Article
Biophysics
Riccardo Funari, Hidehiro Fukuyama, Amy Q. Shen
Summary: A multiplex nanoplasmonic biosensor has been developed to capture the humoral response against multiple antigens, providing a cost-effective and efficient alternative for serological analysis. The platform has demonstrated successful profiling of multiple serum antibodies and can be integrated with microfluidics for high throughput screening in vaccine development.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Medicine, General & Internal
Peter Chen, Ajay Nirula, Barry Heller, Robert L. Gottlieb, Joseph Boscia, Jason Morris, Gregory Huhn, Jose Cardona, Bharat Mocherla, Valentina Stosor, Imad Shawa, Andrew C. Adams, Jacob Van Naarden, Kenneth L. Custer, Lei Shen, Michael Durante, Gerard Oakley, Andrew E. Schade, Janelle Sabo, Dipak R. Patel, Paul Klekotka, Daniel M. Skovronsky
Summary: LY-CoV555, a neutralizing antibody, showed promising results in reducing viral load, improving symptoms, and lowering the risk of hospitalization among patients with mild or moderate Covid-19. While one of the doses appeared to accelerate the decline in viral load, others did not show significant effects by day 11.
NEW ENGLAND JOURNAL OF MEDICINE
(2021)
Review
Multidisciplinary Sciences
Miriam Merad, Catherine A. Blish, Federica Sallusto, Akiko Iwasaki
Summary: Considerable research effort has been focused on deciphering the immune response to SARS-CoV-2 infections and understanding the pathophysiology of COVID-19, including Long Covid syndrome. The hope is that knowledge gained from this research will be applied to studies of inflammatory processes in critical and chronic illnesses in the future.
Article
Infectious Diseases
Chang-Sheng Xia, Minghua Zhan, Yudong Liu, Zhi-Hong Yue, Ying Song, Feifei Zhang, Hui Wang
Summary: This study assessed the levels of protective antibodies in SARS survivors with and without the COVID-19 vaccine, and found that SARS survivors have protective antibodies against SARS-CoV-2 even without vaccination, and generate a stronger antibody response after vaccination compared to controls.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Allergy
Qing Lei, Yang Li, Hong-yan Hou, Feng Wang, Zhu-qing Ouyang, Yandi Zhang, Dan-yun Lai, Jo-Lewis Banga Ndzouboukou, Zhao-wei Xu, Bo Zhang, Hong Chen, Jun-biao Xue, Xiao-song Lin, Yun-xiao Zheng, Zong-jie Yao, Xue-ning Wang, Cai-zheng Yu, He-wei Jiang, Hai-nan Zhang, Huan Qi, Shu-juan Guo, Sheng-hai Huang, Zi-yong Sun, Sheng-ce Tao, Xiong-lin Fan
Summary: The study found that combining nucleic acid testing with serological testing significantly increases the detection sensitivity of asymptomatic infections. Asymptomatic individuals mainly produce IgM and IgG antibodies against S1 and N proteins, with S1-specific IgM antibodies potentially serving as early diagnostic biomarkers. Some asymptomatic individuals do not produce neutralizing antibodies, and these antibodies gradually disappear within two months.
Article
Biophysics
Liping Huang, Ying Li, Changyou Luo, Youqian Chen, Nadia Touil, Hicham-El Annaz, Shaoqi Zeng, Tang Dang, Jiawei Liang, Wenjun Hu, Hao Xu, Jiasheng Tu, Lin Wang, Yan Shen, Gang L. Liu
Summary: Researchers have developed a NanoPISA platform for evaluating the effectiveness of COVID-19 vaccines. The platform can rapidly detect SARS-CoV-2 NA in serum samples and yields results comparable to other commonly used detection methods.
BIOSENSORS & BIOELECTRONICS
(2022)
Review
Medicine, General & Internal
Rosanna W. Peeling, David L. Heymann, Yik-Ying Teo, Patricia J. Garcia
Summary: Diagnostics play a crucial role in the response to the COVID-19 pandemic. Molecular tests like PCR are recommended for confirming diagnosis in symptomatic individuals, while antigen rapid detection tests have the advantage of being easier to perform with faster results and lower cost. Antibody tests can inform public policy but should not be used as proof of immunity. All three types of tests continue to be important in transitioning from pandemic response to control.
Article
Chemistry, Multidisciplinary
Vincenzo Mazzaracchio, Mauricio Rios Maciel, Tatiana Porto Santos, Kazumi Toda-Peters, Amy Q. Shen
Summary: A duplex electrochemical immunosensor is developed for simultaneous detection of anti-nucleocapsid IgG (N-IgG) and anti-spike IgG (S-IgG) using a two-working electrode system and differential pulse voltammetry. The sensor exhibits enhanced performance by modifying the screen-printed electrodes with carbon black and electrodeposited gold nanoflowers. It achieves a wide linear range and low detection limits for N-IgG and S-IgG detection in serum samples. Integrated into a microfluidic device, the duplex immunosensor enables high throughput screening for COVID-19 and other infectious diseases with significantly reduced detection time.
