Article
Food Science & Technology
Pedro Valencia, Karen Espinoza, Carolina Astudillo-Castro, Fernando Salazar
Summary: Systematic modeling of enzymatic hydrolysis of milk proteins was conducted in this study. The modeling tool, which combined logarithmic and polynomial equations, accurately predicted the changes in kinetic constants and hydrolysis curves of milk proteins. This model can be used to test the correlation between the degree of hydrolysis and the functional properties of milk hydrolysates.
Review
Agricultural Engineering
Hongliang Guo, Ying Zhao, Jo-Shu Chang, Duu-Jong Lee
Summary: Enzymatic hydrolysis is crucial for efficient biorefinery of lignocellulosic biomass. Understanding the properties of key enzymes and their interactions with biomass is essential for improving the process efficiency. This article provides an update on the research of key enzymes in lignocellulose biorefinery, including their properties, interactions with biomass, adsorption, and hydrolysis. The advanced analytical techniques for investigating these interactions and future research prospects are discussed.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
M. E. Russo, A. Procentese, F. Montagnaro, A. Marzocchella
Summary: The use of waste lignocellulose feedstock for sugar-based biorefineries is of interest to scientists and industries. This study characterized the enzymatic hydrolysis kinetics of coffee silverskin and provided an optimized process that can be applied to various biomasses.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Mar Serrano-Arnaldos, M. Dolores Murcia, Salvadora Ortega-Requena, M. Claudia Montiel, Fuensanta Maximo, Elisa Gomez, Josefa Bastida
Summary: The pseudo-first-order kinetic model accurately describes the esterification process of cetyl esters and predicts the reaction rate of spermaceti. The results suggest that lipases can be used as industrial catalysts with the assistance of this simple yet accurate kinetic model.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2021)
Article
Engineering, Chemical
Cristine De Pretto, Liceres Correa de Miranda, Paula Fernandes de Siqueira, Marcelo Perencin de Arruda Ribeiro, Roberto de Campos Giordano, Raquel de Lima Camargo Giordano, Caliane Bastos Borba Costa
Summary: This study aimed to model the enzymatic hydrolysis of proteins present in soybean protein concentrate meal using two steps. In the first step, the concentration of hydrolyzed peptide bonds was described as a function of time, using two kinetic models. In the second step, the way the enzyme breaks the protein down into smaller parts was investigated and modeled using an artificial neural network. The study found that while pH influenced the behavior of the reaction rate and the time profile of the degree of hydrolysis, it did not explicitly affect the peptide profile.
CHEMICAL ENGINEERING COMMUNICATIONS
(2022)
Article
Agriculture, Multidisciplinary
Siti Hanifah Adiiba, Eng-Seng Chan, Yee Ying Lee, Amelia, Mun Yuen Chang, Cher Pin Song
Summary: This study developed a methanol-free enzymatic hydrolysis process to produce palm phytonutrients from crude palm oil (CPO). The results showed that the ET 2.0 enzyme was the most effective for hydrolysis, achieving a high FFA content under optimized conditions. Furthermore, the addition of ascorbic acid helped retain carotenoid and tocol content.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Materials Science, Multidisciplinary
Kotchaporn Thangunpai, Donghao Hu, Mikio Kajiyama, Marcos A. A. Neves, Toshiharu Enomae
Summary: Plastic waste is a global issue due to landfill overflow and pollution, leading to environmental concerns. To address this, biodegradable materials like poly-e-caprolactone (PCL) are introduced. However, PCL's natural decomposition rate is low. To increase decomposition rate, PCL is modified with maleic anhydride (MA) (PCL-g-MA), increasing hydrophilicity and amorphous region. Lipase hydrolysis experiments show that PCL-g-MA degrades faster than PCL. Morphological, thermal, and structural analysis support the hydrolysis reaction, confirming the effectiveness of the modification.
MACROMOLECULAR MATERIALS AND ENGINEERING
(2023)
Article
Multidisciplinary Sciences
Maryam Khan, Zubair Ahmad, Farhad Ali, Naveed Khan, Ilyas Khan, Kottakkaran Sooppy Nisar
Summary: This research focuses on the modelling and dynamics of enzyme processes based on chemical kinetics. A two-step reversible reaction of substrate-enzyme is investigated and transformed into a fractional-order model using the Atangana-Baleanu operator and Mittag-Leffler kernel. The numerical results obtained through the Atangana-Toufik method show the dynamics of the reaction against different reaction rates.
Article
Biotechnology & Applied Microbiology
Zhenggang Gong, Guangxu Yang, Junlong Song, Peitao Zheng, Jing Liu, Wenyuan Zhu, Liulian Huang, Lihui Chen, Xiaolin Luo, Li Shuai
Summary: Peanut protein, as a lignin blocking additive, improves enzymatic hydrolysis efficiency and supports the economic viability of biorefineries.
