Article
Biochemical Research Methods
Youngtaek Kim, Jiyoung Song, Younggyun Lee, Sunghyun Cho, Suryong Kim, Seung-Ryeol Lee, Seonghyuk Park, Yongdae Shin, Noo Li Jeon
Summary: The newly designed injection molded microfluidic device addresses the inherent limitations of PDMS-manufactured devices and provides a more convenient way to study single-cell behaviors while saving time. The spatiotemporal dynamics in the device were demonstrated through experiments and computational simulations. This easy-to-use plastic microfluidic device promises to open a new approach for investigating the mechanisms of cell behavior at the single-cell level.
Article
Chemistry, Physical
Jingyu Qi, Haibin Zhu, Yijian Li, Xiangyu Guan, Ying He, Guanhua Ren, Qiang Guo, Longqi Liu, Ying Gu, Xuan Dong, Ya Liu
Summary: Cell-cell interaction is an important way for transmitting information and activating the effects of functional cells. Researchers propose a droplet-based microfluidic platform for cell-cell interaction sequencing and screening, which allows for efficient analysis and interpretation of single-cell transcriptomes in cell interactions. This work fills a gap in the current experimental platform for cell-cell interaction research and has significant practical implications in biomedicine.
Article
Biochemical Research Methods
Shujie Yang, Joseph Rufo, Ruoyu Zhong, Joseph Rich, Zeyu Wang, Luke P. P. Lee, Tony Jun Huang
Summary: Acoustic tweezers offer an efficient and contact-free way to manipulate individual cells and particles. Its application in next-generation cellular assays can enhance our understanding of biological systems. With acoustic tweezers, users can conduct a variety of experiments involving trapping, patterning, pairing, and separating single cells in the field of biological sciences.
Review
Chemistry, Analytical
Majood Haddad, Alex N. Frickenstein, Stefan Wilhelm
Summary: Understanding nanoparticle-cell interactions at single-nanoparticle and single-cell resolutions is crucial to improving the design of next-generation nanoparticles for safer, more effective, and more efficient applications in nanomedicine. This review focuses on recent advances in the continuous high-throughput analysis of nanoparticle-cell interactions at the single-cell level, discussing methods such as flow cytometry and inductively coupled plasma mass spectrometry, as well as their intersection in mass cytometry. The review also explores the challenges and opportunities in current single-cell analysis approaches and proposes directions for innovation in high-throughput analysis of nanoparticle-cell interactions.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Mahmut Kamil Aslan, Yun Ding, Stavros Stavrakis, Andrew J. Demello
Summary: We present a portable imaging flow cytometer that combines a smartphone, a small optical framework, and a PDMS-based microfluidic device. The system offers two operational modes for particle/cell analysis: a post-processing mode for particle sizing and a real-time mode for cell/particle classification using machine learning. The flow cytometer is capable of high-resolution imaging and accurate classification using a custom-developed Android application and a machine learning-based algorithm.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Gerjen H. Tinnevelt, Kristiaan Wouters, Geert J. Postma, Rita Folcarelli, Jeroen J. Jansen
Summary: White blood cells play a crucial role in protecting the body from diseases, but can also be related to chronic inflammation, auto immune diseases, or leukemia. High-throughput analytical instruments have been developed to measure multiple proteins on millions of single cells to study the identity and function of different white blood cell types. Multivariate statistics are essential for fully extracting the information-rich biochemistry data. The process involves analyzing the study design, formulating a research question, preparing the data, converting single cells into a cellular distribution, and using clustering methods or models for analysis to differentiate cell (sub)types between groups.
ANALYTICA CHIMICA ACTA
(2021)
Article
Multidisciplinary Sciences
Jonathan Briones, Wilfred Espulgar, Shohei Koyama, Hyota Takamatsu, Eiichi Tamiya, Masato Saito
Summary: This study focuses on achieving high-throughput single cell compartmentalization for quantifying protein expression in single-cell enzymatic assays through the design and optimization of a microfluidic platform. Utilizing COMSOL Multiphysics modeling, optimized parameters for circular microvalves that can close thousands of microchambers at lower sealing pressure were determined. The improvement in microfluidic chip throughput is crucial for applications in single cell analysis, especially in drug discovery and personalized treatment.
