Article
Environmental Sciences
Thitima Srathongneam, Montakarn Sresung, Phongsawat Paisantham, Pacharaporn Ruksakul, Andrew C. Singer, Rojana Sukchawalit, Jutamaad Satayavivad, Skorn Mongkolsuk, Kwanrawee Sirikanchana
Summary: This study analyzes the prevalence of antibiotic resistance genes (ARGs) in various water environments in Thailand using high-throughput qPCR. The study reveals a widespread presence of ARGs in different water types, suggesting a circulation of resistance determinants across the aquatic environment. The study also highlights the higher abundance of ARGs in effluent and river water, indicating possible inadequate wastewater treatment and environmental contamination. Seasonal impacts on wastewater treatment practices and pollution patterns are also observed.
SCIENCE OF THE TOTAL ENVIRONMENT
(2024)
Article
Engineering, Environmental
Barbara Drigo, Gianluca Brunetti, Samuel C. Aleer, Jan M. Bell, Michael D. Short, Sotirios Vasileiadis, John Turnidge, Paul Monis, David Cunliffe, Erica Donner
Summary: With two thirds of the global population living in areas affected by water scarcity, wastewater reuse is actively being implemented or explored by many nations. The study used probe-based multiplex and real-time PCR assays to monitor pathogen and antimicrobial resistance gene abundance and activity in recycled water. Results showed that recycled water treatment plants effectively removed over 95% of pathogens and antimicrobial resistance genes, but some subpopulations survived disinfection, highlighting the need for further research on the persistence and growth of these microorganisms in recycled water systems.
Article
Engineering, Environmental
Ji Lu, Zhigang Yu, Pengbo Ding, Jianhua Guo
Summary: Triclosan significantly enhances the conjugative transfer of the RP4 plasmid among activated sludge communities, promoting both intergenera and intragenera transfer. Triclosan also facilitates the transfer of the RP4 plasmid to opportunistic human pathogens. The mechanism behind triclosan-mediated conjugative transfer involves oxidative stress, increased membrane permeability, and the activation of the SOS response.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Engineering, Environmental
Chengsong Ye, Yuming Chen, Lin Feng, Kun Wan, Jianguo Li, Mingbao Feng, Xin Yu
Summary: This study investigated the occurrence of pathogens, antibiotic resistance genes (ARGs), and bacterial communities in UV/chlorine-treated reclaimed water samples. The results showed that UV/chlorine treatment effectively reduced the numbers of pathogens and changed the bacterial community structure. Additionally, the treatment decreased the abundance of ARGs and mobile genetic elements (MGEs). However, certain ARGs and MGEs were consistently detected across different seasons, indicating the need for attention.
FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING
(2022)
Article
Environmental Sciences
Simon Bo Lassen, Md Emranul Ahsan, Seikh Razibul Islam, Xin-Yuan Zhou, Muhammad Abdur Razzak, Jian-Qiang Su, Kristian Koefoed Brandt
Summary: The study revealed a significant presence of ARGs in commercial aquaculture ponds in Bangladesh, with multidrug resistance genes being the most common. Research ponds managed by the Bangladesh Agricultural University showed the lowest abundance and diversity of ARGs. The composition of ARGs displayed a distinct biogeographical pattern, potentially influenced by different feed types.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Public, Environmental & Occupational Health
Isaac Raplee, Lacey Walker, Lei Xu, Anil Surathu, Ashok Chockalingam, Sharron Stewart, Xiaomei Han, Rodney Rouse, Zhihua Li
Summary: The study aimed to confirm gut microbiota shifts in an animal model of antibiotic treatment and found that antibiotic therapy reduced detectable species of bacteria and increased opportunistic pathogens in the gut microbiota. Furthermore, antibiotic treatment selected for antibiotic-resistant gene enriched subpopulations for many of these pathogens.
ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL
(2021)
Article
Multidisciplinary Sciences
Karina-Doris Vihta, Nicola Claire Gordon, Nicole Stoesser, T. Phuong Quan, Carina S. B. Tyrrell, Manivanh Vongsouvath, Elizabeth A. Ashley, Vilada Chansamouth, Paul Turner, Clare L. Ling, David W. Eyre, Nicholas J. White, Derrick Crook, Tim E. A. Peto, Ann Sarah Walker
Summary: The study found that antimicrobial resistance in commensal opportunistic pathogens cultured from BSIs is strongly associated with resistance in commensal opportunistic pathogens cultured from non-sterile sites over calendar time, suggesting the latter could be used as an effective proxy for antimicrobial resistance surveillance in BSIs. The results were consistent across multiple countries in different income settings, indicating the potential for broader application in diverse healthcare settings.
SCIENTIFIC REPORTS
(2021)
Article
Environmental Sciences
Yuqing Mao, Neslihan Akdeniz, Thanh H. Nguyen
Summary: This study compared the abundances of genetic markers of opportunistic foodborne and airborne pathogens in backyard, commercial, and swine mortality composts. The findings suggest that backyard composts have lower abundances of antibiotic resistance genes, while airborne pathogen genes are present in various compost samples to some extent.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Engineering, Environmental
Junya Zhang, Tiedong Lu, Hui Zhong, Peihong Shen, Yuansong Wei
Summary: The study found that using zero valent iron (ZVI) can simultaneously improve methane production and reduce antibiotic resistance genes (ARGs). ZVI specifically facilitated the reduction of antibiotic resistance genes, possibly through inhibiting enzyme activity and intracellular ionic disturbance.
Article
Engineering, Environmental
Ana Puljko, Milena Milakovic, Stela Krizanovic, Josipa Kosic-Vuksic, Ivana Babic, Ines Petric, Ana Maravic, Marko Jelic, Nikolina Udikovic-Kolic
Summary: Extended-spectrum beta-lactamase (ESBL) and carbapenemase-producing Enterobacterales are still present in the environment even after conventional treatment in wastewater treatment plants. Enteric opportunistic pathogens such as Klebsiella pneumoniae and Acinetobacter baumannii are efficiently removed, but Escherichia coli and Enterococcus spp. still remain in relatively high concentrations in the effluent. Improvement in wastewater treatment technologies is necessary to minimize the risk of environmental contamination and public health issues related to ESBL and carbapenemase-producing Enterobacterales.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Jakub Hubeny, Ewa Korzeniewska, Slawomir Ciesielski, Grazyna Plaza, Monika Harnisz
Summary: This study quantified ESKAPEE bacteria, antibiotic resistance genes, and integrase genes in municipal wastewater and river water. The presence of A. baumannii, E. coli, and resistance genes was detected downstream from the wastewater discharge point, posing a public health risk.
Article
Infectious Diseases
Maria-Theresia Gekenidis, Fiona Walsh, David Drissner
Summary: This study analyzed the contribution of surface and tap water to the resistome of overhead-irrigated chive plants and found that water quality significantly affects the resistome of irrigated produce. Water played a major role in antibiotic resistance gene enrichment, especially beta-lactam ARGs and MGEs. Various antibiotic resistance genes were identified in both water sources and chive plants, with bla(KPC) detected at high levels in both.
Article
Environmental Sciences
Yisong Weng, Yan Qiao, Changyan Xiao, Lijuan Feng
Summary: A novel underground reservoir was constructed in an abandoned tunnel for preserving water in a remote island. The microbial community, potential opportunistic pathogens (OPs), and antibiotic resistance genes (ARGs) in the water were analyzed over a year. The results showed that the culturability of total bacteria and coliforms in the water were sensitive to temperature, with a significant positive relationship between total bacteria culturability and temperature throughout the year.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH
(2022)
Article
Infectious Diseases
Federica Mauri, Elisa Pianta
Summary: This study monitored the presence of antibiotic resistance genes (ARGs) in water samples from a subalpine lake and rivers in Switzerland. The results showed that ARGs were present in the water environment and could potentially be transmitted to humans. The most abundant ARGs were sulII, followed by ermB, qnrS, and tetA, and there was an overall decrease in the number of resistance genes over the three-year monitoring period.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Biology
David Lund, Roelof Dirk Coertze, Marcos Parras-Molto, Fanny Berglund, Carl-Fredrik Flach, Anna Johnning, D. G. Joakim Larsson, Erik Kristiansson
Summary: This study uses computational screening to identify previously undiscovered antibiotic resistance genes in bacterial genomes, expanding the range of clinically relevant resistance determinants and demonstrating the early discovery potential of computational methods.
