4.5 Article

Synergistic effect of TEMPO-coated TiO2 nanorods for PDT applications in MCF-7 cell line model

期刊

SAUDI JOURNAL OF BIOLOGICAL SCIENCES
卷 27, 期 12, 页码 3199-3207

出版社

ELSEVIER
DOI: 10.1016/j.sjbs.2020.09.027

关键词

TEMPO; Titanium dioxide nanorods (TiO2 NRs); Photodynamic therapy (PDT); MCF-7 cell line

类别

资金

  1. Higher Education Commission Pakistan under NRPU Project Grant [8056/Punjab/N RPU/RD/HEC/2017]
  2. Deanship of Scientific Research at King Saud University [RGP-293]

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This study focuses on the synthesis, characterization, and assessment of the synergistic effect of 2,2,6,6, tetramethylpiperidine-N-oxyl (TEMPO)-coated titanium dioxide nanorods (TiO2 NRs) for photodynamic therapy (PDT). Firstly, TiO2 NRs were synthesized by the sol-gel technique. Then, TEMPO was grafted on TiO2 NRs with the aid of oxoammonium salts. Next, the final product was characterized by applying manifold characterization techniques. X-ray diffraction was used to perform crystallographic analysis; transmission electron microscopy (TEM) was used to conduct morphological analysis; Fourier transform infrared (FTIR) and Raman spectra were recorded to perform molecular fingerprint analysis. Furthermore, experimental and empirical modeling was performed to confirm the suitability of as-prepared samples for PDT applications using (MCF-7 cell line) Human Breast Cancer cell line. Our results revealed that bare TiO2 NRs did not exhibit a significant response for therapeutic applications compared to TEMPO conjugated TiO2 NRs in the dark; however, they exhibited a prominent response for the PDT application under UV-A light. Therefore, it is concluded that TEMPO-coated TiO2 NRs shows the synergistic response for therapeutic approach under UV-A light irradiation. In addition, TEMPO capped TiO2 nanorods not only overcome the multidrug resistance (MDR) hindrance but also exhibit excellent response for cancer cell (MCF-7 cells) treatment only under UV light irradiation via PDT. It is expected that the proposed TiO2 NRs + TEMPO nanocomposite, which is suitable for PDT treatment, may be essential for photodynamic therapy. (C) 2020 The Author(s). Published by Elsevier B.V. on behalf of King Saud University.

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