Article
Immunology
Riham Abdel-Hamid Haroun, Waleed H. Osman, Rasha E. Amin, Asmaa M. Eessa, Soha Saad
Summary: This study aimed to compare the diagnostic and prognostic accuracy of various laboratory biomarkers in severe COVID-19 patients. The results showed that levels of neutrophil percentage, CRP, IL-6, PCT, and LDH were significantly increased in non-survivors compared to survivors. IL-6 and LDH were found to be good predictors of mortality among severe COVID-19 patients.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Immunology
Amal A. Gharamti, Fei Mei, Katherine C. Jankousky, Jin Huang, Peter Hyson, Daniel B. Chastain, Jiawei Fan, Sharmon Osae, Wayne W. Zhang, Jose G. Montoya, Kristine M. Erlandson, Sias J. Scherger, Carlos Franco-Paredes, Andres F. Henao-Martinez, Leland Shapiro
Summary: The study indicates that an F/P ratio >= 877 increases the accuracy of diagnosing COVID-19 pneumonia, providing some assistance in distinguishing COVID-19 from bacterial pneumonia.
OPEN FORUM INFECTIOUS DISEASES
(2021)
Article
Medicine, General & Internal
Eun-Hwa Lee, Kyoung-Hwa Lee, Young-Goo Song, Sang-Hoon Han
Summary: C-reactive protein (CRP) or procalcitonin (PCT) alone have limitations in the early detection of infection or inflammation. Interleukin (IL)-6 has been assessed recently, and this study reveals considerable discrepancies among CRP, PCT, and IL-6 levels. Relying solely on CRP tests may lead to misinterpretation.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Medicine, General & Internal
Angela Quirino, Nadia Marascio, Cinzia Peronace, Giorgio S. Barreca, Luigia Gallo, Aida Giancotti, Angelo G. Lamberti, Enrico M. Trecarichi, Carlo Torti, Maria Mazzitelli, Martina Bonofiglio, Francesca Divenuto, Giovanni Matera, Maria C. Liberto
Summary: This study aims to assess the relationship between genes positive for non-specific pro-inflammatory markers C-reactive protein (CRP) and procalcitonin (PCT), as well as specific diagnostic and prognostic tools IgG and IgM, in SARS-CoV-2 RT-PCR positive patients. The significant increase of IgG and IgM in deceased patients is a key finding of this study. The evaluation of antibody kinetics and non-specific inflammatory markers can aid in the diagnosis and prognosis of COVID-19 patients.
Article
Biotechnology & Applied Microbiology
Jason Z. Zhang, Hsien-Wei Yeh, Alexandra C. Walls, Basile I. M. Wicky, Kaitlin R. Sprouse, Laura A. VanBlargan, Rebecca Treger, Alfredo Quijano-Rubio, Minh N. Pham, John C. Kraft, Ian C. Haydon, Wei Yang, Michelle DeWitt, John E. Bowen, Cameron M. Chow, Lauren Carter, Rashmi Ravichandran, Mark H. Wener, Lance Stewart, David Veesler, Michael S. Diamond, Alexander L. Greninger, David M. Koelle, David Baker
Summary: We developed a protein biosensor that can rapidly and sensitively detect neutralizing antibodies against SARS-CoV-2 variants in serum. The biosensor can accurately differentiate between different samples based on the affinity and abundance of antibody binding, which is superior to traditional competition-based assays.
NATURE BIOTECHNOLOGY
(2022)
Article
Immunology
Vikram Jain, Pratap Kumar, Prasan Kumar Panda, Mohan Suresh, Karanvir Kaushal, Anissa A. Mirza, Rohit Raina, Sarama Saha, Balram J. Omar, Vivekanandhan Subbiah
Summary: This study investigated the role of IL-6 in COVID-19 diagnosis, treatment, and prognosis, suggesting that IL-6 levels above 40 pg/mL are associated with higher rates of mortality, ICU admissions, and ventilator requirements.
Article
Cardiac & Cardiovascular Systems
Anu Kataja, Tuukka Tarvasmaki, Johan Lassus, Alessandro Sionis, Alexandre Mebazaa, Kari Pulkki, Marek Banaszewski, Valentina Carubelli, Mari Hongisto, Ewa Jankowska, Raija Jurkko, Toni Jantti, Monika Kasztura, John Parissis, Tuija Sabell, Jose Silva-Cardoso, Jindrich Spinar, Heli Tolppanen, Veli-Pekka Harjola
Summary: Inflammatory markers, particularly PCT and IL-6, are strongly associated with signs of systemic hypoperfusion in cardiogenic shock (CS) and predict poor prognosis. High levels of PCT and IL-6 are linked to increased 90-day mortality rates, while CRP levels do not show significant prognostic value.
