期刊
ADVANCED FUNCTIONAL MATERIALS
卷 31, 期 44, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.202008326
关键词
cancer; drug delivery; exosome mimetics; iron oxide nanoparticles; magnetic extrusion
类别
资金
- NIH NCI [R21CA253051]
- Breast Cancer Research Foundation
- OFD/BTREC/CTREC Faculty Career Development Fellowship of Boston Children's Hospital
The developed magnetic extrusion method offers a facile and scaled-up approach for preparing exosome mimetics (EMs) with similar properties and functions as native exosomes, overcoming manufacturing challenges. Additionally, engineered EMs demonstrate efficient drug delivery capabilities and tumor growth inhibition, showing promise as a biological nanomedicine for cancer drug delivery.
To date, the scaled-up manufacturing and efficient drug loading of exosomes are two existing challenges limiting the clinical translation of exosome-based drug delivery. Herein, a facile magnetic extrusion method is developed for preparing endosome-derived vesicles, also known as exosome mimetics (EMs), which share the same biological origin and similar morphology, composition, and biofunctions with native exosomes. The high yield and consistency of this magnetic extrusion method help to overcome the manufacturing bottleneck in exosome research. Moreover, the proposed standardized multi-step method readily facilitates the ammonium sulfate gradient approach to actively load chemodrugs such as doxorubicin into EMs. The engineered EMs developed and tested here exhibit comparable drug delivery properties as do native exosomes, and potently inhibit tumor growth by delivering doxorubicin in an orthotopic breast tumor model. These findings demonstrate that EMs can be prepared in a facile and scaled-up manner as a promising biological nanomedicine for cancer drug delivery.
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