Article
Cell & Tissue Engineering
Sanja Novak, Josip Madunic, Laura Shum, Milan Vucetic, Xi Wang, Hitoshi Tanigawa, Mallika Ghosh, Archana Sanjay, Ivo Kalajzic
Summary: Bone regeneration relies on a group of bone/cartilage stem/progenitor cells and their differentiation is regulated by signaling mechanisms. PDGF signaling through PDGFR beta was found to inhibit BMP2-induced osteogenesis and attenuate expression of BMP2 target genes. The outcomes of treatment with two anabolic agents, PDGF and BMP2, were evaluated in different bone healing models. In vivo study showed that PDGF inhibits BMP2-induced osteogenesis, but inhibiting PDGF signaling early in the healing process does not improve BMP2-induced bone healing.
NPJ REGENERATIVE MEDICINE
(2023)
Article
Cell & Tissue Engineering
B. Q. Le, J. H. Too, T. C. Tan, R. A. A. Smith, V Nurcombe, S. M. Cool, N. Yu
Summary: The study demonstrates that a collagen/HS3 combination device enhances bone tissue regeneration, improves functional ligament restoration, enhances alveolar bone height, and reduces epithelial growth in the apical direction. The combination of collagen/HS3, collagen/BMP2 or collagen/HS3 + BMP2 results in a three to four-fold increase in bone regeneration compared to collagen alone.
EUROPEAN CELLS & MATERIALS
(2021)
Article
Orthopedics
Joseph A. Panos, Michael J. Coenen, Christopher V. Nagelli, Erin B. McGlinch, Aysegul Atasoy-Zeybek, Consuelo Lopez De Padilla, Rodolfo E. De la Vega, Christopher H. Evans
Summary: This study compared the bone regeneration in a rat femoral defect model under native, impaired, and growth factor-stimulated conditions. Native bone regeneration resulted in a fully healed defect, while impaired bone regeneration led to fibrofatty consolidation resembling an atrophic non-union. Treatment with rhBMP2 promoted rapid bone formation but resulted in inferior mechanical properties compared to natural healing. Technologies favoring endochondral regenerative ossification would improve clinical outcomes.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Article
Cell & Tissue Engineering
YoungBum Park, Sien Lin, Yan Bai, Seyedsina Moeinzadeh, Sungwoo Kim, Jianping Huang, Uilyong Lee, Ngan Fong Huang, Yunzhi Peter Yang
Summary: This study demonstrates the efficacy of a dual delivery system using an injectable hydrogel and microparticles (MPs) for bone morphogenetic protein 2 (BMP2) and insulin-like growth factor 1 (IGF1) in promoting early bone regeneration in critical-sized cranial bone defect, with the advantage of reducing the dose of BMP2.
TISSUE ENGINEERING PART A
(2022)
Article
Biochemistry & Molecular Biology
Claudia Siverino, Shorouk Fahmy-Garcia, Didem Mumcuoglu, Heike Oberwinkler, Markus Muehlemann, Thomas Mueller, Eric Farrell, Gerjo J. V. M. van Osch, Joachim Nickel
Summary: This study proposes an alternative strategy for the treatment of large bone defects by covalently immobilizing an engineered BMP2 variant to biocompatible scaffolds. The results demonstrate the translational potential of this innovative technique in inducing bone tissue formation, improving safety for patients, and reducing costs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Physiology
Hongzhou Shen, Lvyuan Li, Chenglong Zhang, Yang Chen, Hongbo Yu, Jiawen Si, Guofang Shen
Summary: A novel strategy of composite grafting with BMP2-loaded calcium phosphate cements (BMP2-CPC) and autogenous bone harvested by minimally invasive technique was developed and evaluated for alveolar bone grafting. The composite graft demonstrated satisfactory bone-repairing efficacy and improved resorption performance, making it an eligible alternative for application in alveolar bone grafting.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Cell & Tissue Engineering
Jae Wan Suh, Kyoung-Mi Lee, Eun Ae Ko, Dong Suk Yoon, Kwang Hwan Park, Hyun Sil Kim, Jong In Yook, Nam Hee Kim, Jin Woo Lee
Summary: Decreased angiogenesis contributes to delayed wound healing in diabetic patients. Recombinant human bone morphogenetic protein-2 (rhBMP2) has been shown to promote angiogenesis. However, the short half-lives of soluble growth factors, including rhBMP2, limit their use in wound healing. In this study, a novel delivery model using a protein transduction domain (PTD) formulated in a lipid nanoparticle (LNP) was proposed. The results showed that the gelatin hydrogel dressing loaded with LNP-formulated PTD-BMP2 could enhance the angiogenic function of BMP2 and improve diabetic wound healing.
