Article
Biotechnology & Applied Microbiology
Jordi Tronchoni, Ramon Gonzalez, Andrea M. Guindal, Elena Calleja, Pilar Morales
Summary: The study revealed significant diversity in acetic acid yield among different S. cerevisiae strains under aerobic conditions, with some strains showing very low volatile acidity. Ethanol yields were lower under aerobic than under anaerobic conditions for all strains, and acetic acid levels were low for two of them. This research paves the way for simplified aerobic fermentation protocols aimed at reducing the alcohol content of wines.
Review
Biotechnology & Applied Microbiology
Wilson Jose Fernandes Lemos Junior, Vanessa Sales de Oliveira, Andre Fioravante Guerra, Alessio Giacomini, Viviana Corich
Summary: Starmerella bacillaris strains are known for their osmotolerance, glycerol production, and potential as a biocontrol agent. They release important molecules during fermentation and have promising applications in low-alcohol beverages.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Charlotte Vion, Emilien Peltier, Margaux Bernard, Maitena Muro, Philippe Marullo
Summary: Breeding programs taking advantage of the diversity in natural Saccharomyces cerevisiae yeast strains are used to select individuals with outlier properties for winemaking, improving malic acid consumption capacity. Selected individuals greatly reduce malic acid content, show robustness to the environment, and accelerate malolactic fermentations by Oenococcus oeni.
Article
Biotechnology & Applied Microbiology
Maarten L. De Mol, Victoria Marcoen, Isabelle Maryns, Nico Snoeck, Joeri J. Beauprez, Sofie L. De Maeseneire, Wim K. Soetaert
Summary: This study assessed the impact of genomic instability on the fermentation performance of engineered yeasts, and proposed the use of novel host-specific parameters to standardize strain robustness and evaluate potential improvements. By leveraging these parameters, it becomes possible to predict strain behavior prior to large-scale production, enhancing the economic viability of microbial fermentation processes and bridging the gap between laboratory findings and industrial applications.
FERMENTATION-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Anita Oberholster, Yan Wen, Sandra Dominguez Suarez, Jesse Erdmann, Raul Cauduro Girardello, Arran Rumbaugh, Bishnu Neupane, Charles Brenneman, Annegret Cantu, Hildegarde Heymann
Summary: There is an increase in volatile phenols in wine made with smoke-impacted grapes. Mitigation strategies during red wine fermentation have limited impact on the extraction of smoke-taint markers and sensory characteristics.
Article
Multidisciplinary Sciences
Elena C. Pulido-Mateos, Jacob Lessard-Lord, Denis Guyonnet, Yves Desjardins, Denis Roy
Summary: This study revealed the metabolic and genomic features of TanA+L. plantarum strains and developed a screening technique. The results showed that these strains have the ability to resist and transform gallotannins.
SCIENTIFIC REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Andrea M. Guindal, Ramon Gonzalez, Jordi Tronchoni, Jorik S. Roodink, Pilar Morales
Summary: The use of yeast respiratory metabolism to increase ethanol content in wine is hindered by acetic acid overproduction. Directed evolution was performed to recover yeast strains with reduced acetic acid production, but the results were not satisfactory.
Article
Biotechnology & Applied Microbiology
Chun Tang Feng, Xue Du, Josephine Wee
Summary: This study isolated non-Saccharomyces yeasts from Chambourcin must in Pennsylvania vineyards and characterized strains that can tolerate ethanol and sulfite, potentially enhancing flavor complexity and regional characteristics in hybrid wines. Lab-scale fermentations revealed correlations between specific yeast strains and compounds associated with fruity and floral or herbaceous aromas, suggesting an exciting approach to improve wine quality.
FERMENTATION-BASEL
(2021)
Article
Food Science & Technology
Evelyne Timmermans, Ine Langie, An Bautil, Kristof Brijs, Carolien Buve, Ann Van Loey, Ilse Scheirlinck, Roel Van der Meulen, Christophe M. Courtin
Summary: Despite the limited exploration of the natural diversity of yeasts, this study investigates the fermentation characteristics of 23 yeasts from various industries in sweet dough. Significant differences in sugar consumption, metabolite and volatile compound production were observed, and non-conventional yeast strains showed potential for positive aroma compound production in sweet dough.
