期刊
VACCINES
卷 8, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/vaccines8030513
关键词
swine; foot-and-mouth disease virus; dendrimer peptide; swine leukocyte antigen; SLA polymorphism; adaptive immunity; vaccine; protection
资金
- Spanish Ministry of Science, Innovation and Universities [AGL2017-89097-C2, AGL2016-76445-R, PGC2018-096849-B-I00]
- Comunidad de Madrid
- Generalitat de Catalunya [2009SGR492]
- Fundacion Ramon Areces
- Maria de Maeztu Program of the Spanish Ministry of Science, Innovation and Universities
- Spanish Ministry of Science, Innovation and University (FPI programme)
- ECFEDER funds [P2018/BAA-4370]
Dendrimer peptides are promising vaccine candidates against the foot-and-mouth disease virus (FMDV). Several B-cell epitope (B2T) dendrimers, harboring a major FMDV antigenic B-cell site in VP1 protein, are covalently linked to heterotypic T-cell epitopes from 3A and/or 3D proteins, and elicited consistent levels of neutralizing antibodies and IFN-gamma-producing cells in pigs. To address the contribution of the highly polymorphic nature of the porcine MHC (SLA, swine leukocyte antigen) on the immunogenicity of B2T dendrimers, low-resolution (Lr) haplotyping was performed. We looked for possible correlations between particular Lr haplotypes with neutralizing antibody and T-cell responses induced by B2T peptides. In this study, 63 pigs immunized with B2T dendrimers and 10 non-immunized (control) animals are analyzed. The results reveal a robust significant correlation between SLA class-II Lr haplotypes and the T-cell response. Similar correlations of T-cell response with SLA class-I Lr haplotypes, and between B-cell antibody response and SLA class-I and SLA class-II Lr haplotypes, were only found when the sample was reduced to animals with Lr haplotypes represented more than once. These results support the contribution of SLA class-II restricted T-cells to the magnitude of the T-cell response and to the antibody response evoked by the B2T dendrimers, being of potential value for peptide vaccine design against FMDV.
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