Article
Cell Biology
Yanchun Li, Xin Wang, Zhihui Huang, Yi Zhou, Jun Xia, Wanye Hu, Xu Wang, Jing Du, Xiangmin Tong, Ying Wang
Summary: CISD3 plays a crucial regulatory role in cancer progression and ferroptosis, with its overexpression associated with poorer survival rates, while its depletion accelerates ferroptotic cell death. Depletion of CISD3 leads to metabolic reprogramming towards glutaminolysis, which is essential for mitochondrial oxidative phosphorylation.
CELL DEATH & DISEASE
(2021)
Article
Oncology
Giulia Pinton, Zhuo Wang, Cecilia Balzano, Sara Missaglia, Daniela Tavian, Renzo Boldorini, Dean A. Fennell, Martin Griffin, Laura Moro
Summary: Malignant pleural mesothelioma is an aggressive cancer with heterogeneous presentation and behavior, and despite increasing knowledge about molecular markers, they are not fully utilized for treatment allocation. Recent studies have shown that mesothelioma cells lacking BAP1 are sensitive to EZH2 histone methyltransferase inhibition. Manipulating SIRT1 expression and culturing cells as multicellular spheroids can enhance the sensitivity of BAP1 wild-type mesothelioma cells to EPZ-6438, ultimately leading to cell growth arrest and gene expression alterations. Loss of CDKN2A predicts cell fate in response to EZH2 inhibition, suggesting its potential use in stratifying tumors likely to undergo apoptosis.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yulin Zhang, Yang Kong, Yuan Ma, Shilei Ni, Tobias Wikerholmen, Kaiyan Xi, Feihu Zhao, Zhimin Zhao, Junpeng Wang, Bin Huang, Anjing Chen, Zhong Yao, Mingzhi Han, Zichao Feng, Yaotian Hu, Frits Thorsen, Jian Wang, Xingang Li
Summary: The research identified COPZ1 as a therapeutic target candidate for GBM due to its role in dysregulating iron metabolism, leading to increased tumor grade and poor prognosis. Suppression of COPZ1 through siRNA knockdown inhibited proliferation and tumor growth of GBM cells both in vitro and in vivo, suggesting COPZ1/NCOA4/FTH1 axis as a potential novel therapeutic target for human GBM.
Review
Cell Biology
Xin Chen, Paul B. Comish, Daolin Tang, Rui Kang
Summary: The induction and consequences of regulated cell death (RCD) result in changes in gene and protein expression, biochemical pathways, cell morphology, and size. Among different forms of RCD, ferroptosis, driven by iron overload and lipid peroxidation, is implicated in various pathological conditions and diseases. The ferroptotic response can be fine-tuned through oxidative stress and antioxidant pathways, and a series of inducers or inhibitors have been developed targeting metabolism-related proteins.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Chenyao Wu, Zhonglong Liu, Zhixin Chen, Deliang Xu, Lisong Chen, Han Lin, Jianlin Shi
Summary: This study introduces a non-ferrous ferroptosis-like strategy based on a hybrid CoMoO4-phosphomolybdic acid nanosheet (CPMNS), which achieves efficient anticancer efficacy through specific mechanisms. The high feasibility of this approach in nanocatalytic medicine is expected to advance cancer therapeutic regimens in the future.
Article
Oncology
Yingkai Hong, Mingen Lin, Dehua Ou, Zhuangkai Huang, Peilin Shen
Summary: A prognostic 12-gene signature related to ferroptosis was established and confirmed to be a valuable independent predictor for overall survival in clear cell renal cell carcinoma. Enrichment analysis showed a correlation with iron metabolism and tumor immunity in ccRCC.