Article
Biotechnology & Applied Microbiology
Chengzhen Yao, Xiaoqing Hu, Xiaoyuan Wang
Summary: An efficient CRISPR/Cas9-assisted genomic editing system was developed for Corynebacterium glutamicum, achieving a 95% editing efficiency in ATCC 13032 strain. The entire editing process can be completed in 8-9 days, making it more time-saving compared to previous approaches.
Article
Biotechnology & Applied Microbiology
Keiji Nishida, Akihiko Kondo
Summary: CRISPR-Cas system offers various modes for DNA editing in metabolic engineering, with consideration of host-dependent repair pathways. The versatility of CRISPR system has led to derivative technologies to complement nuclease-based editing, avoiding cytotoxicity, especially in microorganisms.
METABOLIC ENGINEERING
(2021)
Article
Microbiology
Sarah M. Doore, Sundharraman Subramanian, Nicholas M. Tefft, Renato Morona, Michaela A. TerAvest, Kristin N. Parent
Summary: The study describes the genome of S. flexneri strain PE577 and its phenotypic differences compared to model strain 2457T. PE577 lacks functional CRISPR and restriction-modification systems, exhibiting lower transformation efficiency, higher oxygen sensitivity, altered carbon metabolism, and greater susceptibility to lytic bacteriophages. Understanding these differences could help in developing vaccines and therapeutics to combat Shigella infection.
JOURNAL OF BACTERIOLOGY
(2021)
Review
Biology
Xiunan Yi, Hal S. Alper
Summary: The metabolic capacity of yeast strains can vary within the same species, which affects their performance in metabolic engineering. Understanding the characteristics of different strains is crucial for successful metabolic engineering applications.
Article
Microbiology
Marie-Jose Ayoub, Jean-Luc Legras, Pierre Abi-Nakhoul, Huu-Vang Nguyen, Rachad Saliba, Claude Gaillardin
Summary: 296 Saccharomyces cerevisiae isolates from naturally fermenting grape musts in Lebanon were analyzed using interdelta fingerprinting. A diverse natural oenological flora in Lebanon was observed, with evidence of lineage dominance and circulation of strains within wineries and vicinal areas. The Lebanese flora showed interrelatedness at microsatellite loci compared to worldwide communities, and 21 indigenous strains were tested for winemaking potential, with 7 passing pre-selection criteria and 2 identified as good candidates pending pilot-scale assays.
Review
Biotechnology & Applied Microbiology
Jennifer M. Gardner, Lucien Alperstein, Michelle E. Walker, Jin Zhang, Vladimir Jiranek
Summary: The microbial population in wines plays a crucial role in determining their quality. Winemakers can use both indigenous and purposefully inoculated yeasts to achieve desired aromas, flavors, and structure in the fermented grape juice. Having a diverse toolbox of microbes helps winemakers adapt to different grape qualities and overcome fermentation challenges. This article reviews the commercially available yeast strains and explores the advancements in strain improvement technologies. It also discusses the current use of microbes in the Australian wine industry and the regulations and sentiment surrounding genetically modified organisms in winemaking.
FEMS YEAST RESEARCH
(2023)
Article
Microbiology
Anna Kallistova, Yury Nikolaev, Vladimir Grachev, Alexey Beletsky, Evgeny Gruzdev, Vitaly Kadnikov, Alexander Dorofeev, Julia Berestovskaya, Anna Pelevina, Ivar Zekker, Nikolai Ravin, Nikolai Pimenov, Andrey Mardanov
Summary: The addition of formate promotes dissimilatory nitrate reduction and stimulates the growth of Ca. Jettenia spp., while the addition of folate stimulates the daily nitrogen removal rate. Additionally, formate leads to spatial separation of the two anammox species.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Andrey L. Rakitin, Shahjahon Begmatov, Alexey V. Beletsky, Dmitriy A. Philippov, Vitaly V. Kadnikov, Andrey V. Mardanov, Svetlana N. Dedysh, Nikolai V. Ravin
Summary: In this study, we investigated the microbial diversity in neighboring raised strings and submerged flarks in a mire site in European North Russia. The microbial communities in the two structures showed distinct patterns, with raised strings dominated by Alpha- and Gammaproteobacteria, and submerged flarks dominated by methanogenic lineages.
