期刊
BIOMEDICAL CHROMATOGRAPHY
卷 35, 期 2, 页码 -出版社
WILEY
DOI: 10.1002/bmc.4985
关键词
gut microbial metabolites; liquid chromatography– tandem mass spectrometry; quantitative assay; type 2 diabetes mellitus
类别
资金
- National Natural Science Foundation of China [U1803123, U1903211]
- Zhongshan Science and Technology Program [2016C1015]
- Science Program for Overseas Scholar of Guangzhou University of Chinese Medicine (Torch Program) [XH20170111]
- Guangdong Provincial Key Laboratory of Construction Foundation [2017B030314030]
- Science Program for Overseas Scholar of Guangzhou University of Chinese Medicine [XH20170111]
A rapid and sensitive assay based on LC-tandem mass spectrometry was developed to analyze 20 gut microbial metabolites in rat serum, urine, and faeces. The method exhibited good linear relationships, precision values below 15%, and accuracies ranging from 85% to 115%. Successful application of the method in characterizing alterations of metabolites in type 2 diabetes mellitus rat highlights its potential for high-throughput monitoring of gut microbial metabolites in diseases.
Gut microbial phenylalanine, tyrosine, and tryptophan metabolites are closely linked to various diseases. Monitoring the alterations of the related metabolites is vital to facilitate the understanding of pathophysiology of diseases. Herein, a rapid and sensitive assay based on LC-tandem mass spectrometry has been developed to analyze 20 gut microbial metabolites derived from phenylalanine, tyrosine, and tryptophan in rat serum, urine, and faeces. These microbial-derived metabolites were separated on a phenyl-hexyl column and simultaneously determined in a single run of 8 min. The detection limit for analytes ranged between 1.08 and 32.4 ng/mL. All calibration curves exhibited good linear relationships (R-2 >= 0.9982). Intra- and inter-assay precision values were below 15% and accuracies ranged from 85% to 115% for all analytes. The selectivity, matrix effect, and recovery of this method were all satisfactory. The validated method was successfully applied to characterize the alterations of these metabolites in type 2 diabetes mellitus rat. In general, the developed assay is suitable for high-throughput monitoring of gut microbial phenylalanine, tyrosine, and tryptophan metabolites and provides a useful approach for exploring the mechanisms of microbial-derived metabolites in diseases.
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