Article
Multidisciplinary Sciences
Joseph Puccini, Jia Wei, Liang Tong, Dafna Bar-Sagi
Summary: Macropinocytosis is a nonselective endocytosis process that facilitates the uptake of extracellular fluid-phase cargoes. Tumor cells exploit this process to acquire macromolecules for cell growth and proliferation under nutrient-limiting conditions. This study reveals that the enzyme ATP citrate lyase (ACLY) is a key regulator of macropinocytosis, and it is involved in the actin cytoskeleton. The findings demonstrate a mechanism that links regional acetyl-CoA availability with dynamic protein acetylation, supporting the spatial and temporal modulation of membrane-cytoskeletal interactions required for macropinocytosis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Multidisciplinary Sciences
Gen Honda, Nen Saito, Taihei Fujimori, Hidenori Hashimura, Mitsuru J. Nakamura, Akihiko Nakajima, Satoshi Sawai
Summary: Through quantitative image analysis of Dictyostelium on microfabricated surfaces, it was found that the macropinocytic membrane cup is a distinct mode of topographical guidance driven by the Ras/PI3K/F-actin signaling patch, showing unique responses to micrometer-scale topographical features. This topographical guidance differs from previously known guidance mechanisms that depend on nanometer-scale curvature sensing proteins or stress fibers.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Daria Zdzalik-Bielecka, Agata Poswiata, Kamila Kozik, Kamil Jastrzebski, Kay Oliver Schink, Marta Brewinska-Olchowik, Katarzyna Piwocka, Harald Stenmark, Marta Miaczynska
Summary: The study identified an interactome of AXL and its associations with proteins regulating actin dynamics. GAS6-mediated AXL activation triggered actin remodeling and macropinocytosis, promoting the survival of cancer cells under glutamine-deprived conditions. AXL activation by GAS6 drove invasion of cancer cells through actin-driven cytoskeletal rearrangements.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Angela Ballesteros, Manoj Yadav, Runjia Cui, Kiyoto Kurima, Bechara Kachar
Summary: This study reveals the selective transport of isoforms of protocadherin 15 (PCDH15) by myosins using a heterologous expression system. The results show that MYO7A transports the CD3 isoform, while MYO3A and MYO3B transport the CD2 isoform. In addition, MYO15A has an insignificant role in the transport of PCDH15, and none of the tested myosins transport PCDH15-CD1. The study highlights the importance and complexity of mechanoelectrical transduction (MET) and actin regulation in the formation and maturation of the stereocilia bundle.
SCIENTIFIC REPORTS
(2022)
Review
Cell Biology
Joseph A. Cirilo, Laura K. Gunther, Christopher M. Yengo
Summary: Cytoskeletal motors, particularly myosins, play crucial roles in various mechanical functions in cells, such as muscle contraction and cargo transport. Class III myosins, MYO3A and MYO3B, have been shown to regulate stereocilia lengths in auditory and vestibular inner ear hair cells, with mutations in these myosins leading to hearing loss. Research on these myosins and other stereocilia-associated myosins is important and may lead to novel therapeutic strategies for the treatment of hearing loss.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Anne Houdusse, Margaret A. Titus
Summary: Filopodia, microvilli, and stereocilia are important plasma membrane protrusions supported by actin filaments, playing critical roles in sensing the environment and increasing cell surface area. Actin regulators and specific myosins collaborate to control the length of these structures and transport cargoes along the protrusions. Myosin motors also play surprising roles in the formation and extension of these protrusions.
Article
Cell Biology
Antonino Schepis, Sudhir Kumar, Stefan H. I. Kappe
Summary: Plasmodium sporozoites, the mobile forms of malaria parasites, actively drive the invasion of hepatocytes with the assistance of host cell processes. The initial interactions between sporozoites and hepatocytes induce plasma membrane ruffles and filopodia extensions, which are critical for successful infection. Rho GTPase signaling plays a crucial role in regulating these processes and increasing hepatocyte susceptibility to malaria parasite infection. This study highlights the importance of host cell signaling events in plasma membrane dynamics during malaria parasite invasion of hepatocytes.