COMMUNICATIONS BIOLOGY
(2023)
Article
Engineering, Environmental
Xiao Zhang, Lili Zhang, Chun Hu, Min Yang
Summary: Highly dispersed LaAlO3/Al2O3 framework Cu+/Cu2+ catalyst was synthesized by a solid-liquid mixed sol-gel method, which showed efficient removal of refractory pollutants over a wide pH range.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Engineering, Chemical
Ming Su, Yiping Zhu, Tom Andersen, Xianyun Wang, Zhiyong Yu, Jinping Lu, Yichao Song, Tengxin Cao, Jianwei Yu, Yu Zhang, Min Yang
Summary: This study investigates the relationships between two filamentous cyanobacteria in a drinking water reservoir and finds that light is the major driving force responsible for the succession between these cyanobacteria. Subtle niche differentiation may play an important role in shaping the cyanobacterial assemblages, leading to MIB odor problems in drinking water reservoirs.
Article
Environmental Sciences
Haodi Feng, Mei Tang, Ziming Han, Xiao Luan, Chunmeng Ma, Min Yang, Jiuyi Li, Yu Zhang
Summary: An accurate analytical method was developed to quantify erythromycin A, byproducts, and hydrolysis or biodegradation products in erythromycin fermentation residue (EFR) and amended soil. The method achieved effective extraction and high recoveries for all analytes. EFR, treated EFR, and amended soils were analyzed, and several analytes were detected. The method was useful for evaluating the potential risk of erythromycin A byproducts and transformation products in the environment.
Article
Environmental Sciences
Jinyi Qin, Jiao Fang, Fei Gao, Yiwen He, Ming Su, Yu Zhang, Min Yang
Summary: This study investigated the treatment of Ethinyl Estradiol (EE2) in swine wastewater using an Upstream Solids Reactor (USR), and proposed a new method for bio-feeding regulation to enhance the anaerobic biodegradation process. The addition of propionic acid was found to be more effective in enhancing the removal of EE2 compared to lactic acid. However, controlling the pH at extreme values hindered the fermentation process and negatively affected EE2 removal. The study provides insights into the anaerobic biodegradation of EE2 and suggests a new strategy for the bioremediation of refractory pollutants.
Article
Engineering, Environmental
Ye Tian, Zhe Tian, Haodi Feng, Xiao Luan, Ziming Han, Yu Zhang, Min Yang
Summary: This study aimed to recover the nitrification performance of a full-scale pharmaceutical wastewater treatment system inhibited by high organic and oxytetracycline (OTC) loadings. Experimental results showed that reducing the organic loading rate (OLR) and influent OTC concentration led to an increase in ammonia-oxidizing bacteria and Nitrobacter, resulting in partial and then complete nitrification. The study also found that adsorbed OTC at a certain concentration could inhibit nitrification performance.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Environmental Sciences
Ming Su, Jiao Fang, Zeyu Jia, Yuliang Su, Yiping Zhu, Bin Wu, John C. Little, Jianwei Yu, Min Yang
Summary: Cyanobacteria can adjust their photosynthetic pigments to sense different light colors. Some filamentous cyanobacteria increase their phycoerythrin (PE) levels in green light, leading to extensive odor problems in drinking water reservoirs. This study found a correlation between MIB biosynthesis and chromatic acclimation (CA) induced by ambient light color in a MIB-producing Pseudanabaena strain. Pseudanabaena regulates pigment proportion through CA3, increasing PE and decreasing phycocyanin (PC) in green light. MIB production and chlorophyll a (Chl a) biosynthesis are positively correlated regardless of light color.
