Article
Biochemical Research Methods
Oren Avram, Aya Kigel, Anna Vaisman-Mentesh, Sharon Kligsberg, Shai Rosenstein, Yael Dror, Tal Pupko, Yariv Wine
Summary: The proteomics of serum antibodies (Ig-Seq) combines BCR-Seq and high-resolution mass-spectrometry, providing a comprehensive characterization of the humoral response. The PASA web server offers a robust computational platform for analyzing and integrating data from proteomics of serum antibodies, making it accessible to non-expert users.
PLOS COMPUTATIONAL BIOLOGY
(2021)
Article
Microbiology
Caelan E. Radford, Philipp Schommers, Lutz Gieselmann, Katharine H. D. Crawford, Bernadeta Dadonaite, Timothy C. Yu, Adam S. Dingens, Julie Overbaugh, Florian Klein, Jesse D. Bloom
Summary: Understanding the specificities of human serum antibodies that neutralize HIV can inform prevention and treatment strategies. A deep mutational scanning system was developed to measure the effects of mutations to HIV envelope on antibody neutralization. The study found that different polyclonal serum show neutralizing activity against HIV by targeting various epitopes, similar to characterized monoclonal antibodies. Mapping the specificity of neutralizing activity in polyclonal serum will help in evaluating anti-HIV immune responses for prevention strategies.
CELL HOST & MICROBE
(2023)
Article
Cell Biology
Inbal Farkash, Tali Feferman, Noy Cohen-Saban, Yahel Avraham, David Morgenstern, Grace Mayuni, Natasha Barth, Yaniv Lustig, Liron Miller, Dror S. Shouval, Asaf Biber, Ilya Kirgner, Yishai Levin, Rony Dahan
Summary: mRNA-based COVID-19 vaccines induce strong anti-SARS-CoV-2 antibody responses with unique and dynamic Fc compositions. The vaccine-induced antibodies exhibit differences in Fab and Fc-mediated functions between age groups and natural infection-generated antibodies. These findings suggest heterogeneity in Fc responses to SARS-CoV-2 infection and vaccination may support long-lasting protection differently.
Article
Immunology
Manuel Saez Moya, Rebeca Gutierrez-Cozar, Joan Punet-Ortiz, Maria Luisa Rodriguez de la Concepcion, Julia Blanco, Jorge Carrillo, Pablo Engel
Summary: In a study using NOD.H-2 (h4) mice as a model for spontaneous SjS, it was found that these mice have a higher frequency of self-reactive B-cell clones, and with age, there is an increase in polyreactive and autoreactive IgG clones. Furthermore, in these mice, there is a trend towards oligoclonality in IgG B cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Fariza Mishaal Saiema Badloe, Shauni De Vriese, Tina De Bruyn Carlier, Elise Vandersichel, Joerg Scheffel, Marcus Maurer, Johannes Ring, Jan Gutermuth, Inge Kortekaas Krohn
Summary: In this study, a novel method for the purification of total IgE from human serum was established using specific antibodies and beads, resulting in high purity of IgE. This highly effective and widely accessible approach can improve the use of IgE-based models and contribute to allergy research.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Tianling Ou, Wenhui He, Brian D. Quinlan, Yan Guo, Mai H. Tran, Pabalu Karunadharma, Hajeung Park, Meredith E. Davis-Gardner, Yiming Yin, Xia Zhang, Haimin Wang, Guocai Zhong, Michael Farzan
Summary: In this study, a diverse B cell repertoire that recognizes a key HIV-1 neutralizing epitope was generated by replacing the heavy-chain CDR3 region of human B cells. The edited B cells efficiently bound trimeric HIV-1 antigens, suggesting their potential for in vivo affinity maturation.
Article
Chemistry, Analytical
Karen A. M. de Jong, Hilde Rosing, Marit Vermunt, Alwin D. R. Huitema, Jos H. Beijnen
Summary: This study developed a quantitative serological test for anti-SARS-CoV-2 antibodies in human serum using LC-QTOF-MS, allowing precise quantification of IgG1 levels. The method was successfully applied to PCR-positive patients and those who recently recovered from COVID-19.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Chemistry, Applied
Cassie R. Bakshani, Fiona Cuskin, Neil J. Lant, Hamish C. L. Yau, William G. T. Willats, J. Grant Burgess
Summary: This study used molecular probe-based technologies to investigate the composition and relative abundance of glycans in burnt-on/baked-on (BoBo) food soil and identify enzymes that can facilitate soil breakdown. The results suggest that inclusion of xylan depolymerising enzymes in automatic dishwashing detergents may improve the cleaning of recalcitrant, plant glycan-rich BoBo soils. This study provides new insights into the composition and removal chemistry of complex, multi-component food soils.
