Article
Chemistry, Analytical
Chao Li, Haoyu Wang, Rong Wei, Jingzhi Ren, Mengyang Zhou, Chao Yan, Lin Huang
Summary: Exosome-based liquid biopsy has great potential in tumor diagnosis and prognosis. A separable visual aptasensor for sensitive exosome detection was developed using double spherical nucleic acids (DSNAs) and terminal deoxynucleotidyl transferase (TdT).
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Multidisciplinary
Hassan H. Fakih, Adam Katolik, Elise Malek-Adamian, Johans J. Fakhoury, Sepideh Kaviani, Masad J. Damha, Hanadi F. Sleiman
Summary: Spherical nucleic acids (SNAs) show great potential as drug delivery vectors for nucleic acid therapeutics, with recent modifications enhancing stability and gene silencing potency. A cleavable DNA-based spacer is essential for maximum activity, and the modularity of FANA-SNA allows for versatile applications in gene silencing for various targets. Transfection-free delivery is superior for modified SNAs compared to free FANA oligonucleotides.
Article
Biochemical Research Methods
Yuan Zhou, Jinfu Zhang, Haowen Sun, Dagang Tao, Bingrong Xu, Xiaosong Han, Ruimin Ren, Jinxue Ruan, Lucilla Steinaa, Johanneke D. Hemmink, Jianlin Han, Xinyun Li, Jing Xu, Shuhong Zhao, Shengsong Xie, Changzhi Zhao
Summary: We developed a rapid, instrument-free, and low-cost Exo III-assisted RAA colorimetric assay-based nucleic acid detection platform for sensitive and specific detection of African swine fever virus (ASFV).
ACS SYNTHETIC BIOLOGY
(2023)
Article
Chemistry, Analytical
Kosar Shahsavar, Ehsan Shokri, Morteza Hosseini
Summary: A new colorimetric platform based on spherical nucleic acid (SNA) and G-quadruplex DNAzymes has been developed for rapid and sensitive detection of specific miRNAs. The platform utilizes hybridization between target miRNA and capture probes to achieve detection. The method is capable of detecting miRNA-155 at concentrations as low as 0.7 nM and can be applied to real human serum samples.
Article
Chemistry, Analytical
Fang Liu, Ru Chen, Wenlu Song, Liangwen Li, Chunyang Lei, Zhou Nie
Summary: This study proposed a colorimetric protease assay by combining proteolysis and RNA synthesis to trigger SNAs aggregation, achieving sensitive and specific detection of MMP-2. The method's applicability can be extended to other protease biomarkers and can enable portable detection with smartphone, highlighting its potential for point-of-care diagnosis of diseases.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Chao Li, Long Guo, Xiaoqiang Sang, Xiaoli Jiang, Hanwen Wang, Panzhu Qin, Lin Huang
Summary: In this study, a colorimetric aptasensor platform was developed by integrating aptamer-based spherical nucleic acids (SNAs) and hybridization chain reaction (HCR) for exosome detection. The CD63-specific aptamer captured target exosomes, while SNAs with nucleolin-specific aptamer and trigger probe H1 amplified the signal through HCR. The proposed aptasensor achieved a low detection limit of 50 particles/μL and showed practicality in testing different clinical samples, suggesting its potential as an on-site test for liquid biopsy.
Article
Biochemical Research Methods
Fuming Sang, Suyao Yin, Jianxin Pan, Zhizhou Zhang
Summary: The novel aptasensor combines the plasmonic properties of gold nanoparticles (AuNPs) with exonuclease III (Exo III)-assisted target recycling for ultrasensitive detection of Hg2+, with a linear range from 5 pM to 0.6 nM and a detection limit of 0.2 pM. The sensor allows visual detection of Hg2+ as low as 50 pM by the naked eye and reliable determination of spiked Hg2+ in sea water samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Applied
Jingwen Li, Yongming Liu, Hao Lin, Yan Chen, Zhenbo Liu, Xuming Zhuang, Chunyuan Tian, Xiuli Fu, Lingxin Chen
Summary: A label-free colorimetric method based on Exo I-assisted signal amplification with protamine as a medium was developed for analysis of kanamycin. The method, which involved manipulating a dsDNA probe tailored from an aptamer and cDNA, demonstrated high selectivity and sensitivity for target detection. The strategy showed great potential for fabricating various biosensors based on target-induced aptamer configuration changes.