Article
Biochemistry & Molecular Biology
Lini Jin, Binfeng Yu, Hongju Wang, Lingling Shi, Jingjuan Yang, Longlong Wu, Cui Gao, Hong Pan, Fei Han, Weiqiang Lin, En Yin Lai, Yong-Fei Wang, Yi Yang
Summary: This study demonstrates that STING plays a critical role in ferroptosis during ischemic acute kidney injury. It facilitates NCOA4-mediated ferritinophagy, leading to iron overload and exacerbating renal dysfunction.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Multidisciplinary Sciences
Stanley Gutiontov, William Tyler Turchan, Liam F. Spurr, Sherin J. Rouhani, Carolina Soto Chervin, George Steinhardt, Angela M. Lager, Pankhuri Wanjari, Renuka Malik, Philip P. Connell, Steven J. Chmura, Aditya Juloori, Philip C. Hoffman, Mark K. Ferguson, Jessica S. Donington, Jyoti D. Patel, Everett E. Vokes, Ralph R. Weichselbaum, Christine M. Bestvina, Jeremy P. Segal, Sean P. Pitroda
Summary: The study found that the loss of CDKN2A function is associated with poorer clinical outcomes in advanced NSCLC patients treated with ICB, even in high PD-L1 and high TMB tumors. This novel finding should be further validated and may serve as a potential therapeutic target.
SCIENTIFIC REPORTS
(2021)
Article
Surgery
Konjeti R. Sekhar, Sriram Cyr, Naira Baregamian
Summary: This study examines the differential effects of GPX4 inhibitors on thyroid cancer cells and 3D spheroid models, and finds that the inhibitors can induce ferroptosis, increase DNA damage, and have different responses in different cell models. Our study is the first to determine the differential effects of GPX4 inhibitors on thyroid cancer cells with diverse mutational signatures.
WORLD JOURNAL OF SURGERY
(2023)
Review
Cell Biology
Kai Chen, Xiaobing Jiang, Moxin Wu, Xianming Cao, Wendai Bao, Ling-Qiang Zhu
Summary: Ferroptosis is considered as a distinct mechanism in the pathogenesis of Alzheimer's disease, with involvement in key features of AD pathology. Inhibitors of ferroptosis show potential clinical benefits in both AD patients and mice models.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Bumin Xie, Yuan Guo
Summary: Ferroptosis is a newly identified form of regulated cell death characterized by iron-dependent accumulation of lipid reactive oxygen species, closely related to various diseases and tumor treatment. Noncoding RNAs play a crucial role in regulating the molecular mechanism of ferroptosis in tumor cells.
CELL DEATH DISCOVERY
(2021)
Article
Multidisciplinary Sciences
Jinliang Gao, Tao Luo, Jinke Wang
Summary: A therapy based on gene interference-enhanced ferroptosis was developed, utilizing iron nanoparticles to induce significant cell death in cancer cells while having little effect on normal cells. The combination of iron nanoparticles and gene interference led to tumor growth inhibition and durable cure in mice, showing promising results for potential cancer treatment strategies.
NATURE COMMUNICATIONS
(2021)
Review
Plant Sciences
Ayelen Mariana Distefano, Gabriel Alejandro Lopez, Nicolas Setzes, Fernanda Marchetti, Maximiliano Cainzos, Milagros Cascallares, Eduardo Zabaleta, Gabriela Carolina Pagnussat
Summary: Regulated cell death, including ferroptosis, plays crucial roles in plant developmental programs and maintaining organism homeostasis in response to adverse environments. Ferroptosis in plants shares common characteristics with other systems, such as the accumulation of lipid reactive oxygen species, but is influenced by specific metabolic pathways and compartmentalization. This complexity adds layers to understanding the role of ferroptosis in plants.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Agriculture, Multidisciplinary
Meitong Liu, Jing Lu, Yuelin Chen, Shengzhuo Zhang, Jiakang Guo, Shuang Guan
Summary: This study explored the connection between sodium sulfite and ferroptosis, a form of iron-dependent cell death. The researchers observed abnormal expression of a ferroptosis marker protein, suggesting that sodium sulfite caused ferroptosis in vivo. Further investigation revealed that sodium sulfite led to an overproduction of mtROS in cells. This mtROS caused an increase in lysosomal membrane permeability, resulting in iron efflux and ultimately leading to ferroptosis. This study provides a new mechanism for the hepatotoxicity of sodium sulfite and contributes to the risk assessment of sodium sulfite as a food additive.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Review
Immunology
Siyuan Chang, Mengshi Tang, Bikui Zhang, Daxiong Xiang, Fen Li
Summary: This review summarized the relevant research on ferroptosis in various inflammatory arthritis and focused on the relationship between rheumatoid arthritis (RA) and ferroptosis. Iron overload, lipid peroxidation, and mitochondrial dysfunction associated with ferroptosis were found in patients with RA and animal models. In addition, some anti-rheumatic drugs showed modulating effects on ferroptosis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Physics, Applied
Hiromasa Tanaka, Shogo Matsumura, Kenji Ishikawa, Hiroshi Hashizume, Masafumi Ito, Kae Nakamura, Hiroaki Kajiyama, Fumitaka Kikkawa, Mikako Ito, Kinji Ohno, Yasumasa Okazaki, Shinya Toyokuni, Masaaki Mizuno, Masaru Hori
Summary: Ethanol production and cell growth in budding yeast were found to be enhanced by direct plasma irradiation, not by indirect plasma irradiation. This enhancement was attributed to the increased glucose consumption and elevated glycolytic activity in yeast induced by direct plasma irradiation.