Article
Astronomy & Astrophysics
Nandini Sahu, Alister W. Graham, Benjamin L. Davis
Summary: This paper presents the fourth in a series of papers on the black hole mass scaling relations dependent on galaxy morphology and formation. Using a sample of 119 galaxies, the study investigates the correlations between black hole mass and various spheroid parameters. The findings reveal different trends for early- and late-type galaxies, as well as a decrease in scatter as the enclosing aperture increases. The study also provides insights for estimating tidal disruption event rates, binary black hole lifetimes, and gravitational wave characteristic strains.
ASTROPHYSICAL JOURNAL
(2022)
Article
Cell Biology
Shigehiko Yumura, Md Shahabe Uddin Talukder, Mst Shaela Pervin, Md Istiaq Obaidi Tanvir, Takashi Matsumura, Koushiro Fujimoto, Masahito Tanaka, Go Itoh
Summary: This study investigated the mechanism of actin accumulation at the wound site and the involvement of related proteins and signaling pathways in wound repair.
Article
Multidisciplinary Sciences
David Paul, Omer Stern, Yvonne Vallis, Jatinder Dhillon, Andrew Buchanan, Harvey McMahon
Summary: The study reveals an aggregation-dependent endocytosis pathway that facilitates uptake and degradation of protein aggregates. This mechanism of managing exogenous proteotoxicity is crucial for cellular homeostasis. The findings highlight the importance of the response to denaturation of extracellular proteins.
NATURE COMMUNICATIONS
(2023)
Review
Biochemistry & Molecular Biology
Olga Povarova, Iuliia A. Antifeeva, Alexander Fonin, Konstantin K. Turoverov, Irina M. Kuznetsova
Summary: To date, it has been shown that liquid-liquid phase separation (LLPS) plays a role in various cellular processes, providing a new perspective on the spatiotemporal organization of the cell. This new paradigm helps answer long-standing unanswered questions, particularly in the regulation of cytoskeleton assembly/disassembly and actin polymerization. Coacervates of actin-binding proteins formed during LLPS integrate with G-actin to initiate polymerization, while liquid droplet coacervates formed by signaling proteins on the inner side of the cell membrane intensify the activity of actin-binding proteins involved in actin polymerization, such as N-WASP and Arp2/3.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Minhyeok Chang, O-Chul Lee, Gayun Bu, Jaeho Oh, Na-Oh Yunn, Sung Ho Ryu, Hyung-Bae Kwon, Anatoly B. Kolomeisky, Sang-Hee Shim, Junsang Doh, Jae-Hyung Jeon, Jong-Bong Lee
Summary: The study discovered that membrane nanotubes are formed from double filopodial bridges through physical contact and helical deformation. The transition of a double filopodial bridge to a closed-end membrane nanotube is likely triggered by the disruption of adhesion between two filopodia due to accumulated mechanical energy in the twisted double filopodial bridge.
Article
Biochemistry & Molecular Biology
Keqiang Liu, Lina Lu, Shanshan Chen, Beilin Gu, Hui Cai, Ying Wang, Wei Cai
Summary: This study describes a male infant with LMOD1 mutations presenting pediatric intestinal pseudo-obstruction (PIPO) symptoms but without megacystis and microcolon. The mutations result in significantly reduced LMOD1 protein levels and impair LMOD1-mediated actin nucleation. Molecular modeling shows the structural changes caused by the mutations. These findings provide further evidence of LMOD1 as a pathogenic gene and highlight the critical role of the ABS2 domain in LMOD1-mediated actin nucleation and protein stability.
Article
Reproductive Biology
Huizhen Wang, Xiaoman Hong, William H. Kinsey
Summary: Sperm-oocyte binding activates the protein kinase PTK2B in mouse oocytes, promoting actin remodeling, with CD9 playing a significant role in this process.
BIOLOGY OF REPRODUCTION
(2021)
Article
Multidisciplinary Sciences
Sheng Yang, Yubo Tang, Yijun Liu, Abbigale J. Brown, Matthias Schaks, Bojian Ding, Daniel A. Kramer, Magdalena Mietkowska, Li Ding, Olga Alekhina, Daniel D. Billadeau, Saikat Chowdhury, Junmei Wang, Klemens Rottner, Baoyu Chen
Summary: This study reveals a new mechanism for WRC sensing Arf signaling and provides a mechanistic foundation for understanding how WRC-mediated actin polymerization links Arf and Rac signaling in cells.