SCIENTIFIC REPORTS
(2021)
Review
Biochemical Research Methods
Shuting Xu, Cheng Yang, Xiuping Yan, Huwei Liu
Summary: Mass spectrometry is utilized for single-cell analysis due to its sensitivity and dynamic ranges, with recent advancements focusing on improving automation and throughput to address challenges in manual techniques. Strategies have been developed to enhance information coverage and achieve comprehensive single-cell multiomics data during high throughput detection, providing a promising outlook for single-cell research.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Pengfei Zhang, Aniruddha M. Kaushik, Kuangwen Hsieh, Sixuan Li, Shawna Lewis, Kathleen E. Mach, Joseph C. Liao, Karen C. Carroll, Tza-Huei Wang
Summary: The development of a new cascaded droplet microfluidic platform has addressed the scalability constraint in rapid single-cell AST technologies, demonstrating the potential for generating multiple groups of droplets with custom antibiotic conditions within a single device. This platform has shown promising results in producing clinically useful antibiograms with minimum inhibitory concentrations in approximately 90 minutes for the first antibiotic and 2 minutes for each subsequent antibiotic condition. The platform also demonstrated potential clinical utility in testing clinical isolates and urine specimens against commonly used antibiotics, achieving high categorical agreements with laboratory-based results in a significantly shorter timeframe.
Article
Engineering, Biomedical
Chao Wang, Chunhua Wang, Yu Wu, Jianwei Gao, Yingkuan Han, Yujin Chu, Le Qiang, Jiaoyan Qiu, Yakun Gao, Yanhao Wang, Fangteng Song, Yihe Wang, Xiaowei Shao, Yu Zhang, Lin Han
Summary: This work presents a high-throughput platform for living single-cell multi-index secreted biomarker profiling, combined with machine learning for accurate tumor cell classification, offering new intelligent tools for cancer research and biomedical studies.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Aynur Abdulla, Ting Zhang, Shanhe Li, Wenke Guo, Antony R. Warden, Yufang Xin, Nokuzola Maboyi, Jiatao Lou, Haiyang Xie, Xianting Ding
Summary: In this study, an integrated multifunctional microfluidic system for highly efficient and label-free CTC isolation, enrichment, and single-cell immunoblotting (ieSCI) was developed. The system successfully identified a subgroup of cells that traditional bulk analysis could not detect and demonstrated its clinical application in breast cancer patients.
MICROSYSTEMS & NANOENGINEERING
(2022)
Review
Chemistry, Analytical
Xuhao Luo, Jui-Yi Chen, Marzieh Ataei, Abraham Lee
Summary: Traditional cellular analytical technologies often overlook the individual differences within a cell population, while microfluidic technology allows for precise manipulation of individual cells in small volumes, thus providing a better understanding of cellular heterogeneity.
Article
Chemistry, Analytical
Fengtao Jiang, Nan Xiang
Summary: This paper presents the development of a novel handheld sorter with a multifunction integrated microfluidic chip for point-of-care diagnosis in resource-limited settings. The sorter utilizes a spiral inertial microfluidic channel and flow regulators for size-based cell separation. The prototype of the sorter successfully separates malignant tumor cells from clinical pleural effusions. The device is simple to use, inexpensive, portable, and compact, making it suitable for high-throughput label-free cell separation in resource-limited areas.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Ben Korin, Jun-Jae Chung, Shimrit Avraham, Andrey S. Shaw
Summary: This protocol describes a method for isolating high-quality mouse glomerular cells for high-throughput analysis, which involves direct perfusion of Dynabeads and isolation by magnet to efficiently purify and obtain single glomerular cells with high yield and viability. The balanced representation of different cell types in the glomerulus and high cell viability confirm the efficiency of this method, providing a valuable technique for future studies on glomerular cells in health, injury, and disease.
