期刊
VIRUSES-BASEL
卷 12, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/v12101060
关键词
T7-like phage; antibiotic resistance genes (ARG); potential packaging; phage-host interaction
类别
资金
- Major Scientific Research Projects of Guangdong Provincial [2017KZDXM006]
The increase in antimicrobial resistance is a threat to both human and animal health. The transfer of antibiotic resistance genes (ARG) via plasmids has been studied in detail whereas the contribution of bacteriophage-mediated ARG transmission is relatively little explored. We isolated and characterized two T7-like lytic bacteriophages that infected multidrug-resistant Escherichia coli hosts. The morphology and genomic analysis indicated that both phage HZP2 and HZ2R8 were evolutionarily related and their genomes did not encode ARGs. However, ARG-like raw reads were detected in offspring sequencing data with a different abundance level implying that potential ARG packaging had occurred. PCR results demonstrated that six fragments of genes (qnrS, cmlA, tetM, bla(TEM), sul3, mcr-1) were potentially packaged by phage HZP2 and four (qnrS, cmlA, bla(TEM), mcr-1) by phage HZ2R8. Further quantitative results showed that ARG abundance hierarchies were similar. The gene bla(TEM) was the most abundant (up to 1.38 x 10(7) copies/mL) whereas cmlA and qnrS were the least. Moreover, the clinically important mcr-1 gene was the second most abundant ARG indicating a possibility for spread through generalized transduction. Together, our results indicated that these structurally similar phage possessed similar characteristics and potential packaging during phage-host interaction displayed an ARG preference rather than occurring randomly.
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