Article
Microbiology
Li Wang, Junyu Chen, Lin He, Hanbo Liu, Yan Liu, Zonghui Luan, Hong Li, Weixin Liu, Mengjun Luo
Summary: The aim of this study was to investigate the distribution and differences in the uterus and vaginal microbiome between women with early embryonic arrest and those with normal pregnancy using high-throughput sequencing. The results showed significant differences in Lactobacillus, Bacteroidetes, and Helicobacter in the vagina between the two groups. The study also identified specific dominant bacterial species and genera in both the vagina and uterus.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Li-Ge Bu, Bo Wang, Ting-Yue Li, Ya Sun, Li-Li Kong, Zhen-Ao Zhao, Shi-Jie Li, Nai-Zheng Ding, Hua Ni
Summary: This study found that FOXO1 plays a key role in conceptus adhesion in goats during early pregnancy, and it modulates the synthesis of prostaglandins by regulating the transcription of PTGS2. IFNT upregulates the levels of FOXO1 and PTGS2. These findings contribute to a better understanding of the function of FOXO1 in the reproductive implantation of small ruminants.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Endocrinology & Metabolism
Xiaolong Du, Yufang Liu, Xiaoyun He, Lin Tao, Meiying Fang, Mingxing Chu
Summary: In this study, the uterus of Yunshang black goats with high and low fecundity in the proliferative period was analyzed using RNA sequencing and bioinformatics analysis. The study identified differentially expressed mRNAs, lncRNAs, and miRNAs, as well as predicted their target genes and constructed interaction networks. The results provide valuable information for understanding the mechanisms associated with high fecundity in goats and reducing pregnancy loss.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Adomar Laurindo Neto, Felipe Lopes Ruas, Bruno Silva Espirito Santo, Ana Clara Degan Mattos, Junior Borges Silva, Marcos Flavio Diniz Jr, Danilo Fonseca Cardoso Pereira, Izabelle Pereira Lacerda, Jose Oliveira Carvalho, Guilherme Pugliesi
Summary: This study tested the impact of supplementing long-acting P4 at different times during the initial dioestrus on the pregnancy rates of dairy and beef recipients undergoing fixed-time embryo transfer. The results showed that using iP4 during FTET can enhance the pregnancy rates of beef recipients, although the effectiveness of iP4 supplementation on pregnancy maintenance may vary under different experimental conditions.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Reproductive Biology
Ya Sun, Xin-Cheng Zhang, Meng-Die Li, Li-Ge Bu, Bo Wang, Ting-Yue Li, Nai-Zheng Ding, Hua Ni
Summary: N-6-methyladenosine (m6A), an epigenetic modification, increases in goat uterus during early pregnancy and is regulated by METTL3. METTL3 positively regulates CTGF expression, which affects the proliferation of endometrial epithelial cells (EECs). These findings provide a foundation for further understanding the crucial role of METTL3-mediated m6A modification in goat uterus during early pregnancy.
BIOLOGY OF REPRODUCTION
(2023)
Article
Agriculture, Dairy & Animal Science
Ya Sun, Li-Ge Bu, Bo Wang, Jie Ren, Ting-Yue Li, Li-Li Kong, Hua Ni
Summary: Pregnancy success is closely related to the regulation of energy metabolism balance, and this study reveals the expression and hormone regulation of uncoupling protein 2 (UCP2) in goat uterus. UCP2 is dynamically expressed in the uterus during early pregnancy and can affect the proliferation of endometrial epithelial cells, suggesting its involvement in regulating energy metabolism balance.
ANIMAL REPRODUCTION SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Kaibin Fu, Xiang Chen, Wei Guo, Zhinan Zhou, Yan Zhang, Taotao Ji, Peifang Yang, Xingzhou Tian, Weiwei Wang, Yue Zou
Summary: NAC can alter the expression of uterine genes in goats in early gestation, and these differentially expressed genes may regulate uterine performance in early pregnancy by participating in signalling pathways related to reproductive regulation, resistance to oxidative stress, immune regulation, angiogenesis and development, cytokines, and cell adhesion. These findings provide important references for the modulation of reproductive performance in goats during early gestation by NAC.
Article
Biochemistry & Molecular Biology
Xiao Ma, Jing Wang, Likai Wang, Laiqing Yan, Yunjie Liu, Wenkui Ma, Pengyun Ji, Lu Zhang, Guoshi Liu
Summary: Using Aanat and Mt2 KO mice, this study found that the melatonergic system plays a crucial role in early pregnancy in mice. The deficiency of AANAT and MT2 resulted in reduced implantation sites and abnormal endometrium morphology. Mechanistically, the melatonergic system is essential for normal endometrial receptivity and functions by activating the STAT signaling pathway. Melatonin production reduction and signal transduction impairment led to hyperproliferative endometrium and increased local immunoinflammatory reaction, causing early abortion in Mt2 KO mice. These findings might have implications in other animals, including humans.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Reproductive Biology
Jirapat Jaisue, Takahiro Nii, Naoki Suzuki, Yusaku Tsugami, Naoki Isobe
Summary: Repeated intrauterine infusion of lipopolysaccharide (LPS) can induce more severe inflammation in goat mammary glands. The inflammation is characterized by increased somatic cell count, higher levels of plasma LPS-binding protein, and elevated concentrations of pro-inflammatory cytokines interleukin-1β, tumor necrosis factor-α, and interleukin-8.
