Article
Plant Sciences
Alecia Biel, Morgan Moser, Norman R. Groves, Iris Meier
Summary: The study showed that SINE1 and SINE2 play important roles in actin pattern changes during ABA-induced stomatal closure, but at different time steps. External Ca2+ can partially rescue the mutant effects. Nuclear Ca2+ oscillations during ABA-induced stomatal closure were observed for the first time, and were disrupted in the mutant. Vacuolar fragmentation was impaired in the mutants and could be partially rescued by F-actin depolymerization.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Liufeng Dai, Baojie Wang, Ting Wang, Etienne H. Meyer, Valentin Kettel, Natalie Hoffmann, Heather E. McFarlane, Shalan Li, Xuna Wu, Kelsey L. Picard, Patrick Giavalisco, Staffan Persson, Yi Zhang
Summary: Energy is crucial for cellular functions, but how actin dynamics are regulated by ATP levels in plant cells is not well understood. This study reveals an important role for TORC1 in coordinating ATP homeostasis and actin dynamics in plant cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Muhammad Bilal Ahmed, Salman Ul Islam, Young Sup Lee
Summary: PRP4 inhibits melanin production, blocks calcium influx through desensitization of CaSR, and modulates the actin cytoskeleton through downregulation of the AC-cAMP pathway, collectively leading to the promotion of skin carcinogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Jing Gao, Fumihiko Nakamura
Summary: This review article introduces actin-associated proteins (AAPs) and their roles in regulating cell movement, shape change, division, organelle localization, and trafficking. The article lists all discovered AAPs and allows sorting based on various criteria. It also provides links to databases for accessing detailed information about protein structures, expression levels, mutations, and pathology. Additionally, small molecules targeting actin and AAPs with potential for treating diseases are listed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Nicolas E. Figueroa, Peter Franz, Marcin Luzarowski, Federico Martinez-Seidel, Juan C. Moreno, Dorothee Childs, Aleksandra Ziemblicka, Arun Sampathkumar, Tonni Grube Andersen, Georgios Tsiavaliaris, Monika Chodasiewicz, Aleksandra Skirycz
Summary: By using thermal proteome profiling, we identified 51 proteins that interact with 3',5'-cAMP. Further investigation revealed that 3',5'-cAMP can affect plant growth by regulating the organization of actin cytoskeleton. Our study provides a specific resource, the 3',5'-cAMP interactome, and functional insights into 3',5'-cAMP-mediated regulation in plants.
Article
Pharmacology & Pharmacy
Coralie Mergault, Fanny Lisee, Victor Tiroille, Melia Magnien, Christelle Parent, Woodys Lenga Mabonda, Damien Sizaret, Madeleine Jaillet, Bruno Crestani, Sylvain Marchand-Adam, Laurent Plantier
Summary: The study found that the expression of the Arp2/3 complex was increased in profibrotic environments in vitro and in vivo. Inhibition of the Arp2/3 complex repressed ACTA2 and COL1 expression and blocked the Akt/phospho-GSK3 beta/beta-catenin/MRTF-A pathway in lung fibroblasts. CK666 demonstrated antifibrotic properties in the lung in vivo.
BRITISH JOURNAL OF PHARMACOLOGY
(2022)
Article
Cell Biology
Samantha M. Richter, Lilyanna C. Massman, Dennis J. Stuehr, Elizabeth A. Sweeny
Summary: NADPH oxidase 5 (NOX5) is a transmembrane oxidative signaling enzyme that produces superoxide in response to intracellular calcium flux. It is involved in physiological processes and human disease, but its signaling pathways and targets of oxidative modifications remain poorly understood. This study demonstrates that NOX5 and actin interact in the cell and modulate each other's activity. Changes in actin dynamics affect NOX5 superoxide production, and NOX5 oxidatively modifies actin. Knockdown of NOX5 impairs cell migration. These findings reveal an important link between actin dynamics and oxidative signaling through NOX5.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Micaela Boiero Sanders, Christopher P. Toret, Audrey Guillotin, Adrien Antkowiak, Thomas Vannier, Robert C. Robinson, Alphee Michelot
Summary: The use of different actin isoforms in eukaryotic cells and the molecular mechanisms of their segregation into distinct networks are poorly understood. By using yeast as a model, researchers found that the expression of heterologous actin causes significant reorganization of the actin cytoskeleton. However, the expression of two heterologous actin variants, each specialized in assembling a different network, can rescue cytoskeletal organization and increase resistance to external perturbation.
