期刊
ACS NANO
卷 15, 期 1, 页码 111-124出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.0c07397
关键词
DNA; chromosome organization; synthetic cells; bottom-up biology; emergent dynamics; DNA-binding proteins; DNA loop extrusion; phase separation; minimal genome
类别
资金
- ERC [883684]
- NWO-OCW
Chromosome structure and dynamics play a crucial role in gene expression and inheritance. Methods such as GenBox provide a way to study chromosomes in vitro, bridging the gap between top-down experiments on live cells and bottom-up single-molecule studies.
Chromosome structure and dynamics are essential for life, as the way that our genomes are spatially organized within cells is crucial for gene expression, differentiation, and genome transfer to daughter cells. There is a wide variety of methods available to study chromosomes, ranging from live-cell studies to single-molecule biophysics, which we briefly review. While these technologies have yielded a wealth of data, such studies still leave a significant gap between top-down experiments on live cells and bottom-up in vitro single-molecule studies of DNA-protein interactions. Here, we introduce genome in a box (GenBox) as an alternative in vitro approach to build and study chromosomes, which bridges this gap. The concept is to assemble a chromosome from the bottom up by taking deproteinated genome-sized DNA isolated from live cells and subsequently add purified DNA-organizing elements, followed by encapsulation in cell-sized containers using microfluidics. Grounded in the rationale of synthetic cell research, the approach would enable to experimentally study emergent effects at the global genome level that arise from the collective action of local DNA-structuring elements. We review the various DNA-structuring elements present in nature, from nucleoid-associated proteins and SMC complexes to phase separation and macromolecular crowders. Finally, we discuss how GenBox can contribute to several open questions on chromosome structure and dynamics.
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