Article
Engineering, Biomedical
Renao Yan, Qiming He, Yiqing Liu, Peng Ye, Lianghui Zhu, Shanshan Shi, Jizhou Gou, Yonghong He, Tian Guan, Guangde Zhou
Summary: Fibrosis is an essential stage in the progression of chronic liver disease and plays a crucial role in assessing its severity. While conventional staining methods can provide structural information, the expensive costs and inconsistent processes have prompted the development of a virtual staining solution using prior-guided generative adversarial networks. This study demonstrates the effectiveness of generating Masson trichrome stained images from H&E stained ones, achieving superior performance compared to previous approaches.
COMPUTERIZED MEDICAL IMAGING AND GRAPHICS
(2023)
Article
Chemistry, Analytical
Jin-Mei Li, Ke-Rang Wang
Summary: The study explored the versatility of multicolor imaging of human tissues using staining with perylene monoimide-mannose conjugates PMI-Man and co-staining with PMI-Man and eosin. The results showed perfect histological morphology in confocal fluorescence microscopy and light microscopy, providing a universal colorful staining method for both cancer tissues and normal tissues.
Article
Multidisciplinary Sciences
Anca Remes, Marie Noormalal, Nesrin Schmiedel, Norbert Frey, Derk Frank, Oliver J. Mueller, Markus Graf
Summary: Pathological remodeling of the extracellular matrix is a key feature of cardiovascular disease. Accurately quantifying fibrosis deposition in histological sections is essential for research, but currently available automatic tools are limited by their performance under different conditions and lack of flexibility in evaluating staining methods. Our novel machine learning-based tool, FibroSoft, demonstrated its feasibility in assessing fibrosis in a mouse model of cardiac hypertrophy and heart failure. The results were consistent and correlated well with Western blot analysis. This tool can also be applied to different staining methods, making it suitable for various histology segmentation and quantification studies.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Analytical
Sakiko Akaji, Tomoya Sagawa, Akiko Honda, Natsuko Miyasaka, Kaori Sadakane, Takamichi Ichinose, Hirohisa Takano
Summary: This study developed a combined method for morphological observation and Raman spectral acquisition on the identical tissue slide by employing a decolorization step to remove fluorescence, enabling simultaneous identification of biological responses and materials in tissues.
Article
Multidisciplinary Sciences
Mirae Lee, Jiwon Woo, Doh-Hee Kim, Yu-Mi Yang, Eunice Yoojin Lee, Jung-Hee Kim, Seok-Gu Kang, Jin-Kyung Shim, Jeong-Yoon Park
Summary: The team proposed an optimized MAP-based method for paper-thin samples and explored its application in tissue clearing and three-dimensional imaging. Through rapid clearing and imaging, this method can be used to study the vasculature and neuronal networks of various tissues in healthy and diseased environments.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Hiroyuki Sasaki, Hisako Arai, Emi Kikuchi, Hideki Saito, Keiko Seki, Takeshi Matsui
Summary: The use of Mayer's hematoxylin followed by Reynold's lead citrate solution is suggested as a reliable and promising alternative to Uranyl acetate (UA) staining for ultrathin sections in biological electron microscopy.
SCIENTIFIC REPORTS
(2022)
Article
Automation & Control Systems
Nand Kishor Yadav, Mukesh Saraswat
Summary: This paper introduces a new method for RGB-Depth image segmentation, which achieves optimized fuzzy clustering and segmentation through a random Henry gas solubility optimization algorithm and superpixel aggregation. Evaluating its performance on a standard dataset, the proposed method outperforms traditional methods.
ENGINEERING APPLICATIONS OF ARTIFICIAL INTELLIGENCE
(2022)
Article
Plant Sciences
Fei Nan, Yang Song, Xun Yu, Chenwei Nie, Yadong Liu, Yali Bai, Dongxiao Zou, Chao Wang, Dameng Yin, Wude Yang, Xiuliang Jin
Summary: This study presents a novel method for maize leaf disease classification using the post-segmentation image data from a multi-sensor synchronized RGB-D camera. Pre-segmentation models performed slightly better, but post-segmentation models provided more practical results with shorter prediction times. Among the post-segmentation models, Resnet50 and MobilenetV2 models showed the best accuracy.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Cell Biology
Kayvan Forouhesh Tehrani, Emily G. Pendleton, W. Michael Southern, Jarrod A. Call, Luke J. Mortensen
Summary: Imaging-based metrics for periodic structures of muscular and osseous tissues were analyzed using Fourier transform filters to extract angular orientation and periodicity, providing insights into tissue health and regeneration. Results from assessing mouse skeletal muscles and bone collagen structure indicated the potential of quantifying musculoskeletal tissues during development and regeneration.