Article
Virology
Angela Huynh, Donald M. Arnold, James W. Smith, Jane C. Moore, Ali Zhang, Zain Chagla, Bart J. Harvey, Hannah D. Stacey, Jann C. Ang, Rumi Clare, Nikola Ivetic, Vasudhevan T. Chetty, Dawn M. E. Bowdish, Matthew S. Miller, John G. Kelton, Ishac Nazy
Summary: COVID-19 is a global pandemic caused by SARS-CoV-2. The study found that anti-SARS-CoV-2 antibodies were detected in recovered individuals up to 180 days post-symptom onset, indicating a humoral immune response to the virus. Serological surveys are important for assessing population-level immunity based on RT-PCR-determined infection numbers.
Review
Biochemistry & Molecular Biology
Pravas Kumar Baral, Jiang Yin, Michael N. G. James
Summary: Researchers worldwide are working on developing prophylactic and therapeutic interventions to combat the COVID-19 crisis, with a focus on antibody-based therapies targeting the viral spike protein, particularly monoclonal antibodies.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Virology
Fanfan Zeng, Mengjun Wu, Jinbiao Wang, Jianyu Li, Guoyun Hu, Lin Wang
Summary: This study found that the positive rates of IgM antibody in convalescent COVID-19 patients were similar between males and females, while the positive rates of IgG antibody were nearly the same. There was a significant difference in the levels of IgG antibodies between different age groups, with older patients maintaining a higher level even after 1 year of recovery.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xin Xu, Sheng Nie, Yanqun Wang, Quanxin Long, Hong Zhu, Xiaoyong Zhang, Jian Sun, Qinglang Zeng, Jincun Zhao, Li Liu, Ling Li, Ailong Huang, Jinlin Hou, Fan Fan Hou
Summary: According to a study in Hubei, China, the dynamics of neutralizing antibody (NAbs) response to SARS-CoV-2 in COVID-19 patients showed that NAbs seroconversion occurred at around 5.5 days post onset, with a positivity rate of 52% within the first week, reaching 100% by the third week, and maintaining above 97% for up to 6 months. NAbs peaked in the fourth week and were positively associated with disease severity and age, while inversely associated with serum albumin levels. The half-life of NAbs was 61 days within the first two months, which then slowed to 104 days afterward.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2021)
Article
Immunology
Yang Li, Dan-yun Lai, Qing Lei, Zhao-wei Xu, Feng Wang, Hongyan Hou, Lingyun Chen, Jiaoxiang Wu, Yan Ren, Ming-liang Ma, Bo Zhang, Hong Chen, Caizheng Yu, Jun-biao Xue, Yun-xiao Zheng, Xue-ning Wang, He-wei Jiang, Hai-nan Zhang, Huan Qi, Shu-juan Guo, Yandi Zhang, Xiaosong Lin, Zongjie Yao, Pengfei Pang, Dawei Shi, Wei Wang, Xiao Yang, Jie Zhou, Huiming Sheng, Ziyong Sun, Hong Shan, Xionglin Fan, Sheng-ce Tao
Summary: Serological tests are crucial in the fight against the COVID-19 pandemic. A peptide microarray analysis identified several high-performing 12-mer peptides derived from the S protein for accurate diagnosis of COVID-19 cases, with one peptide showing comparable sensitivity and specificity to the S1 protein in monitoring IgG response.
CELLULAR & MOLECULAR IMMUNOLOGY
(2021)
Article
Immunology
Sachie Nakagama, Yu Nakagama, Yuko Komase, Masaharu Kudo, Takumi Imai, Evariste Tshibangu-Kabamba, Yuko Nitahara, Natsuko Kaku, Yasutoshi Kido
Summary: More people with a history of prior infection are receiving SARS-CoV-2 vaccines. Understanding the level of protection granted by 'hybrid immunity', the combined response of infection- and vaccine-induced immunity, may impact vaccination strategies through tailored dosing. Analysis of participants with prior infection showed higher antibody titers and enhanced immune response, especially among older individuals. These findings contribute to the growing body of evidence supporting the effectiveness of booster vaccinations following prior infection.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Daniel M. Altmann, Catherine J. Reynolds, George Joy, Ashley D. Otter, Joseph M. Gibbons, Corinna Pade, Leo Swadling, Mala K. Maini, Tim Brooks, Amanda Semper, Aine Mcknight, Mahdad Noursadeghi, Charlotte Manisty, Thomas A. Treibel, James C. Moon, Rosemary J. Boyton
Summary: This study analyzes the immune response in healthcare workers with mild or asymptomatic SARS-CoV-2 infection and compares it to those with Long Covid. The results show no significant differences in immune responses between the two groups up to 18 weeks post-infection and 1 year after vaccination, suggesting that immune response variations are unlikely to be a contributing factor to Long Covid development.
