Article
Chemistry, Analytical
Sha Li, Fei-Long Liu, Zheng Zhang, Xiao-Ming Yin, Tian-Tian Ye, Bi-Feng Yuan, Yu-Qi Feng
Summary: In this study, a novel stable isotope chemical labeling combined with reversed-phase LC-MS method was developed for ultrasensitive determination of sugar phosphates at the single-cell level. The method allowed for improved separation and identification of sugar phosphate isomers, resulting in increased detection sensitivities and accurate quantification of 12 different sugar phosphates in trace biological samples.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Brittany Widner, Melissa C. Kido Soule, Frank Xavier Ferrer-Gonzalez, Mary Ann Moran, Elizabeth B. Kujawinski
Summary: Utilizing pre-extraction derivatization and ultrahigh performance liquid chromatography electrospray ionization tandem mass spectrometry, targeted dissolved metabolites in seawater and saline culture media were successfully detected and quantified. This quantitative method has been optimized for 73 common metabolites, with 50 requiring derivatization for quantification due to low extraction efficiencies.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
L. Perez-Mayan, G. Castro, M. Ramil, R. Cela, I Rodriguez
Summary: Two different analytical methodologies were evaluated for detecting GLY and AMPA residues in wine samples, with one utilizing derivative transformation and the other direct determination. The use of molecularly imprinted sorbent for extraction and a strong anionic exchange LC column for direct determination proved to be effective in overcoming the challenges presented by the wine matrix.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
V. Fern, J. M. Andrade-Garda, S. Muniategui-Lorenzo
Summary: The low percentages of PVC reported in academic studies may be due to various factors, highlighting the need for suitable spectroscopic databases that include PVC weathered standards.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Engineering, Environmental
Wen Ma, Jun Li, Xianjiang Li, Huwei Liu
Summary: A series of metal-organic frameworks were synthesized and used as adsorbents for five diamide insecticides, with MIL-101-NH2 showing superior performance. A sensitive method combining dispersive solid phase extraction with liquid chromatography tandem mass spectrometry was developed, achieving high enrichment efficiency by investigating key parameters. The proposed method demonstrated ultrahigh sensitivity and wide linearity for the analysis of diamide insecticides.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Chemistry, Analytical
Sansi Xing, Akshat Pai, Ruilin Wu, Yu Lu
Summary: A novel hybrid-isotope labeling approach named NETLOP was presented in this work, which allows for higher sample multiplexity and was demonstrated to be feasible and effective in proteomic analysis.
ANALYTICAL CHEMISTRY
(2021)
Review
Chemistry, Analytical
Yuelin Song, Qingqing Song, Wenjing Liu, Jun Li, Pengfei Tu
Summary: Currently, MS/MS is widely used in metabolomics for the identification of metabolites. However, the structural identification of isomers remains challenging due to the similarity of their MS/MS spectra. Differentiation and identification of isomers using MS/MS can be facilitated by various techniques, including chemical derivatization, ion mobility spectrometry, ion dissociation techniques, and energy-resolved MS. This tutorial provides a comprehensive summary of these cutting-edge techniques and emphasizes the importance of isomeric identification in metabolomics for analytical chemists. (c) 2023 Elsevier B.V. All rights reserved.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Organic
Neng Wang, Hang Wang, Lin-Xi Wan, Xiao-Huan Li, Xian-Li Zhou, Jia-Hong Li, Steven De Jonghe, Dominique Schols, Jin-Bu Xu, Feng Gao
Summary: The biorelevant sulfur-containing Euphorbia diterpenes with scarce 5/7/6/3 premyrsinane-and 5/7/6 myrsinanetype backbones were easily synthesized from naturally abundant lathyrane-type Euphorbia factor L3 by visible-light-triggered tandem thiol-ene click reaction/transannular cyclization and regioselective cyclopropane ring-opening. The universality of this synthesis route was confirmed by successfully obtaining the selenide diterpene. This concise synthesis strategy offers a mild and efficient method for obtaining structurally diverse Euphorbia diterpenes, including a promising anti-HIV bioactive premyrsinane diterpene 3h.
Article
Biochemistry & Molecular Biology
Tong Li, Ruiguo Wang, Peilong Wang
Summary: Biogenic amines (BAs) are formed from amino acids and can have various structures. In our study, a method using ultra-performance liquid chromatography-tandem mass spectrometry was developed to rapidly determine 14 BAs in fish samples. The method showed good linearity, low limits of detection, and satisfactory recovery. It was successfully applied to analyze the concentration levels of BAs in different fish.
Article
Biochemical Research Methods
Elisabet Foguet-Romero, Iris Samarra, Maria Guirro, Marc Riu, Jorge Joven, Javier A. Menendez, Nuria Canela, Antoni DelPino-Rius, Salvador Fernandez-Arroyo, Pol Herrero
Summary: Advances in metabolomics analysis and data treatment have increased our understanding of complex biological systems. Gas chromatography-mass spectrometry (GC-MS) is one of the most commonly used methods. However, method standardization and derivatization steps in GC-MS can introduce experimental errors and be time-consuming. This study proposes the injection-port derivatization (IPD) method to increase analysis throughput.
JOURNAL OF PROTEOME RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Fatima Hameedat, Sahar Hawamdeh, Soraya Alnabulsi, Aref Zayed
Summary: This review presents the HPLC-FLD methods reported in the literature for the quantification of steroids in clinical, pharmaceutical, and environmental applications, with a focus on the various approaches of fluorescent derivatization.
Article
Chemistry, Analytical
Charikleia Tziasiou, Anastasia D. Pournara, Manolis J. Manos, Dimosthenis L. Giokas
Summary: This study reports the use of a thiol-functionalized Zr-metal organic framework (MOF-SH) for the extraction of noble metal nanoparticles from environmental water samples. The MOF-SH material has high adsorption capacity and short extraction time, and can effectively extract and discriminate gold nanoparticles and gold ions, with comparable detection limits and precision to other methods.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Nazir Fattahi, Mojtaba Shamsipur, Ziba Nematifar, Nasrin Babajani, Masoud Moradi, Shahin Soltani, Shahram Akbari
Summary: This study established a pH-switchable hydrophobic deep eutectic solvent-based liquid phase microextraction (DES-LPME) technique combined with gas chromatography-mass spectroscopy for the determination of estrogenic compounds in environmental water and wastewater samples. The method is simple, fast, and exhibits low relative standard deviation and limits of detection.
Article
Biochemical Research Methods
T. Mouskeftara, C. Virgiliou, G. Theodoridis, H. Gika
Summary: The sensitive and precise GC-MS/MS method was developed for quantifying a large number of organic acids in human urine, based on key metabolic intermediates. Extensively validated, it can find wide applicability in metabolomics for clinical or nutritional studies.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Review
Instruments & Instrumentation
Kai Shi, Xiaoyan Tu, Yan Hu, Xinfeng Zhang
Summary: Thallium (Tl) pollution has become a major concern due to its high toxicity and increasing release into the environment by human activities. Accurate determination of Tl in biological and environmental samples is crucial as its bioaccumulation can lead to various diseases and even death. This review article provides an overview of different analytical methods for Tl detection, including atomic emission spectrometry, atomic absorption spectrometry, inductively coupled plasma mass spectrometry, and molecular spectroscopy. It also summarizes the current trends in sample pretreatment techniques for Tl detection in environmental and biological samples, as well as briefly discussing the speciation analysis of different Tl species.
APPLIED SPECTROSCOPY REVIEWS
(2023)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).