期刊
ACTA BIOMATERIALIA
卷 139, 期 -, 页码 179-189出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.actbio.2020.12.033
关键词
Ti (3) C (2) T x MXene; Aerosol jet printing; Human induced pluripotent stem; cell-derived cardiomyocyte; Cardiac patches; Polyethylene glycol
资金
- NIH [1 R01 HL141909-01A1]
- NSF-CAREER Award [1651385]
- National Science Foundation [CMMI-1747685]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1651385] Funding Source: National Science Foundation
A new composite construct has been developed in this study, which can provide both conductive and topographical cues for cardiac tissue engineering applications, potentially for the treatment of myocardial infarction.
Tissue engineered cardiac patches have great potential as a therapeutic treatment for myocardial infarction (MI). However, for successful integration with the native tissue and proper function of the cells comprising the patch, it is crucial for these patches to mimic the ordered structure of the native extracellular matrix and the electroconductivity of the human heart. In this study, a new composite construct that can provide both conductive and topographical cues for human induced pluripotent stem cell derived cardiomyocytes (iCMs) is developed for cardiac tissue engineering applications. The constructs are fabricated by 3D printing conductive titanium carbide (Ti 3 C 2 T x ) MXene in pre-designed patterns on polyethylene glycol (PEG) hydrogels, using aerosol jet printing, at a cell-level resolution and then seeded with iCMs and cultured for one week with no signs of cytotoxicity. The results presented in this work illustrate the vital role of 3D-printed Ti 3 C 2 T x MXene on aligning iCMs with a significant increase in MYH7, SERCA2 , and TNNT2 expressions, and with an improved synchronous beating as well as conduction velocity. This study demonstrates that 3D printed Ti 3 C 2 T x MXene can potentially be used to create physiologically relevant cardiac patches for the treatment of MI.
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