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Evaluation in Swine of a Recombinant African Swine Fever Virus Lacking the MGF-360-1L Gene

VIRUSES-BASEL (2020)

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期刊

VIRUSES-BASEL
卷 12, 期 10, 页码 -

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MDPI
DOI: 10.3390/v12101193

关键词

ASFV; ASF; African swine fever virus; MGF360-1L

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Virology

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  1. Science and Technology Directorate of the U.S. Department of Homeland Security [70RSAT19KPM000056]

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The African swine fever (ASF) pandemic is currently affecting pigs throughout Eurasia, resulting in significant swine production losses. The causative agent, ASF virus (ASFV), is a large, structurally complex virus with a genome encoding more than 160 genes. The function of most of those genes remains unknown. Here, we presented the previously uncharacterized ASFV gene MGF360-1L, the first gene in the genome. The kinetic studies of virus RNA transcription demonstrated that the MGF360-1L gene was transcribed as a late virus protein. The essentiality of MGF360-1L to virus replication was evaluated by developing a recombinant ASFV lacking the gene (ASFV-G-Delta MGF360-1L). In primary swine macrophage cell cultures, ASFV-G-Delta MGF360-1L showed similar replication kinetics as the parental highly virulent field isolate Georgia2007 (ASFV-G). Domestic pigs experimentally infected with ASFV-G-Delta MGF360-1L presented with a clinical disease indistinguishable from that caused by ASFV-G, demonstrating that MGF360-1L was not involved in virulence in swine, the natural host of ASFV.

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