Article
Biochemical Research Methods
Sang-Soo Lee, Seil Kim, Hee Min Yoo, Da-Hye Lee, Young-Kyung Bae
Summary: This study reported the production of a lentiviral SARS-CoV-2 reference material, which can be used to evaluate the quality and performance of SARS-CoV-2 molecular testing, providing accurate copy number values to improve the reliability of SARS-CoV-2 molecular testing.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Juan Manuel Carreno, Damodara Rao Mendu, Viviana Simon, Masood A. Shariff, Gagandeep Singh, Vidya Menon, Florian Krammer
Summary: This study analyzed the antibody responses of healthcare workers using different serological tests, finding a good correlation between RBD and spike-based assays. Antibody levels in HCWs declined over time, while the seroprevalence of NP-reactive antibodies significantly decreased.
Article
Medicine, General & Internal
Adam Konka, Mateusz Lejawa, Jadwiga Gazdzicka, Aneta Bochenek, Martyna Fronczek, Joanna Katarzyna Strzelczyk
Summary: This study analyzed epidemiological data from a major laboratory in the Silesian voivodship of Poland and found that SARS-CoV-2 infections are more common in urban areas. There were discrepancies in the detection of SARS-CoV-2 variants in samples from potentially reinfected patients. The study highlights the higher risk of infection in the Upper Silesian population and the potential impact on the economy and healthcare in the region.
Article
Biochemical Research Methods
Chunyan Niu, Xia Wang, Yunhua Gao, Xiaoting Qiao, Jie Xie, Yongzhuo Zhang, Di Wang, Lianhua Dong
Summary: This study established a new method to quantify SARS-CoV-2 RNA without the need for reverse transcription. Compared to traditional methods, this approach offers higher reliability and accuracy.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Medicine, General & Internal
Tingting Du, Zhongliang Wang
Summary: Since 2019, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a global public health event. Real-time reverse transcription polymerase chain reaction (RT-PCR) tests of upper respiratory tract specimens have been commonly used for confirmation of SARS-CoV-2. A retrospective analysis of patients diagnosed with coronavirus disease 2019 in a hospital in Wuhan was conducted, focusing on the pattern of results from repeated RT-PCR tests. Out of 984 hospitalized patients, 36.8% showed positive results from repeated RT-PCR tests. However, consecutive negative RT-PCR tests did not guarantee viral clearance, as some patients tested positive even after multiple negative tests.
Article
Medical Laboratory Technology
Sang-Soo Lee, Jae-Hyeong Park, Young-Kyung Bae
Summary: This study examined the application of digital PCR in nucleic acid quantification. Digital real-time PCR showed similar results to traditional dPCR methods in DNA and RNA quantification, especially exhibiting higher sensitivity and precision for low copy number targets.
CLINICA CHIMICA ACTA
(2021)
Article
Cell Biology
Netta Barak, Roni Ben-Ami, Tal Sido, Amir Perri, Aviad Shtoyer, Mila Rivkin, Tamar Licht, Ayelet Peretz, Judith Magenheim, Irit Fogel, Ayalah Livneh, Yutti Daitch, Esther Oiknine-Djian, Gil Benedek, Yuval Dor, Dana G. Wolf, Moran Yassour
Summary: Pooling multiple swab samples for SARS-CoV-2 testing can save 76% of RNA extraction and RT-PCR tests, with higher efficiency and sensitivity than expected, even in populations with varying prevalence rates.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
News Item
Multidisciplinary Sciences
Giorgia Guglielmi
Summary: Scientists are still debating whether millions of cheap, fast diagnostic kits will help control the pandemic.
Article
Chemistry, Analytical
Xinyu Zhuang, Xiao Lu, Henson L. Lee Yu, I-Ming Hsing
Summary: A unique barcoded primer-assisted sample-specific pooled testing strategy (Uni-Pool) was developed to improve the efficiency of pooled testing during the COVID-19 pandemic. This strategy eliminates the need for retesting and allows for accurate and sensitive detection of positive and negative samples. The method was successfully validated and demonstrated for large-scale screening.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Paolo Poggio, Paola Songia, Chiara Vavassori, Veronica Ricci, Cristina Banfi, Silvia Stella Barbieri, Gloria Garoffolo, Veronika A. Myasoedova, Luca Piacentini, Angela Raucci, Alessandro Scopece, Elena Sommariva, Maria Cristina Vinci, Davide Carcione, Maria Luisa Biondi, Maria Elisabetta Mancini, Alberto Formenti, Daniele Andreini, Emilio M. Assanelli, Piergiuseppe Agostoni, Marina Camera, Gualtiero I. Colombo, Maurizio Pesce
Summary: False negatives remain a challenge in COVID-19 diagnostic testing, but the use of digital PCR can help to address this issue by accurately detecting infections in samples with low viral loads.