JOURNAL OF TISSUE ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
WeiHang Ding, RuiQing Ji, Sai Yao, PengFei Ruan, Fei Ye, XiuLong Lou, LiPing Ni, YuChi Ji, Jie Chen, WeiFeng Ji
Summary: Hu'po Anshen decoction (HPASD) can promote the healing of traumatic brain injury (TBI) combined with a fracture by regulating the expression of bone morphogenetic protein 2 (BMP2). Our findings demonstrate that HPASD induces COX2 transcription through the BMP2-Smad1/5/9-RUNX2 axis, and then affects fracture healing through the COX2-mediated EP4-ERK1/2-RSK2-ATF4 axis.
Article
Cell Biology
Tie Liu, Wen Fang, Gang Wu, Yining Li, Janak L. Pathak, Yuelian Liu
Summary: This study investigated the bone defect healing potential of biomimetically fabricated low dose BMP2-doped CaP (BMP2.BioCaP) grafts in sheep humerus/femoral defects. The results showed that BMP2.BioCaP grafts had strong bone regenerative potential, comparable to autologous bone graft and more effective than DBB grafts, while exhibiting lower immunogenicity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Genetics & Heredity
Bach Quang Le, Tuan Chun Tan, Seong-Baek Lee, Ju Woong Jang, Young Sik Kim, Jung Soo Lee, Jae Won Choi, Padmapriya Sathiyanathan, Victor Nurcombe, Simon M. Cool
Summary: A novel biomaterial scaffold has been developed in this study, which can effectively release bone morphogenetic protein 2 (BMP2) to promote bone regeneration. The scaffold, composed of bovine collagen type I, bone granules, and heparan sulfate with increased affinity for BMP2, retained 58% of the initial BMP2 amount over 27 days and demonstrated bioactivity.
Article
Chemistry, Medicinal
Anirban Sardar, Alisha Ansari, Sampa Gupta, Shradha Sinha, Shubham Pandey, Divya Rai, Mukesh Kumar, Rabi Sankar Bhatta, Ritu Trivedi, Koneni Sashidhara
Summary: A series of compounds were designed and synthesized using molecular hybridization approach. Among them, compound 47 was identified as the most potent osteoanabolic agent, promoting osteogenesis via the non-canonical p38 pathway mediated by RUNX2 and BMP2. It has the potential to be used as a lead for future bone metabolic disorder treatment.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Endocrinology & Metabolism
Ariane Zamarioli, Gremah Adam, Kevin A. A. Maupin, Paul J. J. Childress, Alexander Brinker, Joao P. B. Ximenez, Nabarun Chakraborty, Aarti Gautam, Rasha Hammamieh, Melissa A. A. Kacena
Summary: The study found that administering BMP2 at the fracture site of mice had systemic effects on distant bones and the mechanisms of action may differ between Earth and spaceflight.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Erica Resende Oliveira, Lei Nie, Daria Podstawczyk, Ahmad Allahbakhsh, Jithendra Ratnayake, Dandara Lima Brasil, Amin Shavandi
Summary: Tissue engineering and regenerative medicine have addressed some shortcomings in the treatment of bone diseases, particularly in simulating the extracellular matrix and guiding bone repair. Combining scaffolding and growth factors shows great potential in promoting the effectiveness of bone regeneration methods.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Gastroenterology & Hepatology
Martijn A. J. Koppens, Hayley Davis, Gabriel N. Valbuena, Eoghan J. Mulholland, Nadia Nasreddin, Mathilde Colombe, Agne Antanaviciute, Sujata Biswas, Matthias Friedrich, Lennard Lee, Lai Mun Wang, Viktor H. Koelzer, James E. East, Alison Simmons, Douglas J. Winton, Simon J. Leedham
Summary: The study reveals that BMP signaling inhibits dedifferentiation of intestinal epithelial cells, and attenuation of the BMP pathway through stromal upregulation of Grem1 is essential for adaptive reprogramming in intestinal regeneration. However, the functional impact of this intervention is submaximal, suggesting potential therapeutic pathway manipulation in inflammatory bowel disease.
Article
Biochemistry & Molecular Biology
Hyun Seok, Hee-Youl Kim, Dong-Cheol Kang, Jung-Ho Park, Jong Hoon Park
Summary: The study compared the bone regeneration ability of bone scaffolds using particle and block bones with rhBMP-2, showing that rhBMP-2 with block bones resulted in significantly higher bone volume but also led to the formation of adipose tissue and void spaces.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Engineering, Biomedical
Pingping Han, Guillermo A. Gomez, Georg N. Duda, Saso Ivanovski, Patrina S. P. Poh
Summary: The dynamics of cell mechanics and epigenetic signatures play a crucial role in cell behavior and fate, affecting the outcomes of tissue regeneration. While 2D geometric substrates have been widely used to study cell mechanics in response to the extracellular microenvironment, there is limited knowledge about cell mechanobiology and epigenetics in 3D biomaterial matrices. This review explores the potential of additive manufacturing for incorporating multi length-scale geometry features on scaffolds, and discusses how scaffold geometry influences cell and nuclear mechanosensing and epigenetic modifications, ultimately impacting tissue regeneration outcomes.
