Review
Chemistry, Applied
Petr Tuma, Frantisek Opekar, Pavel Dlouhy
Summary: This paper provides a comprehensive survey of using capillary and microchip electrophoresis with contactless conductivity detection for the analysis of drinking water, beverages, and food items. It highlights the analysis of food on microchips and modern trends such as dual-opposite injection. CE/(CD)-D-4 is characterized by its universality, analysis speed, simple sample preparation, and minimal sample consumption.
Article
Chemistry, Analytical
Michele Alves Santana, Claudimir Lucio do Lago
Summary: There is a growing interest in conductivity detection for capillary electrophoresis, especially due to the capacitively coupled contactless conductivity approach. This method is robust and general-purpose, with sensitivity dependent only on the effective charge and effective mobility of the analyte. The technique has the potential to be used in field applications and laboratories, particularly when standard solutions are not available for unstable analytes.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Applied
Daniel Sarkozy, Beata Borza, Apolka Domokos, Eszter Varadi, Marton Szigeti, Agnes Meszaros-Matwiejuk, Dora Molnar-Gabor, Andras Guttman
Summary: This study presents an ultrafast high-resolution method for separating human milk oligosaccharides (HMOs) using multicapillary gel electrophoresis, which improves the analysis speed and resolution of HMOs.
Article
Biochemical Research Methods
Saara Mikkonen, Asa Emmer
Summary: CE-(CD)-D-4 methods were used for analyzing amino acids, successfully identifying 20 proteinogenic AAs and analyzing free AAs in samples of mammalian cell culture supernatant for the first time. By combining results from two CE methods after dilution, changes in the concentrations of 17 AAs during CHO cell cultivation could be monitored efficiently.
Article
Chemistry, Analytical
Ekaterina Yuskina, Nikodim Makarov, Maria Khaydukova, Tatiana Filatenkova, Olga Shamova, Valentin Semenov, Vitaly Panchuk, Dmitry Kirsanov
Summary: A very simple and inexpensive sensor device has been developed using an inductance coil connected to a high-frequency electric field generator. This sensor can distinguish substances with different physical and chemical properties, quantify ion concentrations in aqueous solutions and fat content in milk and cream samples, and differentiate between bacterial cultures and cell line cultures.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Petr Tuma
Summary: Contactless conductivity detection (C4D) is a universal detection technique that plays a significant role in the analysis of clinical samples. It is particularly sensitive to low molecular weight biogenic substances and can directly quantify them in their native form, making it widely applicable.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Xing Liu, Wenshan Liang, Hui Zeng, Yiyu Jiang, Yan Li, Min Zhang
Summary: The high-speed capillary electrophoresis (HSCE) method utilizes a high electric field strength applied through a short capillary to reduce sample separation time. A 3D-printed cartridge with integrated contactless conductivity detection (C4D) head and a sheath liquid channel is described to address the pronounced Joule heating effects caused by the increased electric field strength. The method allows for increased background electrolyte concentration and improved sample stacking and peak resolutions, with the ability to separate typical cations within 22 s at an applied field strength of 1200 V/cm.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Audrius Maruska, Tomas Drevinskas, Mantas Stankevicius, Kristina Bimbiraite-Surviliene, Vilma Kaskoniene, Linas Jonusauskas, Roaldas Gadonas, Staffan Nilsson, Olga Kornysova
Summary: This work describes the design and characterization of a multi-cell capacitively coupled contactless conductivity detection system, capable of simultaneously acquiring data from multiple detector cells and easily upgradable to 64 detection cells. The system demonstrated sensitivity to low concentrations of various solutions in water and the ability to distinguish different mixtures of organic solvents. It is expected to be used in conjunction with multi-channel separation devices for monitoring simultaneous processes.
ANALYTICAL METHODS
(2021)
Article
Chemistry, Analytical
Zaifang Zhu, Mark Lies, John Silzel
Summary: Using a laser driven light source for excitation, a native fluorescence detection (NFD) scheme for protein analysis was reported in this study. The results suggest that CE-NFD is a practical and powerful tool for protein characterization in the biopharmaceutical industry.