Article
Chemistry, Analytical
Hongfei He, Yan Zhou, Bin Chen, Yi Zhang, Xiaowu Zhong, Lei Xu, Bin Guo, Chong Yin, Xi Zhou, Qingrong Li, Zhen Huang, Guangcheng Luo, Xiaolan Guo
Summary: A colorimetric isothermal sensing platform (SFMC) with specific signal filtration and magnification has been developed for ultrasensitive detection of DNA and RNA. This platform can detect target DNA as low as 5 copies per reaction and target RNA as low as 10 copies per reaction by naked eyes. It has also been successfully used for the detection of African swine fever virus (ASFV) and SARS-CoV-2.
Article
Biotechnology & Applied Microbiology
Peter Q. Nguyen, Luis R. Soenksen, Nina M. Donghia, Nicolaas M. Angenent-Mari, Helena de Puig, Ally Huang, Rose Lee, Shimyn Slomovic, Tommaso Galbersanini, Geoffrey Lansberry, Hani M. Sallum, Evan M. Zhao, James B. Niemi, James J. Collins
Summary: This study demonstrates the development of wearable devices functionalized with freeze-dried, cell-free synthetic circuits for noninvasive detection of biomolecules, including virus nucleic acids, without the need for living engineered bacteria. The technology shows promise for expanding opportunities in physiological status monitoring and disease detection.
NATURE BIOTECHNOLOGY
(2021)
Article
Chemistry, Analytical
Deyan Xu, Zheng Shen, Guangxian Wang, Lin Wei, Xiaolin Gao, Haowei Dong, Guanjie Wang, Xia Sun, Falan Li, Yemin Guo
Summary: Colorimetry is limited by low sensitivity and high background signal in its application. This study developed a Fe@Co-N stellate porous carbon nanoenzyme with a large specific surface area, which contained Fe-N and cobalt double peroxide active sites. A colorimetric biosensor with a low background signal and ultra-sensitive detection of tetracyclines based on a shared structure was successfully constructed. The developed strategy achieved highly sensitive detection of low concentrations of antibiotics, with a limit of detection (LOD) of 0.333 ng/mL (S/N = 3), indicating its potential for simultaneous detection of multiple tetracyclines.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Review
Chemistry, Analytical
Jingjing Li, Yuanyuan Li, Limeng Pan, Wei Pan, Na Li, Bo Tang
Summary: This study summarizes the recent advances and applications of spherical nucleic acid (SNAs)-based biosensors for tracking and imaging cancer-related biomarkers, and discusses the future challenges and opportunities of SNAs for cancer diagnosis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Kunlun Jiang, Jingrui Wu, Ji-Eun Kim, Sujin An, Jwa-Min Nam, Yung-Kang Peng, Jung-Hoon Lee
Summary: A novel plasmonic cross-linking colorimetric PCR (PPTccPCR) assay is proposed by integrating plasmonic magnetic nanoparticle (PMN)-based PPT-PCR with gold nanoparticle (AuNP) cross-linking colorimetry. This method enables visual detection of target DNA with concentrations as low as 5 copies/μL within 40 minutes. The simple and sensitive strategy does not require expensive instrumentation and has high potential for clinical diagnostic applications.
Article
Chemistry, Analytical
Samira Balbach, Nan Jiang, Rosalia Moreddu, Xingchen Dong, Wolfgang Kurz, Congyan Wang, Jie Dong, Yixia Yin, Haider Butt, Martin Brischwein, Oliver Hayden, Martin Jakobi, Savas Tasoglu, Alexander W. Koch, Ali K. Yetisen
Summary: Colorimetric tests for at-home health monitoring have become popular due to their reduced costs and ease of operation, but developing digital systems to interface these sensors remains a challenge. Efforts have been made to develop portable optical readout systems, such as smartphones, but their use in daily settings is limited by optical noise and low sensitivity.
ANALYTICAL METHODS
(2021)
Article
Chemistry, Physical
Baiqiang Zhai, Kunlun Huang, Hongtao Wang, Dongmin Su, Yuancong Xu
Summary: A dual-DNAzyme colorimetric biosensor was developed using Cu-DNAzyme and G4-DNAzyme for the detection of Cu2+ through exonuclease III-mediated cyclical assembly. By adjusting metal-dependent DNA-cleaving DNAzymes, this biosensor can detect any metal ion target, which is significant for rapid determination of food safety.
