期刊
CURRENT BIOLOGY
卷 26, 期 17, 页码 2364-2369出版社
CELL PRESS
DOI: 10.1016/j.cub.2016.06.064
关键词
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资金
- Czech Science Foundation [13-24983S, 16-18699S]
- National Feasibility Programme I of the Czech Republic [LO1208]
- Statutory City of Ostrava [0924/2016/SaS]
A limited number of non-canonical genetic codes have been described in eukaryotic nuclear genomes. Most involve reassignment of one or two termination codons as sense ones [1-4], but no code variant is known that would have reassigned all three termination codons. Here, we describe such a variant that we discovered in a Glade of trypanosomatids comprising nominal Blastocrithidia species. In these protists, UGA has been reassigned to encode tryptophan, while UAG and UAA (UAR) have become glutamate encoding. Strikingly, UAA and, less frequently, UAG also serve as bona fide termination codons. The release factor eRF1 in Blastocrithidia contains a substitution of a conserved serine residue predicted to decrease its affinity to UGA, which explains why this triplet can be read as a sense codon. However, the molecular basis for the dual interpretation of UAR codons remains elusive. Our findings expand the limits of comprehension of one of the fundamental processes in molecular biology.
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