Article
Infectious Diseases
Andrey L. Rakitin, Yulia K. Yushina, Elena V. Zaiko, Dagmara S. Bataeva, Oksana A. Kuznetsova, Anastasia A. Semenova, Svetlana A. Ermolaeva, Aleksey V. Beletskiy, Tat'yana V. Kolganova, Andrey V. Mardanov, Sergei O. Shapovalov, Timofey E. Tkachik
Summary: This study investigated the occurrence of antimicrobial resistance among Salmonella strains isolated from food products. Multiple serovars of Salmonella with resistance to commonly used antibiotics were identified. Genomic analysis revealed the presence of antibiotic resistance genes within integrons in plasmids or chromosomal islands. Pathogenicity islands were also found in the genomes of the sequenced strains. The emergence of multidrug-resistant Salmonella strains highlights the need for further research on transmission pathways and monitoring of these microorganisms' distribution.
Article
Microbiology
Aleksandr G. Bulaev, Aleksandra Kanygina, Andrey Mardanov
Summary: This study reports the draft genome sequence of Acidiplasma aeolicum strain V1(T), isolated from a hydrothermal pool in Vulcan Island, Italy. The genome is 1.8 Mbp long with a GC content of 34%. The genome sequence shares close similarity with other known strains of the Acidiplasma genus.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Microbiology
Olga V. Karnachuk, Alexey V. Beletsky, Andrey L. Rakitin, Olga P. Ikkert, Marat R. Avakyan, Viacheslav S. Zyusman, Andrei Napilov, Andrey V. Mardanov, Nikolai V. Ravin
Summary: Sulphate-reducing bacteria, particularly Desulfovibrio, play a key role in the production of H2S in swine waste. Desulfovibrio vulgaris strain L2, a model organism for sulphate reduction studies, can utilize animal farming supplements as electron acceptors for H2S production. The genome sequencing of strain L2 revealed the presence of antibiotic resistance genes (ARG) and genes conferring resistance to mercury. These resistance genes are likely acquired through horizontal gene transfer from other bacteria. The presence of ARGs on mobile genetic elements suggests strain L2 may contribute to the transfer of antimicrobial resistance determinants between the gut microbiota and environmental microbial communities.
Article
Biology
Vitaly V. Kadnikov, Andrey V. Mardanov, Alexey V. Beletsky, Olga V. Karnachuk, Nikolai V. Ravin
Summary: The research focused on prokaryotic communities near hot gas vents in an open quarry impacted by underground coal fire. The dominant species were identified as spore-forming Firmicutes, which were capable of obtaining energy from hydrogen and carbon monoxide in coal gases. The analysis also revealed the genome of a previously uncultured bacterium, which was found to be rod-shaped and potentially involved in scavenging and fermenting organics produced by autotrophic Firmicutes.
Article
Biotechnology & Applied Microbiology
Andrey V. Mardanov, Eugeny V. Gruzdev, Alexey V. Beletsky, Elena V. Ivanova, Maksim Yu. Shalamitskiy, Tatiana N. Tanashchuk, Nikolai V. Ravin
Summary: The genome sequencing of industrial flor yeast strain I-329 and its derivatives from the winery Magarach revealed genetic stability under biological wine aging. The analysis of 18S rRNA gene and metagenomes showed that the batch aging process had a higher proportion of bacteria in the flor velum compared to the continuous process.
