava
Summary: This review focuses on the design and application of polysaccharide-based hydrogel wound dressings, highlighting aspects such as biocompatibility, biodegradability, drug entrapment, moisturizing ability, swelling, and mechanical properties. Additionally, various crosslinking methods and recent developments in stimuli-responsive hydrogels are discussed.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Anqi Xu, Nan Zhang, Shixing Su, Hongyu Shi, Daoqiang Lu, Xifeng Li, Xin Zhang, Xin Feng, Zhuohua Wen, Gengwu Ma, Mengshi Huang, Chi Huang, Yuqi Hu, Hao Yuan, Qinwen Liu, Daogang Guan, Jun Wang, Chuanzhi Duan
Summary: The study presents a one-pot radical polymerization method to fabricate a hydrogel with adhesive properties, which can effectively treat bacterial-infected diabetic wounds and accelerate wound healing.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Montarop Yamabhai, Munthipha Khamphio, Thae Thae Min, Chai Noy Soem, Nguyen Cao Cuong, Waheni Rizki Aprilia, Krisanai Luesukprasert, Karsidete Teeranitayatarn, Atthaphon Maneedaeng, Tina R. Tuveng, Silje B. Lorentzen, Simen Antonsen, Paiboon Jitprasertwong, Vincent G. H. Eijsink
Summary: The study investigates the bioconversion of chitosan into soluble anti-inflammatory chitosan oligosaccharides (CHOS) using an enzyme. The results show that the generated CHOS have anti-inflammatory activity, but the magnitude of the activity depends on the substrate and production process. Different methods of dissolving chitosan also affect the properties of CHOS. The study highlights the importance of quality assurance in CHOS preparations.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Wen Cao, Jiangping Wu, Xinya Zhao, Zixu Li, Jie Yu, Taili Shao, Xuefeng Hou, Lutan Zhou, Chunfei Wang, Guodong Wang, Jun Han
Summary: In this study, a water-soluble polysaccharide (PLP1) was successfully isolated and purified from Pueraria lobata. It was found that PLP1 was composed of specific glycosidic units and exhibited a better free radical-scavenging ability. Moreover, PLP1 effectively protected the liver against acute alcoholic liver disease (ALD) in mice.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Ya Su, Yaqian Liu, Xueyan Hu, Yueqi Lu, Jinyuan Zhang, Wenbo Jin, Wang Liu, Yan Shu, Yuen Yee Cheng, Wenfang Li, Yi Nie, Bo Pan, Kedong Song
Summary: A 3D printed scaffold based on carbon dots-curcumin nano-drug release has been developed for drug delivery after breast cancer surgery. The scaffold showed effective inhibition of tumor growth, antibacterial activity, and promotion of wound healing, making it a promising approach for preventing tumor recurrence.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Ekaterina Sokolova, Diane Jouanneau, Antonin Chevenier, Murielle Jam, Nathalie Desban, Pierre Colas, Elizabeth Ficko-Blean, Gurvan Michel
Summary: Carrageenans, a compound synthesized in red algae, have various biological properties and are valuable in the pharmaceutical and cosmetic industries. Their fine structure affects wound healing, oxidative processes, hemostasis, and inflammation. Enzymatic modification of carrageenans produces oligosaccharides that bind to natural human serum antibodies and specific antibodies, showing potential for therapeutic applications.
CARBOHYDRATE POLYMERS
(2024)
Article
Chemistry, Applied
Yan Shi, Miao Yu, Kaijin Qiu, Tiantian Kong, Chunjing Guo, Wenxue Zhang, Daquan Chen, Ming Kong
Summary: In this study, functionalized transfersomes were developed to co-deliver doxorubicin and 1MT towards primary tumors and tumor draining lymph nodes via transdermal administration using microneedles. The results showed that the nano-complexed microneedles exhibited a stronger suppression in tumor growth compared to the intravenous group.
CARBOHYDRATE POLYMERS
(2024)