NATURE COMMUNICATIONS
(2023)
Article
Biophysics
Riccardo Funari, Hidehiro Fukuyama, Amy Q. Shen
Summary: A multiplex nanoplasmonic biosensor has been developed to capture the humoral response against multiple antigens, providing a cost-effective and efficient alternative for serological analysis. The platform has demonstrated successful profiling of multiple serum antibodies and can be integrated with microfluidics for high throughput screening in vaccine development.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Polymer Science
Vincenzo Calabrese, Stylianos Varchanis, Simon J. Haward, Amy Q. . Shen
Summary: Understanding the hydrodynamic alignment of colloidal rods in polymer solutions is crucial for producing structurally ordered materials. This study analyzes the alignment of colloidal rods suspended in polymer solutions and compares it to the case where additional colloidal rods provide crowding in a pure solvent. The dynamics of the polymer is found to govern the shear-induced alignment of colloidal rods, with the Weissenberg number as the control parameter for alignment. Additionally, it is shown that the alignment of colloidal rods follows a universal trend regardless of the crowding environment.
Article
Chemistry, Physical
Rameez Iqbal, Atsushi Matsumoto, Daniel Carlson, Kazumi Toda Peters, Riccardo Funari, Ashis K. Sen, Amy Q. Shen
Summary: In this study, a composite rigid-soft PDMS substrate was used to achieve receding and deposition of microparticles on the surface of a droplet. By adjusting the ratio of the rigid-soft regions, different sizes and shapes of deposition areas could be obtained. Compared to traditional lithography methods, this approach using 3D printing and soft lithography was simpler and provided a new avenue for patterning.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Mechanics
Daniel W. Carlson, Kazumi Toda-Peters, Amy Q. Shen, Simon J. Haward
Summary: This study investigates the phenomenon of elastic turbulence and its effects on the flow state of polymer chains and flow fields. By conducting experimental observations and analysis on the flow in a three-dimensional ordered porous medium, it is found that elastic turbulence can be triggered when specific parameters exceed a critical value. In the state of elastic turbulence, polymer chains are influenced by the curvature of the flow streamline, leading to flow instability.
JOURNAL OF FLUID MECHANICS
(2022)
Letter
Mechanics
San To Chan, Simon J. Haward, Amy Q. Shen
Summary: Edge fracture, a common problem in measuring rheological properties of viscoelastic fluids at high shear rates, can be delayed by sealing the fluid's free surface with nontoxic liquid metal Galinstan. This approach extends the measurable shear rate range of a cone-and-plate fixture significantly, compared to the existing method of using a cone-partitioned plate fixture. The inexpensive yet effective method proposed in this study will benefit the general rheological study of complex fluids.
Article
Biochemical Research Methods
Vincenzo Calabrese, Amy Q. Q. Shen, Simon J. J. Haward
Summary: This article analyzes the structural dynamics of naturally derived colloidal rods (CR) under microfluidic flows, focusing on shear- and extensional-dominated flows. It highlights the importance of understanding key concepts when evaluating results from different CR and microfluidic platforms, and suggests future research directions.
Article
Chemistry, Multidisciplinary
Vincenzo Mazzaracchio, Mauricio Rios Maciel, Tatiana Porto Santos, Kazumi Toda-Peters, Amy Q. Shen
Summary: A duplex electrochemical immunosensor is developed for simultaneous detection of anti-nucleocapsid IgG (N-IgG) and anti-spike IgG (S-IgG) using a two-working electrode system and differential pulse voltammetry. The sensor exhibits enhanced performance by modifying the screen-printed electrodes with carbon black and electrodeposited gold nanoflowers. It achieves a wide linear range and low detection limits for N-IgG and S-IgG detection in serum samples. Integrated into a microfluidic device, the duplex immunosensor enables high throughput screening for COVID-19 and other infectious diseases with significantly reduced detection time.
Review
Physics, Fluids & Plasmas
Charlotte de Blois, Simon J. Haward, Amy Q. Shen
Summary: In the flow of viscoelastic fluids over a microfluidic canopy, waves in the form of low flow velocity regions compared to the surrounding flow spontaneously emerge. The occurrence of these waves is chaotic and exhibits characteristics of elastic turbulence. By combining flow velocimetry experiments and high-speed tracking of the pillars, we systematically study the coupling between the low-velocity waves and the microfluidic canopy.