CONNECTIVE TISSUE RESEARCH
(2021)
Article
Computer Science, Information Systems
Ahmet Ozcan, Omer Cetin
Summary: This study introduces a simple and effective method to fuse RGB-D and thermal sensor data for more accurate human detection. By physically fixing the sensors and extracting/matching feature points using computer vision, the proposed method can be used in real-time applications and improves human detection accuracy.
Article
Ophthalmology
Lei-Chi Wang, Wen-Jung Lo, Yu-Bai Chou, Shih-Jen Chen, Tai-Chi Lin, Teh-Ying Chou
Summary: We propose a new tissue submission procedure for assessing retinal tissue histology and immunohistochemical features without additional equipment or storage facilities. The adequacy ratio of specimens submitted using the new method was comparable to that of the flat-mount method, producing high-quality images and enabling immunohistochemical analysis. Our study provides additional histological and immunohistochemical evidence of cellular components in retinal tissues based on a large number of human tissue samples.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Rong Yang, Jianfeng Li, Yifan Wu, Xinli Jiang, Shuang Qu, Qiang Wang, Hongwei Liang, Ke Zen
Summary: Methylation modification of small RNAs is critical for their biogenesis and function. LC-MS/MS analysis of small RNA methylation is limited due to the difficulty of purifying specific small RNA. This study presents a novel method for efficiently purifying small RNA from human tissues for LC-MS/MS analysis.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Food Science & Technology
Wenzhe Gao, Zhiyu Zhou, Kai Wang, Xingshu Wang, Zikang Guo, Chao Li, Zhaohui Wang, Mei Shi
Summary: A new method was developed to determine the concentration and distribution of iron in wheat grain tissues. The study found significant variations in the concentration of iron among different wheat cultivars and locations. The research also identified the main contributors to iron content in wheat grains in different regions of China. Overall, this study provides valuable information for improving agronomic practices and wheat breeding for increasing iron biofortification.
JOURNAL OF CEREAL SCIENCE
(2023)
Article
Mathematics, Interdisciplinary Applications
Qin Shi, Huansheng Song, Shijie Sun
Summary: The proposed method utilizes depth map for extrinsic parameter calibration, by automatically detecting planes in the 3D point cloud. Experimental results demonstrate high accuracy and automation of the method for estimating extrinsic parameters of a camera.
DISCRETE DYNAMICS IN NATURE AND SOCIETY
(2021)
Article
Nanoscience & Nanotechnology
Yi-Ying Lu, Hsiao-Ching Yu, You-Xin Wang, Chih-Keng Hung, You-Ren Chen, Jie Jhou, Peter Tsung-Wen Yen, Jui-Hung Hsu, Raman Sankar
Summary: This study demonstrates a red, green, and blue (RGB) color contrast method for identifying the thickness of Indium selenide (InSe). By analyzing the optical contrasts from the RGB channels, the thickness of InSe can be quantitatively correlated. The effective refractive indices are extracted and the wavelength-dependent refractive indices are considered to improve the accuracy of thickness estimation. In addition, the complex refractive index spectra generated from ab initio numerical calculation results are used for thickness identification. This work has significant implications for the study and practical applications of layered materials.
Article
Endocrinology & Metabolism
Encarnacion Torres, Inmaculada Velasco, Delphine Franssen, Violeta Heras, Francisco Gaytan, Silvia Leon, Victor M. Navarro, Rafael Pineda, M. Luz Candenas, Antonio Romero-Ruiz, Manuel Tena-Sempere
Summary: The study explored the role of NK2R in the neuroendocrine control of the reproductive axis using a novel Tacr2 knockout mouse model. Results showed that NK2R stimulation elicited LH responses in mice, with partial suppression of basal and stimulated LH secretion in Tacr2 knockout mice, impacting LH pulsatility and breeding intervals. NK2R plays a modest role in the gonadotropic axis, overlapping with other tachykinin receptors.
