期刊
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
卷 11, 期 2, 页码 389-406出版社
ELSEVIER INC
DOI: 10.1016/j.jcmgh.2020.09.004
关键词
Liver Pyruvate Kinase; Sex Differences; NAFLD; Liver Fibrosis; Mitochondrial Dysfunction
资金
- AHA [18POST33990256]
- UCLA
- UCLA Tumor Cell Biology Training grant [T32CA009056]
- [NIH-P01HL028481]
- [NIH-R01DK117850]
- [NIH-K99DK120875]
- [NIH-R35GM138003]
- [NIH-P30DK063491]
L-PK plays a role in the development of liver steatosis and fibrosis in a male-specific manner, and manipulating this gene can improve these conditions, indicating the importance of understanding sexual dimorphism in personalized NAFLD therapeutics.
BACKGROUND & AIMS: The etiology of nonalcoholic fatty liver disease (NAFLD) is poorly understood, with males and certain populations exhibiting markedly increased susceptibility. Using a systems genetics approach involving multi-omic analysis of similar to 100 diverse inbred strains of mice, we recently identified several candidate genes driving NAFLD. We investigated the role of one of these, liver pyruvate kinase (L-PK or Pklr), in NAFLD by using patient samples and mouse models. METHODS: We examined L-PK expression in mice of both sexes and in a cohort of bariatric surgery patients. We used liver-specific loss- and gain-of-function strategies in independent animal models of diet-induced steatosis and fibrosis. After treatment, we measured several metabolic phenotypes including obesity, insulin resistance, dyslipidemia, liver steatosis, and fibrosis. Liver tissues were used for gene expression and immunoblotting, and liver mitochondria bioenergetics was characterized. RESULTS: In both mice and humans, L-PK expression is up-regulated in males via testosterone and is strongly associated with NAFLD severity. In a steatosis model, L-PK silencing in male mice improved glucose tolerance, insulin sensitivity, and lactate/pyruvate tolerance compared with controls. Further-more, these animals had reduced plasma cholesterol levels and intrahepatic triglyceride accumulation. Conversely, L-PK over-expression in male mice resulted in augmented disease phe-notypes. In contrast, female mice over-expressing L-PK were unaffected. Mechanistically, L-PK altered mitochondrial pyruvate flux and its incorporation into citrate, and this, in turn, increased liver triglycerides via up-regulated de novo lipogenesis and increased PNPLA3 levels accompanied by mitochondrial dysfunction. Also, L-PK increased plasma cholesterol levels via increased PCSK9 levels. On the other hand, L-PK silencing reduced de novo lipogenesis and PNPLA3 and PCSK9 levels and improved mitochondrial function. Finally, in fibrosis model, we demonstrate that L-PK silencing in male mice reduced both liver steatosis and fibrosis, accompanied by reduced de novo lipogenesis and improved mitochondrial function. CONCLUSIONS: L-PK acts in a male-specific manner in the development of liver steatosis and fibrosis. Because NAFLD/nonalcoholic steatohepatitis exhibit sexual dimorphism, our results have important implications for the development of personalized therapeutics.
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