Review
Cell & Tissue Engineering
Liangrui Chen, Shasha Zhu, Shujuan Guo, Weidong Tian
Summary: Periodontitis is a common oral disease that causes damage to both hard and soft tissues of the periodontium, leading to tooth mobility and loss. Mesenchymal stem cells (MSCs) have emerged as a promising therapy for periodontitis due to their unique characteristics. Accumulating evidence suggests that the regenerative effects of MSCs are mainly mediated through paracrine signaling, including the release of extracellular vesicles (EVs) that contain bioactive substances. These MSC-EVs play a crucial role in regulating the biological functions of periodontal tissue cells, immune cells, and the local microenvironment, promoting tissue regeneration and repair. As a cell-free therapy, MSC-EVs offer advantages in terms of stability, immune rejection, and ethical concerns, making them potentially valuable for clinical applications in periodontal regeneration.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Biotechnology & Applied Microbiology
Dao-Kun Deng, Jiu-Jiu Zhang, Dian Gan, Jie-Kang Zou, Rui-Xin Wu, Yi Tian, Yuan Yin, Xuan Li, Fa-Ming Chen, Xiao-Tao He
Summary: This review highlights the roles of extracellular vesicles (EVs) in periodontal homeostasis and their potential in periodontal regenerative strategies. The pathogenic roles of bacteria-derived EVs (BEVs) in plaque formation, immune evasion, inflammatory pathway activation, and tissue destruction are discussed, along with the multifunctional biological effects of cell-derived EVs (CEVs) on periodontal tissue regeneration.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Leila Gholami, Vajihe Taghdiri Nooshabadi, Shiva Shahabi, Marzieh Jazayeri, Rana Tarzemany, Zohreh Afsartala, Khatereh Khorsandi
Summary: Oral mesenchymal stem cells (MSCs) and their secretomes play a crucial role in medical tissue engineering and cell free biotherapy due to their accessibility and therapeutic outcomes. Recent studies have focused on the regenerative ability of EVs and CM from MSCs in bone and periodontal defects.
CELL AND BIOSCIENCE
(2021)
Review
Polymer Science
Maoxue Li, Jiaxi Lv, Yi Yang, Guoping Cheng, Shujuan Guo, Chengcheng Liu, Yi Ding
Summary: Hydrogel, a functional polymer material, has high biocompatibility and slow release properties. It provides suitable attachment sites and growth environments for periodontal cells, playing a significant role in periodontal tissue engineering.
Article
Materials Science, Multidisciplinary
Yuyang Li, Haoran Peng, Wenyue Tang, Deao Gu, Shuangshuang Ren, Yijun Yu, Jie Yang, Leiying Miao
Summary: An injectable hydrogel loaded with ceria nanoparticle (CNP) and erythropoietin (EPO) was prepared for the treatment of irregular periodontal pocket. The composite hydrogel showed the potential to reduce inflammation and promote periodontal regeneration, making it a promising candidate for periodontitis treatment.
MATERIALS & DESIGN
(2023)
Article
Engineering, Biomedical
Jana Zarubova, Mohammad Mahdi Hasani-Sadrabadi, Erfan Dashtimoghadam, Xuexiang Zhang, Sahar Ansari, Song Li, Alireza Moshaverinia
Summary: A delivery platform for small extracellular vesicles derived from gingival mesenchymal stem cells (GMSCs) has been developed to treat periodontitis, showing potential clinical applications in promoting regeneration of damaged periodontal tissues.
ADVANCED HEALTHCARE MATERIALS
(2022)
Review
Pharmacology & Pharmacy
Huiling Deng, Jiecong Wang, Ran An
Summary: This article summarizes the important role of exosomes in bone regeneration and the problems in their clinical application. It then introduces the use of hydrogels as a solution to these problems, with a focus on the application prospects and challenges of hyaluronic acid-based hydrogels as exosome delivery devices in bone regeneration.