ANALYTICA CHIMICA ACTA
(2021)
Review
Biochemical Research Methods
Abdalla A. Elbashir, Abdelbagi Osman, Mohammed Elawad, Abobakr K. Ziyada, Hassan Y. Aboul-Enein
Summary: The coupling of capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C4D) is a convenient analytical method for the determination of small molecules that lack chromogenic or fluorogenic groups. This article demonstrates the use of CE-C4D in the determination of inorganic and organic ions, as well as amino acids, in the biomedical field. Attention is given to the composition of the background electrolyte, sample treatment procedures, and the utilization of multi-detection systems. Tables summarizing highly developed CE-C4D methods and the achieved figures of merit are included. Lastly, concluding remarks and perspectives are provided.
Article
Chemistry, Analytical
Wanxin Li, Jiaxin Sun, Yao Xin, Yu Han, Yanyang Sun, Aijun Li, Zhi Wang
Summary: A novel nanozyme was synthesized for the detection of resveratrol. This nanozyme exhibited excellent peroxidase-like activity and the colorimetric reaction could be completed within 20 minutes.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Multidisciplinary
Velu Duraisamy, Velayutham Sudha, Kuppusamy Annadurai, Sakkarapalayam Murugesan Senthil Kumar, Rangasamy Thangamuthu
Summary: The development of active and defective sites in mesoporous carbon materials is crucial for high-performance sensors. NHCS-1000, with improved surface area, porosity, and N-doping, showed enhanced electrocatalytic activity and was successfully utilized for the simultaneous detection of multiple analytes.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Chemistry, Analytical
Hongyu Nie, Zhihui Li, Xiaokun Wang, Rongmeng Gu, Hongyan Yuan, Yong Guo, Dan Xiao
Summary: In this study, a multi-input capacitively coupled contactless conductivity detector (MICD)-D-4 was proposed with high sensitivity, and further improved by combining it with differential detection to form (DFMICD)-D-4. The new detector showed improved performance compared to traditional single-input and double-input detectors, with a higher response-to-baseline ratio and a low limit of detection (0.7 nM). It can be applied to the detection of low-conductivity solutions and total dissolved solids (TDS) analysis, demonstrating its versatility and superiority over standard conductivity meters.
Article
Biochemical Research Methods
Alice Tomnikova, Petr Kozlik, Tomas Krizek
Summary: Saccharides play a major role in biological macromolecules and glycosylation regulates many biological processes. This study optimized a capillary electrophoresis method for separating eight commonly found monosaccharides in glycoproteins. The method showed good detection and quantification limits, and could be used as a profiling tool in glycoproteomics.
Article
Biochemical Research Methods
Jienan Li, Lin Lin, Bowei Jiang, Chudong Wang, Moutanou Modeste Judes Zeye, Dan Wen, Wei He, Weifeng Qu, Ying Liu, Lagabaiyila Zha
Summary: A Multi-InDels panel was designed for detecting highly degraded samples, capable of genotyping 18 autosomal Multi-InDels through capillary electrophoresis. The panel showed high efficiency and reliability in human identification and parentage testing.
Article
Electrochemistry
Marina Di-Oliveira, Raquel G. Rocha, Lucas V. de Faria, Eduardo M. Richter, Rodrigo A. A. Munoz
Summary: Extrusion-based 3D printing is a promising technology for constructing electrochemical devices. In this study, carbon-black integrated polylactic acid (CB/PLA) electrodes were used to detect chloramphenicol (CAP) in milk and tap water samples. The results showed that the activation of the electrode surface in a basic medium significantly improved the response to CAP.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2022)
Article
Chemistry, Analytical
Cassiano Cunha de Souza, Thalles Pedrosa Lisboa, Wallace Burger Verissimo de Oliveira, Rodrigo Alejandro Abarza Munoz, Maria Auxiliadora Costa Matos, Renato Camargo Matos
Summary: A simple and affordable method for manufacturing paper-based electrodes (PBE) using filter paper and conductive ink based on graphite and nail polish was presented in this study. Disposable sensors were applied for the first time to electrochemically monitor amoxicillin (AMX) in complex samples, such as milk, pharmaceutical formulations, and synthetic urine. The results showed that the proposed electrodes exhibited excellent electrochemical behavior, a wide linear working range, and good accuracy and precision.