SCIENTIFIC REPORTS
(2021)
Article
Biochemical Research Methods
Yi Yang, Xiaoli Feng, Yang Pan, Xia Wang, Tao Peng, Chunyan Niu, Wang Qu, Qingcui Zou, Lianhua Dong, Xinhua Dai, Minghua Li, Xiang Fang
Summary: A culture-free method using reverse transcription digital PCR (RT-dPCR) targeting subgenomic RNA (sgRNA) allows for rapid and accurate quantification of active severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) within 3 hours in a biosafety level 2 laboratory. This method can help identify infectious patients in a timely and effective manner, reducing unnecessary isolation, especially during times of high COVID-19 infection rates and strained medical resources.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Physical
Ayesha Aziz, Muhammad Asif, Ghazala Ashraf, Umer Farooq, Qiaoli Yang, Shenqi Wang
Summary: In the ongoing COVID-19 pandemic, the importance of highly sensitive analytical testing platforms for detecting SARS-CoV-2 RNA and antiviral antibodies is emphasized. Prompt and accurate diagnosis is crucial for limiting the viral spread and effective isolation. Various tests, including nucleic acid and serological tests, as well as biosensing technologies, are discussed for detecting SARS-CoV-2. Early diagnosis based on the detection of reactive oxygen species due to virus-induced dysfunctioning of lung cells is highlighted.
CURRENT OPINION IN COLLOID & INTERFACE SCIENCE
(2021)
Article
Immunology
Eelke Brandsma, Han J. M. P. Verhagen, Thijs J. W. van de Laar, Eric C. J. Claas, Marion Cornelissen, Emile van den Akker
Summary: Research indicates that DETECTR and qRT-PCR show similar sensitivity in detecting SARS-CoV-2, with DETECTR potentially serving as a complementary approach to qRT-PCR. DETECTR also demonstrates 100% specificity and higher analytical sensitivity, making it suitable for clinical point-of-care testing.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biochemistry & Molecular Biology
Ho-Jae Lim, Min-Young Park, Young-Hyun Baek, Hyeon-Seo Lee, Inhee Kim, Youngjin Kwon, Youngshin You, Kyoungwoo Nam, Jae-Hyun Yang, Min-Jin Kim, Nae Yu, Yong-Hak Sohn, Jung-Eun Park, Yong-Jin Yang
Summary: Due to the high transmissibility of SARS-CoV-2 variants, the capacity of rRT-PCR testing systems is limited. RATs can play a significant role in preventing community transmission, but further evaluation is needed. We compared the diagnostic performance of four RAT kits to rRT-PCR using 1503 nasopharyngeal swabs and found that all four kits had similar performance in terms of precision, area under the curve, SARS-CoV-2 variant detection, and non-SARS-CoV-2 specificity. The Acrosis kit showed significantly better positive detection rate compared to the other three kits, making it an effective tool to prevent the spread of SARS-CoV-2 in communities during the current COVID-19 pandemic.
Review
Biochemical Research Methods
Sihong Xu, Weijin Huang, Li Zhang, Juanjuan An, Xiuhua Li, Aijing Song, Jianhui Nie, Chuntao Zhang, Youchun Wang
Article
Immunology
Sihong Xu, Aijing Song, Jianhui Nie, Xiuhua Li, Jingyun Li, Zuoyi Bao, Youchun Wang
AIDS RESEARCH AND HUMAN RETROVIRUSES
(2008)
Article
Virology
Jianhui Nie, Aijing Song, Sihong Xu, Xiuhua Li, Youchun Wang
ARCHIVES OF VIROLOGY
(2010)
Article
Virology
Sihong Xu, Aijing Song, Xiuhua Li, Jianhui Nie, Chuntao Zhang, Youchun Wang
Article
Virology
Sihong Xu, Aijing Song, Xiuhua Li, Jingyun Li, Zuoyi Bao, Panyong Mao, Qing Zhao, Youchun Wang
JOURNAL OF CLINICAL VIROLOGY
(2008)
Article
Virology
Huihui Chong, Sihong Xu, Chuntao Zhang, Jianhui Nie, Youchun Wang
JOURNAL OF CLINICAL VIROLOGY
(2009)
Article
Virology
Sihong Xu, Aijing Song, Jianhui Nie, Xiuhua Li, Shufang Meng, Chuntao Zhang, Youchun Wang
JOURNAL OF CLINICAL VIROLOGY
(2012)
Article
Genetics & Heredity
Sihong Xu, Jingyun Li, Zuoyi Bao, Hui Xing, Ping Zhong, Hanping Li, Haiwei Zhou, Xinping Li, Sharon Wu, Aijing Song, Xiuhua Li, Jianhui Niel, Youchun Wang
MOLECULAR DIAGNOSIS & THERAPY
(2010)
Article
Genetics & Heredity
Sihong Xu, Aijing Song, Jianhui Nie, Xiuhua Li, Youchun Wang
MOLECULAR DIAGNOSIS & THERAPY
(2010)
Article
Genetics & Heredity
Sihong Xu, Ping Zhong, Jingyun Li, Aijing Song, Hanping Li, Jianhui Nie, Xiuhua Li, Youchun Wang
MOLECULAR DIAGNOSIS & THERAPY
(2011)
Article
Biochemical Research Methods
Chunyu Liu, Xiuhua Li, Chuntao Zhang, Sihong Xu, Yiming Shao, Hui Zhuang, Xiaoyan Che, Yan Qiu, Hongzhang Yin, Defu Li, Youchun Wang
Article
Virology
Youchun Wang, Aijing Song, Sihong Xu, Xiuhua Li, Huihui Chong, Chenyan Zhao, Jianhui Nie, Chuntao Zhang
JOURNAL OF MEDICAL VIROLOGY
(2008)