期刊
BIORESOURCE TECHNOLOGY
卷 318, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biortech.2020.124217
关键词
LPMO10; Myxobacteria; Agricultural residual biomass; Cellulase cocktail
资金
- Guangdong Province Science and Technology Innovation Strategy Special Fund [2018B020206001]
- Science and Technology Plan Project of Guangdong Province [2016A010105013]
- GDAS' Special Project of Science and Technology Development [2020GDASYL-20200302002]
- National Natural Science Foundation of China [31400681]
Myxobacteria are soil microorganisms with the ability to break down biological macromolecules due to the secretion of a large number of extracellular enzymes, but there has been no research report on myxobacterial lytic polysaccharide monooxygenases (LPMOs). In this study, two LPMO10s, ViLPMO10A and ViLPMO10B, from myxobacterium Vitiosangium sp. GDMCC 1.1324 were characterized. Of which, ViLPMO10B is a C1-oxidizing cellulose-active LPMO. Moreover, ViLPMO10B could decrease the degree of polymerization of crop straws cellulose and synergize with commercial cellulase to promote the saccharification. When the weight ratio of commercial cellulase to ViLPMO10B was 9:1, the conversion efficiency of corn stalk, sugarcane bagasse, and rice straw into reducing sugar was improved by 17%, 16%, and 22%, respectively, compared with commercial cellulase without ViLPMO10B. These results indicate that ViLPMO10B has the potential to be a component of a high-efficient cellulase cocktail and has application value in the saccharification of agricultural residual biomasses.
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