ENVIRONMENTAL RESEARCH
(2023)
Article
Environmental Sciences
Wenli Fan, Guangxi Sun, Qi Wang, Fan Yang, Yingxin Gao, Min Yang
Summary: This study investigated the role of activated carbon properties in the adsorption of effluent organic matter (EfOM) from bio-treated coking wastewater. Commercial powdered activated carbons (PACs) with different pore structures, surface functional groups, and surface charges were used. The results showed that the adsorption of EfOM depended on its molecular size, with biopolymers being adsorbed by macropores and humics being adsorbed by mesopores. PACs with higher mesopore volume, fewer acidic groups, and higher isoelectric point were found to be more effective in removing EfOM.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Engineering, Environmental
Tengxin Cao, Jiao Fang, Zeyu Jia, Yiping Zhu, Ming Su, Qi Zhang, Yichao Song, Jianwei Yu, Min Yang
Summary: Cellular 2-methylisoborneol (MIB) yield in cyanobacteria varies under different conditions, making MIB prediction ineffective. Through a field survey, we found that mic gene abundance and expression could serve as indicators for early warning of MIB production. Light intensity was identified as the key regulator for mic gene expression and MIB synthesis. We developed a model using mic gene abundance as an indicator for MIB prediction, which was verified in other reservoirs.
Article
Engineering, Environmental
Shiting Dai, Qing He, Ziming Han, Wenli Shen, Ye Deng, Yang Wang, Wei Qiao, Min Yang, Yu Zhang
Summary: The epicPCR method is developed to accurately identify and locate bacterial hosts carrying the tet(X4) gene, revealing a more diverse range of bacteria involved in harboring tet(X4) than previously realized.
Article
Marine & Freshwater Biology
Yanhong Shi, Chen Chen, Ziming Han, Kun Chen, Xiangyang Wu, Xuchun Qiu
Summary: This study investigates the combined effects of microplastics and commonly used antidepressants on the intestinal tissue and gut microbiota of zebrafish. The results show that exposure to the combination of microplastics and antidepressants leads to severe gut injuries, dysbiosis in the gut microbiota, and inflammation. These findings are important for assessing the combined effects of microplastics and tricyclic antidepressants on the health of aquatic organisms.
AQUATIC TOXICOLOGY
(2023)
Article
Engineering, Environmental
Tiantian Tian, Xiaoxiao Yang, Shihai Liu, Ziming Han, Wei Qiao, Jiuyi Li, Min Yang, Yu Zhang
Summary: The extensive use of florfenicol in poultry industry leads to the emergence of optrA gene, which confers resistance to linezolid. This study investigated the occurrence and removal of optrA in enterococci in anaerobic digestion systems and anaerobic pretreatment system for chicken waste. The optrA gene was frequently detected in enterococci from chicken waste and effluents from mesophilic and thermophilic reactors, but rarely detected in the hyper-thermophilic effluent. Hyper-thermophilic pretreatment removed enterococci with plasmid-borne optrA gene.
Article
Engineering, Environmental
Qiang Li, Lei Wang, Yingting Jia, Min Yang, Haifeng Zhang, Jianying Hu
Summary: Exposure to environmental endocrine-disrupting chemicals (EDCs) can cause extensive health issues. In this study, potential estrogen receptor alpha (ER alpha)-active compounds in the source and drinking water of major rivers in China were identified using an ER alpha protein affinity assay and high-resolution mass spectrometry. Fifty-one potential ER alpha-active compounds across 13 categories were identified, including a compound with antiestrogenic activity and three plausible ER alpha ligands. The study also revealed the presence of 12 compounds in the aquatic environment for the first time.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Article
Engineering, Environmental
Xinming Shen, Qiang Li, Wenxin Hu, Min Yang, Wei An, Jianying Hu
Summary: This study reveals that drinking water from six major cities in China can significantly promote foam cell formation in atherosclerosis, and chemicals in the water may play a role in this process by antagonizing liver X receptor alpha.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2023)
Review
Environmental Sciences
Hang Liu, Ziqi Li, Chao Liu, Zhimin Qiang, Tanju Karanfil, Min Yang
Summary: This review compares the elimination of intracellular and extracellular antibiotic resistance genes (ARGs) in representative disinfection processes applied at water and wastewater treatment plants. The study found that ozone had the highest elimination efficiency, followed by chlorine and chloramine. The elimination of extracellular ARGs could be up to 4-log higher than that of intracellular ARGs.