Article
Chemistry, Multidisciplinary
Lun Bian, Qiangqiang Fu, Zhuoheng Gan, Ze Wu, Yuchen Song, Yufeng Xiong, Fang Hu, Lei Zheng
Summary: In this study, a fluorescence-quenching lateral flow immunoassay (FQ-LFIA) was developed for rapid and quantitative detection of anti-SARS-CoV-2 neutralizing antibodies (NAbs) in human serum. The FQ-LFIA showed excellent sensitivity and accuracy, and has the potential to be an effective method for detecting anti-SARS-CoV-2 NAbs.
Article
Biochemical Research Methods
Rafael D. Melani, Benjamin J. Des Soye, Jared O. Kafader, Eleonora Forte, Michael Hollas, Voislav Blagojevic, Fernanda Negrao, John P. McGee, Bryon Drown, Cameron Lloyd-Jones, Henrique S. Seckler, Jeannie M. Camarillo, Philip D. Compton, Richard D. LeDuc, Bryan Early, Ryan T. Fellers, Byoung-Kyu Cho, Basil Baby Mattamana, Young Ah Goo, Paul M. Thomas, Michelle K. Ash, Pavan P. Bhimalli, Lena Al-Harthi, Beverly E. Sha, Jeffrey R. Schneider, Neil L. Kelleher
Summary: Ig-MS is a novel serological readout capturing the immunoglobulin repertoire at molecular resolution without sequencing of B cells. This method can be used to assess exposure or immunity to a pathogen.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Cell Biology
Ivan Vujkovic-Cvijin, Hugh C. Welles, Connie W. Y. Ha, Lutfi Huq, Shreni Mistry, Jason M. Brenchley, Giorgio Trinchieri, Suzanne Devkota, Yasmine Belkaid
Summary: This study reports that certain commensal gut microbiota can translocate across the gut barrier in mice and humans and are associated with heightened systemic immune response. A modified high-throughput approach was developed to quantify systemic immune response against gut commensal bacteria in human serum samples without the need for paired stool samples. Several commensal bacterial species were found to elicit elevated immune response in patients with inflammatory bowel disease (IBD) and exhibited increased transcriptional activity and growth rates in IBD patient gut microbiomes. This approach serves as a complementary tool to understand the interactions between host and gut microbiota and may help identify microbes associated with inflammatory diseases.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
Taylor S. Cohen, Elizabeth J. Kelly, Sven Nylander, Himanshu Bansal, Brett M. Jepson, Prakash Bhuyan, Magdalena E. Sobieszczyk, Ann R. Falsey
Summary: Rare cases of thrombosis with thrombocytopenia syndrome (TTS) have been reported after AZD1222 vaccination. A study analyzed anti-PF4 levels in paired samples from a phase 3 trial of AZD1222 vs placebo. The results indicate that AZD1222 does not induce a clinically relevant general increase in anti-PF4 IgG.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Organic
Nives Hribernik, Fabrizio Chiodo, Roland J. Pieters, Anna Bernardi
Summary: Recruiting natural antibodies to eliminate tumor cells is a highly specific and effective therapeutic strategy. This study reports the first rhamnose-based glycomimetic used for this purpose, which captures anti-rhamnose antibodies.
TETRAHEDRON LETTERS
(2022)
Article
Cell Biology
Benjamin Motais, Sandra Charvatova, Zuzana Walek, Matous Hrdinka, Ryszard Smolarczyk, Tomasz Cichon, Justyna Czapla, Sebastian Giebel, Michal Simicek, Tomas Jelinek, Tereza Sevcikova, Jiri Sobotka, Zdenek Koristek, Roman Hajek, Juli R. Bago
Summary: Cellular immunotherapy has shown promising results in cancer treatment, but faces challenges such as off-tumor toxicity and tumor recurrence. A study aimed to utilize NK cells' anti-tumor characteristics to target a wide range of cancers, combining them with the anti-CD-38 antibody Daratumumab for a synergistic effect against multiple myeloma, showing a significant reduction in tumor volume in mouse models.
