Article
Chemistry, Multidisciplinary
Jun-Hee Park, Zhiquan Song, Ji-Hong Bong, Hong-Rae Kim, Moon-Ju Kim, Kyung-Hak Choi, Seung-Shick Shin, Min-Jung Kang, Do Young Lee, Jae-Chul Pyun
Summary: Switching peptides were designed as reversible binding antibodies for different antigens and source animals. An electrochemical one-step immunoassay using switching peptides labeled with ferrocene was conducted for quantitative measurement of analytes. The feasibility of this method was assessed by analyzing electrochemical properties and the factors affecting the amperometry of switching peptides labeled with ferrocene. The applicability of the electrochemical immunoassay for medical diagnosis of human hepatitis B surface antigen was evaluated.
Article
Biochemical Research Methods
Hong-Rae Kim, Ji-Hong Bong, Tae-Hun Kim, Seung-Shick Shin, Min-Jung Kang, Won-Bo Shim, Do Young Lee, Dong Hee Son, Jae-Chul Pyun
Summary: A one-step homogeneous immunoassay using the switching peptide H2 was developed for rapid detection of influenza viruses A and B. This method eliminates the need for washing steps and can be conducted in solution, with graphene used as a fluorescence quencher to enhance sensitivity.
Article
Chemistry, Analytical
Tae-Hun Kim, Ji-Hong Bong, Hong-Rae Kim, Won-Bo Shim, Min-Jung Kang, Jae-Chul Pyun
Summary: A one-step immunoassay using antibody complex with switching peptides is proposed for the rapid detection of OTA. Quantitative analysis of target analytes can be achieved by measuring the fluorescence signals of switching peptides without any washing steps. The direct dilution method is found to be effective for detecting OTA in wines, with a significantly reduced total analysis time.
JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY
(2022)
Article
Chemistry, Analytical
Chang Kyu Lee, Jaeyong Jung, Hong-Rae Kim, Ji-Hong Bong, Tae-Hun Kim, Jun-Hee Park, Soonil Kwon, Min-Jung Kang, Jae-Chul Pyun
Summary: A one-step immunoassay was developed for detecting five types of food-poisoning-related bacteria using a switching peptide and antibodies isolated from unimmunized horse serum. This method eliminates the need for washing steps and allows microbial detection by quantifying fluorescence signals.
Article
Chemistry, Analytical
Keine Nishiyama, Mao Fukuyama, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Akihide Hibara, Manabu Tokeshi
Summary: A non-competitive fluorescence polarization immunoassay (FPIA) using a Fab fragment was developed for large molecule quantification. The method, tested with C-reactive protein (CRP), achieved successful quantitative analysis without the need for sample pretreatment and with high sensitivity. The simplified procedure involved one-step mixing without the need for washing operations, enabling rapid protein quantification.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Nanoscience & Nanotechnology
Ye Wang, Jiongdong Zhao, Yu Zhu, Shurong Dong, Yang Liu, Yijun Sun, Liling Qian, Wenting Yang, Zhen Cao
Summary: This study presents integrated nanorod arrays on microfluidic chips for fast and sensitive flow-through immunoassays of physiologically relevant macromolecules. The plasmonic structure significantly improves the detection limit of cTnI assay and enables simultaneous detection of multiple cancer biomarkers. This ultrasensitive detection is achieved through large surface area for antibody conjugation and metal-enhanced fluorescent signals.
MICROSYSTEMS & NANOENGINEERING
(2021)
Article
Chemistry, Analytical
Benita Johannsen, Michal Karpisek, Desiree Baumgartner, Vanessa Klein, Nagihan Bostanci, Nils Paust, Susanna M. Frueh, Roland Zengerle, Konstantinos Mitsakakis
Summary: This study introduces a simple and fast one-step heterogeneous immunoassay method that can be adapted to miniaturized, automated point-of-care systems. It utilizes magnetic and fluorescent beads for capture and detection, successfully applied in the detection of CRP and oral inflammation markers, showcasing multiplexing capacity.
ANALYTICA CHIMICA ACTA
(2021)
Article
Immunology
Francesca Falconi-Agapito, Karen Kerkhof, Xiomara Merino, Diana Bakokimi, Fiorella Torres, Marjan Van Esbroeck, Michael Talledo, Kevin K. Arien
Summary: This study evaluated the discriminatory potential of 20 peptides identified using a flavivirus proteome-wide microarray and established a multiplex peptide immunoassay for detecting dengue virus infection. The findings have significant implications for the management of cases in a world with increasing exposure to arthropod-borne flaviviruses.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Xianjin Zhou
Summary: A simple and rapid one-step test was developed to detect antibodies against the SARS-CoV2 N nucleocapsid protein. The test demonstrated superior sensitivity and specificity, providing a universal method for tracking virus infection in both humans and animal reservoirs.