Article
Engineering, Environmental
Rong-Gui Zhu, Chang-Gui Pan, Feng-Jiao Peng, Chao-Yang Zhou, Jun-Jie Hu, Kefu Yu
Summary: This comprehensive survey investigated the occurrence, bioaccumulation, and trophic magnification of parabens and their metabolite 4-HB in a marine food web. Results showed that parabens were the predominant pollutants in marine organisms, with significant bioaccumulation from sediments. The estimated trophic magnification factor indicated biomagnification for MeP and trophic dilution for 4-HB. Overall, the risks for humans consuming marine organisms were found to be low.
Article
Engineering, Environmental
Andres F. Torres-Franco, Deborah Leroy-Freitas, Cristina Martinez-Fraile, Elisa Rodriguez, Pedro A. Garcia-Encina, Raul Munoz
Summary: Anaerobic and microalgae-based technologies have emerged as sustainable alternatives for municipal wastewater treatment. However, the presence of viruses in the treated wastewater is a major concern for reuse applications. This study assessed the ability of these technologies to reduce viruses during secondary wastewater treatment. The results showed that all technologies were effective in reducing the concentration of viruses, with microalgae-based treatment exhibiting the highest potential for reducing the disinfection requirements of treated wastewater.
Article
Engineering, Environmental
Young Gwang Kim, Sae Yun Kwon, Spencer J. Washburn, Scott C. Brooks, Ji Won Yoon, Lucien Besnard
Summary: The study uses Hg isotope ratios to identify the sources and exposure pathways of mercury in bivalves, finding that dissolved Hg phases in the water column are the primary source and exposure pathway to bivalves. This provides new insights into using bivalves as bioindicators for sediment quality monitoring.
Article
Engineering, Environmental
Hui Geng, Ying Xu, Rui Liu, Dianhai Yang, Xiaohu Dai
Summary: This study investigates the effect of cation exchange resin (CER) on the sequential recovery of hydrogen and methane from anaerobic digestion (AD) and the corresponding mechanisms. The results show that CER can simultaneously enhance the production of hydrogen and methane by promoting the solubilisation, hydrolysis, and acidification of organic matter. Additionally, CER facilitates effective contact between bacteria and organic particulates and reduces the energy barrier for mass transfer during methane production. The study also reveals changes in the microbial community structure and metagenomics during the AD process.
Article
Engineering, Environmental
Xiaojing Lin, Zhan Jin, Shunfeng Jiang, Zhiquan Wang, Suqing Wu, Ke Bei, Min Zhao, Xiangyong Zheng
Summary: Dehumidification combined with addition of absorbent resin supplement (ARS) was used to achieve rapid evaporation of non-pretreated urine, resulting in high water evaporation efficiency and nutrient recovery.
Article
Engineering, Environmental
Yangli Che, Chaoran Lin, Shen Li, Jiao Liu, Longhai Zhu, Shilei Yu, Nan Wang, Haoshuai Li, Mutai Bao, Yang Zhou, Tonghao Si, Rui Bao
Summary: Hydrodynamic processes play a crucial role in the transmission of sediments, microbial assembly, and organic carbon redistribution in the ocean. Through experiments and analysis, we found that hydrodynamics shape the assembly of microbial communities and control the redistribution of different sourced organic carbon, thereby influencing microbial-mediated biogeochemical transformation.
Article
Engineering, Environmental
Chao Chen, Yu Yang, Nigel J. D. Graham, Zhenyu Li, Xingtao Yang, Zhining Wang, Nadia Farhat, Johannes S. Vrouwenvelder, Li -an Hou
Summary: The fouling of seawater reverse osmosis membranes is a persistent challenge in desalination. This study monitored the operational performance of a desalination plant for 7 years and the fouling development in different areas of membrane modules. The findings showed that operational performance declined over time and fouling mainly occurred at the feed side of the modules, with the highest microbial diversity. Keystone species like Chloroflexi and Planctomycetes played an important role in maintaining community structure and biofilm maturation. Polysaccharides, soluble microbial products, marine humic acid-like substances, and inorganic substances contributed to fouling. Overall, biofouling had a significant impact on membrane fouling after 7 years of operation.
Article
Engineering, Environmental
Dan Li, Jieyi Sun, Yibo Fu, Wentao Hong, Heli Wang, Qian Yang, Junhong Wu, Sen Yang, Jianhui Xu, Yunfei Zhang, Yirong Deng, Yin Zhong, Ping'an Peng
Summary: Sulfidation-oxidation treatment of magnetite (Fe3O4) enhances the production of dark center dot OH, which can efficiently degrade dissolved organic matter (DOM) and accelerate carbon cycling.