Article
Agriculture, Multidisciplinary
Raquel G. Rocha, Lucas V. de Faria, Vanessa F. Silva, Rodrigo A. A. Munoz, Eduardo M. Richter
Summary: A low-cost and portable electrochemical method for detecting sulfanilamide residues in honey was developed using a 3D-printed disposable device. The method demonstrated linear concentration ranges, a low detection limit, and suitable RSD values, and it was selective for sulfanilamide in honey samples. The results obtained by the proposed method were statistically similar to those obtained by HPLC, indicating the accuracy and absence of matrix interference in the analysis.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Analytical
William B. Veloso, Vanessa N. Ataide, Diego P. Rocha, Helton P. Nogueira, Abner de Siervo, Lucio Angnes, Rodrigo A. A. Munoz, Thiago R. L. C. Paixao
Summary: The combination of CO2 laser ablation and electrochemical surface treatments enhances the electrochemical performance of 3D-printed CB/PLA electrodes by producing Na2O flower-like nanostructures on their surface. SEM images show that the treatment ablates the polymeric layer of the electrode, exposing a porous surface for the formation of Na2O nanostructures. The electrodes treated by this combined method exhibit improved performance and electrochemical response for tyrosine oxidation, making them suitable sensors for biological sample analysis, even at low analyte concentrations.
Article
Electrochemistry
Daniel S. Araujo, Luciano C. Arantes, Lucas V. Faria, Karla A. O. Souza, Dilton M. Pimentel, Sandro L. Barbosa, Eduardo M. Richter, Rodrigo A. A. Munoz, Wallans T. P. dos Santos
Summary: This study presents the electrochemical detection of 5F-MDMB-PICA, one of the most reported synthetic cannabinoids in seized forensic samples, for the first time. The method uses an adsorptive stripping differential pulse voltammetry (AdSDPV) technique with a boron-doped diamond electrode (BDDE) and demonstrates good selectivity and stability for 5F-MDMB-PICA detection. The method has a wide linear range and low limit of detection, making it suitable for fast screening and on-site application in forensic analysis.
ELECTROCHIMICA ACTA
(2023)
Article
Electrochemistry
Pedro H. S. Borges, Laiz C. D. Narciso, Guilherme Fernandes de Souza Miguel, Guedmiller Souza de Oliveira, Moacyr Comar Junior, Antonio Eduardo da Hora Machado, Rodrigo A. A. Munoz, Edson Nossol
Summary: This work evaluates the electrocatalytic oxidation of hydrazine (HDZ) by silver (AgHCF) and copper (CuHCF) hexacyanoferrates-modified glassy carbon electrodes kinetically and analytically through experimental electrochemistry and theoretical approaches. The AgHCF and CuHCF-modified electrodes showed quasi-reversible characteristics driven by K+ diffusion and adsorption processes, respectively. The CuHCF modified electrode showed superior kinetic properties and sensitivity compared to the AgHCF-modified electrode, which can be attributed to its cubic structural arrangement.
ELECTROCHIMICA ACTA
(2023)
Article
Chemistry, Applied
Gabriel Martins Fernandes, Diandra Nunes Barreto, Alex Domingues Batista, Joao Flavio da Silveira Petruci
Summary: In this study, a miniaturized, simple, and low-cost device was developed for sulfite determination in beverages. Gas Diffusion Microextraction was coupled with paper-based analytical devices, and the color change of an acid-base indicator impregnated onto the paper surface was monitored using a smartphone. The analytical information was extracted from the video file in the Hue, Saturation, Value (HSV) color space. The method showed good performance in various beverages and had a low limit of detection.
