Article
Engineering, Chemical
Jie Gao, Kwee Hiang Jackson Low, Yang Chen, E. Shyong Tai, Tai-Shung Chung, Chester Lee Drum
Summary: This study presents a novel approach using electrospun polyacrylonitrile membranes to separate red blood cells in blood and collect and stabilize plasma biomarkers while retaining human serum albumin. The physicochemical properties of the separation membranes can be further optimized for potential applications in microvolume sample collection.
Article
Chemistry, Analytical
Amirhossein Mehran, Peyman Rostami, Mohammad Said Saidi, Bahar Firoozabadi, Navid Kashaninejad
Summary: The study introduces a passive, label-free microfluidic device with a unique U-shaped cross-section for rapid and high-efficiency isolation of white blood cells (WBCs) from whole blood. This method demonstrates high throughput, purity, and capacity for WBC isolation without the need for centrifugation, RBC lysis, cell biomarkers, or chemical pre-treatments, making it ideal for downstream cell study platforms.
Article
Chemistry, Analytical
Zhiqing Xiao, Lexin Sun, Yuqian Yang, Zitao Feng, Sihan Dai, Hao Yang, Xingwei Zhang, Chia-Lin Sheu, Weijin Guo
Summary: The study demonstrated a passive microfluidic device for efficient plasma separation using a combination of blood filtration membrane and off-stoichiometry thiol-ene (OSTE) pillar forest technology. The device can process whole blood samples quickly, achieving a plasma separation yield as high as 60.0% and a protein recovery rate of 85.5%.
Article
Chemistry, Analytical
Somayyeh Bakhtiaridoost, Hamidreza Habibiyan, Hassan Ghafoorifard
Summary: This study presents a fast, hemolysis-free, highly efficient blood plasma separation microfluidic device that combines gravitational sedimentation with dielectrophoresis force. Using finite element analysis, the effect of geometrical parameters on the separation process is investigated to achieve optimal design specifications. The device achieves a purity of 99.98% and a relative increase in flow rate compared to previous studies by utilizing a porous Melt-Blown Polypropylene layer and optimizing the dielectrophoresis force.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Farshad Ardakani, Bahram Hemmateenejad
Summary: In this study, a new type of microfluidic paper-based analytical device (μPAD) was developed for the separation of plasma from whole blood without using plasma separation membrane. By controlling the pore size of the paper through lamination and electrolyte addition, the device achieved a longer separation distance for plasma with no significant hemolysis. The device has the potential for multi-assay testing in point-of-care experiments.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemical Research Methods
Gayan C. Bandara, Linus J. Unitan, Matthew H. Kremer, Owen T. Shellhammer, Shay Bracha, Vincent T. Remcho
Summary: Traditional blood separation methods require complex equipment and large sample volumes, but with the development of microfluidic devices, blood separation and analysis can now be achieved effectively in small sample sizes. By utilizing a simple fabrication process, these devices can separate blood and integrate colorimetric assay chemistries for semi-quantitative detection in microscale samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Han Zhang, Kanjirakat Anoop, Can Huang, Reza Sadr, Rohit Gupte, Jing Dai, Arum Han
Summary: This study introduces a continuous-flow blood plasma separation microfluidic device based on size exclusion cell separation. The device improves the efficiency, hemolysis resistance, and microchannel clogging compared to conventional designs, through the use of optimized crossflow microchannels and a circular geometry. The device achieves higher separation efficiency and produces high-quality blood plasma.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Fisheries
Zubyda Mushtari Nadia, Aklima Rahman Akhi, Prosun Roy, Farhabun Binte Farhad, M. Mosharraf Hossain, Md. Abdus Salam
Summary: The purpose of this study was to investigate the effect of probiotics on the growth of tilapia and tomato in aquaponics. The results showed that the combination of probiotics and biofloc technology significantly increased the growth of tilapia and tomato production compared to other treatments, and also improved the water quality.
AQUACULTURE REPORTS
(2023)
Article
Engineering, Chemical
Antoine Venault, Yi-Tung Chin, Irish Maggay, Chih-Chen Yeh, Yung Chang
Summary: This work investigates the association of P(S-r-AA) copolymer with PVDF in the fabrication of electrospun fibers for blood filtration, showing improved wetting of nanofibers and enhanced separation and retention of white blood cells. The results demonstrate the potential of PVDF-based nanofibers as efficient separation media for whole blood, with further efforts needed to enhance biocompatibility and red blood cell recovery.
JOURNAL OF MEMBRANE SCIENCE
(2022)
Article
Biochemical Research Methods
Shadi Karimi, Mohammad Mojaddam, Sahand Majidi, Pouya Mehrdel, Josep Farre-Llados, Jasmina Casals-Terre
Summary: This study focuses on optimizing the design of a microfluidic blood plasma separator for portable blood plasma separation with high purity and reasonable yield. The experimental results show that the design can achieve a 19% yield with 77.1% purity, which can be adjusted based on the point-of-care application requirements.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Zaidon T. Al-aqbi, Salim Albukhaty, Ameerah M. Zarzoor, Ghassan M. Sulaiman, Khalil A. A. Khalil, Tareg Belali, Mohamed T. A. Soliman
Summary: A novel microfluidic device featuring a nano-junction was developed for on-chip filtration and out-chip collection of blood plasma with a high extraction yield; the device effectively removes blood cells and plasma proteins, allowing for the detection of analytes at low concentrations.
Article
Polymer Science
Sandra Garcia-Rey, Jacob B. Nielsen, Gregory P. Nordin, Adam T. Woolley, Lourdes Basabe-Desmonts, Fernando Benito-Lopez
Summary: Additive manufacturing technology is an emerging method for rapid prototyping that allows for the creation of complex geometries through layer-by-layer fabrication. This research utilized a custom-made resin formulation and high-resolution 3D printing to optimize a prototype for an operational plasma separation modular device.
Article
Nanoscience & Nanotechnology
Ashok Kumar Loganathan, Ramya Devaraj, Lalithambigai Krishnamoorthy
Summary: This research developed a structural design for microfluidic channels and investigated the impact of different channel designs on blood plasma separation. The results showed that the channel model had little effect on cell displacement or isolation at low flow rates, making it difficult for blood separation. However, an optimized design with changes in angle and serpentine width was able to obtain more plasma with higher purity.
MICROFLUIDICS AND NANOFLUIDICS
(2023)
Article
Multidisciplinary Sciences
Eleanor Brindle, Lorraine Lillis, Rebecca Barney, Pooja Bansil, Francisco Arredondo, Neal E. Craft, Eileen Murphy, David S. Boyle
Summary: Processing and storing blood samples can be challenging in resource limited environments. Dried blood spots (DBS) from finger-stick collection of whole blood have been widely used for this purpose. A simple blood fractionation tool has been evaluated in this study to passively separate plasma from other blood components. The results show that this tool allows for the simultaneous measurement of seven protein biomarkers related to micronutrient deficiencies, inflammation, and malarial antigenemia.
Article
Chemistry, Multidisciplinary
Seyong Kwon, Jieung Oh, Min Seok Lee, Eujin Um, Joonwoo Jeong, Joo H. Kang
Summary: A hemolysis-free and highly efficient plasma separation platform has been developed using enhanced diamagnetic repulsion of blood cells. By supplementing blood with superparamagnetic iron oxide nanoparticles, complete removal of blood cells from blood plasma is achieved, allowing for successful collection of plasma without losing plasma proteins, platelets, or exosomes.