Article
Engineering, Environmental
Shu-Ting Zhang, Tao Li, Shi-Kai Deng, Jim C. Spain, Ning-Yi Zhou
Summary: This study reveals a novel bacterial metabolic pathway for the degradation of 4-nitroanisole, initiated by a three-component cytochrome P450 system (pnaABC). This system exhibits broad substrate specificity and can catalyze the degradation of nitroanisole derivatives.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Yanhui Lu, Qi Bai, Qiang Li, Xusong Zheng, Junce Tian, Jiawen Guo, Hongxing Xu, Zhongxian Lu
Summary: The study identified and characterized two cytochrome P450 monooxygenase genes, CsCYP6SN3 and CsCYP306A1, associated with the lethal effect of vetiver against C. suppressalis larvae. Inhibition of these genes significantly reduced pupation rate and pupa weight in the pests.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biology
Dorina Strieth, Andreas Weber, Johannes Robert, Judith Stiefelmaier, Jonas Kollmen, Marianne Volkmar, Michael Lakatos, Volkmar Jordan, Kai Muffler, Roland Ulber
Summary: Aerosol-based photobioreactor was developed for cultivating phototrophic biofilms, resulting in higher productivity. Experiments and simulation showed that cultivation surface had no influence on biomass production but affected biofilm thickness.
Article
Biochemistry & Molecular Biology
Narayanan Srividya, Iris Lange, Jana K. Richter, Matthias Wuest, B. Markus Lange
Summary: In this study, the enantioselectivity of multiple enzymes involved in monoterpenoid biosynthesis in peppermint and Japanese catnip was investigated. The experimental results showed that these enzymes exhibited different enantioselectivity towards intermediates of the pathway, and molecular modeling studies supported the experimental data. This study lays the groundwork for further structure-function studies on the origin of enantioselectivity in closely related species of the Lamiaceae.
Article
Chemistry, Multidisciplinary
Danilo Correddu, Sabrina Helmy Aly, Giovanna Di Nardo, Gianluca Catucci, Cristina Prandi, Marco Blangetti, Chiara Bellomo, Elisabetta Bonometti, Guido Viscardi, Gianfranco Gilardi
Summary: This study found that the cytochrome P450 BM3 mutants have enhanced and specific epoxidation activity for geraniol and linalool, forming valuable oxidized derivatives, highlighting the significance of these enzymes for industrial applications.
Article
Biochemistry & Molecular Biology
Jiawei Liu, Hongmin Hu, Huimin Shen, Qingyin Tian, Wenjie Ding, Xiulian Yang, Lianggui Wang, Yuanzheng Yue
Summary: This study identified a total of 276 P450 genes belonging to 40 families in Osmanthus fragrans, suggesting their potential roles in fragrance synthesis. The research provides valuable insights into the functions of P450 genes and serves as a guide for further studies on promoting fragrance biosynthesis in ornamental plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Hongsheng Gao, Huiqing Huang, Kaifeng Lu, Cuiting Wang, Xiaohua Liu, Zhizhong Song, Houjun Zhou, Lei Yang, Bei Li, Chunyan Yu, Hongxia Zhang
Summary: The expression of OsCYP714D1 gene improved salt tolerance in transgenic poplar plants by regulating gibberellin and ion homeostasis, resulting in increased plant height, K+ content, and decreased growth inhibition and Na+ content under high salt stress conditions. Transcriptomic analyses revealed up-regulation of GA biosynthesis, signaling, and stress responsive genes in transgenic plants, while gene ontology analyses indicated enrichment of genes involved in plant hormone and ion metabolic activities in the transgenic plants. OsCYP714D1 can be used as a candidate gene for engineering new tree varieties with improved biomass production and salt stress resistance.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Pakjira Nanudorn, Sirinthra Thiengmag, Friederike Biermann, Pelin Erkoc, Sabrina D. Dirnberger, Thao N. Phan, Robert Fuerst, Reiko Ueoka, Eric J. N. Helfrich
Summary: This study discovered a new family of ribosomally synthesized and posttranslationally modified peptides, named atropopeptides. The characterization of two atropopeptide biosynthetic pathways revealed a two-step maturation process. Atropopeptides promote pro-angiogenic cell functions.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Gabriela C. Schroeder, Martha S. Smit, Diederik J. Opperman
Summary: CYPs/P450s are versatile hemethiolate proteins with high stereo- and regio-selectivity, making them powerful biocatalysts for synthesizing value-added compounds. This review highlights recent advances in P450-mediated syntheses, including enzyme engineering strategies to enhance selectivity and facilitate bond formation, as well as approaches to overcome limitations of P450 catalysis for improved efficiency and biotechnological viability.
CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY
(2023)
Article
Chemistry, Physical
Pradeep Subedi, Jong Kook Park, Tae-Jin Oh
Summary: This study reported the catalytic capabilities of a bacterial cytochrome P450 enzyme, CYP105A5, towards different flavonoids and enhanced the product formation rate through rational design. The double mutant L100A/I302A showed significant improvement in flavonoid hydroxylation. These findings may facilitate the application of CYP105A5 in whole-cell biocatalysts for the production of valuable polyphenols.