Article
Veterinary Sciences
Daniela E. Barraza, Luciana M. Sari, Silvana A. Apichela, Marcelo H. Ratto, Martin E. Arganaraz
Summary: The majority of pregnancies in South American camelids are established in the left uterine horn (LUH) and may be related to differential vascular irrigation. This study found that beta-NGF and TrKA expression was stronger in LUH of early pregnant alpacas, indicating their involvement in implantation and pregnancy development.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Veterinary Sciences
Katarzyna Buska-Mach, Anna Ewa Kedzierska, Adam Lepczynski, Agnieszka Herosimczyk, Malgorzata Ozgo, Pawel Karpinski, Agnieszka Gomulkiewicz, Daria Lorek, Anna Slawek, Piotr Dziegiel, Anna Chelmonska-Soyta
Summary: This study demonstrated that pregnant female mice can distinguish between embryos of normal and impaired biological quality before implantation, showing differential gene expression in signal transduction pathways and differential protein expression in splenic CD4(+) T lymphocytes.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Obstetrics & Gynecology
Wei Xiong, Xin Tan, Yana Liu, Jie Liu, Xue Dong, Zhilin Wang, Hengxi Chen
Summary: This study compared the clinical outcomes and second-look hysteroscopy findings in patients with complete and incomplete septate uteri after septoplasty. The results showed similar pregnancy outcomes and no significant difference in intrauterine adhesion rates between the two groups. Endometrial polyps were common in septate uteri.
ARCHIVES OF GYNECOLOGY AND OBSTETRICS
(2023)
Article
Environmental Sciences
Yan Zhang, Linchao Zhang, Jialu Bao, Liantao Liu, Xiaodan Wang
Summary: The study revealed that PFOA can increase liver weight, reduce uterine index, and induce toxic effects on liver and uterine tissues. Additionally, various factors expressed in uterine cells showed dose-dependent changes in response to PFOA exposure.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Reproductive Biology
Nirvay Sah, Claire Stenhouse, Katherine M. Halloran, Robyn M. Moses, Heewon Seo, Robert C. Burghardt, Gregory A. Johnson, Guoyao Wu, Fuller W. Bazer
Summary: In this study, the metabolism of creatine (Cr) at the uterine-conceptus interface in sheep during early gestation was investigated. The results showed an increase in the levels of Cr and guanidinoacetate (GA) between Days 12 and 17 of pregnancy. The expression of mRNAs related to GA formation, Cr synthesis, and Cr/PCr utilization was higher on Days 17 and 21 compared to earlier days of pregnancy. These findings provide insights into the specific metabolism of Cr at the uterine-conceptus interface and suggest a role for the Cr-CK-PCr system in ovine conceptus development and implantation.
BIOLOGY OF REPRODUCTION
(2022)
Article
Agriculture, Dairy & Animal Science
Peifang Yang, Xiang Chen, Xingzhou Tian, Zhinan Zhou, Yan Zhang, Wen Tang, Kaibin Fu, Jiafu Zhao, Yong Ruan
Summary: This study investigated the effect of N-acetyl-L-cysteine (NAC) supplementation on the expression of uterine keratin gene and protein in goats during early pregnancy. The results showed significant differences in uterine keratin expression and increases in antioxidant indices and hormone levels in the serum of goats. These findings provide new insight into the mechanism by which NAC regulates early pregnancy and embryonic development in goats.
Article
Reproductive Biology
Li-Ge Bu, Ya Sun, Ting-Yue Li, Li-Li Kong, Hao-Nan Yu, Shi-Jie Li, Nai-Zheng Ding, Hua Ni
Summary: In this study, the expression and regulation of ITGB8 in goat uterus were investigated, and the miR-187/ITGB8 axis was found to be potentially involved in conceptus attachment in response to interferon tau.
Article
Agriculture, Dairy & Animal Science
Ya Sun, Li-Ge Bu, Bo Wang, Jie Ren, Ting-Yue Li, Li-Li Kong, Hua Ni
Summary: Pregnancy success is closely related to the regulation of energy metabolism balance, and this study reveals the expression and hormone regulation of uncoupling protein 2 (UCP2) in goat uterus. UCP2 is dynamically expressed in the uterus during early pregnancy and can affect the proliferation of endometrial epithelial cells, suggesting its involvement in regulating energy metabolism balance.
ANIMAL REPRODUCTION SCIENCE
(2022)
Article
Reproductive Biology
Ya Sun, Xin-Cheng Zhang, Meng-Die Li, Li-Ge Bu, Bo Wang, Ting-Yue Li, Nai-Zheng Ding, Hua Ni
Summary: N-6-methyladenosine (m6A), an epigenetic modification, increases in goat uterus during early pregnancy and is regulated by METTL3. METTL3 positively regulates CTGF expression, which affects the proliferation of endometrial epithelial cells (EECs). These findings provide a foundation for further understanding the crucial role of METTL3-mediated m6A modification in goat uterus during early pregnancy.
BIOLOGY OF REPRODUCTION
(2023)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.