Article
Physiology
Zhen Chen, Yating Xu, Dupeng Ma, Changrong Li, Ziqi Yu, Cong Liu, Tingyu Jin, Ziye Du, Zejia Li, Qi Sun, Yumin Xu, Rong Liu, Yuerong Wu, Mengcheng Luo
Summary: Cep72 is a critical component of the centrosome, participating in regulating sperm morphology, but it is dispensable for fertility in mice.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Cell Biology
Qian Ma, Congcong Cao, Changshui Zhuang, Xiaomin Luo, Xiaofeng Li, Huijuan Wan, Jing Ye, Fangfang Chen, Lina Cui, Yan Zhang, Yujiao Wen, Shuiqiao Yuan, Yaoting Gui
Summary: AXDND1, a novel testis-enriched gene, is essential for spermiogenesis and male fertility. Knockout of AXDND1 leads to infertility, reduced testis size, abnormal sperm morphology, and structural defects in spermatozoa.
CELL DEATH DISCOVERY
(2021)
Article
Cell Biology
Amanda Guerreiro, Filipe De Sousa, Nicolas Liaudet, Daria Ivanova, Anja Eskat, Patrick Meraldi
Summary: In cells, WDR62 localizes at spindle poles, promoting the recruitment of the microtubule-severing enzyme katanin. This protein helps in efficient poleward microtubule flux during chromosome segregation, ultimately preventing lagging chromosomes during anaphase.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Cell Biology
Melis D. Arslanhan, Seyma Cengiz-Emek, Ezgi Odabasi, Emmanuelle Steib, Virginie Hamel, Paul Guichard, Elif Nur Firat-Karalar
Summary: Our research highlights the role of CCDC15 in regulating centriole size, integrity, and cilium formation, providing insights into diseases associated with centriolar defects.
JOURNAL OF CELL BIOLOGY
(2023)
Review
Microbiology
Scott M. Landfear
Summary: This review highlights recent advances in understanding the flagellar function in various biological contexts encountered by Leishmania parasites.
Article
Cell Biology
Pablo Lopez-Jimenez, Sara Perez-Martin, Ines Hidalgo, Francesc R. Garcia-Gonzalo, Jesus Page, Rocio Gomez
Summary: Cilia play important roles in the airways and reproductive system as motile cilia beat to displace extracellular fluids, while primary cilia function as sensory organelles detecting signals from the external environment. Dysfunction of cilia is associated with genetic diseases and some types of cancer. In mouse meiosis, solitary cilia were found in zygotene spermatocytes, suggesting a potential sensory role affecting cyst function during prophase I.
Article
Cell Biology
Sandrine Floriot, Laura Bellutti, Johan Castille, Pauline Moison, Sebastien Messiaen, Bruno Passet, Laurent Boulanger, Abdelhak Boukadiri, Sophie Tourpin, Christian Beauvallet, Marthe Vilotte, Julie Riviere, Christine Pechoux, Maud Bertaud, Jean-Luc Vilotte, Gabriel Livera
Summary: This study reveals the essential role of centrosome cohesion in male gametogenesis. Mutant mice lacking the Cep250 gene exhibit male infertility due to a reduction in the spermatogonial pool and the blockade of meiotic divisions. The findings provide new insights into the function of centrosome cohesion in male germline development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Jae Yeon Hwang, Shoaib Nawaz, Jungmin Choi, Huafeng Wang, Shabir Hussain, Mehboob Nawaz, Francesc Lopez-Giraldez, Kyungjo Jeong, Weilai Dong, Jong-Nam Oh, Kaya Bilguvar, Shrikant Mane, Chang-Kyu Lee, Christopher Bystroff, Richard P. Lifton, Wasim Ahmad, Jean-Ju Chung
Summary: A novel DNAH2 gene variant was found to be closely associated with asthenozoospermia, and in mouse experiments, it was confirmed that DNAH2 deficiency leads to male infertility and affects sperm flagella development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Cell Biology
Shenae L. Cafe, Brett Nixon, Heath Ecroyd, Jacinta H. Martin, David A. Skerrett-Byrne, Elizabeth G. Bromfield
Summary: Protein homeostasis is crucial for the function and viability of fully differentiated, long-lived cells, including neurons and germ cells. Understanding and maintaining protein homeostasis is vital for successful fertilization and reproductive health. This review highlights the importance of proteostasis in germline cells and its potential impact on aging and fertility.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Developmental Biology
Yi Tian Yap, Lin Shi, David Zhang, Qian Huang, Fabiha Siddika, Zhenyu Wang, Wei Li, Zhibing Zhang
Summary: Sperm flagella formation requires the participation of two cargo transport systems, intramanchette transport (IMT) and intraflagellar transport (IFT). Our study found that MEIG1, IFT20, and IFT88 proteins have the same localization in elongating spermatids, and are crucial for normal sperm development and flagella formation. Co-immunoprecipitation assay supports the hypothesis that these three proteins are in the same complex. Additionally, MEIG1 plays a key role in determining the manchette localization of certain IFT components in male germ cells.