Article
Chemistry, Analytical
Murillo N. T. Silva, Raquel G. Rocha, Eduardo M. Richter, Rodrigo A. A. Munoz, Edson Nossol
Summary: A rapid and simple method for the determination of glucose was evaluated using a nanocomposite film. The film showed excellent electrocatalytic activity for the oxidation of glucose in an alkaline medium.
Article
Chemistry, Analytical
Vanderli Garcia Leal, Habdias A. Silva-Neto, Sidnei Goncalves da Silva, Wendell Karlos Tomazelli Coltro, Joao Flavio da Silveira Petruci
Summary: This study presents a quadcopter drone equipped with a fully integrated 3D-printed analytical laboratory for monitoring H2S concentration in the atmosphere. The analytical method used was based on the fluorescence quenching reaction between fluorescein mercuric acetate and H2S. The proposed device allows detection of H2S in the range of 15-200 ppbv.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Tiago A. Matias, David L. O. Ramos, Lucas V. Faria, Abner de Siervo, Eduardo M. Richter, Rodrigo A. A. Munoz
Summary: A new electrochemical device is developed by combining 3D printing and laser-generated graphene sensors. The device utilizes 3D-printed cells and electrodes, as well as laser-induced graphene, to achieve excellent electrochemical sensing properties for the detection of atropine in beverages. The method shows a linear range, low detection limit, and satisfactory precision, making it a useful portable analytical tool for on-site applications in the forensic scenario.
Article
Chemistry, Analytical
Jian F. S. Pereira, Marina Di-Oliveira, Lucas V. Faria, Pedro H. S. Borges, Edson Nossol, Rogerio V. Gelamo, Eduardo M. Richter, Osmando F. Lopes, Rodrigo A. A. Munoz
Summary: A cold CO2 plasma treatment was shown to improve the electrochemical activity of graphite sheet (GS) electrodes by increasing surface defects and oxygenated functional groups. This treatment significantly enhanced the electron transfer coefficient and reduced the resistance to charge transfer. The CO2-GS electrode demonstrated high sensitivity for the detection of emerging contaminant species in natural waters and urine samples, with submicromolar limit of detection values achieved.
Article
Chemistry, Analytical
Carlos E. C. Lopes, Lucas V. de Faria, Diele A. G. Araujo, Eduardo M. Richter, Thiago R. L. C. Paixao, Luiza M. F. Dantas, Rodrigo A. A. Munoz, Iranaldo S. da Silva
Summary: In this study, a 3D-printed electrode was manufactured using a conductive filament and used for the detection of antibiotics. By improving the electrode preparation method, the fouling phenomenon on the electrode surface was solved, and comparable analytical performance to conventional electrodes was achieved. This method is suitable for routine analysis of environmental, pharmaceutical, and food samples.
Article
Chemistry, Analytical
Alegre N. S. Cadeado, Sidnei G. G. Silva
Summary: In this work, a novel and environmentally friendly approach for quantifying glycerol in wine samples using a portable optical device based on the maker concept and do-it-yourself (DIY) principles is presented. The method involves the oxidation of glycerol using periodate, followed by the formation of the 3,5-diacetyl-1,4-dihydrolutidine (DDL) compound through a reaction with acetylacetone. A cost-effective AS7341 color sensor is used as a detector for accurate and sensitive detection of glycerol levels in wine samples. The method exhibits excellent precision and suitability for routine glycerol analysis in the wine industry, as well as addressing challenges related to wine adulteration and counterfeiting.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Multidisciplinary
Mariana C. Marra, Michelle M. A. C. Ribeiro, Rodrigo A. A. Munoz, Eduardo M. Richter
Summary: Losartan potassium and hydrochlorothiazide can be simultaneously quantified with potassium using an ultra-fast and environmentally friendly method based on capillary electrophoresis with conductivity detection. The method is simple, rapid, and accurate, and the results are comparable to those obtained by high-performance liquid chromatography and flame photometry.
JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Analytical
Charbel. N. Mouchrek, Tiago A. Matias, Lucas V. de Faria, Mariana C. Marra, Thiago R. L. da Paixa, Eduardo M. Richter, Luiza M. F. Dantas, Rodrigo A. A. Munoz, Iranaldo S. da Silva
Summary: A novel composite material using carbon black and cobalt phthalocyanine (CoPc/CB) was synthesized and characterized. The material exhibited excellent electrochemical sensing properties on a glassy carbon electrode, allowing for the detection of cysteine. The modified electrode showed significant electrocatalytic effects and enhanced peak current compared to the unmodified electrode, with a wide linear range and adequate detectability.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Qian Wu, Li Tian, Xiangyu Shan, Huiling Li, Chao Li, Juan Lu
Summary: A novel ECL sensor was developed by modifying Fe3CuO4-Ru(bpy)3+ and GO-CdS@ZnS on the electrode for the detection of permethrin. The sensor exhibited high sensitivity and a wide detection range.
Article
Chemistry, Analytical
Tingting Gong, Lei Liao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a fluorescent biosensing system for high sensitivity detection of MT was established using a new Ag+-stabilized DNA triplex probe (Ag+-SDTP) combined with catalytic hairpin assembly (CHA) and CRISPR/Cas12a signal enhancements. The sensing method is able to selectively detect MT from other non-specific molecules and can achieve low level detection of MT in diluted human serums, demonstrating its potential for monitoring disease-specific MT biomarker at trace levels.
Article
Chemistry, Analytical
Estrella Sanz Rodriguez, Kyra Wood, Paul R. Haddad, Brett Paull
Summary: This study presents a simple, rapid, and reliable method for determining boron in aqueous solutions using suppressed ion chromatography coupled to electrospray ionisation-triple quadrupole mass spectrometry. The method achieved a low limit of detection and demonstrated excellent analytical performance.
Article
Chemistry, Analytical
Aline Cournut, Paul Moustiez, Yannick Coffinier, Christine Enjalbal, Claudia Bich
Summary: Alzheimer's disease is a major cause of senile dementia and is characterized by the aggregation of neurofibrillary tangles and amyloid plaques in the brain. The A beta 1-42 peptide is considered the primary cause of neurotoxicity due to its insolubility and subsequent aggregation. A beta 1-42 and A beta 1-40 have been identified as relevant biomarkers for AD diagnosis, and SurfaceAssisted Laser Desorption/Ionization Mass Spectrometry (SALDI-MS) was used to monitor the A beta 1-42/A beta 1-40 ratio without prior sample treatment.
Article
Chemistry, Analytical
Dotse Selali Chormey, Buse Tugba Zaman, Tulay Borahan Kustanto, Sezin Erarpat Bodur, Suleyman Bodur, Elif Ozturk Er, Sezgin Bakirdere
Summary: This review provides an overview of the harmful effects of endocrine disrupting chemicals (EDCs) on human health and introduces the methods for analyzing EDCs. Recent research has focused on using green chemicals, such as deep eutectic solvents (DESs), for microextraction to ensure environmental safety.
Article
Chemistry, Analytical
Yan Zhang, Renfeng Xu, Jingjing Wu, Zhenghong Zhang, Yuhuang Wang, Hongqin Yang, Sheng Zhang
Summary: This study visualized the pore-like structures in prostate cancer cell membranes after treatment with the anticancer drug paclitaxel, demonstrating its mechanism of action related to oxidative damage.
Article
Chemistry, Analytical
Chihong Ma, Qin Zhou, Jinjin Shi, Hua Gao, Di Huang, Huimin Xue, Han Wang, Zhenzhong Zhang, Sen Yang, Junli Zhang, Kaixiang Zhang
Summary: We developed a CRISPR-empowered electrochemical biosensor, named PER-E-CRISPR, for amplification-free and sensitive detection of miR-21. The biosensor combines the advantages of CRISPR/Cas13a and primer exchange reaction (PER) for target amplification. Under optimized conditions, the PER-E-CRISPR assay exhibits a linear range from 10-13 to 10-7 M and a limit of detection of 30.2 fM. The biosensor shows excellent performance in actual plasma, indicating promising prospects for miRNA detection in molecular diagnosis.