JAPANESE JOURNAL OF APPLIED PHYSICS
(2022)
Editorial Material
Biochemistry & Molecular Biology
Shinya Toyokuni, Yingyi Kong, Danyang Mi
Summary: This commentary discusses a highly cited paper by Robert L Heath and Lester Packer, published in the Archives of Biochemistry and Biophysics in 1968. The paper reveals that isolated chloroplasts undergo cyclic peroxidation of tri-unsaturated fatty acids when exposed to visible light, contributing to the concept of electron transfer reactions.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Oncology
Zhen Cheng, Shinya Akatsuka, Guang Hua Li, Kiyoshi Mori, Takashi Takahashi, Shinya Toyokuni
Summary: The study found that A/J strain mice have higher susceptibility to Fe-NTA-induced renal cell carcinoma due to differential expression of genes related to ferroptosis and iron metabolism. A/J mice maintained higher levels of glutathione peroxidase 4 and xCT (SLC7A11) expression after Fe-NTA treatment, leading to lower lipid peroxidation levels.
Article
Biochemistry & Molecular Biology
Yasumasa Okazaki, Kanako Sasaki, Nanami Ito, Hiromasa Tanaka, Ken-Ichiro Matsumoto, Masaru Hori, Shinya Toyokuni
Summary: Non-thermal plasma (NTP) devices play an important role in medical applications, promoting blood coagulation, eliminating infections, facilitating wound healing, and suppressing cancer cell growth. Research has shown that the oxidation reactions between metal ions and biomolecules can be efficiently attenuated by reduced and oxidized glutathione.
FREE RADICAL RESEARCH
(2022)
Article
Pathology
Lin Yue, Yaguang Luo, Li Jiang, Yoshitaka Sekido, Shinya Toyokuni
Summary: Poly (rC)-binding proteins 1 and 2 (PCBP1/2) play an important role in mesothelial cells and malignant mesothelioma (MM) by conferring resistance to ferroptosis. Knockdown of PCBP2 decreases the expression of transferrin receptor 1 (TfR1) and ferritin heavy chain (FTH), inhibits cell proliferation, and increases sensitivity to ferroptosis in MM cells.
PATHOLOGY INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Yingyi Kong, Shinya Akatsuka, Yashiro Motooka, Hao Zheng, Zhen Cheng, Yukihiro Shiraki, Tomoji Mashimo, Tatsuhiko Imaoka, Shinya Toyokuni
Summary: Mutation in the BRCA1 gene increases the risk of cancer in both females and males, with deficiency in DNA repair hypothesized as a responsible mechanism. However, there is insufficient data on the mutation spectra of cancers in BRCA1 mutation carriers and the mechanisms driving carcinogenesis. In this study, a rat model of BRCA1 haploinsufficiency was used to investigate renal carcinogenesis. The results showed that oxidative stress via excess iron is a major driving force for carcinogenesis in BRCA1 haploinsufficiency, suggesting it as a potential target for cancer prevention and therapeutics.
Article
Oncology
Yaguang Luo, Shinya Akatsuka, Yashiro Motooka, Yingyi Kong, Hao Zheng, Tomoji Mashimo, Tatsuhiko Imaoka, Shinya Toyokuni
Summary: Malignant mesothelioma is caused by local iron accumulation, oxidative DNA damage, and genomic alterations in the mesothelium. A recent study found that asbestos exposure in male rats with a BRCA1 mutation promotes the development of mesothelial tumors, suggesting that asbestos exposure may increase the risk of mesothelioma in male patients with BRCA1 mutations.