Review
Medicine, General & Internal
Michal Braczkowski, Dariusz Soszynski, Alicja Sierakowska, Ryszard Braczkowski, Klaudia Kufel, Beata Labuz-Roszak
Summary: Encephalitis is a complex condition with various causes and symptoms. Autoimmune encephalitis, which accounts for about 4% of encephalitis cases, is caused by autoantibodies attacking the central nervous system. This paper discusses the mental and neurological symptoms of autoimmune encephalitis, as well as the coexistence with psychosis and neoplastic diseases. Early diagnosis and targeted treatment are emphasized for a successful therapeutic process. The research methodology involved searching for articles from 2007 to 2023 using search engines like PubMed and Medline.
Article
Pharmacology & Pharmacy
Junhua Wang, Stephan von Gunten, Guido Beldi, Denis Grandgirard, Stephen L. Leib, Bruno Gottstein
Summary: The study demonstrated that the parasite vesicular structure could be directly destroyed by adding galactosidases into the in vitro culture system, leading to the inability of the parasite metacestode vesicles to infect mice. Pretreatment of E. multilocularis metacestode with beta 1-3-galactosidase resulted in upregulated PD1 expression and lower IL-10 secretion in CD4(+) Teffs from inoculated mice, while there was no significant difference in PD1 and IL-10 expression levels in CD4(+) Teffs from mice inoculated with E. multilocularis metacestode pretreated with alpha 1-3,4,6-galactosidase.
Article
Allergy
Paul Engeroff, Kevin Plattner, Federico Storni, Franziska Thoms, Kayluz Frias Boligan, Lukas Muerner, Alexander Eggel, Stephan von Gunten, Martin F. Bachmann, Monique Vogel
Summary: The study demonstrates that glycan-specific IgG anti-IgE autoantibodies can downregulate serum IgE levels and anaphylactic activity, providing potential therapeutic implications for allergic diseases.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kathrin Stavenhagen, Lisa C. Laan, Chao Gao, Akul Y. Mehta, Jamie Heimburg-Molinaro, Jonathan N. Glickman, Irma van Die, Richard D. Cummings
Summary: The macrophage mannose receptor (CD206, MR) shows high selectivity for specific types of pauci- and oligomannose N-glycans, which are often elevated in tumor cells, implying a potential role in tumor biology. The presence of MR glycoprotein ligands on cancer cells suggests a specific binding pattern that could be significant in tumor biology. Further analysis confirms the selective recognition of pauci- and oligomannose N-glycans by the MR, indicating a potential role in tumor biology.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Multidisciplinary Sciences
Chao Gao, Kathrin Stavenhagen, Barbara Eckmair, Tanya R. McKitrick, Akul Y. Mehta, Yasuyuki Matsumoto, Alyssa M. McQuillan, Melinda S. Hanes, Deniz Eris, Kelly J. Baker, Nan Jia, Mohui Wei, Jamie Heimburg-Molinaro, Beat Ernst, Richard D. Cummings
Summary: The study investigates the recognition of oligomannose-type glycans in innate and adaptive immunity, developing a multifaceted approach to analyze glycan structures and create a comprehensive oligomannose microarray. The results show that different proteins exhibit unique specificity towards oligomannose motifs.
Article
Immunology
Babette Wolf, Mateusz Piksa, Isabelle Beley, Agnes Patoux, Thierry Besson, Valerie Cordier, Bernd Voedisch, Patrick Schindler, Daniela Stoellner, Ludovic Perrot, Stephan von Gunten, Dominique Brees, Michael Kammueller
Summary: This study demonstrates the relevance of glycosylation in immune recognition of therapeutic antibodies, and the ability of defined glycan structures to modulate immunogenicity and anti-drug antibody responses. Glyco-engineering may be a useful strategy to reduce clinical immunogenicity and adverse events associated with antibodies.