INTERNATIONAL JOURNAL OF CARDIOLOGY
(2021)
Article
Immunology
Alberto Ponciano-Gomez, Martha Iris Valle-Solis, Myriam Campos-Aguilar, Rafael Jijon-Lorenzo, Elena De La C. Herrera-Cogco, Roberto Ramos-Alor, Cesar Isaac Bazan-Mendez, Gustavo Antonio Perez-Gil Cervantes, Ricardo Avila-Garcia, Abdiel Gonzalez Aguilar, Moises Geovani Salmeron Texale, Wilfrido David Tapia-Sanchez, Carlos Leonardo Duarte-Martinez, Sandra Olivas-Quintero, Santiago Cristobal Sigrist-Flores, Itzell Alejandrina Gallardo-Ortiz, Rafael Villalobos-Molina, Adolfo Rene Mendez-Cruz, Rafael Jimenez-Flores, Leopoldo Santos-Argumedo, Juan Pedro Luna-Arias, Hector Romero-Ramirez, Victor Hugo Rosales-Garcia, Bartolo Avendano-Borromeo
Summary: This study analyzed the immune response of individuals with previous SARS-CoV-2 infection after vaccination. The results showed that individuals with earlier infection had higher antibody concentrations, which could be attributed to increased production of plasma cells and reduced presence of memory B cells in the bloodstream. This could be related to the high baseline expression of cytokines (IL-6 and IL-10) before vaccination.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Lubei Rao, Kaijiong Zhang, Huaichao Luo, Shuya He, Yuping Li, Chang Liu, Ying Yang, Dongsheng Wang
Summary: Infection is a major cause of death in cancer patients, and accurately identifying fever caused by infection is important for avoiding unnecessary treatment. This study evaluated the diagnostic value of various inflammatory markers in suspected infected cancer patients, and found that procalcitonin had the best performance in differentiating infected from uninfected causes of fever.
IMMUNOLOGIC RESEARCH
(2022)
Article
Immunology
Emanuel Della-Torre, Marco Lanzillotta, Corrado Campochiaro, Giulio Cavalli, Giacomo De Luca, Alessandro Tomelleri, Nicola Boffini, Rebecca De Lorenzo, Annalisa Ruggeri, Patrizia Rovere-Querini, Antonella Castagna, Giovanni Landoni, Moreno Tresoldi, Fabio Ciceri, Alberto Zangrillo, Lorenzo Dagna
Summary: This study shows that IL-1 and IL-6 blockade therapy is more likely to provide a survival advantage in hyper-inflamed COVID-19 patients, especially when initiated before the establishment of severe respiratory failure.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Engineering, Multidisciplinary
Madeshwari Ezhilan, Indhu Suresh, Noel Nesakumar
Summary: Highly pathogenic MERS-CoV, SARS-CoV, and SARS-CoV-2 viruses cause acute respiratory syndrome and present major challenges to global health, especially in terms of infection control and public health services. Accurate assessment and diagnostic methods are crucial for clinical guidance and evaluating outcomes. This review highlights the history, detection methods, safety guidelines, and legal regulations related to these viruses, while also discussing the challenges in clinical diagnosis and the importance of developing alternative diagnostic platforms.
Article
Biotechnology & Applied Microbiology
Jason Z. Zhang, Hsien-Wei Yeh, Alexandra C. Walls, Basile I. M. Wicky, Kaitlin R. Sprouse, Laura A. VanBlargan, Rebecca Treger, Alfredo Quijano-Rubio, Minh N. Pham, John C. Kraft, Ian C. Haydon, Wei Yang, Michelle DeWitt, John E. Bowen, Cameron M. Chow, Lauren Carter, Rashmi Ravichandran, Mark H. Wener, Lance Stewart, David Veesler, Michael S. Diamond, Alexander L. Greninger, David M. Koelle, David Baker
Summary: The protein biosensor designed in this study utilizes thermodynamic coupling to detect neutralizing antibodies against SARS-CoV-2 variants, without the need for target modification, and can better distinguish differences between samples compared to traditional methods.
NATURE BIOTECHNOLOGY
(2022)
Article
Medicine, General & Internal
Robert Flisiak, Jerzy Jaroszewicz, Magdalena Rogalska, Tadeusz Lapinski, Aleksandra Berkan-Kawinska, Beata Bolewska, Magdalena Tudrujek-Zdunek, Dorota Kozielewicz, Marta Rorat, Piotr Leszczynski, Krzysztof Klos, Justyna Kowalska, Pawel Pabjan, Anna Piekarska, Iwona Mozer-Lisewska, Krzysztof Tomasiewicz, Malgorzata Pawlowska, Krzysztof Simon, Joanna Polanska, Dorota Zarebska-Michaluk
Summary: The study demonstrated the significant impact of tocilizumab on reducing mortality and accelerating clinical improvement in COVID-19 patients with a baseline concentration of IL-6 > 100 pg/mL, particularly those who require oxygen supplementation.
JOURNAL OF CLINICAL MEDICINE
(2021)
Review
Public, Environmental & Occupational Health
Rizaldy Taslim Pinzon, Vincent Ongko Wijaya, Ranbebasa Bijak Buana
Summary: The systematic review and meta-analysis showed that IL-6 inhibitor agents can significantly reduce mortality in COVID-19 patients, especially in severe cases, while no significant differences were found in other outcomes compared to controls. Further well-designed trials are needed to evaluate the efficacy and safety of IL-6 inhibitor agents for COVID-19.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2021)
Article
Infectious Diseases
Niklas Broman, Thijs Feuth, Tytti Vuorinen, Mika Valtonen, Ulla Hohenthal, Eliisa Loyttyniemi, Tiina Hirvioja, Paivi Jalava-Karvinen, Harri Marttila, Marika Nordberg, Jarmo Oksi
Summary: Severe COVID-19 patients with enhanced inflammation showed improved clinical recovery and shorter hospitalization when treated with tocilizumab compared to standard of care alone, according to this study.
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)