Article
Multidisciplinary Sciences
Joseph Puccini, Jia Wei, Liang Tong, Dafna Bar-Sagi
Summary: Macropinocytosis is a nonselective endocytosis process that facilitates the uptake of extracellular fluid-phase cargoes. Tumor cells exploit this process to acquire macromolecules for cell growth and proliferation under nutrient-limiting conditions. This study reveals that the enzyme ATP citrate lyase (ACLY) is a key regulator of macropinocytosis, and it is involved in the actin cytoskeleton. The findings demonstrate a mechanism that links regional acetyl-CoA availability with dynamic protein acetylation, supporting the spatial and temporal modulation of membrane-cytoskeletal interactions required for macropinocytosis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biology
Daan Vorselen, Sarah R. Barger, Yifan Wang, Wei Cai, Julie A. Theriot, Nils C. Gauthier, Mira Krendel
Summary: The study reveals that during macrophage phagocytosis of antibody-opsonized targets, there is a prominent constriction driven by Arp2/3-mediated assembly of discrete actin protrusions. Contractile myosin-II activity contributes to late-stage phagocytic force generation and target ingestion. Observations of partial target eating attempts and sudden target release suggest that constriction may play a critical role in resolving complex in vivo target encounters.
Article
Urology & Nephrology
Anne K. Muehlig, Johanna Steingroever, Hannah S. Heidelbach, Madelaine Wingerath, Wiebke Sachs, Irm Hermans-Borgmeyer, Catherine Meyer-Schwesinger, Hoon Young Choi, Beom Jin Lim, Christian Patry, Georg Friedrich Hoffmann, Nicole Endlich, Katharina Bracke, Mariella Weiss, Andreas H. Guse, Moritz Lasse, Markus M. Rinschen, Fabian Braun, Tobias B. Huber, Victor G. Puelles, Claus Peter Schmitt, Jun Oh
Summary: Activation of podocyte CaSR regulates key podocyte functions and reduces toxin-induced proteinuria and glomerular damage.
KIDNEY INTERNATIONAL
(2022)
Review
Pharmacology & Pharmacy
V. B. Dugina, G. S. Shagieva, P. B. Kopnin
Summary: Cytoplasmic actin isoforms play important roles in cellular processes and their imbalances are linked to pathology. Understanding the underlying mechanisms is relevant for both basic research and clinical applications. Study of actin diversity and function in cancer may contribute to treatment strategies.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Cell Biology
Yiju Wei, Wei Li
Summary: The Hippo pathway is a critical signaling network that regulates organ development and tissue homeostasis. Recent studies have identified calcium as a potential messenger in transducing signals to the main signaling machinery.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Neurosciences
Tanya L. Brown, Hirokazu Hashimoto, Lisbet T. Finseth, Teresa L. Wood, Wendy B. Macklin
Summary: The actin regulator PAK1 promotes oligodendrocyte morphologic changes and myelin production, influencing communication within the CNS.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Sharad V. Jaswandkar, Kalpana S. Katti, Dinesh R. Katti
Summary: This study investigates the impact of ADF/cofilin on actin filaments using molecular dynamics simulations. The results show that the binding of ADF/cofilin modifies the conformation and alignment of actin filaments locally, leading to filament severing at the boundaries. The findings provide important insights into the cofilin-mediated actin dynamics.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)