ACTA BIOMATERIALIA
(2023)
Article
Orthopedics
Philippe Moewis, Rene Kaiser, Adam Trepczynski, Christoph von Tycowicz, Leonie Krahl, Ansgar Ilg, Johannes Holz, Georg N. Duda
Summary: This study aimed to analyze knee kinematics in patients with patient-specific episealer implants and found that these implants led to knee kinematics similar to healthy knees, with a medial pivot and high degree of femoral rollback during flexion. However, compared to healthy knees, the episealer implants showed higher femoral rollback and axial external rotation, suggesting possible post-operative muscle weakness and insufficient stabilization at high flexion.
KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY
(2023)
Article
Engineering, Biomedical
Alexander Kopp, Heilwig Fischer, Ana Prates Soares, Katharina Schmidt-Bleek, Christoph Leber, Henri Kreiker, Georg Duda, Nadja Kroeger, Kerstin van Gaalen, Henning Hanken, Ole Jung, Ralf Smeets, Max Heiland, Carsten Rendenbach
Summary: Bioabsorbable magnesium implants with plasma-electrolytic oxidation (PEO) surface modification show reduced degradation rate and improved tissue interaction compared to non-surface modified implants. The study evaluated the long-term degradation behavior and osseointegration of Mg-Ca-Zn (ZX00MEO) implants in vitro and in vivo. The results demonstrate the beneficial effect of PEO surface modification on bioabsorption behavior and bone regeneration.
ACTA BIOMATERIALIA
(2023)
Article
Biochemistry & Molecular Biology
Celine J. Agnes, Monzur Murshed, Adrien Takada, Bettina M. Willie, Maryam Tabrizian
Summary: Effective treatments for critical size bone defects remain challenging. 6-Bromoindirubin-3 '-Oxime (BIO), a glycogen synthase kinase 3fi inhibitor, is incorporated into a new formulation of the guanosine diphosphate cross-linked chitosan scaffold to promote osteogenic differentiation. The results of a proof-of-concept study suggest a dose-dependent response of BIO on early stages of osteogenic differentiation within the scaffold.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Medicine, General & Internal
Claudius Steffen, Katharina Duda, Dag Wulsten, Jan O. O. Voss, Steffen Koerdt, Susanne Nahles, Max Heiland, Sara Checa, Carsten Rendenbach
Summary: This study evaluates a new bite force measuring device and its feasibility in patients after mandibular resection. The device shows high accuracy and reproducibility, providing new opportunities for quantifying perioperative oral function after reconstructive surgery of the mandible.
Article
Cell Biology
Tazio Maleitzke, Tamara Dietrich, Alexander Hildebrandt, Jerome Weber, Jessika Appelt, Denise Jahn, Ellen Otto, Dario Zocholl, Shan Jiang, Anke Baranowsky, Georg N. Duda, Serafeim Tsitsilonis, Johannes Keller
Summary: The study found that procalcitonin (PCT) is applied as a sensitive biomarker to exclude bacterial infections in patients with rheumatoid arthritis (RA) flare-ups. Little is known about the pathophysiological role of PCT in RA. The study revealed that the lack of PCT protected Calca-deficient mice from joint inflammation.
INFLAMMATION RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Deeksha Malhan, Katharina Schmidt-Bleek, Georg N. N. Duda, Thaqif El Khassawna
Summary: The success of fracture healing depends on coordinated cellular and molecular events. This study analyzed the healing progression of a femoral fracture in mice and identified differential gene regulation in immune response, angiogenesis, ossification, extracellular matrix regulation, mitochondrial and ribosomal genes during healing. The study also revealed the crucial role of Serpin Family F Member 1 in angiogenesis and highlighted the significance of matrix metalloproteinase 13 and bone sialoprotein in bone mineralization. Histological analysis further supported the roles of matrix extracellular phosphoglycoprotein and extracellular signal-regulated kinase in bone homeostasis and healing. This study provides novel candidates for targeted interventions in healing and impaired healing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biophysics
Paul Kneifel, Philippe Moewis, Philipp Damm, Pascal Schuetz, Jorn Dymke, William R. Taylor, Georg N. Duda, Adam Trepczynski
Summary: Patellar complications can significantly affect the success of total knee arthroplasty. This study provides valuable information on the elastic properties and forces of the patellar tendon during specific activities in patients with telemetric total knee arthroplasties. The results contribute to a better understanding of the mechanical behavior of the patellar tendon and can be used for simulations of the whole resurfaced knee.