Article
Oncology
Fumiya Ito, Katsuhiro Kato, Izumi Yanatori, Yuki Maeda, Toyoaki Murohara, Shinya Toyokuni
Summary: Research on organoids of malignant mesothelioma helps predict chemotherapy response. The study found that the organoid system of malignant mesothelioma simulated the original tissue and showed increased sensitivity to cisplatin compared to 2D cultures. This highlights the importance of using organoids to study strategies to overcome cisplatin resistance.
Article
Nanoscience & Nanotechnology
Shin-Ichiro Yamaguchi, Qilin Xie, Fumiya Ito, Kazuki Terao, Yoshinobu Kato, Miki Kuroiwa, Satoshi Omori, Hideo Taniura, Kengo Kinoshita, Takuya Takahashi, Shinya Toyokuni, Kota Kasahara, Masafumi Nakayama
Summary: This study reveals the importance of aromatic clusters in the receptor extracellular loop in recognizing carbon nanotubes and suggests inhibiting the Syk signalling pathway as a potential treatment for inflammation. In silico screening identified the receptors Siglec-5 and Siglec-14 as the ones recognizing carbon nanotubes. Molecular dynamics simulations showed stable association between aromatic residues on Siglec-5 and carbon nanotubes. Further experiments demonstrated that Siglec-14 mediates the phagocytosis of CNTs and induces proinflammatory responses, which can be blocked by a Syk inhibitor.
NATURE NANOTECHNOLOGY
(2023)
Editorial Material
Oncology
Yasumasa Okazaki, Keisuke Hino
Review
Biochemistry & Molecular Biology
Shinya Toyokuni, Hao Zheng, Yingyi Kong, Kotaro Sato, Kae Nakamura, Hiromasa Tanaka, Yasumasa Okazaki
Summary: Plasma, as the fourth state of matter, has the potential for biological and medical applications. The exposure of non-thermal plasma to water can induce oxidative stress and cause higher damage to cancer cells compared to non-tumorous cells. The cell death mode depends on the type of cancer. Thus, non-thermal plasma can be used as an additional cancer therapy in somatic cavities or surgical margins.
FREE RADICAL RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Hiromasa Tanaka, Masaaki Mizuno, Kenji Ishikawa, Camelia Miron, Yasumasa Okazaki, Shinya Toyokuni, Kae Nakamura, Hiroaki Kajiyama, Masaru Hori
Summary: Low-temperature plasma (LTP) has been widely used in life science. LTP can be used to irradiate water, medium, and Ringer's solutions to produce plasma-activated solutions, which contain chemical compounds produced by reactions among LTP, air, and solutions. Reactive oxygen and nitrogen species (RONS) are major components in plasma-activated solutions, and recent studies have shown the production of plasma-activated organic compounds in plasma-activated Ringer's lactate solution (PAL). In vitro and in vivo studies have demonstrated the anti-tumor effects of PAL on cancers, and biochemical analyses have revealed the intracellular molecular mechanisms of cancer cell death by PAL.
FREE RADICAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Yasumasa Okazaki, Nanami Ito, Hiromasa Tanaka, Masaru Hori, Shinya Toyokuni
Summary: Non-thermal plasma (NTP) at near-physiological temperatures can generate reactive oxygen species (ROS) and reactive nitrogen species (RNS), which have potential applications in blood coagulation, wound healing, disinfection, and selective cancer cell death. However, the stoichiometric quantification of NTP-induced ROS in the liquid phase is still unclear, and the interaction between NTP-induced ROS and biomolecules needs further investigation.
FREE RADICAL RESEARCH
(2022)
Meeting Abstract
Oncology
Yingyi Kong, Yashiro Motooka, Shinya Akatsuka, Tomoji Mashimo, Tatsuhiko Imaoka, Shinya Toyokuni
Meeting Abstract
Oncology
Shinya Akatsuka, Li Jiang, Asmaa Elzawahry, Mamoru Kato, Yukari Totsuka, Yasuhito Arai, Tatsuhiro Shibata