Article
Engineering, Environmental
Cheng Yu, Kaijun Wang, Kaiyuan Zhang, Ruiyang Liu, Pingping Zheng
Summary: This study implemented a microaerobic-aerobic configuration in a full-scale municipal wastewater treatment facility and investigated the effects on sludge characteristics, pollutant removal, microbial community, and granulation mechanisms. The results showed successful transition from flocculent-activated sludge to well-defined AGS after two months of operation. The primary pathways for pollutant removal were simultaneous nitrification, denitrification, and phosphorus removal. Moreover, the incorporation of internal separators induced shifts in the flow pattern, which promoted granulation.
Article
Engineering, Environmental
Zhe Zhang, Shaoyang Hu, Guangrong Sun, Wei Wang
Summary: Halogenated aromatic disinfection byproducts (DBPs), such as halogenated phenols, have garnered widespread attention due to their high toxicity and prevalence. This study reports on the analysis, occurrence, and cytotoxicity of a group of emerging halogenated aromatic DBPs, known as halogenated polyhydroxyphenols (HPPs), in drinking water.
Article
Engineering, Environmental
Shengyue Chen, Jinliang Huang, Peng Wang, Xi Tang, Zhenyu Zhang
Summary: Accurate prediction of river water quality is crucial for sustainable water management. This study introduces wavelet analysis and transfer learning techniques to assist LSTM modeling, proposing a newly coupled modeling approach that improves short-term prediction of river water quality.
Article
Engineering, Environmental
Bang Du, Xinmin Zhan, Piet N. L. Lens, Yifeng Zhang, Guangxue Wu
Summary: Efficient anaerobic digestion relies on the cooperation of different microorganisms with different metabolic pathways. This study investigated the effects of different operational modes and the addition of powdered activated carbon (PAC) on ethanol metabolic pathways. The results showed that the SBR mode and the presence of CO2 facilitated ethanol metabolism towards propionate production, while the CFR mode with extended solids retention time enriched Geobacter. Adjusting operational modes and PAC addition can modulate anaerobic ethanol metabolism and enrich Geobacter.
Article
Engineering, Environmental
Wanfa Wang, Si-Liang Li, Jun Zhong, Yuanbi Yi, Fujun Yue, Zenglei Han, Qixin Wu, Ding He, Cong-Qiang Liu
Summary: This study compares the carbon biogeochemical processes in karst and non-karst regions within large thermal stratified river-reservoir systems. The results demonstrate that karst reservoirs have a reduced potential for carbon emissions and highlight the importance of considering geologic settings to improve accuracy in regional and global CO2 emission estimates.
Article
Engineering, Environmental
Chunxia Jiang, Zelong Zhao, Dong Zhu, Xiong Pan, Yuyi Yang
Summary: This study analyzed the occurrence and distribution of antibiotic resistance genes (ARGs) in different environmental media of the Yangtze River using metagenomics. Core resistome dominated by multidrug resistance genes was found in all samples, while rare resistome dominated by various resistance genes was more prevalent in plasmids. Specific bacteria were identified as hosts for both core and rare resistomes, with high clinical concern ARGs found in the rare resistome. Particle-associated environment provided the most ideal conditions for resistome hosts. This study provided insights into the genetic locations of ARGs and the community assembly mechanisms of ARG hosts in freshwater environments.
Article
Engineering, Environmental
Yu Zhang, Yongtao He, Linchun Jia, Lei Xu, Zheng Wang, Yueling He, Ling Xiong, Xumeng Lin, Hong Chen, Gang Xue
Summary: By synergizing organic carbon source, thiosulfate, and zero-valent iron, efficient mixotrophic denitrification of oligotrophic secondary effluent can be achieved. Thiosulfate plays a vital role in promoting TN removal efficiency, while corrosion of Fe0 releases OH- to neutralize H+ from thiosulfate-driven denitrification, creating a suitable environment for denitrification. The coordination of thiosulfate and Fe0 maintains the dominance of Thiobacillus for denitrification.