Article
Chemistry, Analytical
Xiaolan Yang, Xiangyu Li, Qingguo He, Yanbin Ding, Bin Luo, Qiuju Xie, Jiahao Chen, Yue Hu, Zhaohong Su, Xiaoli Qin
Summary: A facile synthetic method of TEOA-capped PtNPs is developed for electrochemiluminescent and colorimetric immunoassay of SARS-CoV spike proteins. The PtNPs exhibit high sensitivity and stability in the immunoassay of SARS-CoV S-protein.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Xiaoxiao Xing, Qingyu Lv, Chunyu Sun, Jia Song, Zhixin Chen, Yong Jiang, Ye Wang, Yongqiang Jiang, Zhifei Wang
Summary: In this study, PEG segment-functionalized polystyrene microspheres were successfully prepared by one-step copolymerization method. The results showed that the non-specific adsorption capacity of the microspheres decreased with the increase of PEOVB dosage, and the adsorption capacity of BSA declined by 97.9%. The donor microspheres prepared had the highest signal-to-noise ratio when the PEOVB content was 10 wt%.
NEW JOURNAL OF CHEMISTRY
(2023)
Article
Chemistry, Medicinal
Xinggui Yang, Xu Chen, Junfei Huang, Yijiang Chen, Wenlin Zheng, Wei Chen, Huijuan Chen, Shiguang Lei, Shijun Li
Summary: A simple, specific, low-cost, and highly sensitive detection method for Mycobacterium tuberculosis (MTB) was developed using multiplex loop-mediated isothermal amplification combined with a label-based lateral flow immuno-assay biosensor (mLAMP-LFIA). The mLAMP-LFIA method, targeting the IS6110 and mpb64 genes, showed a detection limit of 100 fg per reaction for MTB DNA, and a sensitivity of 100% and specificity of 98.04% in preliminary clinical evaluations. This method has the potential to be used as a screening and/or diagnostic tool for MTB in various settings.
ACS INFECTIOUS DISEASES
(2023)
Article
Chemistry, Analytical
Yuxing Shi, Jiuchuan Guo, Jinhong Guo
Summary: In this study, an intelligent system combining centrifugal microfluidic and Alphalisa immunoassay was investigated for early detection of pepsinogen. The system achieved rapid and highly sensitive detection of pepsinogen I and II in whole blood without the need for blood pretreatment. The portable system showed high sensitivity and accuracy, making it a potential tool for on-chip point-of-care testing.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Multidisciplinary
Min-Cheng Chien, Yi Kai Lin, Yong Liao, Szu-Hsuan Chen, Yen-Wei Chen, Chien-Yu Liang, Vijayasimha Molakaseema, Sodio C. N. Hsu, Chun-Cheng Lin, Hui-Ting Chen, Chai-Lin Kao
Summary: A one-step synthesis method was used to prepare preloaded diaminobenzoate resin. The method enables the synthesis of pure products in high yield without the need for purification steps, except for histidine. It can also be used for solid-phase peptide synthesis and the preparation of unusual amino acid derivatives, with good yields.
Article
Chemistry, Physical
Jin Lei, Song Sun, Yuchen Li, Ping Xu, Chang Liu, Shaozhong Chang, Genglai Yang, Shuang Chen, Wei Fa, Di Wu, Ai-Dong Li
Summary: A resistive random-access memory based on a nanoporous zinc-based hydroquinone thin film was created with a Pt/ZnHQ/Ag sandwich structure. These devices exhibit electroforming-free bipolar resistive switching characteristic with lower operation voltages and higher on/off ratio above 10(2). The nanoporous hybrid devices also show multilevel storage capability and excellent endurance/retention properties. The connection and disconnection of Ag conductive filaments in the nanoporous Zn-HQ thin film follow the electrochemical metallization mechanism, which is facilitated by the existence of nanopores.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Oncology
Jung Hwan Ji, Soong June Bae, Seul-Gi Kim, Min Hwan Kim, Gun-Min Kim, Joohyuk Sohn, Joon Jeong, Jee Hung Kim, Sung Gwe Ahn
Summary: This study investigated the impact of different doses of carboplatin on pathologic complete response rate, grade 3/4 anaemia occurrence, and transfusion rate in patients treated with neoadjuvant TCHP. The results showed that carboplatin at AUC5 has comparable cytotoxic effects to carboplatin at AUC6 and is associated with fewer complications related to anaemia in HER2+ breast cancer patients.
