Article
Biochemistry & Molecular Biology
Ning Wang, Haiying Zhang, Xiulin Cui, Chao Ma, Linghui Wang, Wenguang Liu
Summary: Runx3 re-expression in B16-F10 melanoma cells alters their shape, attenuates migration, and suppresses pulmonary metastasis, revealing a potential therapeutic strategy for metastatic melanoma. Microarray analysis uncovers an altered transcriptional profile rich in annotations related to adhesion, actin cytoskeleton, and extracellular matrix proteins, with prognostic value demonstrated by MAL and VILL genes. Knockdown of MAL gene expression in B16-F0 cells enhances migratory and invasive traits, highlighting the significance of adhesion and actin cytoskeleton in melanoma metastasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Kimberly N. Kremer, Alan Buser, Dean Thumkeo, Shuh Narumiya, Jordan Jacobelli, Roberta Pelanda, Raul M. Torres
Summary: Cancer and chronic infections increase levels of lysophosphatidic acid (LPA), which impairs T cell function. Targeting LPA signaling can rescue T cell function and promote antiviral and antitumor immunity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Medicine, Research & Experimental
Lorena Rodriguez, Gabriela Di Venosa, Martin A. Rivas, Angeles Juarranz, Francisco Sanz-Rodriguez, Adriana Casas
Summary: The study found that Ras transfection affects cell morphology, extracellular matrix, and cell-cell adhesion, resulting in increased resistance to photodynamic therapy (PDT).
Article
Biology
Rafael Moreno-Gomez-Toledano, Maria Arenas, Esperanza Velez-Velez, Ricardo J. Bosch
Summary: The xenoestrogen Bisphenol A(BPA) has implications for the endocrine systems and kidneys. Recent renal studies have shown that BPA can induce alterations in the cytoskeleton and cell adhesion mechanisms, contributing to the progression of renal diseases. The presence of BPA in the urine of almost the entire population emphasizes the need to reevaluate its supposed safe exposures.
Article
Biotechnology & Applied Microbiology
Yang Qiu, Chuntao Lei, Jieyu Zeng, Yaru Xie, Yiling Cao, Qian Yuan, Hua Su, Zhentao Zhang, Chun Zhang
Summary: Focal segmental glomerulosclerosis (FSGS) is the most common glomerular disorder causing end-stage renal diseases worldwide. Our study found that increased expression of lysosomal protease AEP protected podocytes from injury by regulating the homeostasis of the cytoskeleton.
Article
Chemistry, Multidisciplinary
Jennifer Soto, Yang Song, Yifan Wu, Binru Chen, Hyungju Park, Navied Akhtar, Peng-Yuan Wang, Tyler Hoffman, Chau Ly, Junren Sia, SzeYue Wong, Douglas O. Kelkhoff, Julia Chu, Mu-Ming Poo, Timothy L. Downing, Amy C. Rowat, Song Li
Summary: The role of cytoskeletal structures and mechanical properties in cell fate determination and reprogramming efficiency has been investigated. It is found that reducing cellular tension or adhesion can modulate epigenetic marks and increase accessibility for gene activation, thus enhancing induced neuronal (iN) reprogramming. Furthermore, micro- and nano-topographic surfaces that reduce cell adhesion can enhance iN reprogramming. These findings suggest that the actin cytoskeleton and focal adhesions play a crucial role in epigenetic regulation for cell fate determination.
Article
Multidisciplinary Sciences
A. Hackl, E. Nuesken, J. Voggel, S. E. D. Abo Zed, J. Binz-Lotter, D. Unnersjoe-Jess, C. Mueller, G. Fink, K. Bohl, E. Wiesner, P. Diefenhardt, C. Dafinger, H. Chen, M. Wohlfarth, R. -u. Mueller, M. J. Hackl, B. Schermer, K. -d. Nuesken, L. T. Weber
Summary: The study found that mycophenolate mofetil can ameliorate podocyte damage by restoring the Ca2+-actin cytoskeleton axis, thereby improving proteinuria and nephritis. These findings are of great importance for enhancing the therapeutic effect of proteinuric glomerulopathies.