BIORESOURCES AND BIOPROCESSING
(2021)
Article
Chemistry, Physical
M. Legree, J. -L. Bobet, F. Mauvy, J. Sabatier
Summary: This study proposes a new modeling approach to describe the hydrolysis reaction of alloys in simulated seawater solution. The model allows for discrimination of the contribution of different phases and is applicable to the entire kinetics curve.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2022)
Article
Chemistry, Multidisciplinary
Anamaria Todea, Sara Fortuna, Cynthia Ebert, Fioretta Asaro, Stefano Tomada, Marco Cespugli, Fabio Hollan, Lucia Gardossi
Summary: The lipase-catalyzed polycondensation of azelaic acid and glycerol was investigated using a Design-of-Experiment approach. The study provided rational guidelines for optimizing the reaction and successfully scaled it up for industrial use.
Article
Agricultural Engineering
Peiqian Zhang, Mei Cui, Renliang Huang, Wei Qi, Wim Thielemans, Zhimin He, Rongxin Su
Summary: This study investigated the mechanism of expansins and endoglucanase on cellulose using real-time monitoring technology. The addition of zinc ions was found to increase the adsorption rate and hydrolysis rate of both expansins and endoglucanase.
BIORESOURCE TECHNOLOGY
(2021)
Article
Chemistry, Organic
Ali Nejatie, Cinzia Colombo, Benyamin Hakak-Zargar, Andrew J. Bennet
Summary: Sialic acids are important carbohydrates found widely in nature. We investigated their reactivity in aqueous solution, specifically focusing on the differences in reactivity between N-acetylneuraminic acid and Kdn. Our findings suggest that C5-oxygen containing sialosides have larger hydrolytic rate constants than their parent C-5 acetamido glycoside.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Agricultural Engineering
Qihong Zhang, Zhaohui Lu, Chen Su, Zongmiao Feng, Hui Wang, Jingbo Yu, Weike Su
Summary: This study successfully improved the yield and production efficiency of CNCs through mechano-enzymatic hydrolysis, avoiding the use of bulk water and reducing production costs.
BIORESOURCE TECHNOLOGY
(2021)
Article
Materials Science, Multidisciplinary
Li Xu, Aiting Zhang, Ruijie Liao, Yuxia Pang, Dongjie Yang, Hongming Lou, Xueqing Qiu
Summary: By synthesizing a cationic copolymer, cellulase adsorption onto lignin surface can be effectively obstructed, leading to enhanced glucose release. The copolymers show versatility in different enzyme preparations, acid-treated lignocellulosic substrates, and solid saccharification systems, while also achieving enzyme saving. Mechanistically, the copolymer attenuates irreversible binding between lignin and cellulase and exerts a positive influence on enzyme activity.
ACS APPLIED POLYMER MATERIALS
(2023)
Article
Engineering, Chemical
Bernhard Schaefer, Felipe Andres Toro Chacon, Thorsten Brinkmann, Anja Drews, Eberhard Jochem, Joerg Sauer
Summary: The EDUAR&D methodology is used to evaluate energy efficiency technologies and derive recommendations for future energy research and energy policy.
CHEMIE INGENIEUR TECHNIK
(2021)
Article
Energy & Fuels
Nico Jurtz, Urvashi Srivastava, Alireza Attari Moghaddam, Matthias Kraume
Summary: The study focused on process intensification of catalytic fixed-bed reactors, conducting optimization of heat transport characteristics on the pellet scale using computational fluid dynamics. Evaluation of key performance indicators such as the global heat transfer coefficient and the specific pressure drop was carried out to compare thermal performance of different designs.
Review
Chemistry, Multidisciplinary
Mathias Heiker, Matthias Kraume, Anica Mertins, Tim Wawer, Sandra Rosenberger
Summary: Biogas production is a key component in renewable energy systems, with modeling approaches ranging from detailed anaerobic digestion process modeling to aggregated regional biogas potential modeling. The different levels of detail in these models, ranging from white box to black box approaches, offer opportunities for dynamic business models of biogas usage in energy systems.
APPLIED SCIENCES-BASEL
(2021)
Article
Engineering, Chemical
Thomas Eppinger, Nico Jurtz, Matthias Kraume
Summary: Using three-dimensional particle-resolved discrete element method computational fluid dynamics simulations, it is possible to improve the heat transfer efficiency of fixed bed reactors by using macroscopic wall structures to reduce the non-uniform distribution of particles near the reactor wall, resulting in better radial heat transfer. The study found that this method can increase heat transfer efficiency by up to 40%, reduce the thermal entry length by 66%, and increase overall heat transfer by up to 120%.
Article
Energy & Fuels
Azis Boing Sitanggang, Anja Drews, Matthias Kraume
Summary: Enzymatic membrane reactors (EMRs) are increasingly used in biotechnology, (bio)chemistry, agro-food, and energy sectors due to their feasibility of running continuous enzymatic reaction and product separation in a single operation. This review discusses available designs of EMRs based on membrane functions, hydrodynamic and filtration characteristics, and outlines various applications of EMRs in agro-food, pharmaceutical, environmental, and energy sectors. Fouling, a well-known problem in membrane technology, and its circumventions in size-exclusion EMR are critically discussed.
