Article
Pharmacology & Pharmacy
Kristeen Ye Wen Teo, Shipin Zhang, Jia Tong Loh, Ruenn Chai Lai, Hwee Weng Dennis Hey, Kong-Peng Lam, Sai Kiang Lim, Wei Seong Toh
Summary: MSC exosomes promote the polarization of M2-like macrophages through activation of the toll-like receptor signaling pathway and CD73-mediated adenosine production. The production of adenosine activates AKT/ERK-dependent signaling pathways via binding to adenosine receptors A(2A) and A(2B). These findings highlight the critical role of CD73 in mediating M2-like macrophage polarization by MSC exosomes, and have implications for predicting the immunomodulatory potency of MSC exosome preparations.
Article
Immunology
Qiuju Li, Ming Yuan, Xue Jiao, Yufei Huang, Jing Li, Dong Li, Miaomiao Ji, Guoyun Wang
Summary: The study found that M1NVs can directly or indirectly inhibit the migration and invasion of EM-ESCs, as well as reduce tube formation. In a mouse model, M1NVs suppressed the development of endometriosis by reprogramming M2 macrophages, without causing organ damage.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Anna Maria Tolomeo, Ignazio Castagliuolo, Martina Piccoli, Michele Grassi, Fabio Magarotto, Giada De Lazzari, Ricardo Malvicini, Federico Caicci, Chiara Franzin, Melania Scarpa, Veronica Macchi, Raffaele De Caro, Imerio Angriman, Antonella Viola, Andrea Porzionato, Michela Pozzobon, Maurizio Muraca
Summary: The study compared the effects of MSCs and MSC-EVs in mice with experimental colitis, finding that EVs had a better therapeutic effect than MSCs, with a more predictable behavior, a safer therapeutic profile, and a higher therapeutic efficacy compared to their parent cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Victor Adrian Cortes-Morales, Luis Chavez-Sanchez, Leticia Rocha-Zavaleta, Sandra Espindola-Garibay, Alberto Monroy-Garcia, Marta Elena Castro-Manrreza, Guadalupe Rosario Fajardo-Orduna, Teresa Apresa-Garcia, Marcos Gutierrez-de la Barrera, Hector Mayani, Juan Jose Montesinos
Summary: This study found that MSCs derived from cervical cancer (CeCa-MSCs) have the ability to promote M2 macrophage polarization, unlike MSCs derived from normal cervix (NCx-MSCs). CeCa-MSCs increase the production of anti-inflammatory molecules (IL-10 and IL-1RA) to regulate antitumor immune responses.
Article
Biochemistry & Molecular Biology
Uliana Dyachkova, Maksim Vigovskiy, Nataliya Basalova, Anastasia Efimenko, Olga Grigorieva
Summary: Fibrosis is a significant contributor to mortality rate in developed countries, and multipotent mesenchymal stromal cells (MSC) can suppress its development. However, age-related chronic inflammation can induce MSC senescence and impair their antifibrotic properties. This study investigates the effects of alternatively activated macrophages (M2-type) and hydrogen peroxide (H2O2) on MSC senescence and fibroblast-to-myofibroblast differentiation. The results demonstrate that M2-macrophage-induced chronic inflammation and H2O2 exposure can induce MSC senescence and decrease their ability to inhibit fibroblast-to-myofibroblast differentiation. However, subsequent cultivation can reverse the senescence and restore the MSC's antifibrotic functions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Cansu Gorgun, Chiara Africano, Maria Chiara Ciferri, Nadia Bertola, Daniele Reverberi, Rodolfo Quarto, Silvia Ravera, Roberta Tasso
Summary: This study demonstrates that EVs derived from MSCs preconditioned with hypoxia inflammatory cytokines can attenuate inflammaging and alleviate age-related inflammation and mitochondrial dysfunction.
Article
Physiology
Changlin Zhang, Wei Liao, Weizhao Li, Mengxiong Li, Xiaoyu Xu, Haohui Sun, Yaohua Xue, Lixiang Liu, Jiehong Qiu, Chi Zhang, Xunzhi Zhang, Juntong Ye, Jingran Du, David Y. B. Deng, Wuguo Deng, Tian Li
Summary: This study found that treatment with hucMSC-EV significantly alleviates tubal inflammatory infertility caused by CT infection, and induces macrophage polarization via the NF-kappa B signaling pathway, improving the inflammatory microenvironment and inhibiting inflammation in the fallopian tubes.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Cell Biology
Neha Rana, Salwa Suliman, Niyaz Al-Sharabi, Kamal Mustafa
Summary: This study isolated and characterized extracellular vesicles (EVs) from different systems, and found that these EVs can modulate macrophage-mediated inflammation and healing responses. MSCp + BCP:EVs have higher immunomodulatory, angiogenic, and chemokine contents, leading to stronger modulation of macrophages.