Article
Chemistry, Analytical
Abbas Karami, Masoumeh Hasani
ANALYTICA CHIMICA ACTA
(2020)
Article
Chemistry, Analytical
Abbas Karami, Masoumeh Hasani, Farid Azizi Jalilian, Razieh Ezati
Summary: This study modified the PCR assay with target-responsive assembly of gold nanoparticle-core hairpin-spherical nucleic acids for naked-eye colorimetric detection of SARS-CoV-2 RNA, achieving similar sensitivity to real-time RT-PCR method.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Zahra Souri, Saber Alizadeh, Davood Nematollahi, Mohammad Mazloum-Ardakani, Abbas Karami
Summary: In this study, a green and template-free strategy for the electropolymerization of imipramine was developed, creating sponge-like film architectures on the electrode. The modified electrode was characterized using various techniques and evaluated for its electrochemical performance.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Abbas Karami, Masoumeh Hasani
Summary: This study presents a new biosensing platform called nano-sweeping for quantitative detection of miRNA-30a. The method utilizes gold nanoparticle-core spherical nucleic acid hybridization and gravitational separation to achieve rapid and accurate quantification of miRNA-30a. The approach is fluorophore independent, allowing for attachment of any fluorescent dye, and has a linear response within a concentration range of 0-3.55 ng/μL.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Mengmeng Guo, Na Luo, Yueling Bai, Zhenggang Xue, Qingmin Hu, Jiaqiang Xu
Summary: A porous heterostructure WO3-C/In2O3 was designed and prepared for a miniature H2 sensor, which showed higher response value, lower operating temperature, fast response-recovery speed, and low limit of detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Feng Hu, Hui Hu, Yuting Li, Xiaohui Wang, Xiaowen Shi
Summary: Arsenic contamination in water bodies is a significant health risk. This study developed a chitosan-catechol modified electrode for rapid and accurate detection of trace amounts of arsenic. The modified electrode demonstrated good detection capability and resistance to ionic interference, making it suitable for in situ detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Yantao Zhang, Qian Liu, Tao Tian, Chunhua Xu, Pengli Yang, Lianju Ma, Yi Hou, Hui Zhou, Yongjun Gan
Summary: In this study, a lysosome-targeting buffering fluorogenic probe (Lyso-BFP) was designed and synthesized, demonstrating excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells. The performance of Lyso-BFP in pH sensing was attributed to the inhibition of the photo-induced electron transfer process. Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Wei Cai, Wenbo Sun, Jiayue Wang, Xiaokui Huo, Xudong Cao, Xiangge Tian, Xiaochi Ma, Lei Feng
Summary: In this study, a near-infrared fluorescent probe HCBG was developed for imaging of alpha-GLC. HCBG exhibited excellent selectivity and sensitivity towards alpha-GLC in complex bio-samples, and showed good cell permeability for in situ real-time imaging. Through the high-throughput screening system established by HCBG, a natural alpha-GLC inhibitor was successfully isolated and identified. This study provides a novel fluorescence visualization tool for discovering and exploring the biological functions of diabetes-related gut microbiota, and a high-throughput screening approach for alpha-GLC inhibitor.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Trey W. Pittman, Xi Zhang, Chamindie Punyadeera, Charles S. Henry
Summary: Heart failure is a growing epidemic and a significant clinical and public health problem. Researchers have developed a portable and affordable diagnostic device for heart failure that can be used at the point-of-care, providing a valid alternative to current diagnostics approaches.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Anders O. Tjell, Barbara Jud, Roland Schaller-Ammann, Torsten Mayr
Summary: An optical hydrogen peroxide sensor based on catalytic degradation and the detection of produced oxygen is presented. The sensor offers higher resolution and better sensitivity at lower H2O2 concentrations. By removing O2 from the sample solution, a more sensitive O2 sensor can be used for measurement. The sensor has been successfully applied in a flow-through cell to measure H2O2 concentration in different flow rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Seong Jae Kim, Ji-hun Jeong, Gaabhin Ryu, Yoon Sick Eom, Sanha Kim