FRONTIERS IN PHARMACOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Xinyu Song, Ling Xu, Wenjie Zhang
Summary: Critical-size bone defect repair is challenging, and current therapies have limitations. Extracellular vesicle (EV)-based strategies offer a promising approach for tissue regeneration due to their complex composition and low immunogenicity. Optimizing the synthesis of biomimetic EVs can enhance their bioactivity and improve bone regeneration. Integrating nanoparticles into bone graft biomaterials can maximize the therapeutic efficacy of EVs. This review summarizes the role of EVs in bone regeneration, discusses strategies for optimizing biomimetic synthetic EVs, and explores the use of bone graft biomaterials for EV delivery to repair bone defects.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Nanoscience & Nanotechnology
Cheng Hu, Mei Zhang, Jin Wu, Xing Cao, Luxian Chen, Jiayin Yan, Gaolin Liang, Jiali Tan
Summary: A novel biomaterial was synthesized to promote periodontal tissue regeneration by inhibiting osteoclast overactivation. It displayed outstanding mechanical properties and 3D structure, and could inhibit the proliferation of macrophages and differentiation of osteoclasts. This study provides a new strategy for tissue regeneration engineering.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Engineering, Biomedical
Pengzhen Cheng, Tianqing Cao, Xueyi Zhao, Weiguang Lu, Sheng Miao, Fenru Ning, Dong Wang, Yi Gao, Long Wang, Guoxian Pei, Liu Yang
Summary: This study identifies the intracellular binding target of EV-NID1 and further elucidates a novel approach and mechanism, thereby providing a cell-free construction strategy with precise targets for the development of vascularized tissue engineering products.
BIOACTIVE MATERIALS
(2022)
Article
Engineering, Biomedical
Pingping Yuan, Zhiye Li, Bo Shao, Tian Zeng, Xiaopeng Wu, Yinggang Wang, Yimin Zhao, Wei Wu
Summary: In this study, BMSCs pre-conditioned in a starving environment were found to enhance the anti-apoptosis potential of co-transplanted chondrocytes, and extracellular vesicles derived from starving BMSCs played essential roles in improving apoptosis and regulating autophagy of chondrocytes. In vivo studies confirmed that these EVs significantly improved the survival of chondrocyte bricks.
ACTA BIOMATERIALIA
(2022)
Review
Biochemistry & Molecular Biology
Giusi Alberti, Eleonora Russo, Simona Corrao, Rita Anzalone, Peter Kruzliak, Vitale Miceli, Pier Giulio Conaldi, Francesca Di Gaudio, Giampiero La Rocca
Summary: Extracellular vesicles (EVs) are one of the main mechanisms of communication between cells and the surrounding tissue or at distance. Recent studies have revealed the molecular composition and clinical indications of EVs derived from adult mesenchymal stromal cells (MSCs). These EVs maintain some of the properties of their parent cells and have important implications for cell-free therapies.
Article
Biotechnology & Applied Microbiology
Wuzheng Xia, Guanzhi Lai, Yichuan Li, Cong Zeng, Chengjun Sun, Pinzhe Zhang, Guanghao Zhu, Leping Li, Linwei Wu
Summary: This study aims to develop a composite hydrogel dressing with excellent hemostatic properties, biocompatibility, and ability to promote liver cell regeneration for the treatment of liver diseases. GelMA/Alg-DA-1 composite hydrogel showed the best physical and chemical properties, good biocompatibility, and hemostatic function. Furthermore, loading adipose-derived mesenchymal stem cell exosomes (AD-MSC-Exo) into GelMA/Alg-DA-1 hydrogel can effectively promote cell proliferation and migration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Serhat Aslan, Nurcan Buduneli, Pierpaolo Cortellini
Summary: This study evaluated the clinical applicability of the entire papilla preservation technique in the regenerative treatment of deep intrabony defects, showing promising results in providing optimal biomaterial protection and healing conditions.
JOURNAL OF PERIODONTOLOGY
(2021)
Review
Polymer Science
Gamal Abdel Nasser Atia, Hany K. Shalaby, Mehrukh Zehravi, Mohamed Mohamady Ghobashy, Hager Abdel Nasser Attia, Zubair Ahmad, Farhat S. Khan, Abhijit Dey, Nobendu Mukerjee, Athanasios Alexiou, Md. Habibur Rahman, Joanna Klepacka, Agnieszka Najda
Summary: This article examined and summarized the current developments in chitosan-based materials, focusing on the modification of chitosan and its use in periodontal regeneration. The study critically reviewed various preparation and modification techniques to enhance chitosan performance and discussed the uses of chitosan-based products in depth.