CHEMICAL ENGINEERING AND PROCESSING-PROCESS INTENSIFICATION
(2022)
Article
Engineering, Chemical
Maresa Vivien Kempin, Hendrik Schroeder, Lena Hohl, Matthias Kraume, Anja Drews
Summary: The feasibility and efficiency of Pickering emulsion membrane filtration for catalyst recovery in continuous liquid/liquid multiphase catalysis were demonstrated. Experimental investigation showed that temperature affected the permeation of solvent and filtration of Pickering emulsions, while the type of silica nanoparticles had a negligible impact. A mathematical model was successfully developed to predict the flux of solvent through the membrane and accurately describe the Pickering emulsion filtration behavior, providing valuable insights for optimal process design in catalytic multiphase systems.
JOURNAL OF MEMBRANE SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Roy Nitzsche, Hendrik Etzold, Marlen Verges, Arne Groengroeft, Matthias Kraume
Summary: This study demonstrated and evaluated two purification cascades for the separation and valorization of hemicellulose and its derived monomeric sugars from BWHs, showing their efficiency and cost.
Article
Chemistry, Physical
Christoph Plikat, Anja Drews, Marion B. Ansorge-Schumacher
Summary: This study provides insight into the effects of proteins on the fineness and stability of water-in-oil Pickering emulsions, as well as their catalytic performance. The presence of proteins resulted in decreased droplet sizes and stability against coalescence, with lipase concentration and location being crucial factors for optimizing catalytic performance.
Article
Chemistry, Multidisciplinary
Sebastian Stock, Susanne Roehl, Luca Mirau, Matthias Kraume, Regine von Klitzing
Summary: The incorporation of soft hydrophilic particles at the interface of water in non-polar oil emulsion droplets is crucial, but it is not possible to stabilize the emulsions with hydrophilic soft material alone. Previous works have shown that using hydrophobic silica nanospheres and charged microgel particles is an effective strategy to stabilize w/o emulsions while incorporating a large amount of microgel particles at the interface.
Article
Engineering, Chemical
Patrik Vukovic, Wolfgang Kessler, Matthias Kraume
Summary: The current study proposes a new method to purify crosslinked hydrogels based on hyaluronic acid (HA) by utilizing crossflow filtration (CFF) device. The results show that a dynamic diafiltration process can effectively remove residual contaminants while maintaining the thermal stability and rheological characteristics of the biopolymer. The rejection coefficient of HA remains consistent regardless of the membrane pore size, indicating the promising potential of this method as an alternative to conventional purification methods.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Isabel Thiele, Heba Yehia, Niels Krausch, Mario Birkholz, Mariano Nicolas Cruz Bournazou, Azis Boing Sitanggang, Matthias Kraume, Peter Neubauer, Anke Kurreck
Summary: Nucleoside analogues are important for treating viral infections or cancers. Continuous enzyme membrane reactors (EMRs) offer an alternative to batch enzymatic reactions, allowing for continuous production with integrated biocatalyst separation. The EMR demonstrated comparable specific productivities to batch reactions, but with significantly higher product per enzymatic unit for all nucleosides synthesized. This study validates the application of EMRs for nucleoside synthesis, reducing production costs and improving downstream processing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Susanne Roehl, Lena Hohl, Sebastian Stock, Manlin Zhan, Tobias Kopf, Regine von Klitzing, Matthias Kraume
Summary: In this study, a first approach to model drop size distributions in agitated nanoparticle-stabilized liquid/liquid systems with population balance equations is presented. A novel modified coalescence efficiency is developed for the population balance framework, considering the effect of steric hindrance of nanoparticles at the liquid/liquid interface. The modified submodel is validated with experimental data of water-in-oil dispersions stabilized by silica nanoparticles.
Article
Thermodynamics
Henning Junne, Nico Jurtz, Joschka M. Schulz, Matthias Kraume, Lutz Boehm
Summary: The authors propose a new algorithm for analyzing ray deflection within spherical refractive index fields. Numerical validation confirmed the feasibility of using this algorithm to determine spherical refractive index fields in experiments.
NUMERICAL HEAT TRANSFER PART B-FUNDAMENTALS
(2023)
Article
Engineering, Chemical
Stefan Seidel, Fruhar Mozaffari, Rudiger W. Maschke, Matthias Kraume, Regine Eibl-Schindler, Dieter Eibl
Summary: Scaling up bioprocesses is a major challenge, requiring considerations such as specific power input. Using CFD to determine the Kolmogorov length scale distribution allows for scaling up geometrically non-similar bioreactors. Automated optimization can achieve comparable cultivation results and predict cell aggregate size distribution, even at pilot scale.
Article
Chemistry, Physical
Saeid Dowlati, Aliyar Javadi, Reinhard Miller, Kerstin Eckert, Matthias Kraume
Summary: Unfolding can deactivate enzymes and significantly affect the yield in biochemical engineering. Urea-induced unfolding of lipase destructs its structure and reduces the viscoelasticity of the adsorbed layer.
COLLOIDS AND INTERFACES
(2022)