Editorial Material
Pharmacology & Pharmacy
Guy Gorochov, Stephan von Gunten
Article
Immunology
Morten Aagaard Nielsen, Kristian Juul-Madsen, John Stegmayr, Chao Gao, Akul Y. Mehta, Stinne Ravn Greisen, Tue Wenzel Kragstrup, Malene Hvid, Thomas Vorup-Jensen, Richard D. Cummings, Hakon Leffler, Bent Winding Deleuran
Summary: The study shows that Gal-3 binds to 4-1BB and forms large soluble 4-1BB/Gal-3 complexes that attach to mem4-1BB on the cell surfaces, leading to suppression of 4-1BBL's bioactivity. In rheumatoid arthritis patients, the complexes containing 4-1BB/Gal-3 were significantly reduced.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kathrin Stavenhagen, Akul Y. Mehta, Lisa Laan, Chao Gao, Jamie Heimburg-Molinaro, Irma van Die, Richard D. Cummings
Summary: The macrophage mannose receptor (MR) is involved in immunity and maintaining homeostasis. The glycans within MR act as a regulatory switch by masking and unmasking self-ligands, controlling the receptor's activity.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Immunology
Quentin Haas, Nikita Markov, Lukas Muerner, Viviana Rubino, Andrej Benjak, Monika Haubitz, Gabriela M. Baerlocher, Charlotte K. Y. Ng, Christian Munz, Carsten Riether, Adrian F. Ochsenbein, Hans-Uwe Simon, Stephan von Gunten
Summary: This study investigates the expression and function of inhibitory Siglec receptors in lymphocyte subsets. The researchers discover Siglec-7 as a glyco-immune checkpoint expressed on non-exhausted effector memory CD8+ T cells, regulating their functional and metabolic capacities. They also find that Siglec-7 polarization into the T cell immune synapse depends on sialoglycan interactions, which prevent actin polarization and effective T cell responses. Siglec-7 ligands are observed on leukemic stem cells and acute myeloid leukemia (AML) cells, suggesting the potential therapeutic role of Siglec-7 in T cell-driven disorders and cancer.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Pharmacology & Pharmacy
Ruqayyah J. Almizraq, Kayluz Frias Boligan, Bonnie J. B. Lewis, Selena Cen, Heather Whetstone, Rolf Spirig, Fabian Kasermann, Ian K. Campbell, Stephan von Gunten, Donald R. Branch
Summary: The study investigated the potential anti-inflammatory effect of rFc-mu TP-L309C on neutrophils in the K/BxN mouse model of rheumatoid arthritis. The results showed that rFc-mu TP-L309C treatment significantly ameliorated arthritis symptoms in K/BxN mice by reducing neutrophil infiltration and IL-1 beta production. This suggests that rFc-mu TP-L309C may be a potential therapeutic candidate for neutrophil-mediated autoimmune and inflammatory conditions.
Article
Cell Biology
Morten A. A. Nielsen, Ditte Koster, Akul Y. Y. Mehta, Kristian Stengaard-Pedersen, Pierre Busson, Peter Junker, Kim Horslev-Petersen, Merete Lund Hetland, Mikkel ostergaard, Malene Hvid, Hakon Leffler, Tue W. W. Kragstrup, Richard D. D. Cummings, Bent Deleuran
Summary: This study evaluated the clinical and pathogenic aspects of Gal-9 in rheumatoid arthritis (RA) and found that it plays a role in modulating synovial FLS activities and maintaining subclinical disease activity.
Letter
Allergy
Kevin Plattner, Gilles Augusto, Lukas Muerner, Stephan von Gunten, Lukas Jorg, Paul Engeroff, Martin. F. Bachmann, Monique Vogel
Article
Chemistry, Multidisciplinary
Cangjie Yang, Yu Deng, Yang Wang, Chaoshuang Xia, Akul Y. Mehta, Kelly J. Baker, Anuj Samal, Putthipong Booneimsri, Chanthakarn Lertmaneedang, Seung Hwang, James P. Flynn, Muqing Cao, Chao Liu, Alec C. C. Zhu, Richard D. Cummings, Cheng Lin, Udayan Mohanty, Jia Niu
Summary: In this study, a new approach to HS glycomimetics was developed by iteratively assembling clickable disaccharide building blocks. The HS-mimetic oligomers with defined sulfation patterns were shown to bind protein FGF2 in a sulfation-dependent manner, indicating their potential application as alternatives to native HS in both fundamental research and disease models.
Review
Pharmacology & Pharmacy
Caroline von Achenbach, Giselle Hevia Hernandez, Stephan von Gunten
Summary: IVIG and SCIG are used to treat various immunodeficiencies and inflammatory conditions, with treatment decisions influenced by patient conditions, preferences, and economic factors. Understanding and comparing the two treatment modalities are crucial for optimal therapeutic options.