Article
Cell & Tissue Engineering
Xun Xu, Li Liao, Weidong Tian
Summary: Prevascularization is an effective strategy to promote the survival and regeneration of grafts after transplantation, especially in repairing craniofacial tissue defects. The repair of craniofacial tissue defects is critical and transplantation of engineered tissues or biomaterials shows promising prospects. Revascularization plays a crucial role in ensuring the success of tissue regeneration.
TISSUE ENGINEERING PART B-REVIEWS
(2022)
Review
Dentistry, Oral Surgery & Medicine
Gang Liu, Xiaoning Qiu, Xinzhi Tan, Rong Miao, Weidong Tian, Wei Jing
Summary: This study assessed the efficacy of topical sialogogue spray containing 1% malic acid for treating xerostomia. The results showed that this spray significantly improved dry mouth symptoms and increased saliva flow rates. However, due to the limited number of studies, more research is needed in the future to provide higher quality evidence.
Article
Nanoscience & Nanotechnology
Min He, Qian Wang, Yunbo Feng, Xinghui Gao, Chao He, Jiangge Li, Weifeng Zhao, Weidong Tian, Changsheng Zhao
Summary: This study proposes an amphiphilic nanomedicine with dual anti-inflammatory functions and inflammation-responsive drug release properties to spatiotemporally manage the osteoimmunomodulation of the bone scaffold, potentially improving in vivo osteogenesis.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Engineering, Biomedical
Xi Liang, Li Xie, Qingyuan Zhang, Ge Wang, Siyuan Zhang, Mingyan Jiang, Ruitao Zhang, Ting Yang, Xingyu Hu, Ziyang Yang, Weidong Tian
Summary: This study successfully fabricated GelMA-alginate core-shell microcapsules using a novel preparation technique, and co-cultured human dental pulp stem cells (hDPSCs) and human umbilical vein endothelial cells (HUVECs) in them. In the microcapsules, the co-culture group showed higher cell proliferation rates and the formation of 3D capillary-like networks. Furthermore, the co-culture group exhibited enhanced osteo/odontogenic differentiation. In vivo experiments confirmed better microvessel formation and pulp-like tissue regeneration in the co-culture group.
ACTA BIOMATERIALIA
(2022)
Article
Computer Science, Artificial Intelligence
Lei Xu, Shanluo Zhou, Jixiang Guo, Weidong Tian, Wei Tang, Zhang Yi
Summary: Metal artifacts in oral and maxillofacial CT images can affect imaging quality and pose a threat to patients' lives. This study proposes and validates a generative adversarial network (GAN) based model, MAR-GAN, to reduce these artifacts and improve image quality. The effectiveness of MAR-GAN is demonstrated on both simulated and clinical datasets, showing superior performance and clinically acceptable results.
APPLIED INTELLIGENCE
(2022)
Article
Engineering, Environmental
Zhaoyu Xu, Xingyu Hu, Li Xie, Ruojing Qian, Suru Liu, Xinzhi Tan, Meiying Shao, Chuan He, Kui Ma, Jie Long, Weidong Tian
Summary: This study proposes a safe and efficient novel root canal irrigant system with good antibacterial and tissue dissolution activities. The system exhibited excellent antibacterial effects against common clinical bacterial strains and biofilms, as well as desirable pulp tissue-dissolving efficacy.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Engineering, Multidisciplinary
He Min, Kaide Li, Qian Wang, Xinghui Gao, Li Xie, Weidong Tian
Summary: Tannic acid (TA)/Zn2+ based metal-phenolic network complexes (MPNs) were assembled as a novel filler for scaffolds, providing immunomodulatory and antibacterial functions. The sustained release of components from the filler controlled inflammation and offered long-term antibacterial performance. This study paved a new path for the application of MPNs in multi-functional biocomposites.