SCIENTIFIC REPORTS
(2023)
Article
Engineering, Environmental
Xiyan Mu, Jia Liu, Hui Wang, Lilai Yuan, Chengju Wang, Yingren Li, Jing Qiu
Summary: The study found that exposure to BPF inhibited the cognitive ability of zebrafish, while increasing the number of microglia and decreasing the number of neurons in the brain. In addition, BPF exposure triggered significant inflammatory response and enhanced phagocytic activity, in which microglia played a dominant role. Furthermore, BPF also affected specific neurological diseases such as movement disorders and neuromuscular diseases, but with different involved genes.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Oncology
Paul Munson, Juraj Adamik, Felix J. Hartmann, Patricia M. B. Favaro, Daniel Ho, Sean C. Bendall, Alexis J. Combes, Matthew F. Krummel, Karen Zhang, Robin K. Kelley, Lisa H. Butterfield
Summary: Hepatocellular cancer tumors express a-Fetoprotein (AFP), which inhibits dendritic cell differentiation and maturation, and blocks oxidative phosphorylation. Tumor-derived AFP increases glycolytic capacity and glucose dependence of dendritic cells, leading to increased glucose uptake and lactate secretion. Furthermore, AFP bound with polyunsaturated fatty acids (PUFAs) skews dendritic cell metabolism and promotes immune suppression.
Article
Cell Biology
Alicia M. Salvi, Jennifer L. Bays, Samantha R. Mackin, Rene-Marc Mege, Kris A. DeMali
Summary: This research demonstrates that force transmission can increase glucose uptake by stimulating GLUT1, and this process requires non-muscle myosin IIA and ankyrin G. Ankyrin G acts as a bridge between force-transducing receptors and GLUT1, crucial for cells to regulate glucose uptake under tension.
NATURE CELL BIOLOGY
(2021)
Review
Pediatrics
Sanja Sever
Summary: The selectivity of the glomerular filter is established by the interaction among its core components, including glomerular endothelial cells, the glomerular basement membrane, and podocytes, with functional impairment of any leading to proteinuria. Podocyte injury is associated with various forms of glomerular diseases in both human and experimental models, such as minimal change disease, focal segmental glomerulosclerosis, and diabetes mellitus. Modifying the actin cytoskeleton in podocytes pharmacologically holds promise as a novel therapeutic approach for podocyte-dependent chronic kidney diseases.
PEDIATRIC NEPHROLOGY
(2021)
Article
Multidisciplinary Sciences
Ka Man Carmen Chan, Ashley L. Arthur, Johannes Morstein, Meiyan Jin, Abrar Bhat, Dorte Schlesinger, Sungmin Son, Donte A. Stevens, David G. Drubin, Daniel A. Fletcher
Summary: Fusion-associated small transmembrane (FAST) proteins are a diverse family of nonstructural viral proteins that drive cell-cell fusion, increasing viral spread and pathogenicity. Unlike viral fusogens with tall ectodomains, FAST proteins have short fusogenic ectodomains that cannot bridge the intermembrane gap between neighboring cells. This work suggests that localized mechanical pressure on the plasma membrane coupled to a membrane-disruptive ectodomain is sufficient to drive cell-cell fusion.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Immunology
Gustavo P. de Almeida, Peter Lichtner, Gertrud Eckstein, Tonio Brinkschmidt, Chang-Feng Chu, Shan Sun, Julian Reinhard, Sophia C. Madler, Markus Kloeppel, Mareike Verbeek, Christina E. Zielinski
Summary: Tissue-resident memory T cells (T-RM) play crucial roles in host defense in various tissues and barrier sites. However, the maintenance and regulatory checkpoints of human T-RM cells are still poorly understood. A study on patients receiving allogeneic hematopoietic stem cell transplantation (allo-HSCT) revealed long-term sequestration of host T cells in human skin. This finding sheds light on the characteristics and functions of skin-resident T-RM cells in humans.