Article
Agriculture, Multidisciplinary
Carlos A. G. Rigon, Luan Cutti, Guilherme M. Turra, Enrico Z. Ferreira, Christian Menegaz, Walker Schaidhauer, Franck E. Dayan, Todd A. Gaines, Aldo Merotto
Summary: Herbicide mixtures are commonly used to control a wide range of weeds, including those resistant to specific herbicides. However, the impact of mixtures on the evolution of herbicide resistance through enhanced metabolism is not well understood. In this study, the effects of a fenoxaprop-p-ethyl and imazethapyr mixture on herbicide resistance evolution in Echinochloa crus-galli were evaluated. Results showed that recurrent selection with the mixture led to decreased control and increased resistance to several herbicides. Additionally, it was found that the main contributor to decreased control was fenoxaprop. This study highlights the need for using fully recommended herbicide rates in mixtures to reduce the risk of resistance evolution.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agronomy
Tianfei Peng, Xuemei Liu, Fayi Tian, Hongfei Xu, Fengting Yang, Xuewei Chen, Xiwu Gao, Yuntong Lv, Jianyi Li, Yiou Pan, Qingli Shang
Summary: The study revealed that the upregulation of certain cytochrome P450 genes is associated with spirotetramat resistance in A. gossypii, and some lncRNAs may regulate the expression of these P450 genes to affect resistance. These findings provide a molecular basis for further investigating cotton aphid P450 genes and lncRNA functions in spirotetramat resistance development.
PEST MANAGEMENT SCIENCE
(2022)
Article
Environmental Sciences
Mlamuli Ndabambi, Ki Yong Kim, Jee-Hyun Jung, Un-Hyuk Yim, Jeong-Eun Oh
Summary: The study investigated the biotransformation and tissue distribution of methylated phenanthrenes in black rockfish, and identified these compounds as potential biomarkers of rockfish exposure to petrogenic PAHs that can be effectively eliminated from the fish's body.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Entomology
Jing Yang, Buli Fu, Peipan Gong, Chengjia Zhang, Xuegao Wei, Cheng Yin, Mingjiao Huang, Chao He, Tianhua Du, Jinjin Liang, Shaonan Liu, Yao Ji, Hu Xue, Chao Wang, Jinyu Hu, He Du, Rong Zhang, Xin Yang, Youjun Zhang
Summary: In this study, we investigated the role of CYP6CX2 and CYP6CX3 in mediating whitefly thiamethoxam resistance. Our results showed that the mRNA levels of these two genes were up-regulated after exposure to thiamethoxam. Transcriptional levels were overexpressed in resistant strains and positively correlated with resistance levels. Silencing these genes increased whitefly susceptibility to thiamethoxam. These findings provide insights into the role of cytochrome P450 monooxygenases in neonicotinoid resistance and suggest their potential as target genes for pest management.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kai Tian, Jing Feng, Jiang Zhu, Jiagao Cheng, Mei Li, Xinghui Qiu
Summary: The study found that three paralogous CYP9As were capable of metabolizing multiple pyrethroids, with a greater efficiency in transforming pyrethrin-resembling pyrethroids. These results support the hypothesis that herbivorous insects can cope with synthetic insecticides through their physiological systems.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Biochemical Research Methods
Diego Salamanca, Katja Buehler, Karl-Heinrich Engesser, Andreas Schmid, Rohan Karande
Summary: This study investigates the use of an Acidovorax mutant as a whole-cell biocatalyst for converting cycloalkanes to omega-hydroxycarboxylic acids, showcasing high metabolic efficiency with an efficient enzyme cascade. The deletion of a specific enzyme in the metabolic pathway led to the production of short-chain omega-hydroxycarboxylic acids, demonstrating a high molar conversion rate above 98% within 6 hours.
Article
Biotechnology & Applied Microbiology
Lisa Bretschneider, Martin Wegner, Katja Buehler, Bruno Buehler, Rohan Karande
Summary: A bioprocess for synthesizing 6-aminohexanoic acid (6AHA) was developed using a mixed-species approach with promising results. The combination of Pseudomonas taiwanensis and Escherichia coli strains allowed for high conversion and yield of 6AHA under environmentally benign conditions.
MICROBIAL BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ingeborg Heuschkel, Selina Hanisch, Daniel C. Volke, Erik Loefgren, Anna Hoschek, Pablo Nikel, Rohan Karande, Katja Buehler
Summary: The study investigated the biocatalytic performance of a BVMO catalyzing the reaction of cyclohexanone to epsilon-caprolactone in Pseudomonas biofilms, with Ps_BVMO_DGC biofilms showing faster growth compared to Ps_BVMO biofilms in DFRs. However, challenges in RBRs such as low biofilm surface coverage, side-product formation, and oxygen limitation led to a drop in production rates over time.
