Article
Infectious Diseases
Yun Wu, Mengyuan Jiang, Shaogang Li, Nicholas R. Waterfield, Guowei Yang
Summary: The study established a rapid and accurate Leishmania species identification method using real-time PCR, which can directly use clinical samples for identification, and has important implications for clinical diagnosis and patient management.
INFECTIOUS DISEASES OF POVERTY
(2022)
Article
Biology
Safiyyah Shahimi, Sahilah Abd. Mutalib, Norfadzilah Ismail, Aishah Elias, Haslaniza Hashim, Mohd Izhar Ariff Mohd Kashim
Summary: This study aimed to detect the presence of chicken and porcine DNA in meatballs using species-specific primers for mitochondrial and nuclear DNA. The use of SINE primers in PCR analysis proved to be useful in identifying porcine DNA in heat-treated chicken meatballs.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Eiseul Kim, Seung-Min Yang, Ik-Seon Kim, Hae-Yeong Kim
Summary: This study extracted novel molecular targets for the identification of major Weissella species in food samples using pangenome analysis. The method showed positive results for the target species and negative results for non-target bacteria, and was successfully applied to identify and quantify Weissella communities in fermented foods.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Food Science & Technology
Regina Klapper, Ute Schroder
Summary: Seafood products are vulnerable to food fraud, highlighting the importance of reliable authentication methods. This study developed a multiplex TaqMan real-time PCR assay for rapid identification of commercially important scallop species.
Article
Food Science & Technology
Christian Brenn, Ute Schroeder, Reinhold Hanel, Pedro Martinez Arbizu
Summary: The study presents a TaqManprobe based multiplex real-time PCR screening assay for identification of four commercially relevant crustacean species, showing high specificity and potential for rapid species identification.
Article
Food Science & Technology
Yuri Kim, Hyun-Sung Lee, Kwang-Geun Lee
Summary: In this study, a commercial DNA extraction kit and a real-time PCR kit were used to detect porcine DNA in Korean processed foods. The method was validated for specificity, sensitivity, applicability, and inter-laboratory reproducibility. The results showed that the method was specific and sensitive with no false-positive or false-negative results. It was also applicable to various food matrices and showed consistent results between different laboratories. The study concluded that the real-time PCR is a reliable method to detect porcine DNA in food and can be easily and routinely used in food facilities.
FOOD SCIENCE AND BIOTECHNOLOGY
(2023)
Article
Food Science & Technology
Jinchun Li, Jiapeng Li, Ruixi Liu, Yixuan Wei, Shouwei Wang
Summary: The study developed a hexaplex real-time PCR assay using melting curve analysis to identify eleven meat species simultaneously, showing good specificity of primers and detection limits of 0.01-0.1 ng DNA and 0.1-1% meat mixture. The method can accurately identify different meat species, indicating great application potential.
Article
Food Science & Technology
Lanxin Zhao, Xiaoyan Zhao, E. Hengchao, Tingting Fan, Enjing Tian, Chunling Wang, Yanmei Zhang, Xiaobei Li, Zhiyong Zhao, Changyan Zhou
Summary: A multiplex TaqMan real-time PCR assay was developed in this study for simultaneous detection of multiple highly toxic mushroom species, with the ability to specifically identify two specific highly toxic mushroom species in other mushroom types. The method had high sensitivity and was successfully applied to cooked mushrooms.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
R. R. Vafin, A. G. Galstyan, S. V. Tyulkin, Kh. Kh. Gilmanov, E. A. Yurova, V. K. Semipyatniy, A. V. Bigaeva
Summary: The development of molecular genetic and bioinformatic systems for identifying the species of milk and dairy products is important for controlling turnover of falsified products. The study aims to develop a method using PCR-RFLP analysis to identify species from agricultural ruminant animals. The method includes sample preparation, nucleic acid extraction, PCR-RFLP technique, gel electrophoresis, and result analysis using software and algorithms.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Food Science & Technology
Ane del Rio-Lavin, Elisa Jimenez, Miguel Angel Pardo
Summary: The study developed a real-time PCR assay for the identification of Mytilus spp. mussels in the European market, showing 100% specificity, sensitivity, and precision, and can be easily applied across different platforms without special software requirements.
Article
Food Science & Technology
Nuray Gamze Yoruk
Summary: The study compared the sensitivity of DNA-based real-time polymerase chain reaction (RT-PCR) and protein-based enzyme-linked immunosorbent analysis (ELISA) methods for meat product detection. While both methods showed 100% success rate in detecting beef products, real-time PCR was more accurate in detecting pork products. ELISA was found to be less time-consuming, easier, and more economical compared to real-time PCR.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2021)
Article
Parasitology
Nikol Reslova, Lucie Skorpikova, Iveta Angela Kyrianova, Jaroslav Vadlejch, Johan Hoglund, Philip Skuce, Martin Kasny
Summary: The study developed two multiplex real-time PCR assays for rapid and accurate identification of gastrointestinal nematode species in ruminants, as well as semi-quantitative estimation of egg numbers. This approach showed higher sensitivity and specificity compared to traditional methods, improving diagnostic accuracy and allowing for more precise evaluation of anthelmintic efficacy.
PARASITES & VECTORS
(2021)
Article
Microbiology
Eiseul Kim, Seung-Min Yang, Ik-Seon Kim, So-Yun Lee, Hae-Yeong Kim
Summary: In this study, a real-time PCR method was developed using pangenome analysis to rapidly identify and differentiate Leuconostoc species in food. Pangenome analysis of 130 Leuconostoc genomes revealed species-specific genes that were validated through in silico testing against publicly available genomes. The real-time PCR method showed high sensitivity, specificity, and anti-interference ability for food sample matrices and non-target strains.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Intiaz Amin Chowdhury, Guiping Yan
Summary: A rapid and accurate PCR method was developed for detecting and identifying a new species of root-lesion nematode. Species-specific primers showed high specificity in both conventional and quantitative real-time PCR assays, with high detection sensitivity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Virology
Ellen Kathrin Link, Matthias Eddicks, Liangliang Nan, Mathias Ritzmann, Gerd Sutter, Robert Fux
Summary: Genotyping of PCV2 is important for diagnosis and epidemiological studies. The new qPCR system introduced in this study is ideal for mass testing and can be a valuable complement to PCV2 sequencing, especially in cases of simultaneous infections with multiple PCV2 genotypes.
Article
Biotechnology & Applied Microbiology
Soo Ji Kang, Ji Su Jun, Jeong A. Moon, Kwang Won Hong
Article
Microbiology
Soo-Ji Kang, Min-Joo Kim, Do-Yeong Son, Seok-Seong Kang, Kwang-Won Hong
Summary: This study investigated the genome-wide transcriptional responses of human intestinal epithelial cells to stimulation by CotG-p75 and identified several key genes involved in cell differentiation, development, and regulation of cell proliferation.
Article
Microbiology
Soo Ji Kang, Jeong A. Moon, Do Yeong Son, Kwang Won Hong
Summary: This study constructed spore-displayed p40, a soluble protein derived from Lacticaseibacillus rhamnosus GG, using spore surface display technology. Transcriptome analysis revealed that CotG-p40 had regulatory effects on gene expression, affecting metabolic processes and cell signaling pathways in HT-29 cells. Additionally, 10 genes activated by CotG-p40 were found to be associated with probiotic action.