Article
Oncology
Susan F. Murphy, Robin T. Varghese, Samy Lamouille, Sujuan Guo, Kevin J. Pridham, Pratik Kanabur, Alyssa M. Osimani, Shaan Sharma, Jane Jourdan, Cara M. Rodgers, Gary R. Simonds, Robert G. Gourdie, Zhi Sheng
Article
Biochemistry & Molecular Biology
Xiong Liu, Shi Shu, Neil Billington, Chad D. Williamson, Shuhua Yu, Hanna Brzeska, Julie G. Donaldson, James R. Sellers, Edward D. Korn
JOURNAL OF BIOLOGICAL CHEMISTRY
(2016)
Article
Oncology
Kevin J. Pridham, Lamvy Le, Sujuan Guo, Robin T. Varghese, Sarah Algino, Yanping Liang, Renee Fajardin, Cara M. Rodgers, Gary R. Simonds, Deborah F. Kelly, Zhi Sheng
Article
Multidisciplinary Sciences
Xiong Liu, Neil Billington, Shi Shu, Shu-Hua Yu, Grzegorz Piszczek, James R. Sellers, Edward D. Korn
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Review
Oncology
Kevin J. Pridham, Robin T. Varghese, Zhi Sheng
FRONTIERS IN ONCOLOGY
(2017)
Article
Multidisciplinary Sciences
Sujuan Guo, Kevin J. Pridham, Ching-Man Virbasius, Bin He, Liqing Zhang, Hanne Varmark, Michael R. Green, Zhi Sheng
SCIENTIFIC REPORTS
(2018)
Article
Multidisciplinary Sciences
Robin T. Varghese, Sarah Young, Lily Pham, Yanping Liang, Kevin J. Pridham, Sujuan Guo, Susan Murphy, Deborah F. Kelly, Zhi Sheng
SCIENTIFIC REPORTS
(2018)
Article
Genetics & Heredity
Yanping Liang, William J. Dearnaley, Nick A. Alden, Maria J. Solares, Brian L. Gilmore, Kevin J. Pridham, A. Cameron Varano, Zhi Sheng, Elizabeth Alli, Deborah F. Kelly
Article
Oncology
Kevin L. Sheng, Kevin J. Pridham, Zhi Sheng, Samy Lamouille, Robin T. Varghese
FRONTIERS IN ONCOLOGY
(2019)
Article
Multidisciplinary Sciences
Kevin L. Sheng, Lin Kang, Kevin J. Pridham, Logan E. Dunkenberger, Zhi Sheng, Robin T. Varghese
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Vera Cherkasova, James R. Iben, Kevin J. Pridham, Alan C. Kessler, Richard J. Maraia
Summary: The deletion of the sla1 gene in Schizosachharoymces pombe affects tRNA processing and sensitivity to TOR inhibition. Transcriptome analysis reveals overlap between upregulated genes in sla1 Delta cells and general amino acid control genes. Furthermore, growth in NH4(+) media induces a core environmental stress response in sla1 Delta cells.
Article
Oncology
Kevin J. Pridham, Farah Shah, Kevin L. Sheng, Sujuan Guo, Min Liu, Pratik Kanabur, Samy Lamouille, Gabrielle Lewis, Marc Morales, Jane Jourdan, Christina L. Grek, Gautam G. Ghatnekar, Robin Varghese, Deborah F. Kelly, Robert G. Gourdie, Zhi Sheng
Summary: This study reveals that the protein connexin 43 (Cx43) is highly expressed in a subpopulation of glioblastoma and is associated with poor prognosis and chemoresistance. Targeting Cx43 can sensitize glioblastoma cells to chemotherapy by inactivating phosphatidylinositol-3 kinase (PI3K). Furthermore, combining Cx43 inhibition with PIK3CB/p110 beta-selective inhibitors can synergistically overcome chemoresistance in glioblastoma cells.
Review
Cardiac & Cardiovascular Systems
Spencer R. Marsh, Zachary J. Williams, Kevin J. Pridham, Robert G. Gourdie
Summary: Channels formed by Connexin (Cx43) are associated with various heart diseases, with some Cx43 mimetic peptides showing therapeutic potential and advancing to clinical testing. However, challenges in drug delivery, stability, and tissue penetration have caused reluctance in the pharmaceutical industry to translate peptidic therapeutics to the clinic. Novel drug delivery technologies like nanoparticles may provide solutions to these issues.
JOURNAL OF CARDIOVASCULAR DEVELOPMENT AND DISEASE
(2021)