JOURNAL OF BIOMECHANICS
(2023)
Article
Orthopedics
Yann Tinguely, Vivian Shi, Franka Klatte-Schulz, Georg N. Duda, Benjamin R. Freedman, David J. Mooney
Summary: Tissue level properties are commonly studied using qualitative scoring methods, but quantitative analysis is needed to capture structural heterogeneity. This study combined an image recognition analysis that automatically segments cells based on shape with an added capacity to discriminate between cells in protein-rich regions. The findings suggest that tendon tissue changes during aging and injury are associated with variation in cell nuclear morphology and the appearance of region-specific subpopulations.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Review
Cell Biology
Lisa-Marie Burkhardt, Christian H. Bucher, Julia Loeffler, Charlotte Rinne, Georg N. Duda, Sven Geissler, Tim J. Schulz, Katharina Schmidt-Bleek
Summary: Patients with musculoskeletal diseases face a lower quality of life and higher medical expenses. The interaction between immune cells and mesenchymal stromal cells plays a crucial role in bone regeneration. Excessive accumulation of adipocytes has been linked to low-grade inflammation and impaired bone regeneration. This review discusses the features of bone marrow adipocytes and explores the potential therapeutic approach of targeting peroxisome proliferator-activated receptor gamma (PPARG) to enhance bone regeneration.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Tobias Winkler, Louisa Bell, Alwina Bender, Adam Trepczynski, Georg N. Duda, Alexander J. D. Baur, Philipp Damm
Summary: This study assessed the status of periarticular muscles in long-term follow-up after TKA and found that fatty infiltration reduction may lead to increased tibio-femoral joint contact forces. Functional muscle recovery after surgery is important for reducing joint loads and improving long-term outcomes of TKA.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Medicine, General & Internal
Luis Becker, Friederike Schoemig, Lea Marie-Sophie Cordes, Georg N. Duda, Matthias Pumberger, Hendrik Schmidt
Summary: A study was conducted with 523 participants, including 120 with low back pain and 356 without symptoms. It was found that participants with low back pain had reduced pelvic and lumbar range of motion and increased finger-floor distance compared to the asymptomatic control group. Lumbar flexibility assessment should take into account the correlation of pelvic and lumbar motion.
Article
Biology
Julia Loeffler, Anne Noom, Agnes Ellinghaus, Anke Dienelt, Stefan Kempa, Georg N. Duda
Summary: Bone regeneration after fracture is a complex process with high and dynamic energy demands. Metabolites, particularly succinate, are shown to play central roles as signaling molecules during the onset of healing and in steering bone tissue regeneration. Succinate is found to promote vascularization and ossification, highlighting that metabolites have the potential to be used therapeutically to promote fracture healing.
COMMUNICATIONS BIOLOGY
(2023)
Review
Endocrinology & Metabolism
L. Gabel, K. Kent, S. Hosseinitabatabaei, A. J. Burghardt, M. B. Leonard, F. Rauch, B. M. Willie
Summary: The purpose of this review is to summarize current approaches and provide recommendations for imaging bone in pediatric populations using high-resolution peripheral quantitative computed tomography (HR-pQCT). Three established protocols for HR-pQCT imaging in children and adolescents are presented, along with their advantages and disadvantages. The review also outlines special cases and provides tips for acquiring and processing scans to minimize motion artifacts and account for growing bone.
CURRENT OSTEOPOROSIS REPORTS
(2023)
Article
Orthopedics
Soren Overgaard, Thomas M. Grupp, Rob G. H. H. Nelissen, Luca Cristofolini, Anne Lubbeke, Marcus Jaeger, Matthias Fink, Sabine Rusch, Hassan Achakri, Francesco Benazzo, Dario Bergadano, Georg N. Duda, Christian Kaddick, Volkmar Jansson, Klaus -Peter Guenther
Summary: The new MDR 2017/745 implemented by the European Parliament requires more robust clinical and pre-clinical data. The EFORT Implant and Patient Safety Initiative WG1 'Introduction of Innovation' developed comprehensive recommendations for the introduction of innovations in joint arthroplasty under the boundaries of MDR 2017/745. These recommendations were developed with the collaboration of orthopaedic surgeons, research institutes, orthopaedic device manufacturers, patient representatives, and regulatory authorities.
EFORT OPEN REVIEWS
(2023)