SCIENCE IMMUNOLOGY
(2022)
Article
Environmental Sciences
Michal Stefancik, Lucie Valkova, Jana Veverkova, Jan Balvan, Tomas Vicar, Petr Babula, Josef Masek, Pavel Kulich, Monika Pavkova Goldbergova
Summary: The study focused on the impact of TiO2 and Ni nanoparticles on human osteoblasts, finding that both types of nanoparticles cause changes in cell size, actin expression, and gene expression in physiological and osteoarthritic osteoblasts.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Environmental Sciences
Nadia Oliveira, Helena Marcelino, Regina Azevedo, Ignacio Verde
Summary: This study found that BPA is a concentration-dependent vasodilator of human umbilical arteries, and through gene expression analysis, it was found that BPA can influence the expression of various proteins, including ion channels and proteins involved in the cGMP pathway. These findings contribute to a better understanding of the changes induced by BPA.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Editorial Material
Urology & Nephrology
Jordi Bover, Ricardo Bosch, Jose Luis Gorriz, Pablo Urena, Alberto Ortiz, Iara daSilva, Ramon A. Garcia-Trabanino, Miguel Hueso, Pedro Trinidad, Aquiles Jara, Monica Furlano, Rosana Gelpi, Ana Vila-Santandreu, Cesar A. Restrepo, Maya Sanchez-Baya, Carolt Arana, Marian Goicoechea, Veronica Coll, Julian Segura, Orlando Gutierrez, Kamyar Kalantar-Zadeh, Emilio Sanchez, Alejandro Ferreiros, Rafael Garcia-Maset
Letter
Cardiac & Cardiovascular Systems
Sandra Sanchez-Esteban, Alberto Cook, Paula Reventun, Carlos Zaragoza, JoseLuis Zamorano, Marta Saura
REVISTA ESPANOLA DE CARDIOLOGIA
(2022)
Article
Biochemistry & Molecular Biology
Rafael Moreno-Gomez-Toledano, Maria Arenas, Carmen Munoz-Moreno, Nuria Olea-Herrero, Paula Reventun, Adriana Izquierdo-Lahuerta, Alba Anton-Cornejo, Marta Gonzalez-Santander, Carlos Zaragoza, Marta Saura, Ricardo J. Bosch
Summary: Research has shown that BPA exposure can lead to kidney alterations that resemble early diabetic nephropathy, such as increased kidney damage biomarkers, urinary albumin excretion, and hypertension. Additionally, male mice exposed to BPA exhibited more severe adverse effects, displaying a pattern of sexual dimorphism.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Article
Endocrinology & Metabolism
Rafael Moreno-Gomez-Toledano, Esperanza Velez-Velez, Maria Arenas, Marta Saura, Ricardo J. Bosch
Summary: This study investigated the relationship between urinary BPS and BPF and diabetes, and found a significant relationship between urinary BPS, but not BPF, and diabetes risk. Additionally, there was a relationship between both compounds and hypertension, and a slight relationship between BPF and dyslipidemia. This indicates that BPA substitute molecules do not exempt the population from potential health risks.
WORLD JOURNAL OF DIABETES
(2022)
Article
Environmental Sciences
Rafael Moreno-Gomez-Toledano
Summary: Plastic waste pollution is a major environmental issue, and the substitute molecules like bisphenol F or S may pose similar health risks as bisphenol A, significantly impacting renal and cardiovascular diseases.
ENVIRONMENTAL POLLUTION
(2022)
Article
Biochemistry & Molecular Biology
Sandra Sanchez-Esteban, Mercedes Castro-Pinto, Alberto Cook-Calvete, Paula Reventun, Maria Delgado-Marin, Lucia Benito-Manzanaro, Ignacio Hernandez, Jose Lopez-Menendez, Jose Luis Zamorano, Carlos Zaragoza, Marta Saura
Summary: Calcific aortic valve disease (CAVD) is a common age-related disease. This study found that the expression of integrin-linked kinase (ILK) is downregulated in CAVD patients' aortic valves and is negatively correlated with valve calcification markers. Silencing ILK expression in valve endothelial cells promoted endothelial-to-mesenchymal transition (EndMT) and osteoblastic phenotype switch. Nitric oxide (NO) treatment prevented VEC transdifferentiation and calcification, suggesting the involvement of the ILK-eNOS signaling pathway in valve calcification.