ENGINEERING IN LIFE SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Lisa Bretschneider, Ingeborg Heuschkel, Afaq Ahmed, Katja Buehler, Rohan Karande, Bruno Buehler
Summary: The study characterized and compared the Acidovorax-derived CHMO gene expressed in P. taiwanensis VLB120. Biofilms showed less favorable K-S and k(cat) values, but a favorable K-I for cyclohexanone; suspended cells format exhibited higher potential from a kinetics perspective.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Francesco Scarabotti, Katja Buehler, Matthias Schmidt, Falk Harnisch
Summary: This study analyzed the influence of thickness and roughness of AuPd electrodes on the physiological parameters of early-stage biofilms of G. sulfurreducens using confocal laser scanning microscopy. The results showed that electrode preparation did not affect the maximum current density, lag time, and single cell yield coefficients of the biofilms, confirming the robustness of the experimental approach.
BIOELECTROCHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Lisa Bretschneider, Ingeborg Heuschkel, Katja Buehler, Rohan Karande, Bruno Buehler
Summary: This study successfully constructed a strain of Pseudomonas taiwanensis that efficiently converts cyclohexane to adipic acid, laying the foundation for environmentally friendly production of adipic acid and related polymers.
METABOLIC ENGINEERING
(2022)
Article
Energy & Fuels
Francesco Scarabotti, Anne Kuchenbuch, Rene Kallies, Katja Buehler, Falk Harnisch
Summary: This study demonstrates that physiological cellular parameters of electroactive microorganisms can be non-invasively and in real-time obtained during early-stage biofilm formation at electrodes using a microfluidic electrochemical flow-cell. Parameters for mixed culture biofilm anodes formed at +150 mV vs. Ag/AgCl sat. KCl include lagt, CPR, t(d), RGR, and Y-Ne.
FRONTIERS IN ENERGY RESEARCH
(2022)
Article
Agricultural Engineering
Jorg Toepel, Rohan Karande, Bruno Buehler, Katja Buehler, Andreas Schmid
Summary: Hydrogen (H2) is a promising fuel for climate neutral energy carriers, and photosynthesis-driven H2 production, which relies on sunlight and water, has shown potential. Research has been conducted using cyanobacteria as biocatalysts in capillary biofilm photobioreactors (CBRs). The study found that biofilm formation and stability are influenced by light and CO2 availability, and H2 production rates vary between different cyanobacteria species. By adjusting light and O2 levels, biofilm formation and H2 production could be enhanced, with high stability lasting for weeks. Final biocatalyst titers reached up to 100 g l-1, and H2 production rates were up to 300 mu mol H2 l-1h-1 and 3 mu mol H2 gcdw-1h-1 in biofilms.
BIORESOURCE TECHNOLOGY
(2023)
Article
Microbiology
Mahir Bozan, Denny Popp, Rene Kallies, Ulisses Nunes da Rocha, Stephan Klaehn, Katja Buehler
Summary: Cyanobacteria have great potential in biotechnologies, and this study focuses on the development of molecular tools for genetic engineering, as well as the design of chassis strains and novel reactor concepts. The genome sequence of Tolypothrix sp. PCC 7712 is provided, and it is found to have high similarities with Tolypothrix sp. PCC 7601, but also some specific variations. These findings are important for targeted strain engineering.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Biochemical Research Methods
Joerg Toepel, Rohan Karande, Stephan Klaehn, Bruno Buehler
Summary: Cyanobacteria have great potential to produce chemicals from sustainable resources through photosynthesis. Recent research on internal electron and carbon fluxes and their regulation provides new opportunities for efficient product formation. Novel photobioreactor concepts are being developed to ensure sufficient light supply. This review summarizes the latest developments in cyanobacterial engineering and highlights the importance of a holistic approach to establish photosynthesis-based production processes for a future green bioeconomy.
CURRENT OPINION IN BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Amelie Kenkel, Rohan Karande, Katja Buehler
Summary: The suitability of capillary biofilm reactors (CBRs) for growing an artificial consortium of Synechocystis sp. PCC 6803 and Pseudomonas sp. VBL120 was evaluated. It was found that silicone material has excellent oxygen permeability, and high flow rates and appropriate medium composition can enhance biomass development and biofilm stability. Increasing trace elements allows for successful scale-up of CBR.
ENGINEERING IN LIFE SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Micjel Chavez Morejon, Alexander Franz, Rohan Karande, Falk Harnisch
Summary: We demonstrate a method for synthesizing adipic acid (AA), a key component of polyamides like Nylon-6,6, from lignin-derived feedstock using a combination of electrochemical hydrogenation and biotransformation. The electrochemical hydrogenation process achieves a 68% yield of cyclohexanol from phenol at a 69% coulombic efficiency, and the electrochemical hydrogenation of an aromatic mixture obtained from lignin depolymerization yields up to 83%. By further biotransformation with recombinant Pseudomonas taiwanensis VLB120, AA is produced at a rate of 0.02 g L-1 h(-1) with a yield of up to 61% in 5 hours.
Article
Microbiology
Mahir Bozan, Andreas Schmid, Katja Buehler
Summary: Cyanobacteria are promising microorganisms for sustainable photo-biotechnological production processes. In this study, different cyanobacteria were evaluated for their application in a novel capillary biofilm reactor, and Tolypothrix sp. PCC 7712 emerged as the best performer.