Article
Immunology
Hongfei Tian, Jing Xing, Xiaoqian Tang, Heng Chi, Xiuzhen Sheng, Wenbin Zhan
Summary: The T-bet transcription factor in flounder plays a key role in immune responses, with abundant expression in specific organs and significant upregulation after infection. T-bet(+) cells were identified in lymphocytes and B cells, and found to proliferate in response to specific cytokines. These results suggest that T-bet may act as a master regulator of Th1-type immune responses in flounder CD4(+) T lymphocytes.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jae Wook Jung, Ae Rin Lee, Jaesung Kim, Young Rim Kim, Jassy Mary S. Lazarte, Jung Suk Lee, Kim D. Thompson, Hyeongsu Kim, Tae Sung Jung
Summary: The study successfully generated monoclonal antibodies against CD8 T lymphocytes in olive flounder and confirmed their specificity. The populations of CD4 and CD8 T lymphocytes significantly increased in fish infected or immunized, with up-regulation of Th1-related genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Yanbo Hao, Xiaoqian Tang, Jing Xing, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Summary: The Fc receptor plays a critical role in mediating the phagocytosis and bactericidal activity of IgM(+) B lymphocytes in flounder. It increases phagocytosis rates and affects intracellular reactive oxygen species levels. Additionally, blocking Fc receptor or Fc fragment of IgM reduces phagocytosis rates in mIgM(+) B lymphocytes.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Fisheries
Mengmeng Zhao, Jing Xing, Xiaoqian Tang, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Summary: By cloning interleukin-2 receptor subunit gamma (IL-2R gamma) in flounder and producing specific antibodies, this study characterized the expression of flounder IL-2R gamma (fIL-2R gamma) at transcriptional and cellular levels and investigated its interaction with flounder IL-2 (fIL-2). The results demonstrated that fIL-2R gamma was expressed on both T and B lymphocytes and the binding of fIL-2R gamma and fIL-2 activated CD4 T lymphocytes.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Genetics & Heredity
Fan Yang, Saisai Liu, Jiangbo Qu, Quanqi Zhang
Summary: The Pomstna gene in Japanese flounder has been identified as a negative regulator of muscle growth and differentiation, and it operates through a different regulatory mechanism than Pomstnb. This study suggests that muscle proliferation and differentiation are not only regulated through the TGF-beta signaling pathway, but also through other unknown mechanisms.
Article
Biochemistry & Molecular Biology
Chang Shu, Lijuan Wang, Congcong Zou, Xungang Tan, Yuxia Zou, Lingming Kong, Zhihao Wu, Qiaowan Wu, Ling Wang, Guoyu Wang, Ze Li, Feng You
Summary: This study successfully obtained bioactive recombinant Foxl2 and Dmrt1 proteins and demonstrated that recombinant Dmrt1 can alter the sex phenotype in fish. Furthermore, the injection of recombinant Foxl2 and Dmrt1 resulted in changes in the transcription levels of cyp19a and its transcription factors. These findings reveal the important regulatory roles of Foxl2 and Dmrt1 in fish gonadal differentiation and provide a new approach for sex control in fish aquaculture.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Immunology
Xiaoqian Tang, Xinbiao Ma, Jing Cao, Xiuzhen Sheng, Jing Xing, Heng Chi, Wenbin Zhan
Summary: This study found that Hirame novirhabdovirus (HIRRV) can invade mIgM(+) B lymphocytes and elicit differential immune response at different temperatures.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Fisheries
Yanbo Hao, Xiaoqian Tang, Jing Xing, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Summary: The study reveals that phosphorylated Syk plays a key role in FcR-mediated phagocytosis and bactericidal activity of mIgM+ B lymphocytes, affecting intracellular reactive oxygen species levels and the expression of related genes.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Fisheries
Kun Yang, Xueshu Zhang, Zengyu Liu, Weiqun Lu
Summary: In animals, personalities have been found to be linked to behavioral traits, health and disease susceptibility, and welfare. This study examines the performance differences of flounder offspring with different personalities (OFDP). The offspring of bold individuals (OB) were found to be heavier and have higher survival rates compared to the offspring of shy individuals (OS). The study also analyzes the variability of microsatellites and their relationship with OFDP, and finds that certain microsatellite loci are correlated with growth, heat tolerance, and disease resistance. These findings contribute to olive flounder aquaculture and provide insights for fish personality selective breeding.
Article
Biochemistry & Molecular Biology
Toshiya Yamaguchi, Takeshi Kitano
Summary: Japanese flounder is a teleost fish with an XX/XY sex determination system and temperature-dependent sex determination. The up-regulation of Amh expression during the sex differentiation period leads to masculinization. In this study, researchers found that the loss of Amhr2 function caused male-to-female sex reversal, confirming the role of Amh/Amhr2 signaling in inhibiting estrogen synthesis for masculinization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
D. P. Nagahawatta, N. M. Liyanage, Thilina U. Jayawardena, H. H. A. C. K. Jayawardhana, Jae-Young Oh, K. K. Asanka Sanjeewa, Sang In Kang, You-Jin Jeon
Summary: The study found that digested flounder meat has the potential to promote skeletal muscle growth and can be used as a functional food ingredient.
Article
Biochemistry & Molecular Biology
Hongfei Tian, Jing Xing, Xiaoqian Tang, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Summary: In this study, the activation of IL-2 on flounder lymphocytes was investigated, and the effects on B cell immunity after blocking IL-2 receptors (IL-2R beta or IL-2R gamma) were further examined. The differentially expressed genes in lymphocytes after IL-2 stimulation were found to be related to the immune response of B cells. IL-2 stimulation increased the percentages of mIgM+ B cells and enhanced their antibody secretion and phagocytosis, but these effects were significantly inhibited after blocking IL-2 receptors, especially IL-2R beta.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Immunology
Qian Li, Heng Chi, Xueyan Shi, Qiujie Gan, Roy Ambli Dalmo, Yuan-yuan Sun, Xiaoqian Tang, Jing Xing, Xiuzhen Sheng, Wenbin Zhan
Summary: This study introduced a new method for detecting ETs in the peritoneum of fish in vivo and indicated that ET formation is involved in the immune response at the early stage after intraperitoneal immunization to vaccines.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Wenjing Liu, Jing Xing, Xiaoqian Tang, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Summary: This study identified a CD80/86 homolog in flounder and investigated its expression characteristics in immune responses. The study revealed that CD80/86 is mainly found in antigen-presenting cells and participates in eliciting humoral immune responses through the CD80/86-CD28 signaling pathway.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Jing Xing, Wenjing Liu, Xiaoqian Tang, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Summary: CD28 is cloned and characterized in flounder, with a high expression in various tissues. CD28+ cells are co-localized with CD4+ T lymphocytes, not with IgM+ B lymphocytes. Stimulation with keyhole limpet hemocyanin induces significant changes in CD28 expression and leads to increased cell proliferation and IL-2 production. Anti-CD28 Abs enhance T cell activation and modulate gene expression in cultured leukocytes.
FRONTIERS IN IMMUNOLOGY
(2021)