期刊
BIOSENSORS & BIOELECTRONICS
卷 170, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2020.112656
关键词
Coronavirus; Airborne virus; Airborne virus monitoring; Aerosol-to-hydrosol sampling; Electrostatic air sampler; Virus enrichment
类别
资金
- BioNano Health-Guard Research Center - Ministry of Science and ICT (MSIT) of Korea as Global Frontier Project [H-GUARD_2013M3A6B2078959]
- National Research Foundation of Korea [4199990314094] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Point-of-care risk assessment (PCRA) for airborne viruses requires a system that can enrich low-concentration airborne viruses dispersed in field environments into a small volume of liquid. In this study, airborne virus particles were collected to a degree above the limit of detection (LOD) for a real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR). This study employed an electrostatic air sampler to capture aerosolized test viruses (human coronavirus 229E (HCoV-229E), influenza A virus subtype H1N1 (A/H1N1), and influenza A virus subtype H3N2 (A/H3N2)) in a continuously flowing liquid (aerosol-to-hydrosol (ATH) enrichment) and a concanavalin A (ConA)-coated magnetic particles (CMPs)-installed fluidic channel for simultaneous hydrosol-to-hydrosol (HTH) enrichment. The air sampler's ATH enrichment capacity (EC) was evaluated using the aerosol counting method. In contrast, the HTH EC for the ATH-collected sample was evaluated using transmission-electron-microscopy (TEM)-based image analysis and real-time qRT-PCR assay. For example, the ATH EC for HCoV-229E was up to 67,000, resulting in a viral concentration of 0.08 PFU/mL (in a liquid sample) for a viral epidemic scenario of 1.2 PFU/m(3) (in air). The real-time qRT-PCR assay result for this liquid sample was non-detectable however, subsequent HTH enrichment for 10 min caused the non -detectable sample to become detectable (cycle threshold (CT) value of 33.8 +/- 0.06).
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