Summary: Surface-enhanced Raman spectroscopy (SERS) is a high-sensitivity, label-free detection method with various analytical applications. Researchers have developed a hydrophobic SERS substrate based on engineered carbon nanotube arrays (CNT-SERS) and studied the role of structural design at both micro and nanoscales. The substrate demonstrated controlled self-enrichment capability and enhanced sensitivity, with a significant increase in the SERS signal. The study also proposed a theoretical model and a concentration strategy inspired by plants for analyte deposition on microarrays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Dan Zhao, Renjun Jiang, Xiaoqiang Liu, Subbiah Alwarappan
Summary: In this study, a novel ternary composite material was constructed by assembling cerium vanadate nanorods on reduced graphene oxide-microcrystalline cellulose nanosheets, and it was used for real-time monitoring of the concentration of superoxide anions in vivo. The ternary composite showed excellent conductivity, large surface area, and abundant active sites, leading to a wider linear range, high sensitivity, low detection limit, and fast response time for superoxide anion detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Tengfei Wang, Liwen Wang, Guang Wu, Dating Tian
Summary: In this study, a covalent organic framework material TaTp-COF with porous and uniform spheres was successfully prepared via hydrothermal reaction, and it was found to significantly enhance the aggregation-induced emission (AIE) of berberine. The unique emission properties of berberine on TaTp-COF were studied and utilized for the sensitive detection of berberine.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Fangju Chen, Xueting Wang, Wei Chen, Chenwen Shao, Yong Qian
Summary: Lung cancer is the second most common malignant tumor worldwide. Drug resistance in lung cancer leads to treatment failure and recurrence in majority of patients. This study developed a fluorescent prodrug that can be activated in cancer cells to release drugs, and its signal can be tracked by imaging. It shows a unique autophagy-driven ferroptosis effect, indicating its potential for targeting drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Weichao Li, Qiming Yuan, Zhangcheng Xia, Xiaoxue Ma, Lifang He, Ling Jin, Xiangfeng Chu, Kui Zhang
Summary: This study developed a high-performance gas sensor for formaldehyde detection by modifying ZnSnO3 with ZnO QDs and SnO2 QDs. The modified sensor showed improved sensing response and lower working temperature. The presence of ZnO QDs formed rich heterojunctions, increased surface area, and provided oxygen deficiency for formaldehyde sensing reaction, thus enhancing the sensor performance. This research provides an alternative method to enhance the sensing properties of MOS by QDs modification.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Joung-Il Moon, Eun Jung Choi, Younju Joung, Jin-Woo Oh, Sang-Woo Joo, Jaebum Choo
Summary: A novel nanoplasmonic substrate was developed for biomedical applications, which showed strong hot spots for detecting biomarkers at low concentrations. The substrate, called AuNPs@M13, was made by immobilizing 60 nm gold nanoparticles onto the surface of an M13 bacteriophage scaffold. It demonstrated higher sensitivity and lower limit of detection compared to commercially available assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ning Li, Ya Zhang, Ying Xu, Xiaofang Liu, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: The molecular subtype of breast cancer guides treatment and drug selection. Invasive tests can promote cancer cell metastasis, so the development of high-performance, low-cost diagnostic tools for cancer prognosis is crucial. Liquid biopsy techniques enable noninvasive, real-time, dynamic, multicomponent, quantitative, and long-term observations at the cellular, genetic, and molecular levels. A Cu-Zr metal-organic framework (MOF) nanoenzyme with monatomic Cu attachment has been synthesized and proven to have high catalytic performance. The sensor constructed using this nanoenzyme shows potential for accurate classification of breast cancer serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Jeongmin Kim, Hyemin Kim, Seunghyun Park, Hyeonaug Hong, Yong Jae Kim, Jiyong Lee, Jaeho Kim, Seung-Woo Cho, Wonhyoung Ryu
Summary: This study presents a method to fabricate independently functioning microneedle (MN) electrodes with narrow intervals for high precision electrochemical sensing. The optimized mixture of photocurable polymer and single-wall carbon nanotubes was used to mold single composite MNs, which were then attached to pre-patterned electrodes. Plasma etching and electropolymerization were performed to enhance the electrochemical activity, and Prussian blue and glucose oxidase were electrodeposited on the MNs for glucose detection. The MN electrodes showed good sensitivity and linearity, and the feasibility of glucose detection was demonstrated in an in vivo mouse study.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)