COMPOSITES PART B-ENGINEERING
(2022)
Article
Dentistry, Oral Surgery & Medicine
Yanli Huang, Li Liu, Qian Liu, Fangjun Huo, Xingyu Hu, Shujuan Guo, Weidong Tian
Summary: The study demonstrates that D-sEV and L-D-sEV can inhibit the pathogenicity of P. gingivalis and prevent the development of periodontitis.
Article
Cell & Tissue Engineering
Chuan He, Minjia Dai, Xiaojie Zhou, Jie Long, Weidong Tian, Mei Yu
Summary: Our study compared adipose tissue extract (ATE) and its further purified small extracellular vesicles (sEV-AT) in terms of characteristics, inductivity on cells, and capacity for promoting de novo adipose tissue regeneration. sEV-AT showed higher particle yield and concentrated particle size distribution, while ATE had more soluble factors and multiple peaks in particle size distribution.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Biology
Zhi Liu, Tian Chen, Sicheng Zhang, Tianfang Yang, Yun Gong, Hong-Wen Deng, Ding Bai, Weidong Tian, YiPing Chen
Summary: This study identified a population of adipocytes, named Wnt(+) adipocytes, that exhibit persistent activity of the Wnt/β-catenin signaling pathway. The activation of this pathway is independent of Wnt ligands and receptors but relies on the AKT/mTOR pathway and is crucial for cell survival. These adipocytes have distinct transcriptomic and genomic signatures from classical adipocytes and can be induced from various sources of mesenchymal stromal cells. Furthermore, they convert into beige adipocytes directly and are required for beige fat recruitment under thermal challenge.
Article
Biotechnology & Applied Microbiology
Yan Zhang, Mei Yu, Jia Dong, Yue Wu, Weidong Tian
Summary: This study revealed that NPM3 derived from brown adipose tissue can induce browning of white adipose tissue by regulating the stability of PRDM16 mRNA. Supplementation of sEVs-BAT may represent a promising therapeutic strategy for promoting thermogenesis and energy expenditure, particularly in obesity.
JOURNAL OF NANOBIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Ziyang Yang, Li Xie, Boqing Zhang, Gang Zhang, Fangjun Huo, Changchun Zhou, Xi Liang, Yujiang Fan, Weidong Tian, Yinghui Tan
Summary: This study demonstrates the potential of the BMP-2/PDA-BCP scaffold for the treatment of small craniofacial bone defects requiring high accuracy. The scaffold, constructed using DLP-based 3D printing, showed excellent properties and effectively promoted bone regeneration both in vitro and in vivo.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Qian Liu, Shujuan Guo, Yanli Huang, Xiuqun Wei, Li Liu, Fangjun Huo, Ping Huang, Yafei Wu, Weidong Tian
Summary: Objective of this study was to explore the role and therapeutic function of TRPA1 in periodontitis. The study found that TRPA1 inhibitor significantly reduced oxidative and apoptotic levels in inflammatory cells, as well as reduced oxidative stress and apoptosis in periodontitis mice.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Review
Dentistry, Oral Surgery & Medicine
Ting Yang, Li Xie, Rui Tao Zhang, Wei Dong Tian
Summary: Microspheres, as a new type of injectable scaffold, have attracted increasing attention in the field of regenerative medicine. Their small size, large specific surface area, and mimicry of the 3D native environment make them ideal for endodontic regeneration. They play an important role in carrying biologics and controlling the fate of stem cells as well as the release of growth factors and drugs.
CHINESE JOURNAL OF DENTAL RESEARCH
(2022)
Article
Materials Science, Biomaterials
Gang Liu, Boqing Zhang, Ting Wan, Changchun Zhou, Yujiang Fan, Weidong Tian, Wei Jing
Summary: In this study, a novel bioactive scaffold loaded with platelet lysate/gelatin methacrylate (PL/GelMA) in a 3D-printed biphasic calcium phosphate (BCP) scaffold was proposed to enhance scaffold vascularization. The PL/GelMA/BCP scaffold successfully promoted cell proliferation and the formation of blood vessel-like structures in vitro. Moreover, implantation of the scaffold in vivo also resulted in increased capillary formation. These findings suggest that the PL/GelMA/BCP scaffold has the potential to promote vascularization in tissue engineering.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