Article
Environmental Sciences
Yu-Jin Jeong, Chang-Ung Kim, Kyung-Soo Lee, Ji Hyung Kim, Seo Young Park, Ahn Young Jeong, Jun Bong Lee, Doo-Jin Kim, Young-Jun Park, Moo-Seung Lee
Summary: In this study, it was found that PM-isolated Pseudomonas stutzeri (PMPS) induces higher levels of inflammatory responses in lung epithelial cells, alveolar cells, and macrophages compared to Pseudomonas stutzeri (PS). The pathogenicity of PMPS is mediated by Toll-like receptors (TLRs) signaling pathways, which also regulate the expression of the antibiotic peptide beta-defensin 3 (BD3) that suppresses PMPS proliferation. Furthermore, pretreatment with PM enhances inflammatory responses and tissue damage caused by PMPS, while reducing BD3 expression.
ENVIRONMENTAL POLLUTION
(2023)
Article
Medicine, General & Internal
Yuna Kim, Sung Hwan Yoo, Jaeyoung Chun, Jie-Hyun Kim, Young Hoon Youn, Hyojin Park
Summary: This study found that exposing patients to virtual reality (VR) prior to endoscopic procedures can reduce their anxiety levels, leading to less discomfort and incomplete procedures, as well as decreased use of sedative medication. The proportion of patients with high anxiety significantly decreased in the VR group compared to the control group, and patients in the VR group reported higher satisfaction with sedation.
YONSEI MEDICAL JOURNAL
(2023)
Article
Nutrition & Dietetics
Mi Jung Kwon, Jae Yong Park, Sung Gyun Kim, Jwa-Kyung Kim, Hyun Lim, Joo-Hee Kim, Ji Hee Kim, Seong-Jin Cho, Eun Sook Nam, Ha Young Park, Nan Young Kim, Ho Suk Kang
Summary: Using nationwide data from the Korean National Health Insurance Service-Health Screening Cohort database, this study aimed to explore the link between gout and incident osteoporosis/osteoporotic fractures. The results showed that gout patients had a slightly higher risk of developing osteoporosis compared to controls, but no significant association was found between gout and incident osteoporotic fractures. This study provides insights into the health implications of gout in the Korean population.
Article
Medicine, General & Internal
Dong-Chan Eun, Yong-Ho Lee, Jin-Oh Park, Kyung-Soo Suk, Hak-Sun Kim, Seong-Hwan Moon, Si-Young Park, Byung-Ho Lee, Sang-Jun Park, Ji-Won Kwon, Sub-Ri Park
Summary: In this retrospective study, the clinical and radiological outcomes of patients with multilevel lumbar stenosis who underwent BESS and microscopic ULBD were compared. The results showed that the BESS group had better clinical outcomes, with significant improvements in back and leg pain scores and medication use. Radiological outcomes were also superior in the BESS group. The short-term follow-up period demonstrated the efficacy of multilevel decompression with the BESS technique.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Chemistry, Physical
Juchan Lee, Myung Joon Kim, InverstigationByeong Geun Jeong, Chan Kwon, Yumin Cha, Soo Ho Choi, Ki Kang Kim, Mun Seok Jeong
Summary: The study investigates the effect of sulfur vacancies on the electrical properties of MoS2. After generating sulfur vacancies through treatment with hydrazine, the density of shallow traps increased by a factor of 4, whereas that of deep-level traps increased by a factor of 200. These results indicate that sulfur vacancies induce both deep- and shallow-level traps, with a higher trap density at the deep level.
APPLIED SURFACE SCIENCE
(2023)
Article
Gastroenterology & Hepatology
Hyo-Joon Yang, Young-Il Kim, Ji Yong Ahn, Kee Don Choi, Sang Gyun Kim, Seong Woo Jeon, Jie-Hyun Kim, Sung Kwan Shin, Hyuk Lee, Wan Sik Lee, Gwang Ha Kim, Jae Myung Park, Woon Geon Shin, Il Ju Choi
Summary: This study validated the effectiveness of the eCura system in undifferentiated-type early gastric cancer, and found that close follow-up without additional treatment can be considered for low-risk patients, while additional surgery is recommended for intermediate- and high-risk patients.