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2021)
Article
Plant Sciences
Beatriz Carmona-Hidalgo, Isabel Gonzalez-Mariscal, Adela Garcia-Martin, Maria E. Prados, Francisco Ruiz-Pino, Giovanni Appendino, Manuel Tena-Sempere, Eduardo Munoz
Summary: Delta(9)-THCA shows antifibrotic and antiinflammatory activities in vitro and in vivo, suggesting its potential therapeutic role in liver fibrosis and NAFLD management.
Article
Endocrinology & Metabolism
D. Franssen, A. Barroso, F. Ruiz-Pino, M. J. Vazquez, D. Garcia-Galiano, J. M. Castellano, R. Onieva, M. Ruiz-Cruz, M. Poutanen, F. Gaytan, C. Dieguez, L. Pinilla, M. Lopez, J. Roa, M. Tena-Sempere
Summary: Reproduction is closely linked to energy and metabolic status, with AMPK signaling in GnRH neurons playing a crucial role in metabolic control of the female reproductive axis, particularly during puberty and negative energy balance.
METABOLISM-CLINICAL AND EXPERIMENTAL
(2021)
Review
Endocrinology & Metabolism
Leandro Soriano-Guillen, Manuel Tena-Sempere, Carlos E. Seraphim, Ana C. Latronico, Jesus Argente
Summary: Puberty is a crucial biological process involving sexual and somatic maturation, regulated by genetic, endogenous, and environmental factors. Recent research has revealed the essential roles of kisspeptins and neurokinin B in modulating the signaling pathways of pubertal maturation, as well as the effects of melanocortins and metabolic sensors. Precocious puberty, primarily affecting females, can be caused by alterations in the central nervous system and genetic mutations, such as those in the MKRN3 gene. Understanding the genotype-phenotype relationship in patients with precocious puberty is important for further research.
JOURNAL OF NEUROENDOCRINOLOGY
(2022)
Article
Oncology
Alvaro Arjona-Sanchez, Ana Martinez-Lopez, Francisca Valenzuela-Molina, Blanca Rufian-Andujar, Sebastian Rufian-Pena, Angela Casado-Adam, Juan Manuel Sanchez-Hidalgo, Lidia Rodriguez-Ortiz, Francisco Javier Medina-Fernandez, Cesar Diaz-Lopez, Melissa Granados-Rodriguez, Rosa Ortega-Salas, Justo P. Castano, Manuel Tena-Sempere, Javier Briceno-Delgado, Antonio Romero-Ruiz
Summary: By analyzing the Ki-67 proliferation index, the HG-PMP category according to the PSOGI classification was divided into two subcategories, showing significant differences in overall survival (OS) and disease-free survival (DFS) for patients, indicating high prognostic value.
ANNALS OF SURGICAL ONCOLOGY
(2022)
Article
Obstetrics & Gynecology
Miguel Angel Sanchez-Garrido, David Garcia-Galiano, Manuel Tena-Sempere
Summary: Nutritional and hormonal fluctuations during the periconceptional, prenatal, and early postnatal periods have been shown to impact pubertal maturation and adult gonadotropic function in both sexes. Exposure to environmental chemicals and maternal stress can also negatively influence reproductive function. Research on the underlying neuroendocrine pathways may provide insights into reproductive disorders.
HUMAN REPRODUCTION UPDATE
(2022)
Review
Endocrinology & Metabolism
Veronica Sobrino, Maria Soledad Avendano, Cecilia Perdices-Lopez, Manuel Jimenez-Puyer, Manuel Tena-Sempere
Summary: In late 2003, the identification of the reproductive roles of kisspeptins and Gpr54 led to a surge in research interest and established fundamental concepts in physiological roles of kisspeptins. This early knowledge set the stage for more recent in-depth studies on neuronal networks involving Kiss1 neurons and their regulation mechanisms.
FRONTIERS IN NEUROENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Amparo Romero-Pico, Marta G. Novelle, Omar Al-Massadi, Daniel Beiroa, Marta Tojo, Violeta Heras, Francisco Ruiz-Pino, Ana Senra, Miguel Lopez, Clemence Blouet, Manuel Tena-Sempere, Ruben Nogueiras, Carlos Dieguez
Summary: Weight gain is a common symptom of decreased estradiol levels due to menopause or surgical ovariectomy. This study shows that the kappa-opioid receptor system plays a role in mediating weight gain associated with estradiol withdrawal. It also suggests that the hypothalamic mTOR pathway is involved in regulating weight and adiposity in ovariectomized mice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Endocrinology & Metabolism
Cecilia Perdices-Lopez, Maria S. Avendano, Alexia Barroso, Francisco Gaytan, Francisco Ruiz-Pino, Maria J. Vazquez, Silvia Leon, Yong Bhum Song, Veronica Sobrino, Violeta Heras, Antonio Romero-Ruiz, Juan Roa, Federico Mayor Jr, Cristina Murga, Leonor Pinilla, Ursula B. Kaiser, Manuel Tena-Sempere
Summary: The study reveals the significant role of G protein-coupled receptor kinase-2 (GRK2) in regulating the timing of puberty. GRK2 negatively regulates kisspeptin actions in GnRH neurons, thus influencing the onset of puberty, particularly under conditions of nutritional deficiency. Pharmacological studies and conditional ablation experiments provide evidence for the expression and regulatory role of GRK2 in GnRH neurons.