FERMENTATION-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Nikolai V. Ravin, Maria S. Muntyan, Dmitry D. Smolyakov, Tatyana S. Rudenko, Alexey V. Beletsky, Andrey V. Mardanov, Margarita Yu. Grabovich
Summary: Two metagenome-assembled genomes related to the family Thiotrichaceae were obtained from a sulfide-rich thermal spring. They were identified as new species and genera, showing metabolic versatility and adaptability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Nikolai Pimenov, Yuri Nikolaev, Vladimir Grachev, Anna Kallistova, Alexander Dorofeev, Yuriy Litti, Evgeny Gruzdev, Alexey Beletsky, Nikolai Ravin, Andrey Mardanov
Summary: This study investigated the impact of sulfide on nitrogen transformation and removal efficiency in the Anammox process. The results showed that sulfide had little effect on nitrogen removal efficiency during ammonium oxidation and anammox, but it had a certain inhibitory effect on nitrite oxidation. Furthermore, sulfide had different effects on the growth rate of different types of nitrifying bacteria. However, further research is needed to understand these findings.
Article
Environmental Sciences
Alexander Dorofeev, Anna Pelevina, Yuri Nikolaev, Yulia Berestovskaya, Evgeny Gruzdev, Andrey Mardanov, Nikolai Pimenov
Summary: This study investigated the substrate range of Ca. Accumulibacter by measuring its oxygen uptake rates (OUR) with 17 different organic substrates. The results showed that Ca. Accumulibacter preferred tryptone, volatile fatty acids, succinate, pyruvate, and amino acids as substrates. The possible pathways for substrate transport and metabolism by Ca. Accumulibacter were discussed using stoichiometric data and metagenomic analysis.
Article
Microbiology
Nikolai V. Ravin, Dmitry D. Smolyakov, Nikita D. Markov, Alexey V. Beletsky, Andrey V. Mardanov, Tatyana S. Rudenko, Margarita Yu. Grabovich
Summary: The study proposes the use of tRNA(Ile)-lysidine synthase (tilS) and beta subunit of RNA polymerase (rpoB) genes as phylogenetic markers for the genus Thiothrix. Taxonomic analysis using these genes showed that MK1 and WS belong to Thiothrix lacustris, while DNT52, DNT53, and H33 belong to Thiothrix subterranea. High-throughput sequencing of tilS gene fragments revealed the presence of new Thiothrix species.
Review
Food Science & Technology
Maksim Yu. Shalamitskiy, Tatiana N. Tanashchuk, Sofia N. Cherviak, Egor A. Vasyagin, Nikolai V. Ravin, Andrey V. Mardanov
Summary: Ethyl carbamate, a probable human carcinogen, is found in fermented foods and alcoholic beverages. This review summarizes its formation mechanisms, impact on human health, and methods used to minimize its content.
Article
Biology
Vitaly V. Kadnikov, Nikolai V. Ravin, Diyana S. Sokolova, Ekaterina M. Semenova, Salimat K. Bidzhieva, Alexey V. Beletsky, Alexey P. Ershov, Tamara L. Babich, Marat R. Khisametdinov, Andrey V. Mardanov, Tamara N. Nazina
Summary: The microbial community composition of petroleum reservoirs with high-salinity formation water in Tatarstan, Russia was studied using metagenomic and culture-based approaches. The results showed that different microbial species play different roles in the reservoirs, and it is necessary to consider both the activation of fermentative bacteria and the suppression of sulfidogenesis when choosing MEOR technologies.
Article
Microbiology
Andrey V. Mardanov, Alexey V. Beletsky, Egor A. Vasyagin, Tatiana N. Tanashchuk, Maxim Yu. Shalamitskiy, Nikolai V. Ravin
Summary: This study reports the complete circular genome sequence of the important lactic acid bacteria Oenococcus oeni strain K19-3, which is capable of carrying out malolactic fermentation and improving the organoleptic properties of wine. It was isolated from red grape must at Crimean wineries.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Microbiology
Aleksandr G. Bulaev, Aleksandra V. Kanygina, Andrey V. Mardanov
Summary: Here, we report the draft genome sequence of Acidiplasma cupricumulans Strain BH2(T), a type strain isolated from a pregnant leachate solution of an industrial-scale chalcocite bioleach heap in Monywa, Myanmar. The genome is 1.7 Mbp in length with a GC content of 34.2%.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