Letter
Hematology
Pablo Toledano-Sanz, Paula Reventun, Maria Viskadourou, William O. Osburn, Nunzio Alcharani, Charles J. Lowenstein, Marios Arvanitis
Article
Emergency Medicine
Eva Garcia-Carpintero Blas, Cristina Gomez-Moreno, Rafael Moreno-Gomez-Toledano, Helena Ayuso-del-Olmo, Eva Rodrigo-Guijarro, Silvia Polo-Martinez, Cesar Manso Perea, Esperanza Velez-Velez
Summary: This study describes the experiences of nursing staff in the emergency department in caring for patients with mental health problems, as well as their experiences in healthcare settings. The main findings of the study highlight the need to train emergency nurses in caring for individuals with mental health issues, including bias education, and the implementation of standardized protocols. While emergency nurses never doubted their ability to care for individuals with mental health disorders, they recognized the need for support from specialized professionals at critical moments.
JOURNAL OF EMERGENCY NURSING
(2023)
Article
Multidisciplinary Sciences
Emma Bouck, Marios Arvanitis, William K. Osburn, Yaqiu L. Sang, Paula J. Reventun, Homa S. Ahmadzia, Nicholas P. Smith, Charles P. Lowenstein, Alisa P. Wolberg
Summary: Oral contraceptive (OC) use increases the risk of venous thromboembolism. This study investigates the effect of OC hormones on endothelial cell (EC) procoagulant activity and the potential interaction between OC hormones and estrogen receptors and inflammation. The results show that the OC hormones ethinyl estradiol and drospirenone do not directly enhance the thrombin generation potential of primary ECs in vitro.
Article
Biochemistry & Molecular Biology
Rafael Moreno-Gomez-Toledano, Maria Delgado-Marin, Sandra Sanchez-Esteban, Alberto Cook-Calvete, Sara Ortiz, Ricardo J. Bosch, Marta Saura
Summary: Plastic production, disposal, and recycling systems pose significant challenges to the planet's health by releasing endocrine disruptors into the environment. The study focused on the mixture of bisphenols (BPmix) in a large human cohort and found a positive association between BPmix and heart disease. Further experiments demonstrated that BPmix had a higher effect on cell viability compared to BPA, and its combination induced apoptosis in endothelial cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cardiac & Cardiovascular Systems
P. Reventun, S. Sanchez-Esteban, A. Cook-Calvete, M. Delgado-Marin, C. Roza, S. Jorquera-Ortega, I. Hernandez, L. Tesoro, L. Botana, J. L. Zamorano, C. Zaragoza, M. Saura
Summary: Endothelial dysfunction is an early event in coronary microvascular disease, and endothelial ILK plays a crucial role in maintaining coronary microvascular function and cardiac performance. Deletion of endothelial ILK impairs cardiac function, induces adverse cardiac remodeling, and leads to ischaemic-like events and microvascular disease.
BASIC RESEARCH IN CARDIOLOGY
(2023)
Article
Genetics & Heredity
Michele Di Nunzio, Carme Barrot-Feixat, David Gangitano
Summary: This study developed five SNaPshotTM assays for nine chloroplast markers to differentiate between marijuana crop type and hemp crop type. The testing of 122 cannabis samples successfully distinguished between marijuana and hemp, and genetic differences between different groups based on biogeographical origin and crop type were observed. Phylogenetic analysis and principal component analysis provided valuable information about the genetic relationship and differentiation between different cannabis samples.
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2024)
Article
Medicine, Legal
M. Di Nunzio, A. Della Valle, A. Serino, F. Corrado, C. Di Nunzio
Summary: The study presents a case of a 61-year-old man who was found dead in an agricultural plot, revealing a dog pack attack. By utilizing a multi-disciplinary approach, including crime scene analysis and forensic genetics DNA correlations, the researchers were able to identify the active participants based on dental abnormalities.
FORENSIC SCIENCE MEDICINE AND PATHOLOGY
(2023)