METABOLISM-CLINICAL AND EXPERIMENTAL
(2022)
Review
Endocrinology & Metabolism
Jesus Argente, Leo Dunkel, Ursula B. Kaiser, Ana C. Latronico, Alejandro Lomniczi, Leandro Soriano-Guillen, Manuel Tena-Sempere
Summary: Puberty, a major maturational event, is determined by genetic factors and influenced by endogenous and environmental cues. Research progress has been made in understanding the neuroendocrine networks governing puberty, but the precise timing and mechanisms of puberty and its alterations are still being deciphered. This review provides a comprehensive overview of the physiological basis of puberty, including the roles of kisspeptins and other central transmitters, the underlying molecular and endocrine mechanisms, and the pathways involved in pubertal modulation. Molecular features of precocious and delayed puberty are also summarized. The review highlights the need for further research in this active field of endocrinology.
LANCET DIABETES & ENDOCRINOLOGY
(2023)
Editorial Material
Endocrinology & Metabolism
Juan M. Castellano, Ana B. Ariza-Jimenez, Manuel Tena-Sempere
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Editorial Material
Endocrinology & Metabolism
Jesus Argente, Manuel Tena-Sempere
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Review
Endocrinology & Metabolism
Vincent Prevot, Manuel Tena-Sempere, Nelly Pitteloud
Summary: Pulsatile secretion of GnRH is essential for the hypothalamic-pituitary-gonadal axis and is involved in brain maturation, odor discrimination, and cognition. This review highlights the importance of the first postnatal activation of GnRH, known as minipuberty, for reproductive and nonreproductive functions. Additionally, it discusses the potential therapeutic effects of pulsatile GnRH therapy in neurodevelopmental cognitive disorders and pathological aging.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Review
Endocrinology & Metabolism
Greg M. Anderson, Jennifer W. Hill, Ursula B. Kaiser, Victor M. Navarro, Ken K. Ong, John R. B. Perry, Vincent Prevot, Manuel Tena-Sempere, Carol F. Elias
Summary: An individual's nutritional status has a significant impact on sexual maturation, with chronic energy insufficiency delaying puberty onset and energy abundance advancing it. The timing of puberty has profound consequences for human health, with late puberty increasing the risks of various disorders and early puberty associated with adult obesity, diabetes, cardiovascular diseases, and certain cancers. Recent advances in the field have been achieved through the development of molecular tools and population genetics, leading to a better understanding of the nutritional and metabolic control of pubertal development.
NATURE REVIEWS ENDOCRINOLOGY
(2023)
Article
Endocrinology & Metabolism
Edward Milbank, Nathalia R. V. Dragano, Ismael Gonzalez-Garcia, Marcos Rios Garcia, Veronica Rivas-Limeres, Liliana Perdomo, Gregory Hilairet, Francisco Ruiz-Pino, Patricia Mallegol, Donald A. Morgan, Ramon Iglesias-Rey, Cristina Contreras, Luisa Vergori, Juan Cunarro, Begona Porteiro, Aleix Gavalda-Navarro, Rebecca Oelkrug, Anxo Vidal, Juan Roa, Tomas Sobrino, Francesc Villarroya, Carlos Dieguez, Ruben Nogueiras, Cristina Garcia-Caceres, Manuel Tena-Sempere, Jens Mittag, M. Carmen Martinez, Kamal Rahmouni, Ramaroson Andriantsitohaina, Miguel Lopez
Summary: Milbank et al. demonstrate that targeted delivery of AMPK alpha 1 in SF1 neurons of the VMH using small extracellular vesicles can lead to weight loss through increased brown adipose tissue thermogenesis, independent of feeding.