PHYSICAL REVIEW FLUIDS
(2023)
Article
Biochemistry & Molecular Biology
Marvin Detert, Tatiana Porto Santos, Amy Q. Shen, Vincenzo Calabrese
Summary: The relationship between the alignment of biosourced rod-like colloids (RC) and rod-like polymers (RP) and the rheological properties of the fluid is investigated. It is found that the alignment of RC and RP contributes to the viscosity of the fluid in a consistent manner across different systems, independent of concentration. A dimensionless parameter (& beta;) directly proportional to the specific viscosity (& eta;(sp)) at zero shear rate (& eta;(0,sp)) is also identified.
Article
Mechanics
Simon J. Haward, Francisco Pimenta, Stylianos Varchanis, Daniel W. Carlson, Kazumi Toda-Peters, Manuel A. Alves, Amy Q. Q. Shen
Summary: Numerical optimization of a six-arm cross-slot device generates three-dimensional fluidic channel shapes that approximate ideal uniaxial (biaxial) stagnation point extensional flow. One of the designed geometries is suitable for microfabrication and achieves optimal flow fields for both Newtonian and viscoelastic fluids. The geometry is called OUBER and is fabricated with high precision by laser-induced etching. Microtomographic-particle image velocimetry measurements confirm accurate imposition of desired flows in a wide region around the stagnation point. In Part II of the paper, pressure drop measurements in the OUBER geometry are used to evaluate extensional rheometry of dilute polymeric solutions compared to planar extension measurements using OSCER.
JOURNAL OF RHEOLOGY
(2023)
Article
Mechanics
Simon J. Haward, Stylianos Varchanis, Gareth H. McKinley, Manuel A. Alves, Amy Q. Shen
Summary: Part I presents a microfluidic device for generating near-homogeneous uniaxial and biaxial elongational flows. In Part II, this device is used to examine the extensional rheology of dilute polymer solutions compared to measurements made under planar extension. The extension rate and tensile stress difference generated in the fluid are measured using micro-particle image velocimetry and excess pressure drop measurements. The results show that at higher polymer concentrations, the experimental measurements deviate from the model predictions due to intermolecular interactions as the polymer chains unravel in the extensional flows.
JOURNAL OF RHEOLOGY
(2023)
Article
Mechanics
San To Chan, Stylianos Varchanis, Simon J. Haward, Amy Q. Shen
Summary: Edge fracture is a viscoelastic instability that occurs when a fluid experiences a high shear rate, causing sudden indentation of its free surface. This fracture can affect rheological measurements and lead to complications in interpreting experimental results. Through empirical and theoretical research, the physics of edge fracture has been unraveled, enabling rheologists to develop techniques for reducing its adverse effects. Furthermore, edge fracture has potential applications in breaking viscoelastic liquid bridges, making it useful for functional dispensing nozzles.
JOURNAL OF RHEOLOGY
(2023)
Article
Mechanics
M. A. Kanso, V. Calabrese, Amy Q. Q. Shen, Myong Chol Pak, A. J. Giacomin
Summary: Bacteriophages are viruses that attack bacteria by attaching to their receptors, and their reorientation relies on Brownian motion and rotational components. In this study, the rotational diffusivity of Pf1, a type of bacteriophage, was determined through rheological measurements and compared with theoretical predictions. The results showed that the dimensionless rotational diffusivity differed by approximately one order of magnitude from the value obtained from fluorescence microscopy. Good agreement was found between theoretical predictions and complex viscosity measurements at low frequency.
Article
Chemistry, Physical
Arisa Yokokoji, Stylianos Varchanis, Amy Q. Shen, Simon J. Haward
Summary: Viscoelastic flows in the cross-slot geometry can transition from a steady symmetric state to a steady asymmetric state. This transition may be due to purely-elastic effects beyond a critical flow rate, or shear thinning of the fluid's viscosity. The interplay between fluid elasticity and shear thinning affects the onset and development of asymmetric flows. Experimental results and numerical simulations reveal the importance of shear thinning and fluid elasticity in controlling elastic instabilities in the cross-slot geometry.
Article
Multidisciplinary Sciences
San To Chan, Stylianos Varchanis, Amy Q. Shen, Simon J. Haward
Summary: This study investigates the possibility of using torsion to break liquid bridges of complex thixotropic elastoviscoplastic fluids and demonstrates its potential application in improving the dispensing of real industrial fluids. Through simulation and dimensional analysis, a better understanding of the liquid bridge breaking mechanism is achieved.
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)