Article
Biotechnology & Applied Microbiology
Kanglu Li, Guohua Yan, Hanji Huang, Mingjun Zheng, Ke Ma, Xiaofei Cui, Dejie Lu, Li Zheng, Bo Zhu, Jianwen Cheng, Jinmin Zhao
Summary: This study investigates the immunomodulatory and anti-inflammatory effects of human umbilical cord mesenchymal stem cells-derived extracellular vesicles (hUCMSCs-EVs) on osteoarthritis (OA). The results suggest that hUCMSCs-EVs promote the polarization of M2-type macrophages and the expression of IL-10, inhibit inflammation-associated factors in OA chondrocytes caused by IL-1 beta, and alleviate cartilage degradation during OA progression. The enriched miRNAs in hUCMSCs-EVs and the modulation of the PI3K-Akt signaling pathway may contribute to these therapeutic effects.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Junwen Han, Yuanyuan Shi, Gareth Willis, Jewel Imani, Min-Young Kwon, Gu Li, Ehab Ayaub, Sailaja Ghanta, Julie Ng, Narae Hwang, Konstantin Tsoyi, Souheil El-Chemaly, Stella Kourembanas, S. Alex Mitsialis, Ivan O. Rosas, Xiaoli Liu, Mark A. Perrella
Summary: The study highlights the importance of SDC2 in the cellular and paracrine function of human MSCs during sepsis. Silencing SDC2 in MSCs resulted in decreased bacterial clearance, increased tissue injury and mortality, as well as impaired resolution of inflammation. MSCs with silenced SDC2 also showed reduced ability to promote phagocytosis of apoptotic neutrophils and convert macrophages into a proresolution phenotype.
Article
Cell Biology
Mariana A. Antunes, Cassia L. Braga, Taina B. Oliveira, Jamil Z. Kitoko, Ligia L. Castro, Debora G. Xisto, Mariana S. Coelho, Nazareth Rocha, Rodrigo P. Silva-Aguiar, Celso Caruso-Neves, Eduarda G. Martins, Clara Fernandes Carvalho, Antonio Galina, Daniel J. Weiss, Jose R. Lapa e Silva, Miqueias Lopes-Pacheco, Fernanda F. Cruz, Patricia R. M. Rocco
Summary: The study compared MSCs from healthy and emphysematous donors, and observed their therapeutic effects in a mouse model of severe emphysema. MSCs from emphysematous donors exhibited gene downregulation and mitochondrial abnormalities, leading to inferior therapeutic outcomes compared to MSCs from healthy donors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Jessica Altemus, Neda Dadgar, Yan Li, Amy L. Lightner
Summary: The breakdown of gastrointestinal tract immune homeostasis leads to Crohn's disease (CD). Mesenchymal stem cells (MSCs) have demonstrated clinical efficacy in treating CD. This study investigated the interaction between adipose-derived MSCs and macrophages, and found that MSCs and their derived extracellular vesicles (EVs) can regulate macrophage polarization and promote the differentiation of monocytes into pro-inflammatory M1 phenotype in CD patients. Conversely, MSCs and their derived EVs from healthy individuals can drive monocytes to an anti-inflammatory M2 subset, suggesting the potential therapeutic use of MSCs and EVs in CD treatment.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Chih-Hsing Hung, Hua-Yu Hsu, Hsin-Ying Clair Chiou, Mei-Lan Tsai, Huey-Ling You, Yu-Chih Lin, Wei-Ting Liao, Yi-Ching Lin
Summary: Arsenic can promote macrophage polarization to M2 type, resulting in the epithelial-mesenchymal transition of lung epithelial cells. This study found that arsenic induces repolarization of M1 macrophages to M2 macrophages through the autophagy/mitophagy pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Fabiany da Costa Goncalves, Sander S. Korevaar, Maitane Ortiz Virumbrales, Carla C. Baan, Marlies E. J. Reinders, Ana Merino, Eleuterio Lombardo, Martin J. Hoogduijn
Summary: Mesenchymal stromal cells (MSC) show promise as a therapy for inflammatory diseases, but their large size leads to entrapment in the lungs after intravenous infusion, limiting their effectiveness. Researchers have developed nanometer-sized particles from MSC membranes (membrane particles, MP) to extend the reach of MSC immunoregulatory therapy beyond the lungs. These particles exhibit immunomodulatory properties and are taken up by macrophages and human umbilical vein endothelial cells (HUVEC) in a dose, time, and temperature-dependent manner. The uptake of MP depends on specific endocytic pathways and is influenced by cell type and inflammatory conditions, with anti-inflammatory M2 macrophages showing increased uptake compared to pro-inflammatory M1 macrophages.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell & Tissue Engineering
Ricardo Malvicini, Diego Santa-Cruz, Giada De Lazzari, Anna Maria Tolomeo, Cecilia Sanmartin, Maurizio Muraca, Gustavo Yannarelli, Natalia Pacienza
Summary: This study standardized a quick, cheap, and reproducible in vitro macrophage assay to evaluate and estimate the anti-inflammatory activity of MSC-sEVs.