Review
Biotechnology & Applied Microbiology
Wen-min Zhou, Yan-yan Yan, Qiao-ru Guo, Hong Ji, Hui Wang, Tian-tian Xu, Bolat Makabel, Christian Pilarsky, Gen He, Xi-yong Yu, Jian-ye Zhang
Summary: The inherent heterogeneity of individual cells in cell populations plays significant roles in disease development and progression, with traditional gene profiling methods often masking such differences. Single cell sequencing and microfluidic technologies have emerged as frontiers in research, providing insights into individual cell differences and complex cell populations. The advantages and disadvantages of common high-throughput single cell sequencing technologies are discussed, along with brief illustrations of microfluidic applications in single cell sequencing for cancer and immune system disease diagnosis.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Materials Science, Biomaterials
Zhongliang Jiang, Bingzhao Xia, Ralph McBride, John Oakey
JOURNAL OF MATERIALS CHEMISTRY B
(2017)
Article
Materials Science, Biomaterials
Paige J. LeValley, Ben Noren, Prathamesh M. Kharkar, April M. Kloxin, Jesse C. Gatlin, John S. Oakey
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2018)
Article
Materials Science, Biomaterials
Paige J. LeValley, Ben Noren, Prathamesh M. Kharkar, April M. Kloxin, Jesse C. Gatlin, John S. Oakey
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2018)
Article
Engineering, Biomedical
Zhongliang Jiang, Kun Jiang, Ralph McBride, John S. Oakey
BIOMEDICAL MATERIALS
(2018)
Article
Biophysics
Paige J. LeValley, Mark W. Tibbitt, Ben Noren, Prathamesh Kharkar, April M. Kloxin, Kristi S. Anseth, Mehmet Toner, John Oakey
COLLOIDS AND SURFACES B-BIOINTERFACES
(2019)
Article
Biochemical Research Methods
Zhongliang Jiang, Rajib Shaha, Kun Jiang, Ralph McBride, Carl Frick, John Oakey
IEEE TRANSACTIONS ON NANOBIOSCIENCE
(2019)
Article
Biochemical Research Methods
Benjamin E. Noren, Rajib K. Shaha, Alan T. Stenquist, Carl P. Frick, John S. Oakey
IEEE TRANSACTIONS ON NANOBIOSCIENCE
(2019)
Article
Engineering, Biomedical
Kelly C. Santos Roballo, Subash Dhungana, Zhongliang Jiang, John Oakey, Jared S. Bushman
Article
Materials Science, Biomaterials
Zhongliang Jiang, Kun Jiang, Hangjun Si, Ralph McBride, John Kisiday, John Oakey
Summary: In this study, two microfluidic droplet device designs were used to effectively encapsulate equine mesenchymal stromal cells (MSCs) into microscale hydrogel particles. The length of the linker used influenced the gel formation rate and physical properties of the microgels. Encapsulated cells exhibited high viability and gene expression, and the microgels promoted the migration and proliferation of fibroblasts. This research has the potential to advance tissue engineering and regenerative medicine.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Engineering, Biomedical
Zhongliang Jiang, Rajib Shaha, Ralph McBride, Kun Jiang, Mingchen Tang, Bang Xu, Alexander K. Goroncy, Carl Frick, John Oakey
Article
Biochemical Research Methods
Jitender Bisht, Paige LeValley, Benjamin Noren, Ralph McBride, Prathamesh Kharkar, April Kloxin, Jesse Gatlin, John Oakey
Article
Biochemical Research Methods
Bingzhao Xia, Zhongliang Jiang, Daniel Debroy, Dongmei Li, John Oakey
Review
Biochemical Research Methods
Pei-Ru Chen, Peng-Fei Xia
Summary: Carbon dioxide plays a crucial role in mitigating global climate change and supporting life on Earth. Synthetic biology and metabolic engineering offer a promising approach to enhance CO2 fixation by combining the advantages of autotrophic and heterotrophic organisms. This article reviews the current progress in constructing synthetic CO2 fixation pathways, discusses the underlying design principles and challenges, and provides insights into the future of carbon recycling through engineered synthetic carbon fixation pathways.
CURRENT OPINION IN BIOTECHNOLOGY
(2024)