期刊
CURRENT BIOLOGY
卷 26, 期 12, 页码 1532-1542出版社
CELL PRESS
DOI: 10.1016/j.cub.2016.04.067
关键词
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资金
- MEXT [25430001, 15K14306, 26113702, 26114502, 23220007, 25132702, 24700309]
- Takeda Science Foundation
- Grants-in-Aid for Scientific Research [25430001, 26113702, 24700309, 16H06371, 23220007, 15K14306, 26114502] Funding Source: KAKEN
The Drosophila fruitless (fru) gene is regarded as a master regulator of the formation of male courtship circuitry, yet little is known about its molecular basis of action. We show that roundabout 1 (robot) knockdown in females promotes formation of the male-specific neurite in sexually dimorphic mAL interneurons and that overexpression of the male-specific Fre(BM) diminishes the expression of Robo1 in the fly brain. Our electrophoretic mobility shift and reporter assays identify the 42-bp segment encompassing the palindrome sequence T T C G C T G C GCCGTGAA in the 5' UTR of robo1 exon1 as the Fru(BM)-responsive element. We find that similar to 10-bp deletions in the palindrome sequence induce a loss of the male-specific neurite and disrupt male courtship patterns. This study paves the way for a thorough understanding of the mechanism whereby Fru proteins orchestrate transcription for the formation of courtship circuitry.
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