Article
Cell & Tissue Engineering
Youn Keong Cho, Hyun-Kyung Kim, Soon Sung Kwon, Su-Hee Jeon, June-Won Cheong, Ki Taek Nam, Han-Soo Kim, Sinyoung Kim, Hyun Ok Kim
Summary: To address the blood shortage issue, scientists have been exploring the use of human-induced pluripotent stem cells (hiPSCs) to generate red blood cells (RBCs) in vitro. In this study, hiPSCs were established from peripheral blood, cord blood, and bone marrow, and it was found that peripheral blood and cord blood were the most reliable sources for RBC production in vitro. These findings will aid in the selection of optimal hiPSC lines for clinical RBC production in the future.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Medicine, General & Internal
Oh Chan Kwon, Yuna Kim, Jaeyoung Chun, Kyungdo Han, Min-Chan Park, Ryul Kim, Jie-Hyun Kim, Young Hoon Youn, Hyojin Park
Summary: This study aimed to assess whether the presence of immune-mediated inflammatory diseases (IMIDs) confers a higher risk of depression and anxiety in patients with type 2 diabetes (T2DM). The results showed that the existence of gut, joint, and skin IMIDs was associated with a higher risk of depression and anxiety. Patients with multiple IMIDs had a higher risk than those with only one.
FRONTIERS IN MEDICINE
(2023)
Article
Chemistry, Medicinal
Si-Eun Oh, Jie-Hyun Kim, Hee-Jong Shin, Seong-Ah Kim, Chan-Kee Park, Hae-Young Lopilly Park
Summary: We investigate the changes in extracellular matrix (ECM) and biomechanical properties of the sclera after systemic hypotension induced by oral hydrochlorothiazide. Angiotensin II (AngII) receptor levels and ECM components were evaluated, along with the effect of losartan inhibition and its impact on retinal ganglion cells (RGCs). The results show that AngII plays a role in scleral fibrosis after systemic hypotension, and inhibiting AngII could modulate the tissue properties of the sclera, providing protection for RGCs.
Article
Gastroenterology & Hepatology
Sang Jo Han, Su Jung Baik, Young Hoon Yoon, Jie Hyun Kim, Hye Sun Lee, Soyoung Jeon, Hyojin Park
Summary: This study aimed to compare the risk of metabolic syndrome and fatty liver diseases between gastric cancer survivors and non-cancer subjects. The results showed that gastric cancer survivors who underwent surgical treatment had lower risks of metabolic syndrome, fatty liver by ultrasonography, and metabolic dysfunction-associated fatty liver disease (MAFLD) than non-cancer subjects. However, there were no significant differences in the risks between non-surgical gastric cancer survivors and non-cancer subjects.
KOREAN JOURNAL OF GASTROENTEROLOGY
(2023)
Article
Oncology
Yuna Kim, Yoo Jin Bae, Jie-Hyun Kim, Hyunki Kim, Su-Jin Shin, Da Hyun Jung, Hyojin Park
Summary: Trastuzumab-resistant gastric cancer cells exhibit an epithelial-mesenchymal transition (EMT)-like phenotype, and trastuzumab resistance is promoted by the Wnt/beta-catenin signaling pathway. The Wnt/beta-catenin pathway is a key signaling pathway for trastuzumab resistance in gastric cancer cells.
Article
Chemistry, Analytical
Jun-Hee Park, Ga-Yeon Lee, Zhiquan Song, Ji-Hong Bong, Hong-Rae Kim, Min-Jung Kang, Jae-Chul Pyun
Summary: An electrochemical immunoassay method based on redox cycling was developed using vertically paired electrodes (VPEs) made of PEDOT:PSS as an electrode material and parylene-C as a dielectric layer. Various electrochemical properties of PEDOT:PSS were analyzed for the redox reaction of TMB at different pH conditions, and the factors affecting the sensitivity of redox cycling based on VPEs were investigated. Computer simulation was performed to explore the fabrication limitations of VPEs, and the redox cycling method was successfully applied to the medical diagnosis of hHCV. The sensitivity of the redox cycling method was compared with other conventional assay methods.
Article
Oncology
Chang Gon Kim, Min Hwan Kim, Jee Hung Kim, Seul-Gi Kim, Gun Min Kim, Tae Yeong Kim, Won-Ji Ryu, Jee Ye Kim, Hyung Seok Park, Seho Park, Young Up Cho, Byeong Woo Park, Seung Il Kim, Joon Jeong, Joohyuk Sohn
Summary: This study analyzed data from postmenopausal women with HR-positive, HER2-negative ABC who were treated with CDK4/6 inhibitors and found that on-treatment dNLR predicted progression-free survival (PFS). Flow cytometry analysis revealed potential systemic immune responses induced by CDK4/6 inhibitors, including prevention of T cell exhaustion and reduction of myeloid-derived suppressor cell frequency.
BREAST CANCER RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Joyeta Mahmud, Hien Thi My Ong, Eda Ates, Hong Seog Seo, Min-Jung Kang
Summary: This study investigated the secretome protein profile of induced-hypertrophy cardiomyocytes to identify next-generation biomarkers for AMI diagnosis and management. The results showed that 14-3-3 protein-zeta played an important role in cardiac hypertrophy and cardiovascular disorders and had the potential to be a novel biomarker and therapeutic strategy.
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)