Article
Chemistry, Analytical
Gang Liang, Jie Zhao, Yufei Gao, Tao Xie, Jianhui Zhen, Ligang Pan, Wenwen Gong
Summary: Molecular docking (MD) analysis is widely used for studying the interaction between aptamers and small molecules. This study established steady-state aptamers of tertiary structures (SATS) using specific aptamers of tetracycline antibiotics as docking models. Molecular docking results revealed multiple binding sites in the SATS of aptamers, with significant variations in binding free energy (BFE) and docking score (DS). The study also proposed a method for MD analysis based on SATS, providing insights into binding mode and predicting binding sites.
Article
Chemistry, Analytical
Yixuan Xu, Changchang Teng, Huiping Dang, Dalong Yin, Lifeng Yan
Summary: Stable organic radical molecules with high NIR fluorescence quantum efficiency have been synthesized and encapsulated into nanoparticles, which show great potential for both photodynamic therapy (PDT) and photothermal therapy (PTT) of tumors.
Article
Chemistry, Analytical
Yu Su, Dandan Yang, Yanjie Wang, Jie Ding, Lan Ding, Daqian Song
Summary: In this study, a highly selective molecularly imprinted polymer Cu-MIPs@CS was designed and synthesized for bisphenol A (BPA). Cu-MIPs@CS showed significantly enhanced imprinting factor and selective factor towards BPA compared to MIPs@CS. It also exhibited superior discrimination ability between BPA and its structural analogue. Additionally, Cu-MIPs@CS were successfully applied as a solid phase extraction adsorbent for the determination of BPA in drinking water samples.
Article
Chemistry, Analytical
Yi Qiao, Qiongdan Zhang, Yukun He, Tianguang Cheng, Jing Tu
Summary: Single cell heterogeneity plays an important role in many biological phenomena, and distinguishing cells with specific mutations is beneficial for clinical diagnosis and drug screening. This study introduces a gel plate platform that can distinguish cell types based on their phenotypes, with low equipment requirement. The gel platform has potential in point-of-care circumstances and single-cell stimulation responses that prioritize efficiency and simplicity.
Article
Chemistry, Analytical
Chengke Wang, Shuyang Sun, Ping Wang, Huawei Zhao, Wenling Li
Summary: The food safety of aquatic products is a global concern. Nanotechnology-based analyses have advantages in the detection of bacteria, metal ions, and small molecule contaminants. This review summarizes the recent advances in biosensing strategies for aquatic products and highlights the application of nanomaterials, lateral flow-based biosensors, surface-enhanced Raman scattering, microfluidic chips, and molecular imprinting technologies.
Article
Chemistry, Analytical
Liping Zhu, Zeng Tang, Xuemei Zhang, Li Zhu, Tian Meng, Linying Yu, Ting Xiao, Shasha Lu, Xiaoli Xiong, Xiurong Yang
Summary: A sensitive off-on electrochemiluminescence DNA sensor was developed using Exo III-assisted cascade amplification system. The sensor achieved high selectivity and ultra-high sensitivity in detecting target DNA by utilizing ECL and SPR effects. This study provides a new perspective for designing highly sensitive and programmable ECL biosensors.
Article
Chemistry, Analytical
Tong Li, Mengxu Sun, Suping Xia, Ting Huang, Rong-Tian Li, Chunrong Li, Zong Dai, Jin-Xiang Chen, Jun Chen, Nuan Jia
Summary: Here, we developed a binary system based on DNA tetrahedron and fluorogenic RNA aptamers for highly specific and label-free mRNA imaging in living cells. This system achieved high specificity label-free detection of nucleic acids with a detection limit of 1.34 nM. The method has potential applications in early disease diagnosis and new drug development.
Article
Chemistry, Analytical
Yao Zhao, Shu Zhang, Wang Yao, Yuxuan Zhu, Jing Qian, Juan Yang, Nianjun Yang
Summary: A new hierarchical nanostructured composite with high conductivity and catalytic activity is synthesized. The sensor exhibits significant catalytic effect and high sensitivity for monitoring the environmental endocrine disruptor bisphenol A (BPA).