DEVELOPMENTAL BIOLOGY
(2022)
Article
Plant Sciences
Jia Shu, Xiaochang Yin, Yannan Liu, Yingjie Mi, Bin Zhang, Aoyuan Zhang, Hongbo Guo, Juane Dong
Summary: DNA methylation is important for epigenetic modifications and AtMBD3 is highly expressed in pollen and seeds. Two mutant alleles of AtMBD3, mbd3-1 and mbd3-2, were obtained using CRISPR/Cas9 and showed decreased pollen production and seed abortion. Transcriptome analysis revealed significant differences in gene expression between wild type and mbd3-2 mutants, including genes involved in embryo development. PBL6, a gene specifically expressed in pollen, was found to interact with AtMBD3. These findings provide insights into the function of AtMBD3 in plant fertility.
Review
Endocrinology & Metabolism
Juho-Antti Makela, Jorma Toppari
Summary: The RB protein family members play crucial roles in cell cycle progression, apoptosis, and stem cell self-renewal and differentiation. RB proteins exert their effects through interaction with E2F transcription factors. RB and E2F family members are widely and dynamically expressed in the testis and have versatile roles during spermatogenesis. RB-E2F interaction is critical for male fertility and proper germline maintenance and lifelong sperm production.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Tiina Lehtiniemi, Matthieu Bourgery, Lin Ma, Ammar Ahmedani, Margareeta Makela, Juho Asteljoki, Opeyemi Olotu, Samuli Laasanen, Fu-Ping Zhang, Kun Tan, Jennifer N. Chousal, Dana Burow, Satu Koskinen, Asta Laiho, Laura L. Elo, Frederic Chalmel, Miles F. Wilkinson, Noora Kotaja
Summary: This study demonstrates that SMG6 is essential for male germ cell differentiation in mice, and its loss leads to transcriptome misregulation and arrest of spermatogenesis. SMG6 is enriched in the chromatoid body and may function together with the piRNA pathway to co-regulate genes in round spermatids.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Immunology
Lena Roling, Ansgar Flammersfeld, Gabriele Pradel, Sandra Bennink
Summary: The WD40-repeat protein PfWLP1 plays a crucial role in interacting with adhesion protein complexes in the malaria parasite, essential for the stability and maturation of gametocytes, affecting exflagellation ability.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Obstetrics & Gynecology
Meiqi Hou, Qingsong Xi, Lixia Zhu, Weimin Jia, Zhenxing Liu, Cheng Wang, Xiaopei Zhou, Dazhi Zhang, Chenxi Xing, Xuejie Peng, Yalin Luo, Lei Jin, Zhou Li, Xianqin Zhang
Summary: Multiple morphological abnormalities of the sperm flagella (MMAF) are an important basis for male infertility, with genetic factors playing a significant role. A novel compound heterozygous mutation in the FSIP2 gene was found in an infertile male patient with MMAF. The study confirms the impact of this mutation on sperm through histopathological staining, electron microscopy observation, and immunofluorescence analysis. These findings contribute to the precise diagnosis of MMAF and male infertility.
REPRODUCTIVE SCIENCES
(2022)